Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Rho guanine nucleotide exchange factor 4

Gene

Arhgef4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion (By similarity). Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-193648. NRAGE signals death through JNK.
R-MMU-194840. Rho GTPase cycle.
R-MMU-416482. G alpha (12/13) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 4
Alternative name(s):
APC-stimulated guanine nucleotide exchange factor
Short name:
Asef
Gene namesi
Name:Arhgef4
Synonyms:Kiaa1112
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2442507. Arhgef4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484Rho guanine nucleotide exchange factor 4PRO_0000080915Add
BLAST

Proteomic databases

EPDiQ7TNR9.
MaxQBiQ7TNR9.
PaxDbiQ7TNR9.
PRIDEiQ7TNR9.

PTM databases

PhosphoSiteiQ7TNR9.

Expressioni

Tissue specificityi

Expressed in colon epithelial cells. Highly expressed in CNS, including hippocampus, olfactory bulb and cerebellum. Expression is aberrantly enhanced in most colorectal tumors.2 Publications

Gene expression databases

BgeeiQ7TNR9.
CleanExiMM_ARHGEF4.
ExpressionAtlasiQ7TNR9. baseline and differential.
GenevisibleiQ7TNR9. MM.

Interactioni

Subunit structurei

Interacts with RHOA and RAC1, and APC. Found in a complex consisting of ARHGEF4, APC and CTNNB1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035980.

Structurei

3D structure databases

ProteinModelPortaliQ7TNR9.
SMRiQ7TNR9. Positions 1-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 4646SH3PROSITE-ProRule annotationAdd
BLAST
Domaini78 – 262185DHPROSITE-ProRule annotationAdd
BLAST
Domaini293 – 400108PHPROSITE-ProRule annotationAdd
BLAST

Domaini

In an autoinhibited form the SH3 domain binds intramolecularly to the DH domain, thus blocking the Rac-binding site.By similarity

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiENOG410KDSK. Eukaryota.
ENOG410XQDV. LUCA.
GeneTreeiENSGT00760000118925.
HOGENOMiHOG000237363.
HOVERGENiHBG050568.
InParanoidiQ7TNR9.
KOiK05769.
PhylomeDBiQ7TNR9.
TreeFamiTF316832.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TNR9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDQELGFKA GDVIEVMDAT NREWWWGRVA DGEGWFPASF VRLRVNQDEP
60 70 80 90 100
ADDYEAPRAG AREADDSGPE AQSCKDQMRT NVINEILSTE RDYIKHLRDI
110 120 130 140 150
CEGYVRQCRK REDMFSEEQL RTIFGNIEDI YRCQKAFVKA LEQKFNTERP
160 170 180 190 200
HLSELGACFL EHQADFQIYS EYCNNHPNAC VELSRLTKLS KYVYFFEACR
210 220 230 240 250
LLQRMIDISL DGFLLTPVQK ICKYPLQLAE LLKYTHPQHR DFKNVEAALH
260 270 280 290 300
AMKNVAQLIN ERKRRLENID KIAQWQSSIE DWEGEDLLVR SSELIHSGEL
310 320 330 340 350
TRVTQPQARS QQRMFFLFDR QLIYCKKDLL RRDVLYYKGR LDMDDLEVVD
360 370 380 390 400
VEDGKDRDLH VSVKNAFRLY CGTTGDSHLL CARKPEQKQR WLKAFARERE
410 420 430 440 450
QVRLDQETGF SITELQRKQA MLNASKQQAT GKPKAVGRPG YLTRHKHPSL
460 470 480
PASRPQQQVL VLAEPRRKPS NFWHSISRLA PFRK
Length:484
Mass (Da):56,679
Last modified:September 27, 2004 - v2
Checksum:iD4DCDE8623F110FE
GO

Sequence cautioni

The sequence BC055804 differs from that shown. Reason: Frameshift at position 201. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC107738 Genomic DNA. No translation available.
BC055804 mRNA. No translation available.
AK122448 mRNA. Translation: BAC65730.2.
CCDSiCCDS14872.1.
RefSeqiNP_898840.2. NM_183019.2.
UniGeneiMm.41604.

Genome annotation databases

EnsembliENSMUST00000047664; ENSMUSP00000035980; ENSMUSG00000037509.
GeneIDi226970.
KEGGimmu:226970.
UCSCiuc007api.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC107738 Genomic DNA. No translation available.
BC055804 mRNA. No translation available.
AK122448 mRNA. Translation: BAC65730.2.
CCDSiCCDS14872.1.
RefSeqiNP_898840.2. NM_183019.2.
UniGeneiMm.41604.

3D structure databases

ProteinModelPortaliQ7TNR9.
SMRiQ7TNR9. Positions 1-405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035980.

PTM databases

PhosphoSiteiQ7TNR9.

Proteomic databases

EPDiQ7TNR9.
MaxQBiQ7TNR9.
PaxDbiQ7TNR9.
PRIDEiQ7TNR9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047664; ENSMUSP00000035980; ENSMUSG00000037509.
GeneIDi226970.
KEGGimmu:226970.
UCSCiuc007api.1. mouse.

Organism-specific databases

CTDi50649.
MGIiMGI:2442507. Arhgef4.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410KDSK. Eukaryota.
ENOG410XQDV. LUCA.
GeneTreeiENSGT00760000118925.
HOGENOMiHOG000237363.
HOVERGENiHBG050568.
InParanoidiQ7TNR9.
KOiK05769.
PhylomeDBiQ7TNR9.
TreeFamiTF316832.

Enzyme and pathway databases

ReactomeiR-MMU-193648. NRAGE signals death through JNK.
R-MMU-194840. Rho GTPase cycle.
R-MMU-416482. G alpha (12/13) signalling events.

Miscellaneous databases

PROiQ7TNR9.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TNR9.
CleanExiMM_ARHGEF4.
ExpressionAtlasiQ7TNR9. baseline and differential.
GenevisibleiQ7TNR9. MM.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 205-484.
    Tissue: Brain.
  4. Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  5. "Asef, a link between the tumor suppressor APC and G-protein signaling."
    Kawasaki Y., Senda T., Ishidate T., Koyama R., Morishita T., Iwayama Y., Higuchi O., Akiyama T.
    Science 289:1194-1197(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "The adenomatous polyposis coli-associated exchange factors Asef and Asef2 are required for adenoma formation in Apc(Min/+)mice."
    Kawasaki Y., Tsuji S., Muroya K., Furukawa S., Shibata Y., Okuno M., Ohwada S., Akiyama T.
    EMBO Rep. 10:1355-1362(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiARHG4_MOUSE
AccessioniPrimary (citable) accession number: Q7TNR9
Secondary accession number(s): Q80TJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: June 8, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.