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Protein

BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2

Gene

Tnfaip1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD2) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis (By similarity). May enhance the PCNA-dependent DNA polymerase delta activity.By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: UniProtKB
  • cell migration Source: UniProtKB
  • DNA repair Source: UniProtKB
  • DNA replication Source: UniProtKB
  • embryo development Source: UniProtKB
  • immune response Source: UniProtKB
  • negative regulation of Rho protein signal transduction Source: UniProtKB
  • positive regulation of DNA replication Source: RGD
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  • protein heterooligomerization Source: UniProtKB
  • protein homooligomerization Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • stress fiber assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
Alternative name(s):
BTB/POZ domain-containing protein TNFAIP1
Tumor necrosis factor-induced protein 1
Gene namesi
Name:Tnfaip1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi3877. Tnfaip1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Endosome By similarity

  • Note: Colocalizes with RHOB in endosomes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003312491 – 316BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2Add BLAST316

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei278PhosphoserineBy similarity1
Modified residuei280Phosphoserine; by CK2By similarity1

Post-translational modificationi

Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7TNY1.
PRIDEiQ7TNY1.

PTM databases

iPTMnetiQ7TNY1.
PhosphoSitePlusiQ7TNY1.

Expressioni

Gene expression databases

BgeeiENSRNOG00000009069.
GenevisibleiQ7TNY1. RN.

Interactioni

Subunit structurei

Component of the BCR(BACURD2) E3 ubiquitin ligase complex, at least composed of CUL3, TNFAIP1/BACURD2 and RBX1. Interacts with RHOA; with a preference for RhoA-GDP. Interacts with RHOB. Interacts with CSNK2B (By similarity). Interacts with PCNA.By similarity1 Publication

GO - Molecular functioni

  • GTP-Rho binding Source: UniProtKB
  • protein domain specific binding Source: RGD

Protein-protein interaction databases

BioGridi252226. 1 interactor.
STRINGi10116.ENSRNOP00000012245.

Structurei

3D structure databases

ProteinModelPortaliQ7TNY1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 96BTBAdd BLAST69

Sequence similaritiesi

Belongs to the BACURD family.Curated
Contains 1 BTB (POZ) domain.Curated

Phylogenomic databases

eggNOGiKOG2716. Eukaryota.
ENOG410XQYZ. LUCA.
GeneTreeiENSGT00530000063071.
HOVERGENiHBG052219.
InParanoidiQ7TNY1.
KOiK15074.
OMAiYESRNGP.
OrthoDBiEOG091G0L1Y.
PhylomeDBiQ7TNY1.
TreeFamiTF315649.

Family and domain databases

InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
[Graphical view]
PfamiPF02214. BTB_2. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7TNY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGDTCLCPA SGAKPKISGF KGGGLGNKYV QLNVGGSLHY TTVRALTRHD
60 70 80 90 100
TMLKAMFSGR MEVLTDKEGW ILIDRCGKHF GTILNYLRDD TVTLPQSRQE
110 120 130 140 150
IQELMAEAKY YLIQGLVSLC QAALQDKKDS YQPVCNIPII TSLREEDRLI
160 170 180 190 200
ESSTKPVVKL LYNRSNNKYS YTSNSDDHLL KNIELFDKLS LRFNGRVLFI
210 220 230 240 250
KDVIGDEICC WSFYGQGRKL AEVCCTSIVY ATEKKQTKVE FPEARIYEET
260 270 280 290 300
LNVLLYETPR VPDNSLLEAT SRSRSQASPS EDEDTFELRD RVRRIHVKRY
310
STYDDRQLGH QSAHRD
Length:316
Mass (Da):36,046
Last modified:October 1, 2003 - v1
Checksum:i9F55209382D736E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY336949 mRNA. Translation: AAP97077.1.
BC128704 mRNA. Translation: AAI28705.1.
RefSeqiNP_891995.1. NM_182950.4.
UniGeneiRn.18199.

Genome annotation databases

EnsembliENSRNOT00000012245; ENSRNOP00000012245; ENSRNOG00000009069.
GeneIDi287543.
KEGGirno:287543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY336949 mRNA. Translation: AAP97077.1.
BC128704 mRNA. Translation: AAI28705.1.
RefSeqiNP_891995.1. NM_182950.4.
UniGeneiRn.18199.

3D structure databases

ProteinModelPortaliQ7TNY1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi252226. 1 interactor.
STRINGi10116.ENSRNOP00000012245.

PTM databases

iPTMnetiQ7TNY1.
PhosphoSitePlusiQ7TNY1.

Proteomic databases

PaxDbiQ7TNY1.
PRIDEiQ7TNY1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012245; ENSRNOP00000012245; ENSRNOG00000009069.
GeneIDi287543.
KEGGirno:287543.

Organism-specific databases

CTDi7126.
RGDi3877. Tnfaip1.

Phylogenomic databases

eggNOGiKOG2716. Eukaryota.
ENOG410XQYZ. LUCA.
GeneTreeiENSGT00530000063071.
HOVERGENiHBG052219.
InParanoidiQ7TNY1.
KOiK15074.
OMAiYESRNGP.
OrthoDBiEOG091G0L1Y.
PhylomeDBiQ7TNY1.
TreeFamiTF315649.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ7TNY1.

Gene expression databases

BgeeiENSRNOG00000009069.
GenevisibleiQ7TNY1. RN.

Family and domain databases

InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
[Graphical view]
PfamiPF02214. BTB_2. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBACD2_RAT
AccessioniPrimary (citable) accession number: Q7TNY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 1, 2003
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.