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Protein

Golgi resident protein GCP60

Gene

Acbd3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi resident protein GCP60
Alternative name(s):
Acyl-CoA-binding domain-containing protein 3
DMT1-associated protein
Short name:
DAP
Golgi complex-associated protein 1
Short name:
GOCAP1
Golgi phosphoprotein 1
Short name:
GOLPH1
Gene namesi
Name:Acbd3
Synonyms:Gcp60
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727851. Acbd3.

Subcellular locationi

GO - Cellular componenti

  • Golgi membrane Source: UniProtKB
  • integral component of membrane Source: InterPro
  • mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 526525Golgi resident protein GCP60PRO_0000436451Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei13 – 131PhosphoserineBy similarity
Modified residuei18 – 181PhosphothreonineBy similarity
Modified residuei20 – 201PhosphoserineBy similarity
Modified residuei40 – 401PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ7TNY6.
PRIDEiQ7TNY6.

PTM databases

iPTMnetiQ7TNY6.
PhosphoSiteiQ7TNY6.

Interactioni

Subunit structurei

Interacts with the C-terminal cytoplasmic domain of giantin/GOLGB1 (By similarity). Interacts with PBR and PKA regulatory subunit RI-alpha. Does not interact with PKA regulatory subunit RI-beta nor PKA regulatory subunit RII-alpha (By similarity). Interacts with PI4KB, TBC1D22A AND TBC1D22B; interactions with PI4KB and with TBC1D22A and TBC1D22B are mutually exclusive. Interacts with C10ORF76 and RAB11B (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-4576509.
STRINGi10116.ENSRNOP00000004293.

Structurei

3D structure databases

ProteinModelPortaliQ7TNY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 17192ACBPROSITE-ProRule annotationAdd
BLAST
Domaini381 – 524144GOLDPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili169 – 25486Sequence analysisAdd
BLAST
Coiled coili450 – 47122Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi179 – 1846Poly-Glu
Compositional biasi186 – 23550Arg-richAdd
BLAST
Compositional biasi238 – 30568Gln-richAdd
BLAST

Domaini

The GOLD domain is essential for giantin binding.By similarity

Sequence similaritiesi

Contains 1 ACB (acyl-CoA-binding) domain.PROSITE-ProRule annotation
Contains 1 GOLD domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3878. Eukaryota.
ENOG410XS5V. LUCA.
HOGENOMiHOG000247039.
HOVERGENiHBG051712.
InParanoidiQ7TNY6.
PhylomeDBiQ7TNY6.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
InterProiIPR022408. Acyl-CoA-binding_prot_CS.
IPR000582. Acyl-CoA-binding_protein.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR009038. GOLD_dom.
[Graphical view]
PfamiPF00887. ACBP. 1 hit.
PF13897. GOLD_2. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 2 hits.
SSF47027. SSF47027. 1 hit.
PROSITEiPS00880. ACB_1. 1 hit.
PS51228. ACB_2. 1 hit.
PS50866. GOLD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7TNY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQLNVEQL EVSLDGLTLS PDSEERPGAE GAPLQTPPSS PPRDGLGSGT
60 70 80 90 100
AGQQREPGEA AAEGAAEEAR RMEQHWGFGL EELYGLALRF YKIKDGKAFH
110 120 130 140 150
PTYEEKLKFV ALHKQVLLGP YNPDTSPEVG FFDVLGNDRR REWAALGNMS
160 170 180 190 200
KEDAMVEFVK LLNKCCPLLS AYVASHRIEK EEEEKRRKAE EERRQREEEE
210 220 230 240 250
RERLQKEEEK RKREEEDRLR REEEERRRIE EERLRLEQQK QQIMAALNSQ
260 270 280 290 300
TAVQFQQYAA QQYPGNYEQQ QILIRQLQEQ HYQQYMQQLY QVQLAQQQAA
310 320 330 340 350
LQKQQEVVVA GASLPASTKV NTAGASDPLP VNGQAKTHTE NPEKVLEPEA
360 370 380 390 400
AEEALENGPK DSLPVIAAPS MWTRPQIKDF KEKIRQDADS VITVRRGEVV
410 420 430 440 450
TVRVPTHEEG SYLFWEFATD SYDIGFGVYF EWTDSPNAAV SVHVSESSDD
460 470 480 490 500
EEEEEEENVT CEEKAKKNVN KPLLDEIVPV YRRDCHEEVY AGSHQYPGRG
510 520
VYLLKFDNSY SLWRSKSVYY RVYYTR
Length:526
Mass (Da):60,479
Last modified:January 23, 2007 - v3
Checksum:iFB1147D95DA48B45
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY336075 mRNA. Translation: AAP94639.1.
RefSeqiNP_878263.1. NM_182843.1.
UniGeneiRn.57036.

Genome annotation databases

GeneIDi289312.
KEGGirno:289312.
UCSCiRGD:727851. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY336075 mRNA. Translation: AAP94639.1.
RefSeqiNP_878263.1. NM_182843.1.
UniGeneiRn.57036.

3D structure databases

ProteinModelPortaliQ7TNY6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4576509.
STRINGi10116.ENSRNOP00000004293.

PTM databases

iPTMnetiQ7TNY6.
PhosphoSiteiQ7TNY6.

Proteomic databases

PaxDbiQ7TNY6.
PRIDEiQ7TNY6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi289312.
KEGGirno:289312.
UCSCiRGD:727851. rat.

Organism-specific databases

CTDi64746.
RGDi727851. Acbd3.

Phylogenomic databases

eggNOGiKOG3878. Eukaryota.
ENOG410XS5V. LUCA.
HOGENOMiHOG000247039.
HOVERGENiHBG051712.
InParanoidiQ7TNY6.
PhylomeDBiQ7TNY6.

Miscellaneous databases

PROiQ7TNY6.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
InterProiIPR022408. Acyl-CoA-binding_prot_CS.
IPR000582. Acyl-CoA-binding_protein.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR009038. GOLD_dom.
[Graphical view]
PfamiPF00887. ACBP. 1 hit.
PF13897. GOLD_2. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 2 hits.
SSF47027. SSF47027. 1 hit.
PROSITEiPS00880. ACB_1. 1 hit.
PS51228. ACB_2. 1 hit.
PS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCP60_RAT
AccessioniPrimary (citable) accession number: Q7TNY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.