Probable E3 ubiquitin-protein ligase MYCBP2

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Preferred order of sections

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Preferred order of sections

With

Entry

#Exp.

IntAct

Notes

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Protein names

Recommended name:
Probable E3 ubiquitin-protein ligase MYCBP2
EC=6.3.2.-

Alternative name(s):
Myc-binding protein 2
Pam/highwire/rpm-1 protein
Protein associated with Myc

Gene names

Name:	Mycbp2
Synonyms:	Pam, Phr1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Sequence length	4711 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Function	Probable E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. May have a role during synaptogenesis; candidate for respiratory distress and ventilatory disorders that arise from defective neuronal control of breathing. May function as a facilitator or regulator of transcriptional activation by MYC. Ref.1
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with MYC. Interacts with TSC2 (tuberin) when TSC2 is in complex with TSC1 (hamartin) By similarity. Interacts with FBXO45. Ref.5 UniProtKB O75592
Subcellular location	Nucleus By similarity UniProtKB O75592.
Tissue specificity	Dynamically expressed in embryonic nervous system from E8.5 through E18.5. During postnatal development, expression is particularly strong in the cerebellum, hippocampus and retina. Lower levels of expression are observed throughout the cerebral cortex. Ref.1
Domain	The PHR domains are compact beta-sandwich folds composed of 11 antiparallel strands and decorated with conserved apical loops. They are likely to play a structural role and mediate interactions with substrates or partners. Ref.6
Disruption phenotype	Mice display incomplete innervation of the diaphragm by the phrenic nerve. Intercostal muscles are completely innervated, but show dysmorphic nerve terminals. Sensory neuron terminals in the diaphragm are abnormal and neuromuscular junctions show excessive sprouting of nerve terminals, consistent with inadequate presynaptic stimulation of the muscle. Ref.1
Sequence similarities	Belongs to the highwire family. Contains 1 B box-type zinc finger. Contains 1 DOC domain. Contains 1 filamin repeat. Contains 5 RCC1 repeats. Contains 1 RING-type zinc finger.

Keywords
Biological process	Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
PTM	Disulfide bond Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	branchiomotor neuron axon guidance Inferred from mutant phenotype PubMed 18031680. Source: MGI central nervous system projection neuron axonogenesis Inferred from mutant phenotype PubMed 18031680. Source: MGI regulation of cytoskeleton organization Inferred from mutant phenotype PubMed 18031680. Source: MGI regulation of protein localization Inferred from mutant phenotype PubMed 18031680. Source: MGI regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	axon Inferred from direct assay PubMed 18031680. Source: MGI microtubule cytoskeleton Inferred from direct assay PubMed 18031680. Source: MGI nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ubiquitin-protein ligase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

With	Entry	#Exp.	IntAct	Notes
Slc12a5	Q63633	4	EBI-1811542,EBI-1811510	From a different organism.

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 4711

4711

Probable E3 ubiquitin-protein ligase MYCBP2

PRO_0000055964

Repeat

562 – 617

56

RCC1 1

Repeat

661 – 717

57

RCC1 2

Repeat

869 – 919

51

RCC1 3

Repeat

920 – 971

52

RCC1 4

Repeat

973 – 1028

56

RCC1 5

Repeat

2293 – 2400

108

Filamin

Domain

3751 – 3929

179

DOC

Zinc finger

4461 – 4512

52

RING-type; atypical

Zinc finger

4615 – 4665

51

B box-type

Region

1197 – 1348

152

PHR domain 1

Region

1685 – 1843

159

PHR domain 2

Compositional bias

65 – 88

24

Lys-rich

Compositional bias

728 – 776

49

Cys-rich

Compositional bias

2754 – 2878

125

Ser-rich

Compositional bias

2847 – 2955

109

Pro-rich

Compositional bias

3228 – 3248

21

Lys-rich

Compositional bias

3296 – 3327

32

Gly-rich

Modified residue

140

1

Phosphoserine By similarity

Modified residue

143

1

Phosphoserine By similarity

Modified residue

145

1

Phosphoserine By similarity

Modified residue

1583

1

Phosphoserine Ref.4

Modified residue

2823

1

Phosphoserine By similarity

Modified residue

2954

1

Phosphoserine By similarity

Modified residue

3124

1

Phosphoserine By similarity

Modified residue

3512

1

Phosphoserine By similarity

Modified residue

3539

1

Phosphoserine By similarity

Modified residue

3954

1

Phosphothreonine Ref.4

Disulfide bond

1707 ↔ 1822

Probable

Alternative sequence

3972 – 3974

3

Missing in isoform 2.

VSP_014184

Sequence conflict

3647

1

G → E in AAH59257. Ref.3

Sequence conflict

4598

1

E → G in AAH59257. Ref.3

1

.

4711

Helix Strand Turn

Details...

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 03C9A3975EED7552 Show »	FASTA	4,711	517,738
10 20 30 40 50 60 MPVPDGSVAA AGLGLGLPTT DSRGHYQLLL SGRALADRYR RIYTTALSDR DQAGSSTGHP 70 80 90 100 110 120 ASRNKKILNK KKLKRKQKSK SKVKTRSKSE NVENTVIIPD IKLHSNPSAF NIYCNVRHCV 130 140 150 160 170 180 LEWQKKETSL AAASKNSVQS GESDSDEEEE SREPPIKLPK IIEVGLCEVF ELIKETRFSH 190 200 210 220 230 240 PSLCLRSLQA LLNVLQGQQP EGLQSEPPEV LESLFQLLLE ITVRSTGMND STGQSLTALS 250 260 270 280 290 300 CACLFSLVAS WGETGRTLQA ISAILTNNGS HACQTIQVPT ILNSLQRSVQ AVLVGKIQVQ 310 320 330 340 350 360 DWFSNGIKKA ALMHKWPLKE VSVDEDDQCL LQNDGFFLYL LCKDGLYKIG SGYSGTVRGH 370 380 390 400 410 420 IYNSTSRIRN RKEKKSWLGY AQGYLLYRDL NNHSMTAIRI SPETLEQDGT VLLPDCHTEG 430 440 450 460 470 480 QNILFTDGEY INQIAASRDD GFVVRIFATS TEPVLQQELQ LKLARKCLHA CGISLFDLEK 490 500 510 520 530 540 DLHIISTGFD EESAILGAGR EFALMKTANG KIYYTGKYQS LGIKQGGPSA GKWVELPITK 550 560 570 580 590 600 SPKIVHFSVG HDGSHALLVA EDGSVFFTGS ASKGEDGEST KSRRQSKPYK PKKIIKMEGK 610 620 630 640 650 660 IVVYTACNNG SSSVISKDGE LYMFGKDAIY SDSSSLVSDL KGHFVTQVAM GKAHTCVLMK 670 680 690 700 710 720 NGEVWTFGVN NKGQCGRDTG AMNQGGKGFG VENMATAMDE DLEEELDEKD EKSMMCPPGM 730 740 750 760 770 780 HKWKLEQCMV CTVCGDCTGY GASCVSSGRP DRVPGGICGC GSGESGCAVC GCCKACAREL 790 800 810 820 830 840 DGQEARQRGI LDAVKEMIPL DLLLAVPVPG VNIEEHLQLR QKEKRQRVIR RHRLEDGRGP 850 860 870 880 890 900 LVFAGPIFMN HREQALARLR SHPAQLKHKR DKHKDGSGDR GEKDASKITT YPPGSVRFDC 910 920 930 940 950 960 ELRAVQVSCG FHHSVVLMEN GDVYTFGYGQ HGQLGHGDVN SRGCPTLVQA LPGPSTQVTA 970 980 990 1000 1010 1020 CSNHTAVLLM DGQVFTFGSF SKGQLGRPIL DIPYWNAKPA PMPNIGSKYG RKATWIGASG 1030 1040 1050 1060 1070 1080 DQTFLRIDEA LINSHVLATS EIFASKHIIG LVPASISEPP PFKCLLINKV DGSCKTFNDS 1090 1100 1110 1120 1130 1140 EQEDLQGFGV CLDPVYDVLW RFRPSTRELW CYNAVVADAR LPSATDMQSR CSILSPELAL 1150 1160 1170 1180 1190 1200 PTGSRALTTR SHAALHILGC LDTLAAMQDL KMGIASTEEE TQAVMKVYSK EDYSVVNRFE 1210 1220 1230 1240 1250 1260 SHGGGWGYSA HSVEAIRFSA DTDILLGGLG LFGGRGEYTA KIKLFELGPD GGDHETDGDL 1270 1280 1290 1300 1310 1320 LAETDVLAYD CAAREKYAMM FDEPVLLQAG WWYVAWARVS GPSSDCGSHG QASITTDDGV 1330 1340 1350 1360 1370 1380 IFQFKSSKKS NNGTDVNAGQ IPQLLYRLPT SDGSTSKGKQ QTSEPVHILK RSFARTVSVE 1390 1400 1410 1420 1430 1440 CFESLLSILH WSWTTLVLGV EELRGLKGFQ FTATLLDLER LRFVGTCCLR LLRVYTCEIY 1450 1460 1470 1480 1490 1500 PVSATGKAVV EETSKLAECI GKTRTLLRKI LSEGVDHCMV KLDNDPQGYL SQPLRLLEAV 1510 1520 1530 1540 1550 1560 LQECHNTFTA CFHSFYPTPA LQWACLCDLL NCLDQEANFK TSSSRLLAAV MSALCHTSVK 1570 1580 1590 1600 1610 1620 LTSLFPIAYD GEVLLRSIVK QVSTENDSTL VHRFPLLVGH MEKLSQSEEN ISGMTSFREV 1630 1640 1650 1660 1670 1680 LEKMLVIVVL PVRNSLRRES ELFSSHLVSN TCGLLASIVS ELTASALGSE VDGLNSLHSV 1690 1700 1710 1720 1730 1740 KASANRFTKT SQGRSWNTGN GSPDAICFAV DKPGIVVVGF AVYGGGGIHE YELEVLVDDS 1750 1760 1770 1780 1790 1800 EHAGDSTHSH RWTSLELVKG TYTTDDSPSD IAEIRLDKVV PLKENVKYAV RLSNYGSRTA 1810 1820 1830 1840 1850 1860 NGDGGMTTVQ CPDGVTFTFS TCSLSSNGTN QTRGQIPQIL YYRSEFDGDL QSQLLSKANE 1870 1880 1890 1900 1910 1920 EDKNCSRALS VVSTVVRAAK DLLHRALAVD ADDIPELLSS SSLFSMLLPL IIAYIGPVAA 1930 1940 1950 1960 1970 1980 AIPKVAVEVF GLVQQLLPSV AILNQKYASP AFNPNQSTDS TTGNQPEQGL SACTTSNHYA 1990 2000 2010 2020 2030 2040 VIESEHPYKP ACVMHYKVTF PECVRWMTIE FDPQCGTAQS EDVIRLLIPV RTIQNSGYGA 2050 2060 2070 2080 2090 2100 KLTSVHENLN SWVELKKYSG SSGWPTMVLV LPGNEALFSL ETASDYVKDD KASFYGFKCF 2110 2120 2130 2140 2150 2160 AIGYEFSPGP DEGVIQLEKE LANLGGVCAA ALMKKDLALP VGNELEEDLE ILEEAALQVC 2170 2180 2190 2200 2210 2220 KTHSGILGKG LALSHSPTIL EALEGNLPLQ IQSNEQSFLD DFIACVPGSS GGRLARWLQP 2230 2240 2250 2260 2270 2280 DSYADPQKTS LILNKDDIRC GWPTTITVQT KDQYGDVVHV PNMKVEVKAV PVSQKKTSLQ 2290 2300 2310 2320 2330 2340 QDQGKKCQRI PGSPSAAASS ADMTFGGLAS PKLDVSYEPM IVKEARYIAI TMMKVYENYS 2350 2360 2370 2380 2390 2400 FEELRFASPT PKRPSENMLI RVNNDGTYCA NWTPGAIGLY TVHVTIDGIE IDAGLEVKVK 2410 2420 2430 2440 2450 2460 DPPKGMIPPG TQLVKPKADP QPNKIRKFVA KDSAGLRIRS HPSLQSEQIG IVRVNGTITF 2470 2480 2490 2500 2510 2520 IDEIHNDDGV WLRLNEETIK KYVPNMNGYT EAWCLSFNQH LGKSLLVPVD NIFNASQGVR 2530 2540 2550 2560 2570 2580 DLDVFSWTSK AFFPQEPKTN TDDFFKDMNS CGPQEATMQE RDHPFLRGGP GMYKVVKTGP 2590 2600 2610 2620 2630 2640 SGHNIRSCPN LRGIPIGMLV LGNKVKAVGE VTNSEGAWVQ LDKNSMVEFC ESDEGEAWSL 2650 2660 2670 2680 2690 2700 ARDRGGNQYL RHEDEQVLLD QNSQPPPPSP FSVQAFNKGA SCSAQGFDYG LGNNKGDQLS 2710 2720 2730 2740 2750 2760 AILNSIQSRP NLPAPSIFDQ AAKPPSSLVH SPFVFGQPLS FQQRQLQSDR GTISTSSRPV 2770 2780 2790 2800 2810 2820 STSGKSELPS KHSRSVKPDG HVSRTPADQK KPRGTEGLSA SESLMLKSDA AKLRSDSHSR 2830 2840 2850 2860 2870 2880 SLSPNHNTLQ TLKSDGRTSS GFRAESPGPG SRSSSPKPKP LPTPRSSPSG ASSPRSSSPQ 2890 2900 2910 2920 2930 2940 DKNLPQKSTA PAKTKLDPPR ERSKSDSYTL DPDTLRKKKM PLTEPLRGRS TSPKPKPVPK 2950 2960 2970 2980 2990 3000 DPKDSPGSEN RAPSPHVVQE NLHSEVVEVC TSSTLKTNGV TDSTCDDSGD LKSVDEGSNK 3010 3020 3030 3040 3050 3060 VHFSIGKAPL KDEQEMRASP KISRKCANRH TRPKKEKSNF LFKGDGTKSL EPAKQAMSPS 3070 3080 3090 3100 3110 3120 VAECARAVFA SFLWHEGIVH DAMACSSFLK FNPDLSKEHA PIRSSLNSQP PTEEKEIKLK 3130 3140 3150 3160 3170 3180 NRHSLEISSA LNMFNIAPHG PDISKMGSIN KNKVLSMLKE PPLHEKCEDG KSEATFEMSM 3190 3200 3210 3220 3230 3240 HHTMKSKSPL PLTLQHLVAF WEDISLATIK AASQNMIFPS PGSCAVLKKK ECEKENKKTK 3250 3260 3270 3280 3290 3300 KEKKKKEKTE IRPRGNLFGE MAQLAVGGPE KDTICELCGE SHPYPVTYHM RQAHPGCGRY 3310 3320 3330 3340 3350 3360 AGGQGYNSIG HFCGGWAGNC GDGGMGGSTW YLVCDRCREK YLREKQAAAR EKVKQSRRKP 3370 3380 3390 3400 3410 3420 MQVKTPRALP TMEAHQVIKA NALFLLSLSS AAEPSILCYH PAKPFQSQLP IVKEGVSEDL 3430 3440 3450 3460 3470 3480 PVKMPCLYLQ TLARHHHENF VGYQDDNLFQ DEMRYLRSTS VPAPYISVTP DASPNVFEEP 3490 3500 3510 3520 3530 3540 ESNMKSMPPS LETSPITDTD LAKRTVFQRS YSVVASEYDK QHSILPARVK AIPRRRVNSG 3550 3560 3570 3580 3590 3600 DTVGSSLLRH PSPELSRLIS AHSSLSKGER NFQWPVLAFV IQHHDLEGLE IAMKQALRKS 3610 3620 3630 3640 3650 3660 ACRVFAMEAF NWLLCNVIQT TSLHDILWHF VAALTPSPVE AEEDEDGDNK SNKENAEQEK 3670 3680 3690 3700 3710 3720 DTRVCEHPLS DIVIAGEAAH PLPHTFHRLL QTISDLMMSL PSGSSLQQMA LRCWSLKFKQ 3730 3740 3750 3760 3770 3780 SDHQFLHQSN VFHHINNILS KSDDGDSEES FSISVQSGFE AMSQELCIVM CLKDLTSIVD 3790 3800 3810 3820 3830 3840 IKTSSRPAMI GSLTDGSTET FWESGDEDKN KTKNITINCV KGINARYVSV HVDNSRDLGN 3850 3860 3870 3880 3890 3900 KVTSMTFLTG KAVEELCRIK QVDLDSRHIG WVTSELPGGD NQIIKIELKG PENTLRVRQV 3910 3920 3930 3940 3950 3960 KVLGWKDGES TKIAGQISAS VAQQRSCEAE TLRVFRLITS QVFGKLISGD AEPTPEQEEK 3970 3980 3990 4000 4010 4020 ALLSSPEGEE KVYNATSDAD LKEHMVGIIF SRSKLTNLQK QVCAHIVQAI RMEATRVREE 4030 4040 4050 4060 4070 4080 WEHAISSKEN ANSQPSDEDA SSDAYCFELL SMVLALSGSN VGRQYLAQQL TLLQDLFSLL 4090 4100 4110 4120 4130 4140 HTASPRVQRQ VTSLLRRVLP EVTPNRLASI IGVKSLPPAD ISDIIHSTEK GDWNKLGILD 4150 4160 4170 4180 4190 4200 MFLGCIAKAL TVQLKAKGTT ITGTAGTTVG KGVTTVTLPM IFNSSYLRRG ESHWWMKGST 4210 4220 4230 4240 4250 4260 PTQISEIIIR LIKDMAAGHL SEAWSRVTKN AIAETIIALT KMEEEFRSPV RCIATTRLWL 4270 4280 4290 4300 4310 4320 ALASLCVLDQ DHVDRLSSGR WMGKDGQQKQ MPMCDNHDDG ETAAIILCNI CGNLCTDCDR 4330 4340 4350 4360 4370 4380 FLHLHRRTKT HQRQVFKEEE EAIKVDLHEG CGRTKLFWLM ALADSKTMKA MVEFREHTGK 4390 4400 4410 4420 4430 4440 PTTSSSEACR FCGSRSGTEL SAVGSVCSDA DCQEYAKIAC SKTHPCGHPC GGVRNEEHCL 4450 4460 4470 4480 4490 4500 PCLHGCDKSA TTLKQDADDM CMICFTEALS AAPAIQLDCS HVFHLQCCRR VLENRWLGPR 4510 4520 4530 4540 4550 4560 ITFGFISCPI CKNKINHIVL KDLLDPIKEL YEDVRRKALM RLEYEGLHKS EAITTPGVRF 4570 4580 4590 4600 4610 4620 YNDAAGYAMN RYAYYVCYKC RKAYFGGEAR CDAEAGQEDD YDPRELICGA CSDVSRAQMC 4630 4640 4650 4660 4670 4680 PKHGTDFLEY KCRYCCSVAV FFCFGTTHFC NACHDDFQRM TSIPKEELPH CPAGPKGKQL 4690 4700 4710 EGTECPLHVV HPPTGEEFAL GCGVCRNAHT F « Hide
Isoform 2 [UniParc]. Checksum: B9E8717F81DFD5F8 Show »	FASTA	4,708	517,361
10 20 30 40 50 60 MPVPDGSVAA AGLGLGLPTT DSRGHYQLLL SGRALADRYR RIYTTALSDR DQAGSSTGHP 70 80 90 100 110 120 ASRNKKILNK KKLKRKQKSK SKVKTRSKSE NVENTVIIPD IKLHSNPSAF NIYCNVRHCV 130 140 150 160 170 180 LEWQKKETSL AAASKNSVQS GESDSDEEEE SREPPIKLPK IIEVGLCEVF ELIKETRFSH 190 200 210 220 230 240 PSLCLRSLQA LLNVLQGQQP EGLQSEPPEV LESLFQLLLE ITVRSTGMND STGQSLTALS 250 260 270 280 290 300 CACLFSLVAS WGETGRTLQA ISAILTNNGS HACQTIQVPT ILNSLQRSVQ AVLVGKIQVQ 310 320 330 340 350 360 DWFSNGIKKA ALMHKWPLKE VSVDEDDQCL LQNDGFFLYL LCKDGLYKIG SGYSGTVRGH 370 380 390 400 410 420 IYNSTSRIRN RKEKKSWLGY AQGYLLYRDL NNHSMTAIRI SPETLEQDGT VLLPDCHTEG 430 440 450 460 470 480 QNILFTDGEY INQIAASRDD GFVVRIFATS TEPVLQQELQ LKLARKCLHA CGISLFDLEK 490 500 510 520 530 540 DLHIISTGFD EESAILGAGR EFALMKTANG KIYYTGKYQS LGIKQGGPSA GKWVELPITK 550 560 570 580 590 600 SPKIVHFSVG HDGSHALLVA EDGSVFFTGS ASKGEDGEST KSRRQSKPYK PKKIIKMEGK 610 620 630 640 650 660 IVVYTACNNG SSSVISKDGE LYMFGKDAIY SDSSSLVSDL KGHFVTQVAM GKAHTCVLMK 670 680 690 700 710 720 NGEVWTFGVN NKGQCGRDTG AMNQGGKGFG VENMATAMDE DLEEELDEKD EKSMMCPPGM 730 740 750 760 770 780 HKWKLEQCMV CTVCGDCTGY GASCVSSGRP DRVPGGICGC GSGESGCAVC GCCKACAREL 790 800 810 820 830 840 DGQEARQRGI LDAVKEMIPL DLLLAVPVPG VNIEEHLQLR QKEKRQRVIR RHRLEDGRGP 850 860 870 880 890 900 LVFAGPIFMN HREQALARLR SHPAQLKHKR DKHKDGSGDR GEKDASKITT YPPGSVRFDC 910 920 930 940 950 960 ELRAVQVSCG FHHSVVLMEN GDVYTFGYGQ HGQLGHGDVN SRGCPTLVQA LPGPSTQVTA 970 980 990 1000 1010 1020 CSNHTAVLLM DGQVFTFGSF SKGQLGRPIL DIPYWNAKPA PMPNIGSKYG RKATWIGASG 1030 1040 1050 1060 1070 1080 DQTFLRIDEA LINSHVLATS EIFASKHIIG LVPASISEPP PFKCLLINKV DGSCKTFNDS 1090 1100 1110 1120 1130 1140 EQEDLQGFGV CLDPVYDVLW RFRPSTRELW CYNAVVADAR LPSATDMQSR CSILSPELAL 1150 1160 1170 1180 1190 1200 PTGSRALTTR SHAALHILGC LDTLAAMQDL KMGIASTEEE TQAVMKVYSK EDYSVVNRFE 1210 1220 1230 1240 1250 1260 SHGGGWGYSA HSVEAIRFSA DTDILLGGLG LFGGRGEYTA KIKLFELGPD GGDHETDGDL 1270 1280 1290 1300 1310 1320 LAETDVLAYD CAAREKYAMM FDEPVLLQAG WWYVAWARVS GPSSDCGSHG QASITTDDGV 1330 1340 1350 1360 1370 1380 IFQFKSSKKS NNGTDVNAGQ IPQLLYRLPT SDGSTSKGKQ QTSEPVHILK RSFARTVSVE 1390 1400 1410 1420 1430 1440 CFESLLSILH WSWTTLVLGV EELRGLKGFQ FTATLLDLER LRFVGTCCLR LLRVYTCEIY 1450 1460 1470 1480 1490 1500 PVSATGKAVV EETSKLAECI GKTRTLLRKI LSEGVDHCMV KLDNDPQGYL SQPLRLLEAV 1510 1520 1530 1540 1550 1560 LQECHNTFTA CFHSFYPTPA LQWACLCDLL NCLDQEANFK TSSSRLLAAV MSALCHTSVK 1570 1580 1590 1600 1610 1620 LTSLFPIAYD GEVLLRSIVK QVSTENDSTL VHRFPLLVGH MEKLSQSEEN ISGMTSFREV 1630 1640 1650 1660 1670 1680 LEKMLVIVVL PVRNSLRRES ELFSSHLVSN TCGLLASIVS ELTASALGSE VDGLNSLHSV 1690 1700 1710 1720 1730 1740 KASANRFTKT SQGRSWNTGN GSPDAICFAV DKPGIVVVGF AVYGGGGIHE YELEVLVDDS 1750 1760 1770 1780 1790 1800 EHAGDSTHSH RWTSLELVKG TYTTDDSPSD IAEIRLDKVV PLKENVKYAV RLSNYGSRTA 1810 1820 1830 1840 1850 1860 NGDGGMTTVQ CPDGVTFTFS TCSLSSNGTN QTRGQIPQIL YYRSEFDGDL QSQLLSKANE 1870 1880 1890 1900 1910 1920 EDKNCSRALS VVSTVVRAAK DLLHRALAVD ADDIPELLSS SSLFSMLLPL IIAYIGPVAA 1930 1940 1950 1960 1970 1980 AIPKVAVEVF GLVQQLLPSV AILNQKYASP AFNPNQSTDS TTGNQPEQGL SACTTSNHYA 1990 2000 2010 2020 2030 2040 VIESEHPYKP ACVMHYKVTF PECVRWMTIE FDPQCGTAQS EDVIRLLIPV RTIQNSGYGA 2050 2060 2070 2080 2090 2100 KLTSVHENLN SWVELKKYSG SSGWPTMVLV LPGNEALFSL ETASDYVKDD KASFYGFKCF 2110 2120 2130 2140 2150 2160 AIGYEFSPGP DEGVIQLEKE LANLGGVCAA ALMKKDLALP VGNELEEDLE ILEEAALQVC 2170 2180 2190 2200 2210 2220 KTHSGILGKG LALSHSPTIL EALEGNLPLQ IQSNEQSFLD DFIACVPGSS GGRLARWLQP 2230 2240 2250 2260 2270 2280 DSYADPQKTS LILNKDDIRC GWPTTITVQT KDQYGDVVHV PNMKVEVKAV PVSQKKTSLQ 2290 2300 2310 2320 2330 2340 QDQGKKCQRI PGSPSAAASS ADMTFGGLAS PKLDVSYEPM IVKEARYIAI TMMKVYENYS 2350 2360 2370 2380 2390 2400 FEELRFASPT PKRPSENMLI RVNNDGTYCA NWTPGAIGLY TVHVTIDGIE IDAGLEVKVK 2410 2420 2430 2440 2450 2460 DPPKGMIPPG TQLVKPKADP QPNKIRKFVA KDSAGLRIRS HPSLQSEQIG IVRVNGTITF 2470 2480 2490 2500 2510 2520 IDEIHNDDGV WLRLNEETIK KYVPNMNGYT EAWCLSFNQH LGKSLLVPVD NIFNASQGVR 2530 2540 2550 2560 2570 2580 DLDVFSWTSK AFFPQEPKTN TDDFFKDMNS CGPQEATMQE RDHPFLRGGP GMYKVVKTGP 2590 2600 2610 2620 2630 2640 SGHNIRSCPN LRGIPIGMLV LGNKVKAVGE VTNSEGAWVQ LDKNSMVEFC ESDEGEAWSL 2650 2660 2670 2680 2690 2700 ARDRGGNQYL RHEDEQVLLD QNSQPPPPSP FSVQAFNKGA SCSAQGFDYG LGNNKGDQLS 2710 2720 2730 2740 2750 2760 AILNSIQSRP NLPAPSIFDQ AAKPPSSLVH SPFVFGQPLS FQQRQLQSDR GTISTSSRPV 2770 2780 2790 2800 2810 2820 STSGKSELPS KHSRSVKPDG HVSRTPADQK KPRGTEGLSA SESLMLKSDA AKLRSDSHSR 2830 2840 2850 2860 2870 2880 SLSPNHNTLQ TLKSDGRTSS GFRAESPGPG SRSSSPKPKP LPTPRSSPSG ASSPRSSSPQ 2890 2900 2910 2920 2930 2940 DKNLPQKSTA PAKTKLDPPR ERSKSDSYTL DPDTLRKKKM PLTEPLRGRS TSPKPKPVPK 2950 2960 2970 2980 2990 3000 DPKDSPGSEN RAPSPHVVQE NLHSEVVEVC TSSTLKTNGV TDSTCDDSGD LKSVDEGSNK 3010 3020 3030 3040 3050 3060 VHFSIGKAPL KDEQEMRASP KISRKCANRH TRPKKEKSNF LFKGDGTKSL EPAKQAMSPS 3070 3080 3090 3100 3110 3120 VAECARAVFA SFLWHEGIVH DAMACSSFLK FNPDLSKEHA PIRSSLNSQP PTEEKEIKLK 3130 3140 3150 3160 3170 3180 NRHSLEISSA LNMFNIAPHG PDISKMGSIN KNKVLSMLKE PPLHEKCEDG KSEATFEMSM 3190 3200 3210 3220 3230 3240 HHTMKSKSPL PLTLQHLVAF WEDISLATIK AASQNMIFPS PGSCAVLKKK ECEKENKKTK 3250 3260 3270 3280 3290 3300 KEKKKKEKTE IRPRGNLFGE MAQLAVGGPE KDTICELCGE SHPYPVTYHM RQAHPGCGRY 3310 3320 3330 3340 3350 3360 AGGQGYNSIG HFCGGWAGNC GDGGMGGSTW YLVCDRCREK YLREKQAAAR EKVKQSRRKP 3370 3380 3390 3400 3410 3420 MQVKTPRALP TMEAHQVIKA NALFLLSLSS AAEPSILCYH PAKPFQSQLP IVKEGVSEDL 3430 3440 3450 3460 3470 3480 PVKMPCLYLQ TLARHHHENF VGYQDDNLFQ DEMRYLRSTS VPAPYISVTP DASPNVFEEP 3490 3500 3510 3520 3530 3540 ESNMKSMPPS LETSPITDTD LAKRTVFQRS YSVVASEYDK QHSILPARVK AIPRRRVNSG 3550 3560 3570 3580 3590 3600 DTVGSSLLRH PSPELSRLIS AHSSLSKGER NFQWPVLAFV IQHHDLEGLE IAMKQALRKS 3610 3620 3630 3640 3650 3660 ACRVFAMEAF NWLLCNVIQT TSLHDILWHF VAALTPSPVE AEEDEDGDNK SNKENAEQEK 3670 3680 3690 3700 3710 3720 DTRVCEHPLS DIVIAGEAAH PLPHTFHRLL QTISDLMMSL PSGSSLQQMA LRCWSLKFKQ 3730 3740 3750 3760 3770 3780 SDHQFLHQSN VFHHINNILS KSDDGDSEES FSISVQSGFE AMSQELCIVM CLKDLTSIVD 3790 3800 3810 3820 3830 3840 IKTSSRPAMI GSLTDGSTET FWESGDEDKN KTKNITINCV KGINARYVSV HVDNSRDLGN 3850 3860 3870 3880 3890 3900 KVTSMTFLTG KAVEELCRIK QVDLDSRHIG WVTSELPGGD NQIIKIELKG PENTLRVRQV 3910 3920 3930 3940 3950 3960 KVLGWKDGES TKIAGQISAS VAQQRSCEAE TLRVFRLITS QVFGKLISGD AEPTPEQEEK 3970 3980 3990 4000 4010 4020 ALLSSPEGEE KATSDADLKE HMVGIIFSRS KLTNLQKQVC AHIVQAIRME ATRVREEWEH 4030 4040 4050 4060 4070 4080 AISSKENANS QPSDEDASSD AYCFELLSMV LALSGSNVGR QYLAQQLTLL QDLFSLLHTA 4090 4100 4110 4120 4130 4140 SPRVQRQVTS LLRRVLPEVT PNRLASIIGV KSLPPADISD IIHSTEKGDW NKLGILDMFL 4150 4160 4170 4180 4190 4200 GCIAKALTVQ LKAKGTTITG TAGTTVGKGV TTVTLPMIFN SSYLRRGESH WWMKGSTPTQ 4210 4220 4230 4240 4250 4260 ISEIIIRLIK DMAAGHLSEA WSRVTKNAIA ETIIALTKME EEFRSPVRCI ATTRLWLALA 4270 4280 4290 4300 4310 4320 SLCVLDQDHV DRLSSGRWMG KDGQQKQMPM CDNHDDGETA AIILCNICGN LCTDCDRFLH 4330 4340 4350 4360 4370 4380 LHRRTKTHQR QVFKEEEEAI KVDLHEGCGR TKLFWLMALA DSKTMKAMVE FREHTGKPTT 4390 4400 4410 4420 4430 4440 SSSEACRFCG SRSGTELSAV GSVCSDADCQ EYAKIACSKT HPCGHPCGGV RNEEHCLPCL 4450 4460 4470 4480 4490 4500 HGCDKSATTL KQDADDMCMI CFTEALSAAP AIQLDCSHVF HLQCCRRVLE NRWLGPRITF 4510 4520 4530 4540 4550 4560 GFISCPICKN KINHIVLKDL LDPIKELYED VRRKALMRLE YEGLHKSEAI TTPGVRFYND 4570 4580 4590 4600 4610 4620 AAGYAMNRYA YYVCYKCRKA YFGGEARCDA EAGQEDDYDP RELICGACSD VSRAQMCPKH 4630 4640 4650 4660 4670 4680 GTDFLEYKCR YCCSVAVFFC FGTTHFCNAC HDDFQRMTSI PKEELPHCPA GPKGKQLEGT 4690 4700 ECPLHVVHPP TGEEFALGCG VCRNAHTF « Hide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for a conserved function in synapse formation reveals Phr1 as a candidate gene for respiratory failure in newborn mice." Burgess R.W., Peterson K.A., Johnson M.J., Roix J.J., Welsh I.C., O'Brien T.P. Mol. Cell. Biol. 24:1096-1105(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE. Strain: C57BL/6J. Tissue: Embryo.
[2]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2327-2352 AND 2866-2875, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3250-4711 (ISOFORM 2). Strain: C57BL/6. Tissue: Brain.
[4]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1583 AND THR-3954, MASS SPECTROMETRY. Tissue: Brain cortex.
[5]	"Fbxo45 forms a novel ubiquitin ligase complex and is required for neuronal development." Saiga T., Fukuda T., Matsumoto M., Tada H., Okano H.J., Okano H., Nakayama K.I. Mol. Cell. Biol. 29:3529-3543(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FBXO45.
[6]	"Structures of PHR domains from Mus musculus Phr1 (Mycbp2) explain the loss-of-function mutation (Gly1092-->Glu) of the C. elegans ortholog RPM-1." Sampathkumar P., Ozyurt S.A., Miller S.A., Bain K.T., Rutter M.E., Gheyi T., Abrams B., Wang Y., Atwell S., Luz J.G., Thompson D.A., Wasserman S.R., Emtage J.S., Park E.C., Rongo C., Jin Y., Klemke R.L., Sauder J.M., Burley S.K. J. Mol. Biol. 397:883-892(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.32 ANGSTROMS) OF 1191-1352, X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 1685-1845, DOMAIN PHR, DISULFIDE BOND.
+	Additional computationally mapped references.

EMBL
GenBank
DDBJ

AY325887 mRNA. Translation: AAP88591.1.
BC059257 mRNA. Translation: AAH59257.1.

IPI

IPI00380409.
IPI00608114.

RefSeq

NP_997098.2. NM_207215.2.

UniGene

Mm.6478.

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3GBW	X-ray	1.32	A	1191-1352	[»]
3HWJ	X-ray	2.25	A/B	1685-1845	[»]

ProteinModelPortal

Q7TPH6.

SMR

Q7TPH6. Positions 496-677, 876-989, 1189-1349, 1685-1844.

ModBase

Search...

IntAct

Q7TPH6. 1 interaction.

PhosphoSite

Q7TPH6.

PaxDb

Q7TPH6.

PRIDE

Q7TPH6.

DNASU

105689.

StructuralBiologyKnowledgebase

Search...

GeneID

105689.

KEGG

mmu:105689.

UCSC

uc007uwl.1. mouse.

CTD

23077.

MGI

MGI:2179432. Mycbp2.

eggNOG

NOG240798.

HOGENOM

HOG000112908.

HOVERGEN

HBG053153.

InParanoid

Q7TPH6.

KO

K10693.

OrthoDB

EOG4VQ9NB.

UniPathway

UPA00143.

CleanEx

MM_MYCBP2.
MM_PAM.

Genevestigator

Q7TPH6.

GermOnline

ENSMUSG00000033004. Mus musculus.

Gene3D

2.130.10.30. 2 hits.
2.60.40.10. 2 hits.
3.30.40.10. 1 hit.

InterPro

IPR004939. APC_su10/DOC_dom.
IPR017868. Filamin/ABP280_repeat-like.
IPR008979. Galactose-bd-like.
IPR027047. Highwire/Pam/Rpm-1.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR012983. PHR.
IPR009091. RCC1/BLIP-II.
IPR000408. Reg_chr_condens.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

PANTHER

PTHR12846. PTHR12846. 1 hit.

Pfam

PF00630. Filamin. 1 hit.
PF08005. PHR. 2 hits.
PF00415. RCC1. 1 hit.
PF13639. zf-RING_2. 1 hit.
[Graphical view]

PRINTS

PR00633. RCCNDNSATION.

SMART

SM00184. RING. 1 hit.
[Graphical view]

SUPFAM

SSF49785. Gal_bind_like. 1 hit.
SSF81296. Ig_E-set. 1 hit.
SSF50985. RCC1/BLIP-II. 1 hit.

PROSITE

PS51284. DOC. 1 hit.
PS50194. FILAMIN_REPEAT. 1 hit.
PS00625. RCC1_1. False negative.
PS00626. RCC1_2. 1 hit.
PS50012. RCC1_3. 3 hits.
PS50119. ZF_BBOX. False negative.
PS00518. ZF_RING_1. False negative.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

ProtoNet

Search...

ChiTaRS

MYCBP2. mouse.

EvolutionaryTrace

Q7TPH6.

NextBio

357830.

SOURCE

Search...

Entry name

MYCB2_MOUSE

Accession

Primary (citable) accession number: Q7TPH6
Secondary accession number(s): Q6PCM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	May 1, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q7TPH6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7TPH6-2)

The sequence of this isoform differs from the canonical sequence as follows:
3972-3974: Missing.