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Protein

Amphoterin-induced protein 2

Gene

Amigo2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain.2 Publications

GO - Biological processi

  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: UniProtKB
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of programmed cell death Source: UniProtKB

Keywordsi

Biological processCell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Amphoterin-induced protein 2
Alternative name(s):
AMIGO-2
Alivin-1
Gene namesi
Name:Amigo2Imported
Synonyms:Ali1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727911. Amigo2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini38 – 398ExtracellularSequence analysisAdd BLAST361
Transmembranei399 – 419HelicalSequence analysisAdd BLAST21
Topological domaini420 – 520CytoplasmicSequence analysisAdd BLAST101

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Sequence analysisAdd BLAST37
ChainiPRO_000001451238 – 520Amphoterin-induced protein 2Sequence analysisAdd BLAST483

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 46PROSITE-ProRule annotation
Disulfide bondi44 ↔ 53PROSITE-ProRule annotation
Glycosylationi57N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi103N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi231 ↔ 259PROSITE-ProRule annotation
Disulfide bondi233 ↔ 281PROSITE-ProRule annotation
Glycosylationi280N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi287N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi309 ↔ 362PROSITE-ProRule annotation
Glycosylationi344N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi372N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi380N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi383N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi387N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ7TNJ4.
PRIDEiQ7TNJ4.

Expressioni

Tissue specificityi

Highest levels in the lung. High levels in cerebellar granule neurons and Purkinje cells. Also in pyramidal cells between CA1 and CA3 regions of the hippocampus and granule cells of the dentate gyrus.2 Publications

Developmental stagei

High level expression in cerebellum of newborn rats is down-regulated to 50% by postnatal day 14.1 Publication

Interactioni

Subunit structurei

Binds itself as well as AMIGO1 and AMIGO3.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ7TNJ4.
SMRiQ7TNJ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 67LRRNTAdd BLAST30
Repeati68 – 89LRR 1Add BLAST22
Repeati93 – 114LRR 2Add BLAST22
Repeati117 – 138LRR 3Add BLAST22
Repeati141 – 162LRR 4Add BLAST22
Repeati165 – 186LRR 5Add BLAST22
Repeati192 – 213LRR 6Add BLAST22
Domaini227 – 283LRRCTAdd BLAST57
Domaini288 – 378Ig-like C2-typeSequence analysisAdd BLAST91

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGN4. Eukaryota.
ENOG410ZZH4. LUCA.
HOGENOMiHOG000231327.
HOVERGENiHBG080231.
InParanoidiQ7TNJ4.
PhylomeDBiQ7TNJ4.
TreeFamiTF326838.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 1 hit.
InterProiView protein in InterPro
IPR031283. AMIGO.
IPR031286. AMIGO2.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013151. Immunoglobulin.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
PANTHERiPTHR24368. PTHR24368. 1 hit.
PTHR24368:SF216. PTHR24368:SF216. 1 hit.
PfamiView protein in Pfam
PF00047. ig. 1 hit.
PF13855. LRR_8. 1 hit.
SMARTiView protein in SMART
SM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00369. LRR_TYP. 5 hits.
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiView protein in PROSITE
PS50835. IG_LIKE. 1 hit.
PS51450. LRR. 5 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7TNJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRFHTLPT LPRAVKPGCR ELLCLLVIAV MVSPSSSGLC PTACICATDI
60 70 80 90 100
VSCTNKNLSK VPGNLFRLIK RLDLSYNRIG LLDADWIPVS FVKLSTLIVR
110 120 130 140 150
HNNITSISTG SFSTTPNLKC LDLSSNRLKS VKSAMFQELK VLEVLLLYNN
160 170 180 190 200
HISYLDPAAF GGLSHLQKLY LSGNFLTKFP MDLYVGRFKL ADLTFLDVSY
210 220 230 240 250
NQIASIPMHH INLVPGKQLR GIFLHGNPFV CDCSLYSLLT FWYRRHFNSV
260 270 280 290 300
TDFKHDYTCR LWLDSRHSHQ LLLLQDSFLN CSHSVINGSF HALGFIHEAQ
310 320 330 340 350
VGERAIVHCD GKTGNGNTDF IWVGPDNRLL EPDKDTGNFR VFYNGSLVIE
360 370 380 390 400
NPGFEDAGVY SCIAMNRQRL LNETVDIMIN VSNFTINRSH HAHEAFNTAF
410 420 430 440 450
TTLAACVVSI VLVLLYLYLT PCPCKCRDKR QKNALNQSNA HSSILSPGPT
460 470 480 490 500
RDASAEDRKA GKRVVFLEPL KDPAVGQNGK VKLVPSKTVI AEGILKSSRA
510 520
KSDSDSVNSV FSDTPFVAST
Length:520
Mass (Da):57,759
Last modified:October 1, 2003 - v1
Checksum:i9C0B8009DB35089B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti487K → E in AAO48951 (PubMed:12843293).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY237730 mRNA. Translation: AAO48951.1.
AB078879 mRNA. Translation: BAC81186.1.
RefSeqiNP_877968.2. NM_182816.2.
XP_006242352.1. XM_006242290.2.
XP_017450269.1. XM_017594780.1.
XP_017450270.1. XM_017594781.1.
UniGeneiRn.98123.

Genome annotation databases

GeneIDi300186.
KEGGirno:300186.
UCSCiRGD:727911. rat.

Similar proteinsi

Entry informationi

Entry nameiAMGO2_RAT
AccessioniPrimary (citable) accession number: Q7TNJ4
Secondary accession number(s): Q80ZD6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 1, 2003
Last modified: August 30, 2017
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families