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Protein

Double C2-like domain-containing protein alpha

Gene

Doc2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Calcium sensor which most probably regulates fusion of vesicles with membranes. Binds calcium and phospholipids. May be involved in calcium dependent neurotransmitter release through the interaction with UNC13A. May be involved in calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Regulates Ca2+-dependent secretory lysosome exocytosis in mast cells.2 Publications

GO - Molecular functioni

GO - Biological processi

  • calcium ion-regulated exocytosis of neurotransmitter Source: GO_Central
  • regulation of calcium ion-dependent exocytosis Source: UniProtKB
  • vesicle fusion Source: GO_Central

Keywordsi

Biological processExocytosis
LigandCalcium, Calcium/phospholipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Double C2-like domain-containing protein alpha
Short name:
Doc2-alpha
Gene namesi
Name:Doc2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:109446 Doc2a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Lysosome, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000799661 – 405Double C2-like domain-containing protein alphaAdd BLAST405

Proteomic databases

PaxDbiQ7TNF0
PRIDEiQ7TNF0

PTM databases

iPTMnetiQ7TNF0
PhosphoSitePlusiQ7TNF0

Expressioni

Tissue specificityi

Brain and mast cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000052301
CleanExiMM_DOC2A
ExpressionAtlasiQ7TNF0 baseline and differential
GenevisibleiQ7TNF0 MM

Interactioni

Subunit structurei

Interacts (via N-terminus) with UNC13A. Interacts with cytoplasmic dynein light chain DYNLT1 (By similarity). Interacts with UNC13D (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070119

Structurei

3D structure databases

ProteinModelPortaliQ7TNF0
SMRiQ7TNF0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini96 – 200C2 1PROSITE-ProRule annotationAdd BLAST105
Domaini258 – 361C2 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 94Interaction with UNC13D and DYNLT1By similarityAdd BLAST94
Regioni220 – 405Interaction with UNC13DBy similarityAdd BLAST186

Domaini

C2 domain 1 is involved in binding calcium and phospholipids.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410INTM Eukaryota
ENOG410YZ99 LUCA
GeneTreeiENSGT00810000125376
HOGENOMiHOG000232127
HOVERGENiHBG051388
InParanoidiQ7TNF0
KOiK19916
OMAiKVERWHT
OrthoDBiEOG091G0FPP
PhylomeDBiQ7TNF0
TreeFamiTF351844

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR014638 Doc2
IPR030535 Doc2a
IPR001565 Synaptotagmin
PANTHERiPTHR10024:SF233 PTHR10024:SF233, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
PIRSFiPIRSF036931 Doc2, 1 hit
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits

Sequencei

Sequence statusi: Complete.

Q7TNF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGRRGDRMT INIQEHMAIN VCPGPIRPIR QISDYFPRRG PGPEGGGGGG
60 70 80 90 100
GTGCGEAPAH LAPLALAPPA ALLGATTPDD GAEVDSYDSD DTTALGTLEF
110 120 130 140 150
DLLYDQASCM LHCRILRAKG LKPMDFNGLA DPYVKLHLLP GACKANKLKT
160 170 180 190 200
KTQRNTLNPV WNEELTYSGI TDDDITHKVL RISVCDEDKL SHNEFIGEIR
210 220 230 240 250
VPLRRLKPSQ KKHFNICLER QVPLPSPSSM SAALRGISCY LKELEQAEQG
260 270 280 290 300
PGLLEERGRI LLSLSYSSRR HGLLVGIVRC AHLAAMDVNG YSDPYVKTYL
310 320 330 340 350
RPDVDKKSKH KTCVKKKTLN PEFNEEFFYE IELSTLATKT LEVTVWDYDI
360 370 380 390 400
GKSNDFIGGV SLGPGARGEA QKHWNDCLHQ PDTALERWHT LTSELPPAAG

AYPLA
Length:405
Mass (Da):44,609
Last modified:October 1, 2003 - v1
Checksum:iB9EDCC03C977CCE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055768 mRNA Translation: AAH55768.1
CCDSiCCDS21847.1
RefSeqiNP_034199.1, NM_010069.1
XP_017177455.1, XM_017321966.1
XP_017177456.1, XM_017321967.1
XP_017177457.1, XM_017321968.1
XP_017177458.1, XM_017321969.1
XP_017177459.1, XM_017321970.1
XP_017177460.1, XM_017321971.1
UniGeneiMm.266301

Genome annotation databases

EnsembliENSMUST00000064110; ENSMUSP00000070119; ENSMUSG00000052301
GeneIDi13446
KEGGimmu:13446
UCSCiuc009jtb.1 mouse

Similar proteinsi

Entry informationi

Entry nameiDOC2A_MOUSE
AccessioniPrimary (citable) accession number: Q7TNF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: October 1, 2003
Last modified: March 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health