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Protein

Double C2-like domain-containing protein alpha

Gene

Doc2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium sensor which most probably regulates fusion of vesicles with membranes. Binds calcium and phospholipids. May be involved in calcium dependent neurotransmitter release through the interaction with UNC13A. May be involved in calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Regulates Ca2+-dependent secretory lysosome exocytosis in mast cells.2 Publications

GO - Molecular functioni

GO - Biological processi

  • regulation of calcium ion-dependent exocytosis Source: UniProtKB
  • synaptic vesicle exocytosis Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

Calcium, Calcium/phospholipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Double C2-like domain-containing protein alpha
Short name:
Doc2-alpha
Gene namesi
Name:Doc2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:109446. Doc2a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Lysosome, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 405405Double C2-like domain-containing protein alphaPRO_0000079966Add
BLAST

Proteomic databases

MaxQBiQ7TNF0.
PaxDbiQ7TNF0.
PRIDEiQ7TNF0.

PTM databases

iPTMnetiQ7TNF0.
PhosphoSiteiQ7TNF0.

Expressioni

Tissue specificityi

Brain and mast cells.1 Publication

Gene expression databases

BgeeiQ7TNF0.
CleanExiMM_DOC2A.
ExpressionAtlasiQ7TNF0. baseline and differential.
GenevisibleiQ7TNF0. MM.

Interactioni

Subunit structurei

Interacts (via N-terminus) with UNC13A. Interacts with cytoplasmic dynein light chain DYNLT1 (By similarity). Interacts with UNC13D (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070119.

Structurei

3D structure databases

ProteinModelPortaliQ7TNF0.
SMRiQ7TNF0. Positions 60-398.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini96 – 200105C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini258 – 361104C2 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9494Interaction with UNC13D and DYNLT1By similarityAdd
BLAST
Regioni220 – 405186Interaction with UNC13DBy similarityAdd
BLAST

Domaini

C2 domain 1 is involved in binding calcium and phospholipids.By similarity

Sequence similaritiesi

Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410INTM. Eukaryota.
ENOG410YZ99. LUCA.
GeneTreeiENSGT00810000125376.
HOGENOMiHOG000232127.
HOVERGENiHBG051388.
InParanoidiQ7TNF0.
KOiK19916.
OMAiWNEDLMY.
PhylomeDBiQ7TNF0.
TreeFamiTF351844.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR014638. Doc2.
IPR030535. Doc2a.
IPR001565. Synaptotagmin.
[Graphical view]
PANTHERiPTHR10024:SF233. PTHR10024:SF233. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PIRSFiPIRSF036931. Doc2. 1 hit.
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TNF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGRRGDRMT INIQEHMAIN VCPGPIRPIR QISDYFPRRG PGPEGGGGGG
60 70 80 90 100
GTGCGEAPAH LAPLALAPPA ALLGATTPDD GAEVDSYDSD DTTALGTLEF
110 120 130 140 150
DLLYDQASCM LHCRILRAKG LKPMDFNGLA DPYVKLHLLP GACKANKLKT
160 170 180 190 200
KTQRNTLNPV WNEELTYSGI TDDDITHKVL RISVCDEDKL SHNEFIGEIR
210 220 230 240 250
VPLRRLKPSQ KKHFNICLER QVPLPSPSSM SAALRGISCY LKELEQAEQG
260 270 280 290 300
PGLLEERGRI LLSLSYSSRR HGLLVGIVRC AHLAAMDVNG YSDPYVKTYL
310 320 330 340 350
RPDVDKKSKH KTCVKKKTLN PEFNEEFFYE IELSTLATKT LEVTVWDYDI
360 370 380 390 400
GKSNDFIGGV SLGPGARGEA QKHWNDCLHQ PDTALERWHT LTSELPPAAG

AYPLA
Length:405
Mass (Da):44,609
Last modified:October 1, 2003 - v1
Checksum:iB9EDCC03C977CCE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055768 mRNA. Translation: AAH55768.1.
CCDSiCCDS21847.1.
RefSeqiNP_034199.1. NM_010069.1.
UniGeneiMm.266301.

Genome annotation databases

EnsembliENSMUST00000064110; ENSMUSP00000070119; ENSMUSG00000052301.
GeneIDi13446.
KEGGimmu:13446.
UCSCiuc009jtb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055768 mRNA. Translation: AAH55768.1.
CCDSiCCDS21847.1.
RefSeqiNP_034199.1. NM_010069.1.
UniGeneiMm.266301.

3D structure databases

ProteinModelPortaliQ7TNF0.
SMRiQ7TNF0. Positions 60-398.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070119.

PTM databases

iPTMnetiQ7TNF0.
PhosphoSiteiQ7TNF0.

Proteomic databases

MaxQBiQ7TNF0.
PaxDbiQ7TNF0.
PRIDEiQ7TNF0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064110; ENSMUSP00000070119; ENSMUSG00000052301.
GeneIDi13446.
KEGGimmu:13446.
UCSCiuc009jtb.1. mouse.

Organism-specific databases

CTDi8448.
MGIiMGI:109446. Doc2a.

Phylogenomic databases

eggNOGiENOG410INTM. Eukaryota.
ENOG410YZ99. LUCA.
GeneTreeiENSGT00810000125376.
HOGENOMiHOG000232127.
HOVERGENiHBG051388.
InParanoidiQ7TNF0.
KOiK19916.
OMAiWNEDLMY.
PhylomeDBiQ7TNF0.
TreeFamiTF351844.

Miscellaneous databases

PROiQ7TNF0.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TNF0.
CleanExiMM_DOC2A.
ExpressionAtlasiQ7TNF0. baseline and differential.
GenevisibleiQ7TNF0. MM.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR014638. Doc2.
IPR030535. Doc2a.
IPR001565. Synaptotagmin.
[Graphical view]
PANTHERiPTHR10024:SF233. PTHR10024:SF233. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PIRSFiPIRSF036931. Doc2. 1 hit.
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "Doc2 alpha and Munc13-4 regulate Ca(2+) -dependent secretory lysosome exocytosis in mast cells."
    Higashio H., Nishimura N., Ishizaki H., Miyoshi J., Orita S., Sakane A., Sasaki T.
    J. Immunol. 180:4774-4784(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  3. Cited for: FUNCTION.

Entry informationi

Entry nameiDOC2A_MOUSE
AccessioniPrimary (citable) accession number: Q7TNF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: October 1, 2003
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.