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Protein

Amphoterin-induced protein 2

Gene

Amigo2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain.2 Publications

GO - Biological processi

  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: UniProtKB
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of programmed cell death Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Amphoterin-induced protein 2
Alternative name(s):
AMIGO-2
Alivin-1
Gene namesi
Name:Amigo2Imported
Synonyms:Ali1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727911. Amigo2.

Subcellular locationi

  • Cell membrane Curated; Single-pass type I membrane protein Curated
  • Nucleus 1 Publication

  • Note: Associated with nucleus as well as plasma membrane. Restricted to somata of cerebellar as well as hippocampal neurons.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini38 – 398361ExtracellularSequence analysisAdd
BLAST
Transmembranei399 – 41921HelicalSequence analysisAdd
BLAST
Topological domaini420 – 520101CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3737Sequence analysisAdd
BLAST
Chaini38 – 520483Amphoterin-induced protein 2Sequence analysisPRO_0000014512Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi40 ↔ 46PROSITE-ProRule annotation
Disulfide bondi44 ↔ 53PROSITE-ProRule annotation
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence analysis
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence analysis
Disulfide bondi231 ↔ 259PROSITE-ProRule annotation
Disulfide bondi233 ↔ 281PROSITE-ProRule annotation
Glycosylationi280 – 2801N-linked (GlcNAc...)Sequence analysis
Glycosylationi287 – 2871N-linked (GlcNAc...)Sequence analysis
Disulfide bondi309 ↔ 362PROSITE-ProRule annotation
Glycosylationi344 – 3441N-linked (GlcNAc...)Sequence analysis
Glycosylationi372 – 3721N-linked (GlcNAc...)Sequence analysis
Glycosylationi380 – 3801N-linked (GlcNAc...)Sequence analysis
Glycosylationi383 – 3831N-linked (GlcNAc...)Sequence analysis
Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ7TNJ4.
PRIDEiQ7TNJ4.

Expressioni

Tissue specificityi

Highest levels in the lung. High levels in cerebellar granule neurons and Purkinje cells. Also in pyramidal cells between CA1 and CA3 regions of the hippocampus and granule cells of the dentate gyrus.2 Publications

Developmental stagei

High level expression in cerebellum of newborn rats is down-regulated to 50% by postnatal day 14.1 Publication

Gene expression databases

BgeeiENSRNOG00000007032.

Interactioni

Subunit structurei

Binds itself as well as AMIGO1 and AMIGO3.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009199.

Structurei

3D structure databases

ProteinModelPortaliQ7TNJ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 6730LRRNTAdd
BLAST
Repeati68 – 8922LRR 1Add
BLAST
Repeati93 – 11422LRR 2Add
BLAST
Repeati117 – 13822LRR 3Add
BLAST
Repeati141 – 16222LRR 4Add
BLAST
Repeati165 – 18622LRR 5Add
BLAST
Repeati192 – 21322LRR 6Add
BLAST
Domaini227 – 28357LRRCTAdd
BLAST
Domaini288 – 37891Ig-like C2-typeSequence analysisAdd
BLAST

Sequence similaritiesi

Contains 6 LRR (leucine-rich) repeats.Sequence analysis
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Immunoglobulin domain, Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGN4. Eukaryota.
ENOG410ZZH4. LUCA.
HOGENOMiHOG000231327.
HOVERGENiHBG080231.
InParanoidiQ7TNJ4.
PhylomeDBiQ7TNJ4.
TreeFamiTF326838.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR031283. AMIGO.
IPR031286. AMIGO2.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013151. Immunoglobulin.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PANTHERiPTHR24368. PTHR24368. 1 hit.
PTHR24368:SF209. PTHR24368:SF209. 1 hit.
PfamiPF00047. ig. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00369. LRR_TYP. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51450. LRR. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7TNJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRFHTLPT LPRAVKPGCR ELLCLLVIAV MVSPSSSGLC PTACICATDI
60 70 80 90 100
VSCTNKNLSK VPGNLFRLIK RLDLSYNRIG LLDADWIPVS FVKLSTLIVR
110 120 130 140 150
HNNITSISTG SFSTTPNLKC LDLSSNRLKS VKSAMFQELK VLEVLLLYNN
160 170 180 190 200
HISYLDPAAF GGLSHLQKLY LSGNFLTKFP MDLYVGRFKL ADLTFLDVSY
210 220 230 240 250
NQIASIPMHH INLVPGKQLR GIFLHGNPFV CDCSLYSLLT FWYRRHFNSV
260 270 280 290 300
TDFKHDYTCR LWLDSRHSHQ LLLLQDSFLN CSHSVINGSF HALGFIHEAQ
310 320 330 340 350
VGERAIVHCD GKTGNGNTDF IWVGPDNRLL EPDKDTGNFR VFYNGSLVIE
360 370 380 390 400
NPGFEDAGVY SCIAMNRQRL LNETVDIMIN VSNFTINRSH HAHEAFNTAF
410 420 430 440 450
TTLAACVVSI VLVLLYLYLT PCPCKCRDKR QKNALNQSNA HSSILSPGPT
460 470 480 490 500
RDASAEDRKA GKRVVFLEPL KDPAVGQNGK VKLVPSKTVI AEGILKSSRA
510 520
KSDSDSVNSV FSDTPFVAST
Length:520
Mass (Da):57,759
Last modified:October 1, 2003 - v1
Checksum:i9C0B8009DB35089B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti487 – 4871K → E in AAO48951 (PubMed:12843293).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY237730 mRNA. Translation: AAO48951.1.
AB078879 mRNA. Translation: BAC81186.1.
RefSeqiNP_877968.2. NM_182816.2.
XP_006242352.1. XM_006242290.1.
UniGeneiRn.98123.

Genome annotation databases

GeneIDi300186.
KEGGirno:300186.
UCSCiRGD:727911. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY237730 mRNA. Translation: AAO48951.1.
AB078879 mRNA. Translation: BAC81186.1.
RefSeqiNP_877968.2. NM_182816.2.
XP_006242352.1. XM_006242290.1.
UniGeneiRn.98123.

3D structure databases

ProteinModelPortaliQ7TNJ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009199.

Proteomic databases

PaxDbiQ7TNJ4.
PRIDEiQ7TNJ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi300186.
KEGGirno:300186.
UCSCiRGD:727911. rat.

Organism-specific databases

CTDi347902.
RGDi727911. Amigo2.

Phylogenomic databases

eggNOGiENOG410IGN4. Eukaryota.
ENOG410ZZH4. LUCA.
HOGENOMiHOG000231327.
HOVERGENiHBG080231.
InParanoidiQ7TNJ4.
PhylomeDBiQ7TNJ4.
TreeFamiTF326838.

Miscellaneous databases

PROiQ7TNJ4.

Gene expression databases

BgeeiENSRNOG00000007032.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR031283. AMIGO.
IPR031286. AMIGO2.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013151. Immunoglobulin.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PANTHERiPTHR24368. PTHR24368. 1 hit.
PTHR24368:SF209. PTHR24368:SF209. 1 hit.
PfamiPF00047. ig. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00369. LRR_TYP. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF52058. SSF52058. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51450. LRR. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMGO2_RAT
AccessioniPrimary (citable) accession number: Q7TNJ4
Secondary accession number(s): Q80ZD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 1, 2003
Last modified: September 7, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.