Reviewed, UniProtKB/Swiss-Prot Q7TNG8 (LDHD_MOUSE)

Names and origin

Protein names

Recommended name:
    Probable D-lactate dehydrogenase, mitochondrial
      Short name=Lactate dehydrogenase D
      Short name=DLD
    EC=1.1.2.4

Gene names

Name:

Ldhd

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c.
Cofactor	FAD By similarity.
Subunit structure	Interacts with CSRP3.
Subcellular location	Mitochondrion.
Tissue specificity	Readily detected in liver and kidney, with a weaker signal observed in heart, skeletal muscle, stomach, brain, and lung.
Sequence similarities	Belongs to the FAD-binding oxidoreductase/transferase type 4 family. Contains 1 FAD-binding PCMH-type domain.
Sequence caution	The sequence AAM50323.1 differs from that shown. Reason: Frameshift at positions 459 and 474. The sequence BAC29917.1 differs from that shown. Reason: Frameshift at positions 141, 229 and 315.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	ATP biosynthetic process Ref.1 Non-traceable author statement. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: UniProtKB
Molecular function	D-lactate dehydrogenase (cytochrome) activity Inferred from electronic annotation. Source: EC D-lactate dehydrogenase activity Ref.1 Non-traceable author statement. Source: UniProtKB FAD binding Inferred from electronic annotation. Source: InterPro protein binding Ref.1 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 484		Probable D-lactate dehydrogenase, mitochondrial		PRO_0000262953
Regions
	Domain	62 – 242	181	FAD-binding PCMH-type
Experimental info
	Sequence conflict	413	1	D → H in BAC29917. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q7TNG8-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 78BDF31A861B9A82	FASTA	484	51,848
	10 20 30 40 50 60 MAMLLRVATQ RLSPWRSFCS RGSQGGLSQD FVEALKAVVG SPHVSTASAV REQHGHDESM 70 80 90 100 110 120 HRCQPPDAVV WPQNVDQVSR VASLCYNQGV PIIPFGTGTG VEGGVCAVQG GVCINLTHMD 130 140 150 160 170 180 QITELNTEDF SVVVEPGVTR KALNTHLRDS GLWFPVDPGA DASLCGMAAT GASGTNAVRY 190 200 210 220 230 240 GTMRDNVINL EVVLPDGRLL HTAGRGRHYR KSAAGYNLTG LFVGSEGTLG IITSTTLRLH 250 260 270 280 290 300 PAPEATVAAT CAFPSVQAAV DSTVQILQAA VPVARIEFLD DVMMDACNRH SKLNCPVAPT 310 320 330 340 350 360 LFLEFHGSQQ TLAEQLQRTE AITQDNGGSH FSWAKEAEKR NELWAARHNA WYAALALSPG 370 380 390 400 410 420 SKAYSTDVCV PISRLPEILV ETKEEIKASK LTGAIVGHVG DGNFHCILLV DPDDAEEQRR 430 440 450 460 470 480 VKAFAENLGR RALALGGTCT GEHGIGLGKR QLLQEEVGPV GVETMRQLKN TLDPRGLMNP GKVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of putative mammalian D-lactate dehydrogenase enzymes." Flick M.J., Konieczny S.F. Biochem. Biophys. Res. Commun. 295:910-916(2002) [PubMed: 12127981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CSRP3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: 129/Sv.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 286-430. Strain: C57BL/6J. Tissue: Thymus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY092768 mRNA. Translation: AAM50323.1. Frameshift. BC039155 mRNA. Translation: AAH39155.1. BC055443 mRNA. Translation: AAH55443.1. AK037996 mRNA. Translation: BAC29917.1. Frameshift.
RefSeq	NP_081846.3.
UniGene	Mm.271578 Mm.27589
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000031958. Mus musculus. [Contig view]
GeneID	52815.
KEGG	mmu:52815.
Organism-specific databases
MGI	MGI:106428. Ldhd.
Phylogenomic databases
HOVERGEN	Q7TNG8.
Gene expression databases
ArrayExpress	Q7TNG8.
CleanEx	MM_LDHD.
GermOnline	ENSMUSG00000031958. Mus musculus.
Family and domain databases
InterPro	IPR016167. FAD-bd_2_sub1. IPR016168. FAD-linked_Oxase_FAD-bd_sub2. IPR004113. FAD-linked_oxidase_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.30.465.20. FAD-linked_oxidase_FAD-bd_sub2. 1 hit.
Pfam	PF02913. FAD-oxidase_C. 1 hit. PF01565. FAD_binding_4. 1 hit. [Graphical view]
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	309579.
SOURCE	Search...

Entry information

Entry name

LDHD_MOUSE

Accession

Primary (citable) accession number: Q7TNG8
Secondary accession number(s): Q8BYU7, Q8CIV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 1, 2003
Last modified:	November 25, 2008
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)