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Protein

STE20-related kinase adapter protein alpha

Gene

Strada

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • activation of protein kinase activity Source: UniProtKB
  • cell cycle Source: UniProtKB-KW
  • protein export from nucleus Source: UniProtKB
  • protein heterooligomerization Source: RGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Names & Taxonomyi

Protein namesi
Recommended name:
STE20-related kinase adapter protein alpha
Short name:
STRAD alpha
Alternative name(s):
STE20-related adapter protein
Gene namesi
Name:Strada
Synonyms:Lyk5Imported, Strad
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727905. Strada.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393STE20-related kinase adapter protein alphaPRO_0000260039Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei381 – 3811Phosphothreonine; by LKB1By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7TNZ6.
PRIDEiQ7TNZ6.

PTM databases

iPTMnetiQ7TNZ6.
PhosphoSiteiQ7TNZ6.

Expressioni

Tissue specificityi

Expressed in liver.1 Publication

Interactioni

Subunit structurei

Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity.By similarity

Protein-protein interaction databases

IntActiQ7TNZ6. 2 interactions.
STRINGi10116.ENSRNOP00000011894.

Structurei

3D structure databases

ProteinModelPortaliQ7TNZ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 341310Protein kinasePROSITE-ProRule annotationAdd
BLAST

Domaini

The protein kinase domain is predicted to be catalytically inactive.Sequence analysis

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0582. Eukaryota.
ENOG410XSWS. LUCA.
HOGENOMiHOG000237355.
HOVERGENiHBG055069.
InParanoidiQ7TNZ6.
KOiK08271.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q7TNZ6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFLANEASS ESIASFSKPE IMSSFLPEGG CYELLSVIGK GFEDLMTVNS
60 70 80 90 100
RYKPTGEYVT VRRINLEACS NEMVTFLQGE LHVSKLFSHP NIVPYRATFI
110 120 130 140 150
ADNELWAVTS FMAYGSAKDL IGTHFMDGMS ELAIAYILQG VLKALDYIHH
160 170 180 190 200
MGYVHRSVKA SHILISTDGK VYLSGLRSNL SMISHGQRQR AVHDFPKYSI
210 220 230 240 250
KVLPWLSPEV LQQNLQGYDA KSDIYSVGIT ACELANGHVP FKDMPATQML
260 270 280 290 300
LEKLNGTVPC LLDTSTIPAE ELTMSPSRSI ANPGLNDSLA AGSLRPANGD
310 320 330 340 350
SPSHPYHRTF SPHFHNFVEQ CLQRNPDARP NASTLLNHSF FKQIKRRASE
360 370 380 390
ALPELLRPVT PITSFEGSQS QDHSGILGLV TNLEDLEVDD WEF
Length:393
Mass (Da):43,553
Last modified:October 1, 2003 - v1
Checksum:iB92AB012E2A8AD34
GO
Isoform 21 Publication (identifier: Q7TNZ6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:372
Mass (Da):41,326
Checksum:i1B990C714C58DDCB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501S → LA in AAH81911 (PubMed:15489334).Curated
Sequence conflicti133 – 1331A → V in AAH81911 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2121Missing in isoform 2. 1 PublicationVSP_052216Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY327409 mRNA. Translation: AAP92801.1.
BC081911 mRNA. Translation: AAH81911.1.
RefSeqiNP_877972.1. NM_182820.1. [Q7TNZ6-1]
UniGeneiRn.11563.

Genome annotation databases

GeneIDi303605.
KEGGirno:303605.
UCSCiRGD:727905. rat. [Q7TNZ6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY327409 mRNA. Translation: AAP92801.1.
BC081911 mRNA. Translation: AAH81911.1.
RefSeqiNP_877972.1. NM_182820.1. [Q7TNZ6-1]
UniGeneiRn.11563.

3D structure databases

ProteinModelPortaliQ7TNZ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7TNZ6. 2 interactions.
STRINGi10116.ENSRNOP00000011894.

PTM databases

iPTMnetiQ7TNZ6.
PhosphoSiteiQ7TNZ6.

Proteomic databases

PaxDbiQ7TNZ6.
PRIDEiQ7TNZ6.

Protocols and materials databases

DNASUi303605.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi303605.
KEGGirno:303605.
UCSCiRGD:727905. rat. [Q7TNZ6-1]

Organism-specific databases

CTDi92335.
RGDi727905. Strada.

Phylogenomic databases

eggNOGiKOG0582. Eukaryota.
ENOG410XSWS. LUCA.
HOGENOMiHOG000237355.
HOVERGENiHBG055069.
InParanoidiQ7TNZ6.
KOiK08271.

Miscellaneous databases

PROiQ7TNZ6.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of human, mouse and rat Lyk5 gene."
    Shan Y.X., Yu L.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-DawleyImported.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: TestisImported.
  3. "Complexes between the LKB1 tumor suppressor, STRAD alpha/beta and MO25 alpha/beta are upstream kinases in the AMP-activated protein kinase cascade."
    Hawley S.A., Boudeau J., Reid J.L., Mustard K.J., Udd L., Makela T.P., Alessi D.R., Hardie D.G.
    J. Biol. 2:28.1-28.16(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INTERACTION WITH CAB39 AND STK11.

Entry informationi

Entry nameiSTRAA_RAT
AccessioniPrimary (citable) accession number: Q7TNZ6
Secondary accession number(s): Q66HD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 1, 2003
Last modified: June 8, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.