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Protein

Echinoderm microtubule-associated protein-like 2

Gene

Eml2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Tubulin binding protein that inhibits microtubule nucleation and growth, resulting in shorter microtubules.By similarity

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Echinoderm microtubule-associated protein-like 2
Short name:
EMAP-2
Gene namesi
Name:Eml2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1919455. Eml2.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002843881 – 649Echinoderm microtubule-associated protein-like 2Add BLAST649

Proteomic databases

EPDiQ7TNG5.
PaxDbiQ7TNG5.
PeptideAtlasiQ7TNG5.
PRIDEiQ7TNG5.

PTM databases

iPTMnetiQ7TNG5.
PhosphoSitePlusiQ7TNG5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040811.
CleanExiMM_EML2.
ExpressionAtlasiQ7TNG5. baseline and differential.
GenevisibleiQ7TNG5. MM.

Interactioni

Subunit structurei

Binds unpolymerized tubulins via its WD repeat region. Interacts with GRID2 and may also interact with GRID1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ7TNG5. 1 interactor.
MINTiMINT-5085024.
STRINGi10090.ENSMUSP00000037654.

Structurei

3D structure databases

ProteinModelPortaliQ7TNG5.
SMRiQ7TNG5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati103 – 152WD 1Add BLAST50
Repeati157 – 200WD 2Add BLAST44
Repeati203 – 242WD 3Add BLAST40
Repeati250 – 288WD 4Add BLAST39
Repeati292 – 331WD 5Add BLAST40
Repeati375 – 414WD 6Add BLAST40
Repeati416 – 455WD 7Add BLAST40
Repeati458 – 497WD 8Add BLAST40
Repeati504 – 543WD 9Add BLAST40
Repeati571 – 610WD 10Add BLAST40
Repeati616 – 649WD 11Add BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 649Tandem atypical propeller in EMLsBy similarityAdd BLAST640

Domaini

Contains a tandem atypical propeller in EMLs (TAPE) domain. The N-terminal beta-propeller is formed by canonical WD repeats; in contrast, the second beta-propeller contains one blade that is formed by discontinuous parts of the polypeptide chain (By similarity).By similarity

Sequence similaritiesi

Belongs to the WD repeat EMAP family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2106. Eukaryota.
ENOG410XNTQ. LUCA.
GeneTreeiENSGT00550000074369.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ7TNG5.
KOiK18595.
TreeFamiTF317832.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiView protein in InterPro
IPR005108. HELP.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
PfamiView protein in Pfam
PF03451. HELP. 1 hit.
PF00400. WD40. 6 hits.
SMARTiView protein in SMART
SM00320. WD40. 11 hits.
SUPFAMiSSF50998. SSF50998. 5 hits.
PROSITEiView protein in PROSITE
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TNG5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSFGIGKTK EVIFSMEEGS VKMFLRGRPV PMLIPDELAP TYSLDTRSEL
60 70 80 90 100
PSSRLKLDWV YGYRGRDCRA NLYLLPTGEV VYFVASVAVL YSVEEQRQRH
110 120 130 140 150
YLGHNDDIKC LAVHPDMVTI ATGQVAGTTK EGKPLPPHVR VWDSVSLSTL
160 170 180 190 200
HVLGLGVFDR AVCCVAFSKS NGGNLLCAVD ESNDHVLSVW DWAKESKVVD
210 220 230 240 250
SKCSNEAVLV ATFHPTDPSL LITCGKSHIY FWSLEGGSLS KRQGLFEKHE
260 270 280 290 300
KPKYVLCVTF LEGGDVVTGD SGGNLYVWGK GGNRITQEVQ GAHDGGVFAL
310 320 330 340 350
CALRDGTLVS GGGRDRRVVL WGSDYSKVQE VEVPEDFGPV RTVAEGRGDT
360 370 380 390 400
LYVGTTRNSI LLGSVHTGFS LLVQGHVEEL WGLATHPSRA QFVTCGQDKL
410 420 430 440 450
VHLWSSETHQ PVWSRSIEDP ARSAGFHPSG SVLAVGTVTG RWLLLDTETH
460 470 480 490 500
DLVAIHTDGN EQISVVSFSP DGAYLAVGSH DNLVYVYTVD QGGRKVSRLG
510 520 530 540 550
KCSGHSSFIT HLDWAQDSTC FVTNSGDYEI LYWDPVTCKQ ITSADTVRNV
560 570 580 590 600
EWATATCVLG FGVFGIWPEG ADGTDINAVA RSHDGKLLVS ADDFGKVHLF
610 620 630 640
SYPCCQPRAL SHKYGGHSSH VTNVAFLWDD SMALTTGGKD TSVLQWRVA
Length:649
Mass (Da):70,734
Last modified:October 1, 2003 - v1
Checksum:i7B85AE89FE9ECAAC
GO
Isoform 2 (identifier: Q7TNG5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     61-61: Y → YPLTSSLSALLEEALGFSSY

Note: No experimental confirmation available.
Show »
Length:668
Mass (Da):72,701
Checksum:iD81FF853F3E7665D
GO

Sequence cautioni

The sequence BAB24122 differs from that shown. Reason: Frameshift at position 32.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti251K → R in BAB24122 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02448061Y → YPLTSSLSALLEEALGFSSY in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005562 mRNA. Translation: BAB24122.1. Frameshift.
BC055476 mRNA. Translation: AAH55476.1.
CCDSiCCDS52055.1. [Q7TNG5-2]
CCDS85224.1. [Q7TNG5-1]
RefSeqiNP_001156468.1. NM_001162996.1. [Q7TNG5-1]
NP_082429.1. NM_028153.1. [Q7TNG5-2]
UniGeneiMm.154121.

Genome annotation databases

EnsembliENSMUST00000048502; ENSMUSP00000037654; ENSMUSG00000040811. [Q7TNG5-2]
ENSMUST00000120595; ENSMUSP00000112447; ENSMUSG00000040811. [Q7TNG5-1]
GeneIDi72205.
KEGGimmu:72205.
UCSCiuc009fkz.2. mouse. [Q7TNG5-1]
uc009fla.2. mouse. [Q7TNG5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005562 mRNA. Translation: BAB24122.1. Frameshift.
BC055476 mRNA. Translation: AAH55476.1.
CCDSiCCDS52055.1. [Q7TNG5-2]
CCDS85224.1. [Q7TNG5-1]
RefSeqiNP_001156468.1. NM_001162996.1. [Q7TNG5-1]
NP_082429.1. NM_028153.1. [Q7TNG5-2]
UniGeneiMm.154121.

3D structure databases

ProteinModelPortaliQ7TNG5.
SMRiQ7TNG5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7TNG5. 1 interactor.
MINTiMINT-5085024.
STRINGi10090.ENSMUSP00000037654.

PTM databases

iPTMnetiQ7TNG5.
PhosphoSitePlusiQ7TNG5.

Proteomic databases

EPDiQ7TNG5.
PaxDbiQ7TNG5.
PeptideAtlasiQ7TNG5.
PRIDEiQ7TNG5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048502; ENSMUSP00000037654; ENSMUSG00000040811. [Q7TNG5-2]
ENSMUST00000120595; ENSMUSP00000112447; ENSMUSG00000040811. [Q7TNG5-1]
GeneIDi72205.
KEGGimmu:72205.
UCSCiuc009fkz.2. mouse. [Q7TNG5-1]
uc009fla.2. mouse. [Q7TNG5-2]

Organism-specific databases

CTDi24139.
MGIiMGI:1919455. Eml2.

Phylogenomic databases

eggNOGiKOG2106. Eukaryota.
ENOG410XNTQ. LUCA.
GeneTreeiENSGT00550000074369.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ7TNG5.
KOiK18595.
TreeFamiTF317832.

Miscellaneous databases

PROiQ7TNG5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040811.
CleanExiMM_EML2.
ExpressionAtlasiQ7TNG5. baseline and differential.
GenevisibleiQ7TNG5. MM.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiView protein in InterPro
IPR005108. HELP.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
PfamiView protein in Pfam
PF03451. HELP. 1 hit.
PF00400. WD40. 6 hits.
SMARTiView protein in SMART
SM00320. WD40. 11 hits.
SUPFAMiSSF50998. SSF50998. 5 hits.
PROSITEiView protein in PROSITE
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEMAL2_MOUSE
AccessioniPrimary (citable) accession number: Q7TNG5
Secondary accession number(s): Q9DAS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: October 1, 2003
Last modified: February 15, 2017
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.