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Protein

Roundabout homolog 2

Gene

Robo2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.1 Publication

GO - Molecular functioni

GO - Biological processi

  • axon guidance Source: MGI
  • axon midline choice point recognition Source: UniProtKB
  • brain development Source: UniProtKB
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: UniProtKB
  • metanephros development Source: MGI
  • negative regulation of negative chemotaxis Source: MGI
  • olfactory bulb interneuron development Source: UniProtKB
  • positive regulation of axonogenesis Source: UniProtKB
  • retinal ganglion cell axon guidance Source: UniProtKB
  • Roundabout signaling pathway Source: InterPro
  • ureteric bud development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Chemotaxis, Differentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-MMU-428542. Regulation of Commissural axon pathfinding by Slit and Robo.

Names & Taxonomyi

Protein namesi
Recommended name:
Roundabout homolog 2
Gene namesi
Name:Robo2
Synonyms:Kiaa1568
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1890110. Robo2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 863ExtracellularSequence analysisAdd BLAST842
Transmembranei864 – 884HelicalSequence analysisAdd BLAST21
Topological domaini885 – 1470CytoplasmicSequence analysisAdd BLAST586

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show defects in commissural axon guidance in spinal chord including an altered lateral and ventral funiculi projection. The phenotype resembles that of a SLIT1;SLIT2;SLIT3 triple mutant.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000003103722 – 1470Roundabout homolog 2Add BLAST1449

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52 ↔ 110PROSITE-ProRule annotation
Glycosylationi123N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi154 ↔ 203PROSITE-ProRule annotation
Disulfide bondi246 ↔ 293PROSITE-ProRule annotation
Disulfide bondi339 ↔ 395PROSITE-ProRule annotation
Glycosylationi430N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi443 ↔ 492PROSITE-ProRule annotation
Glycosylationi756N-linked (GlcNAc...)Sequence analysis1
Glycosylationi786N-linked (GlcNAc...)Sequence analysis1
Glycosylationi793N-linked (GlcNAc...)Sequence analysis1
Glycosylationi849N-linked (GlcNAc...)Sequence analysis1
Modified residuei1157PhosphothreonineCombined sources1
Modified residuei1159PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ7TPD3.
PRIDEiQ7TPD3.

PTM databases

iPTMnetiQ7TPD3.
PhosphoSitePlusiQ7TPD3.

Expressioni

Tissue specificityi

Expressed in embryonal spinal chord.1 Publication

Developmental stagei

Expressed at E11.5 in spinal chord.1 Publication

Gene expression databases

CleanExiMM_ROBO2.

Interactioni

Subunit structurei

Interacts with SLIT2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ7TPD3. 1 interactor.
STRINGi10090.ENSMUSP00000112776.

Structurei

3D structure databases

ProteinModelPortaliQ7TPD3.
SMRiQ7TPD3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 127Ig-like C2-type 1Add BLAST97
Domaini133 – 220Ig-like C2-type 2Add BLAST88
Domaini225 – 309Ig-like C2-type 3Add BLAST85
Domaini318 – 413Ig-like C2-type 4Add BLAST96
Domaini422 – 508Ig-like C2-type 5Add BLAST87
Domaini528 – 622Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST95
Domaini641 – 739Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST99
Domaini743 – 840Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST98

Sequence similaritiesi

Belongs to the immunoglobulin superfamily. ROBO family.Curated
Contains 3 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4222. Eukaryota.
ENOG410XPQS. LUCA.
HOGENOMiHOG000010267.
HOVERGENiHBG073476.
InParanoidiQ7TPD3.

Family and domain databases

CDDicd00063. FN3. 3 hits.
Gene3Di2.60.40.10. 9 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR032985. Robo2.
[Graphical view]
PANTHERiPTHR10489:SF27. PTHR10489:SF27. 2 hits.
PfamiPF00041. fn3. 3 hits.
PF07679. I-set. 2 hits.
[Graphical view]
SMARTiSM00060. FN3. 3 hits.
SM00409. IG. 5 hits.
SM00408. IGc2. 5 hits.
SM00406. IGv. 3 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
SSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 3 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TPD3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNPLMFTLLL LFGFLCIQID GSRLRQEDFP PRIVEHPSDV IVSKGEPTTL
60 70 80 90 100
NCKAEGRPTP TIEWYKDGER VETDKDDPRS HRMLLPSGSL FFLRIVHGRR
110 120 130 140 150
SKPDEGSYVC VARNYLGEAV SRNASLEVAL LRDDFRQNPT DVVVAAGEPA
160 170 180 190 200
ILECQPPRGH PEPTIYWKKD KVRIDDKEER ISIRGGKLMI SNTRKSDAGM
210 220 230 240 250
YTCVGTNMVG ERDSDPAELT VFERPTFLRR PINQVVLEEE AVEFRCQVQG
260 270 280 290 300
DPQPTVRWKK DDADLPRGRY DIKDDYTLRI KKAMSTDEGT YVCIAENRVG
310 320 330 340 350
KVEASATLTV RVRPVAPPQF VVRPRDQIVA QGRTVTFPCE TKGNPQPAVF
360 370 380 390 400
WQKEGSQNLL FPNQPQQPNS RCSVSPTGDL TITNIQRSDA GYYICQALTV
410 420 430 440 450
AGSILAKAQL EVTDVLTDRP PPIILQGPIN QTLAVDGTAL LKCKATGEPL
460 470 480 490 500
PVISWLKEGF TFLGRDPRAT IQDQGTLQIK NLRISDTGTY TCVATSSSGE
510 520 530 540 550
TSWSAVLDVT ESGATISKNY DMNDLPGPPS KPQVTDVSKN SVTLSWQPGT
560 570 580 590 600
PGVLPASAYI IEAFSQSVSN SWQTVANHVK TTLYTVRGLR PNTIYLFMVR
610 620 630 640 650
AINPQGLSDP SPMSDPVRTQ DISPPAQGVD HRQVQKELGD VVVRLHNPVV
660 670 680 690 700
LTPTTVQVTW TVDRQPQFIQ GYRVMYRQTS GLQASTVWQN LDAKVPTERS
710 720 730 740 750
AVLVNLKKGV TYEIKVRPYF NEFQGMDSES KTVRTTEEAP SAPPQSVTVL
760 770 780 790 800
TVGSHNSTSI SVSWDPPPAD HQNGIIQEYK IWCLGNETRF HINKTVDAAI
810 820 830 840 850
RSVVIGGLFP GIQYRVEVAA STSAGVGVKS EPQPIIIGGR NEVVITENNN
860 870 880 890 900
SITEQITDVV KQPAFIAGIG GACWVILMGF SIWLYWRRKK RKGLSNYAVT
910 920 930 940 950
FQRGDGGLMS NGSRPGLLNA GDPNYPWLAD SWPATSLPVN NSNSGPNEIG
960 970 980 990 1000
NFGRGDVLPP VPGQGDKTAT MLSDGAIYSS IDFTTKTTYN SSSQITQATP
1010 1020 1030 1040 1050
YATTQILHSN SIHELAVDLP DPQWKSSVQQ KTDLMGFGYS LPDQNKGNNG
1060 1070 1080 1090 1100
GKGGKKKKTK NSSKAQKNNG STWANVPLPP PPVQPLPGTE LGHYAAEQEN
1110 1120 1130 1140 1150
GYDSDSWCPP LPVQTYLHQG MEDELEEDED RVPTPPVRGV ASSPAISFGQ
1160 1170 1180 1190 1200
QSTATLTPSP REEMQPMLQA HLDELTRAYQ FDIAKQTWHI QSNTPPPQPP
1210 1220 1230 1240 1250
APPLGYVSGA LISDLETDVP DEDADDEEEP LEIPRPLRAL DQTPGSSMDN
1260 1270 1280 1290 1300
LDSSVTGKAF SSSQRQRPTS PFSTDSNTSA AQNQSQRPRP TKKHKGGRMD
1310 1320 1330 1340 1350
PQPVLPHRRE GMPDDLPPPP DPPPGQGLRQ QIGLSQHSGN VENSTERKGS
1360 1370 1380 1390 1400
SLERQQAANL EDTKSSLDCP AKTVLEWQRQ TQDWINSTER QEETRKAPHK
1410 1420 1430 1440 1450
QGVGSEESLV PYSKPSFPSP GGHSSSGTSS SKGSTGPRKA DVLRGSHQRN
1460 1470
ANDLLDIGYV GSNSQGQFTE
Length:1,470
Mass (Da):161,190
Last modified:June 21, 2004 - v2
Checksum:i3C61DE40DA39109E
GO
Isoform 2 (identifier: Q7TPD3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     807-946: GLFPGIQYRV...LPVNNSNSGP → ASTSRPPEWNYSGI
     947-1470: Missing.

Note: No experimental confirmation available.
Show »
Length:820
Mass (Da):90,952
Checksum:iECB1F6D26983065E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010648807 – 946GLFPG…SNSGP → ASTSRPPEWNYSGI in isoform 2. 1 PublicationAdd BLAST140
Alternative sequenceiVSP_010649947 – 1470Missing in isoform 2. 1 PublicationAdd BLAST524

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055333 mRNA. Translation: AAH55333.1.
AK129396 mRNA. Translation: BAC98206.1.
UniGeneiMm.171736.

Genome annotation databases

UCSCiuc007zrf.1. mouse. [Q7TPD3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055333 mRNA. Translation: AAH55333.1.
AK129396 mRNA. Translation: BAC98206.1.
UniGeneiMm.171736.

3D structure databases

ProteinModelPortaliQ7TPD3.
SMRiQ7TPD3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7TPD3. 1 interactor.
STRINGi10090.ENSMUSP00000112776.

PTM databases

iPTMnetiQ7TPD3.
PhosphoSitePlusiQ7TPD3.

Proteomic databases

PaxDbiQ7TPD3.
PRIDEiQ7TPD3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc007zrf.1. mouse. [Q7TPD3-1]

Organism-specific databases

MGIiMGI:1890110. Robo2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG4222. Eukaryota.
ENOG410XPQS. LUCA.
HOGENOMiHOG000010267.
HOVERGENiHBG073476.
InParanoidiQ7TPD3.

Enzyme and pathway databases

ReactomeiR-MMU-428542. Regulation of Commissural axon pathfinding by Slit and Robo.

Miscellaneous databases

ChiTaRSiRobo2. mouse.
PROiQ7TPD3.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ROBO2.

Family and domain databases

CDDicd00063. FN3. 3 hits.
Gene3Di2.60.40.10. 9 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR032985. Robo2.
[Graphical view]
PANTHERiPTHR10489:SF27. PTHR10489:SF27. 2 hits.
PfamiPF00041. fn3. 3 hits.
PF07679. I-set. 2 hits.
[Graphical view]
SMARTiSM00060. FN3. 3 hits.
SM00409. IG. 5 hits.
SM00408. IGc2. 5 hits.
SM00406. IGv. 3 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
SSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 3 hits.
PS50835. IG_LIKE. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiROBO2_MOUSE
AccessioniPrimary (citable) accession number: Q7TPD3
Secondary accession number(s): Q8BJ59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 21, 2004
Last modified: November 30, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.