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Protein

Glycine receptor subunit alpha-2

Gene

Glra2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Channel opening is also triggered by taurine and beta-alanine. Plays a role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Plays a role in cellular responses to ethanol.By similarity

Miscellaneous

The alpha subunit binds strychnine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi226ZincBy similarity1
Metal bindingi228ZincBy similarity1
Metal bindingi249ZincBy similarity1
Sitei295Important for obstruction of the ion pore in the closed conformationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChloride channel, Ion channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport
LigandChloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-112314 Neurotransmitter receptors and postsynaptic signal transmission

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine receptor subunit alpha-2
Gene namesi
Name:Glra2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:95748 Glra2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 256ExtracellularBy similarityAdd BLAST229
Transmembranei257 – 278Helical; Name=1By similarityAdd BLAST22
Topological domaini279 – 283CytoplasmicBy similarity5
Transmembranei284 – 304Helical; Name=2By similarityAdd BLAST21
Topological domaini305 – 315ExtracellularBy similarityAdd BLAST11
Transmembranei316 – 336Helical; Name=3By similarityAdd BLAST21
Topological domaini337 – 420CytoplasmicBy similarityAdd BLAST84
Transmembranei421 – 441Helical; Name=4By similarityAdd BLAST21
Topological domaini442 – 452ExtracellularBy similarityAdd BLAST11

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Loss of glycine-induced currents in cortical neurons from 17 dpc embryos. Still, mutant mice are born at the expected Mendelian rate, are healthy and fertile. No effect on glycine-induced currents in cortical neurons from seven day old mice (PubMed:16847326). Baseline nociception is not changed, but mutant mice show increased hyperalgesia in response to mechanical stimuli during later stages of inflammation caused by zymosan injection (PubMed:21924294).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000000041728 – 452Glycine receptor subunit alpha-2Add BLAST425

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi72N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi103N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi172 ↔ 186By similarity
Disulfide bondi232 ↔ 243By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ7TNC8
PaxDbiQ7TNC8
PRIDEiQ7TNC8

PTM databases

iPTMnetiQ7TNC8
PhosphoSitePlusiQ7TNC8
SwissPalmiQ7TNC8

Expressioni

Tissue specificityi

Detected in the retina inner plexiform layer (at protein level) (PubMed:15329889). Detected in neonate retina. Detected in brain (PubMed:21924294). Detected in spinal cord, with higher levels in the dorsal horn (PubMed:16847326, PubMed:21924294).3 Publications

Gene expression databases

BgeeiENSMUSG00000018589
CleanExiMM_GLRA2
ExpressionAtlasiQ7TNC8 baseline and differential
GenevisibleiQ7TNC8 MM

Interactioni

Subunit structurei

Homopentamer (in vitro). Interacts with GLRB. Heteropentamer composed of GLRA2 and GLRB. Both homopentamers and heteropentamers form functional ion channels, but their characteristics are subtly different.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060827

Structurei

3D structure databases

ProteinModelPortaliQ7TNC8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni236 – 241Strychnine-bindingBy similarity6

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3643 Eukaryota
ENOG410XPWH LUCA
GeneTreeiENSGT00760000118821
HOGENOMiHOG000231336
HOVERGENiHBG051707
InParanoidiQ7TNC8
KOiK05194
OMAiFRTTFCK
OrthoDBiEOG091G0805
PhylomeDBiQ7TNC8
TreeFamiTF315453

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006028 GABAA/Glycine_rcpt
IPR008127 Glycine_rcpt_A
IPR008129 Glycine_rcpt_A2
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
PANTHERiPTHR18945 PTHR18945, 1 hit
PTHR18945:SF28 PTHR18945:SF28, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00253 GABAARECEPTR
PR01673 GLYRALPHA
PR01675 GLYRALPHA2
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7TNC8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRQLVNILT ALFAFFLGTN HFREAFCKDH DSRSGKHPSQ TLSPSDFLDK
60 70 80 90 100
LMGRTSGYDA RIRPNFKGPP VNVTCNIFIN SFGSVTETTM DYRVNIFLRQ
110 120 130 140 150
QWNDSRLAYS EYPDDSLDLD PSMLDSIWKP DLFFANEKGA NFHDVTTDNK
160 170 180 190 200
LLRISKNGKV LYSIRLTLTL SCPMDLKNFP MDVQTCTMQL ESFGYTMNDL
210 220 230 240 250
IFEWLSDGPV QVAEGLTLPQ FILKEEKELG YCTKHYNTGK FTCIEVKFHL
260 270 280 290 300
ERQMGYYLIQ MYIPSLLIVI LSWVSFWINM DAAPARVALG ITTVLTMTTQ
310 320 330 340 350
SSGSRASLPK VSYVKAIDIW MAVCLLFVFA ALLEYAAVNF VSRQHKEFLR
360 370 380 390 400
LRRRQKRQNK EEDVTRESRF NFSGYGMGHC LQMKDGTAVK ATPANPLPQP
410 420 430 440 450
PKDADAIKKK FVDRAKRIDT ISRAAFPLAF LIFNIFYWIT YKIIRHEDVH

KK
Length:452
Mass (Da):52,062
Last modified:October 1, 2003 - v1
Checksum:iC8962DFD7D6D8A61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056342 mRNA Translation: AAH56342.1
BC068987 mRNA Translation: AAH68987.1
CCDSiCCDS41206.1
RefSeqiNP_906272.1, NM_183427.4
XP_006528897.1, XM_006528834.1
XP_006528898.1, XM_006528835.3
UniGeneiMm.113877

Genome annotation databases

EnsembliENSMUST00000058787; ENSMUSP00000060827; ENSMUSG00000018589
GeneIDi237213
KEGGimmu:237213
UCSCiuc009uwb.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGLRA2_MOUSE
AccessioniPrimary (citable) accession number: Q7TNC8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: October 1, 2003
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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