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Q7TNJ7

- KCC1G_RAT

UniProt

Q7TNJ7 - KCC1G_RAT

Protein

Calcium/calmodulin-dependent protein kinase type 1G

Gene

Camk1g

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1 By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.1 Publication

    Enzyme regulationi

    Activated by Ca2+/calmodulin. Binding of calmodulin is thought to result in a conformational change and leads to activation through phosphorylation by CAMKK1 By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521ATPPROSITE-ProRule annotation
    Active sitei143 – 1431Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 379ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calmodulin-dependent protein kinase activity Source: RGD

    GO - Biological processi

    1. calcium-mediated signaling Source: RGD
    2. protein phosphorylation Source: RGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.17. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium/calmodulin-dependent protein kinase type 1G (EC:2.7.11.17)
    Alternative name(s):
    CaM kinase I gamma
    Short name:
    CaM kinase IG
    Short name:
    CaM-KI gamma
    Short name:
    CaMKI gamma
    Short name:
    CaMKIG
    CaMK-like CREB kinase III
    Short name:
    CLICK III
    Gene namesi
    Name:Camk1g
    Synonyms:Camkig
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 13

    Organism-specific databases

    RGDi621800. Camk1g.

    Subcellular locationi

    Cytoplasm By similarity. Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. calcium- and calmodulin-dependent protein kinase complex Source: Ensembl
    2. cytoplasm Source: RGD
    3. Golgi membrane Source: UniProtKB-SubCell
    4. neuron projection Source: RGD
    5. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 476476Calcium/calmodulin-dependent protein kinase type 1GPRO_0000086086Add
    BLAST

    Post-translational modificationi

    Prenylated on Cys-473.By similarity

    Keywords - PTMi

    Lipoprotein, Prenylation

    Proteomic databases

    PaxDbiQ7TNJ7.
    PRIDEiQ7TNJ7.

    Expressioni

    Gene expression databases

    GenevestigatoriQ7TNJ7.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7TNJ7.
    SMRiQ7TNJ7. Positions 16-301.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 277255Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni277 – 31741Autoinhibitory domainAdd
    BLAST
    Regioni297 – 31822Calmodulin-bindingAdd
    BLAST

    Domaini

    The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.By similarity

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000115341.
    HOGENOMiHOG000233016.
    HOVERGENiHBG108055.
    InParanoidiQ7TNJ7.
    KOiK08794.
    OMAiPWINGNT.
    OrthoDBiEOG7WHH9K.
    PhylomeDBiQ7TNJ7.
    TreeFamiTF314166.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 2 hits.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q7TNJ7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGRKEEEDCS SWKKQTTNIR KTFIFMEVLG SGAFSEVFLV KQRVTGKLFA    50
    LKCIKKSPAF RDSSLENEIA VLKRIKHENI VTLEDIYEST THYYLVMQLV 100
    SGGELFDRIL ERGVYTEKDA SLVIQQVLSA VKYLHENGIV HRDLKPENLL 150
    YLTPEENSKI MITDFGLSKM EQNGVMSTAC GTPGYVAPEV LAQKPYSKAV 200
    DCWSIGVITY ILLCGYPPFY EETESKLFEK IKEGYYEFES PFWDDISESA 250
    KDFICHLLEK DPNERYTCEK ALRHPWIDGN TALHRDIYPS VSLQIQKNFA 300
    KSKWRQAFNA AAVVHHMRKL HMNLHSPSVR QEVENRPPVS PAPEVSRPGS 350
    HDSSITEAPI LDPSTPLPAL TRLPCSHSSR PSAPGGRSLN CLVNGSLRIS 400
    SSLVPMQQGP LATGPCGCCS SCLNIGNKGK SSYCSEPTLF RKANKKQNFK 450
    SEVMVPVKAG GSTHCRAGQT GVCLIM 476
    Length:476
    Mass (Da):53,179
    Last modified:October 1, 2003 - v1
    Checksum:i70AD679B0C36B0E3
    GO
    Isoform 2 (identifier: Q7TNJ7-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         306-476: QAFNAAAVVH...AGQTGVCLIM → ELQVRGHGTS...GIAEAAGLRQ

    Show »
    Length:377
    Mass (Da):42,902
    Checksum:i2583EE864A5C12FC
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei306 – 476171QAFNA…VCLIM → ELQVRGHGTSESRWQHPLPG WADWGVSHNVIPGARVVFQE TRLALLCLSKLASALRKGSS KAGIAEAAGLRQ in isoform 2. 1 PublicationVSP_012139Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB101231 mRNA. Translation: BAC80242.1.
    AB101232 mRNA. Translation: BAC80243.1.
    D86557 mRNA. Translation: BAA19880.1.
    RefSeqiNP_878262.1. NM_182842.1. [Q7TNJ7-1]
    XP_006250532.1. XM_006250470.1. [Q7TNJ7-4]
    UniGeneiRn.91453.

    Genome annotation databases

    EnsembliENSRNOT00000009158; ENSRNOP00000009158; ENSRNOG00000006470. [Q7TNJ7-1]
    ENSRNOT00000044161; ENSRNOP00000041124; ENSRNOG00000006470. [Q7TNJ7-4]
    GeneIDi171358.
    KEGGirno:171358.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB101231 mRNA. Translation: BAC80242.1 .
    AB101232 mRNA. Translation: BAC80243.1 .
    D86557 mRNA. Translation: BAA19880.1 .
    RefSeqi NP_878262.1. NM_182842.1. [Q7TNJ7-1 ]
    XP_006250532.1. XM_006250470.1. [Q7TNJ7-4 ]
    UniGenei Rn.91453.

    3D structure databases

    ProteinModelPortali Q7TNJ7.
    SMRi Q7TNJ7. Positions 16-301.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q7TNJ7.
    PRIDEi Q7TNJ7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000009158 ; ENSRNOP00000009158 ; ENSRNOG00000006470 . [Q7TNJ7-1 ]
    ENSRNOT00000044161 ; ENSRNOP00000041124 ; ENSRNOG00000006470 . [Q7TNJ7-4 ]
    GeneIDi 171358.
    KEGGi rno:171358.

    Organism-specific databases

    CTDi 57172.
    RGDi 621800. Camk1g.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000115341.
    HOGENOMi HOG000233016.
    HOVERGENi HBG108055.
    InParanoidi Q7TNJ7.
    KOi K08794.
    OMAi PWINGNT.
    OrthoDBi EOG7WHH9K.
    PhylomeDBi Q7TNJ7.
    TreeFami TF314166.

    Enzyme and pathway databases

    BRENDAi 2.7.11.17. 5301.

    Miscellaneous databases

    NextBioi 622144.
    PROi Q7TNJ7.

    Gene expression databases

    Genevestigatori Q7TNJ7.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 2 hits.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, characterization and expression of two alternatively splicing isoforms of Ca2+/calmodulin-dependent protein kinase I gamma in the rat brain."
      Nishimura H., Sakagami H., Uezu A., Fukunaga K., Watanabe M., Kondo H.
      J. Neurochem. 85:1216-1227(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ENZYME ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    2. "Isolation and comparison of rat cDNAs encoding Ca2+/calmodulin-dependent protein kinase I isoforms."
      Yokokura H., Terada O., Naito Y., Hidaka H.
      Biochim. Biophys. Acta 1338:8-12(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-309.
      Tissue: Brain.

    Entry informationi

    Entry nameiKCC1G_RAT
    AccessioniPrimary (citable) accession number: Q7TNJ7
    Secondary accession number(s): O08763, Q7TNJ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 7, 2004
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3