Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Echinoderm microtubule-associated protein-like 2

Gene

Eml2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Tubulin binding protein that inhibits microtubule nucleation and growth, resulting in shorter microtubules.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Echinoderm microtubule-associated protein-like 2
Short name:
EMAP-2
Gene namesi
Name:Eml2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1919455. Eml2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 649649Echinoderm microtubule-associated protein-like 2PRO_0000284388Add
BLAST

Proteomic databases

EPDiQ7TNG5.
MaxQBiQ7TNG5.
PaxDbiQ7TNG5.
PRIDEiQ7TNG5.

PTM databases

PhosphoSiteiQ7TNG5.

Expressioni

Gene expression databases

BgeeiQ7TNG5.
CleanExiMM_EML2.
ExpressionAtlasiQ7TNG5. baseline and differential.
GenevisibleiQ7TNG5. MM.

Interactioni

Subunit structurei

Binds unpolymerized tubulins via its WD repeat region. Interacts with GRID2 and may also interact with GRID1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ7TNG5. 1 interaction.
MINTiMINT-5085024.
STRINGi10090.ENSMUSP00000037654.

Structurei

3D structure databases

ProteinModelPortaliQ7TNG5.
SMRiQ7TNG5. Positions 10-648.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati103 – 15250WD 1Add
BLAST
Repeati157 – 20044WD 2Add
BLAST
Repeati203 – 24240WD 3Add
BLAST
Repeati250 – 28839WD 4Add
BLAST
Repeati292 – 33140WD 5Add
BLAST
Repeati375 – 41440WD 6Add
BLAST
Repeati416 – 45540WD 7Add
BLAST
Repeati458 – 49740WD 8Add
BLAST
Repeati504 – 54340WD 9Add
BLAST
Repeati571 – 61040WD 10Add
BLAST
Repeati616 – 64934WD 11Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 649640Tandem atypical propeller in EMLsBy similarityAdd
BLAST

Domaini

Contains a tandem atypical propeller in EMLs (TAPE) domain. The N-terminal beta-propeller is formed by canonical WD repeats; in contrast, the second beta-propeller contains one blade that is formed by discontinuous parts of the polypeptide chain (By similarity).By similarity

Sequence similaritiesi

Belongs to the WD repeat EMAP family.Curated
Contains 11 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2106. Eukaryota.
ENOG410XNTQ. LUCA.
GeneTreeiENSGT00550000074369.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ7TNG5.
KOiK18595.
TreeFamiTF317832.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR033016. EML2.
IPR005108. HELP.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR13720:SF32. PTHR13720:SF32. 1 hit.
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
SMARTiSM00320. WD40. 11 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 5 hits.
PROSITEiPS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TNG5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSFGIGKTK EVIFSMEEGS VKMFLRGRPV PMLIPDELAP TYSLDTRSEL
60 70 80 90 100
PSSRLKLDWV YGYRGRDCRA NLYLLPTGEV VYFVASVAVL YSVEEQRQRH
110 120 130 140 150
YLGHNDDIKC LAVHPDMVTI ATGQVAGTTK EGKPLPPHVR VWDSVSLSTL
160 170 180 190 200
HVLGLGVFDR AVCCVAFSKS NGGNLLCAVD ESNDHVLSVW DWAKESKVVD
210 220 230 240 250
SKCSNEAVLV ATFHPTDPSL LITCGKSHIY FWSLEGGSLS KRQGLFEKHE
260 270 280 290 300
KPKYVLCVTF LEGGDVVTGD SGGNLYVWGK GGNRITQEVQ GAHDGGVFAL
310 320 330 340 350
CALRDGTLVS GGGRDRRVVL WGSDYSKVQE VEVPEDFGPV RTVAEGRGDT
360 370 380 390 400
LYVGTTRNSI LLGSVHTGFS LLVQGHVEEL WGLATHPSRA QFVTCGQDKL
410 420 430 440 450
VHLWSSETHQ PVWSRSIEDP ARSAGFHPSG SVLAVGTVTG RWLLLDTETH
460 470 480 490 500
DLVAIHTDGN EQISVVSFSP DGAYLAVGSH DNLVYVYTVD QGGRKVSRLG
510 520 530 540 550
KCSGHSSFIT HLDWAQDSTC FVTNSGDYEI LYWDPVTCKQ ITSADTVRNV
560 570 580 590 600
EWATATCVLG FGVFGIWPEG ADGTDINAVA RSHDGKLLVS ADDFGKVHLF
610 620 630 640
SYPCCQPRAL SHKYGGHSSH VTNVAFLWDD SMALTTGGKD TSVLQWRVA
Length:649
Mass (Da):70,734
Last modified:October 1, 2003 - v1
Checksum:i7B85AE89FE9ECAAC
GO
Isoform 2 (identifier: Q7TNG5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     61-61: Y → YPLTSSLSALLEEALGFSSY

Note: No experimental confirmation available.
Show »
Length:668
Mass (Da):72,701
Checksum:iD81FF853F3E7665D
GO

Sequence cautioni

The sequence BAB24122.1 differs from that shown. Reason: Frameshift at position 32. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti251 – 2511K → R in BAB24122 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei61 – 611Y → YPLTSSLSALLEEALGFSSY in isoform 2. 1 PublicationVSP_024480

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005562 mRNA. Translation: BAB24122.1. Frameshift.
BC055476 mRNA. Translation: AAH55476.1.
CCDSiCCDS52055.1. [Q7TNG5-2]
RefSeqiNP_001156468.1. NM_001162996.1. [Q7TNG5-1]
NP_082429.1. NM_028153.1. [Q7TNG5-2]
UniGeneiMm.154121.

Genome annotation databases

EnsembliENSMUST00000048502; ENSMUSP00000037654; ENSMUSG00000040811. [Q7TNG5-2]
ENSMUST00000120595; ENSMUSP00000112447; ENSMUSG00000040811. [Q7TNG5-1]
GeneIDi72205.
KEGGimmu:72205.
UCSCiuc009fkz.2. mouse. [Q7TNG5-1]
uc009fla.2. mouse. [Q7TNG5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005562 mRNA. Translation: BAB24122.1. Frameshift.
BC055476 mRNA. Translation: AAH55476.1.
CCDSiCCDS52055.1. [Q7TNG5-2]
RefSeqiNP_001156468.1. NM_001162996.1. [Q7TNG5-1]
NP_082429.1. NM_028153.1. [Q7TNG5-2]
UniGeneiMm.154121.

3D structure databases

ProteinModelPortaliQ7TNG5.
SMRiQ7TNG5. Positions 10-648.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7TNG5. 1 interaction.
MINTiMINT-5085024.
STRINGi10090.ENSMUSP00000037654.

PTM databases

PhosphoSiteiQ7TNG5.

Proteomic databases

EPDiQ7TNG5.
MaxQBiQ7TNG5.
PaxDbiQ7TNG5.
PRIDEiQ7TNG5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048502; ENSMUSP00000037654; ENSMUSG00000040811. [Q7TNG5-2]
ENSMUST00000120595; ENSMUSP00000112447; ENSMUSG00000040811. [Q7TNG5-1]
GeneIDi72205.
KEGGimmu:72205.
UCSCiuc009fkz.2. mouse. [Q7TNG5-1]
uc009fla.2. mouse. [Q7TNG5-2]

Organism-specific databases

CTDi24139.
MGIiMGI:1919455. Eml2.

Phylogenomic databases

eggNOGiKOG2106. Eukaryota.
ENOG410XNTQ. LUCA.
GeneTreeiENSGT00550000074369.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ7TNG5.
KOiK18595.
TreeFamiTF317832.

Miscellaneous databases

NextBioi335691.
PROiQ7TNG5.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TNG5.
CleanExiMM_EML2.
ExpressionAtlasiQ7TNG5. baseline and differential.
GenevisibleiQ7TNG5. MM.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR033016. EML2.
IPR005108. HELP.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR13720:SF32. PTHR13720:SF32. 1 hit.
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
SMARTiSM00320. WD40. 11 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 5 hits.
PROSITEiPS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Placenta.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Colon.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiEMAL2_MOUSE
AccessioniPrimary (citable) accession number: Q7TNG5
Secondary accession number(s): Q9DAS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: October 1, 2003
Last modified: May 11, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.