Q7TNL3 (STK40_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase 40
EC=2.7.11.1

Alternative name(s):
Serine/threonine-protein kinase lyk4

Gene names

Name:	Stk40
Synonyms:	Lyk4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be a negative regulator of NF-kappa-B and p53-mediated gene transcription By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Sequence caution	The sequence AAH46981.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7TNL3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7TNL3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MKPYLRGASTQELRM

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Serine/threonine-protein kinase 40		PRO_0000252262
Regions
	Domain	35 – 332	298	Protein kinase
	Nucleotide binding	41 – 49	9	ATP By similarity
Sites
	Active site	197	1	Proton acceptor By similarity
	Binding site	66	1	ATP By similarity
Natural variations
	Alternative sequence	1	1	M → MKPYLRGASTQELRM in isoform 2.		VSP_020901
Experimental info
	Sequence conflict	196	1	R → L in BAB26110. Ref.3
	Sequence conflict	392	1	I → V in BAB26110. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 5AA58BA842CE2ACF	FASTA	435	48,932
	10 20 30 40 50 60 MKRRASDRGA GETSANAKAL GTGIAGNNAK RAGPFVLGPR LGNSPVPSIV QCLARKDGTD 70 80 90 100 110 120 DFYQLKILTL EERGEQGIES QEERQGKMLL HTEYSLLSLL HTQDGVVHHH GLFQDRTCEA 130 140 150 160 170 180 VEDTESGRMV KKMKKRICLV LDCLCAHDFS DKTADLINLQ HYVIKEKRLS ERETVVIFYD 190 200 210 220 230 240 VVRVVEALHQ KNIVHRDLKL GNMVLNKRTH RITITNFCLG KHLVSEGDLL KDQRGSPAYI 250 260 270 280 290 300 SPDVLSGRPY RGKPSDMWAL GVVLFTMLYG QFPFYDSIPQ ELFRKIKAAE YTIPEDGRVS 310 320 330 340 350 360 ENTVCLIRKL LVLDPQQRLA AADVLEALSA IIASWQSLSS LSGPLQVVPD IDDQMSSSDS 370 380 390 400 410 420 SQEAKVTEEC SQYEFENYMR QQLLLAEEKS SIHEARAWVP KRQFGSMPPV RRLGHDAQPM 430 TSLDTAILAQ RYLRK
	Isoform 2 [UniParc]. Checksum: DA5D43BE556E7B92	FASTA	449	50,564
	10 20 30 40 50 60 MKPYLRGAST QELRMKRRAS DRGAGETSAN AKALGTGIAG NNAKRAGPFV LGPRLGNSPV 70 80 90 100 110 120 PSIVQCLARK DGTDDFYQLK ILTLEERGEQ GIESQEERQG KMLLHTEYSL LSLLHTQDGV 130 140 150 160 170 180 VHHHGLFQDR TCEAVEDTES GRMVKKMKKR ICLVLDCLCA HDFSDKTADL INLQHYVIKE 190 200 210 220 230 240 KRLSERETVV IFYDVVRVVE ALHQKNIVHR DLKLGNMVLN KRTHRITITN FCLGKHLVSE 250 260 270 280 290 300 GDLLKDQRGS PAYISPDVLS GRPYRGKPSD MWALGVVLFT MLYGQFPFYD SIPQELFRKI 310 320 330 340 350 360 KAAEYTIPED GRVSENTVCL IRKLLVLDPQ QRLAAADVLE ALSAIIASWQ SLSSLSGPLQ 370 380 390 400 410 420 VVPDIDDQMS SSDSSQEAKV TEECSQYEFE NYMRQQLLLA EEKSSIHEAR AWVPKRQFGS 430 440 MPPVRRLGHD AQPMTSLDTA ILAQRYLRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of human, mouse, rat, chick and frog lyk4 gene." Shan Y.X., Yu L. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[2]	"Gene expression profiling during the embryonic development of mouse brain using an oligonucleotide-based microarray system." Matsuki T., Hori G., Furuichi T. Brain Res. Mol. Brain Res. 136:231-254(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J, ICR and NOD. Tissue: Brain and Tongue.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Colon.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY336057 mRNA. Translation: AAQ01591.1. AB102946 mRNA. Translation: BAC81568.1. AK009154 mRNA. Translation: BAB26110.2. AK050808 mRNA. Translation: BAC34419.2. AK154780 mRNA. Translation: BAE32823.1. AL731780 Genomic DNA. Translation: CAM22313.1. BC046981 mRNA. Translation: AAH46981.1. Different initiation.
IPI	IPI00380330. IPI00648951.
RefSeq	NP_001139299.1. NM_001145827.1. NP_083076.3. NM_028800.3.
UniGene	Mm.440269.
3D structure databases
ProteinModelPortal	Q7TNL3.
SMR	Q7TNL3. Positions 35-352.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-58526N.
STRING	10090.ENSMUSP00000111990.
PTM databases
PhosphoSite	Q7TNL3.
Proteomic databases
PaxDb	Q7TNL3.
PRIDE	Q7TNL3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000094761; ENSMUSP00000092354; ENSMUSG00000042608. ENSMUST00000116286; ENSMUSP00000111990; ENSMUSG00000042608.
GeneID	74178.
KEGG	mmu:74178.
UCSC	uc008usl.2. mouse. uc008usm.2. mouse.
Organism-specific databases
CTD	83931.
MGI	MGI:1921428. Stk40.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00700000104008.
HOGENOM	HOG000007322.
HOVERGEN	HBG080296.
InParanoid	B1AW01.
KO	K16312.
OMA	SSIIASW.
OrthoDB	EOG4ZPDV8.
Gene expression databases
ArrayExpress	Q7TNL3.
Bgee	Q7TNL3.
CleanEx	MM_STK40.
Genevestigator	Q7TNL3.
GermOnline	ENSMUSG00000042608. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR024236. Ser/Thr_kinase_40. IPR008271. Ser/Thr_kinase_AS. IPR024104. Tribbles/Ser_Thr_kinase_40. [Graphical view]
PANTHER	PTHR22961. PTHR22961. 1 hit. PTHR22961:SF1. PTHR22961:SF1. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	STK40. mouse.
NextBio	340002.
SOURCE	Search...

Entry information

Entry name

STK40_MOUSE

Accession

Primary (citable) accession number: Q7TNL3
Secondary accession number(s): B1AW01 , Q3U3G3, Q811D6, Q8BKT1, Q9D7K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	October 1, 2003
Last modified:	May 29, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program