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Protein

Glycine receptor subunit alpha-2

Gene

Glra2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Channel opening is also triggered by taurine and beta-alanine. Plays a role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Plays a role in cellular responses to ethanol.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi226ZincBy similarity1
Metal bindingi228ZincBy similarity1
Metal bindingi249ZincBy similarity1
Sitei295Important for obstruction of the ion pore in the closed conformationBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-975298. Ligand-gated ion channel transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine receptor subunit alpha-2
Gene namesi
Name:Glra2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:95748. Glra2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 256ExtracellularBy similarityAdd BLAST229
Transmembranei257 – 278Helical; Name=1By similarityAdd BLAST22
Topological domaini279 – 283CytoplasmicBy similarity5
Transmembranei284 – 304Helical; Name=2By similarityAdd BLAST21
Topological domaini305 – 315ExtracellularBy similarityAdd BLAST11
Transmembranei316 – 336Helical; Name=3By similarityAdd BLAST21
Topological domaini337 – 420CytoplasmicBy similarityAdd BLAST84
Transmembranei421 – 441Helical; Name=4By similarityAdd BLAST21
Topological domaini442 – 452ExtracellularBy similarityAdd BLAST11

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Loss of glycine-induced currents in cortical neurons from 17 dpc embryos. Still, mutant mice are born at the expected Mendelian rate, are healthy and fertile. No effect on glycine-induced currents in cortical neurons from seven day old mice (PubMed:16847326). Baseline nociception is not changed, but mutant mice show increased hyperalgesia in response to mechanical stimuli during later stages of inflammation caused by zymosan injection (PubMed:21924294).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000000041728 – 452Glycine receptor subunit alpha-2Add BLAST425

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi72N-linked (GlcNAc...)Sequence analysis1
Glycosylationi103N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi172 ↔ 186By similarity
Disulfide bondi232 ↔ 243By similarity
Modified residuei368PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ7TNC8.
PaxDbiQ7TNC8.
PRIDEiQ7TNC8.

PTM databases

iPTMnetiQ7TNC8.
PhosphoSitePlusiQ7TNC8.

Expressioni

Tissue specificityi

Detected in the retina inner plexiform layer (at protein level) (PubMed:15329889). Detected in neonate retina. Detected in brain (PubMed:21924294). Detected in spinal cord, with higher levels in the dorsal horn (PubMed:16847326, PubMed:21924294).3 Publications

Gene expression databases

BgeeiENSMUSG00000018589.
CleanExiMM_GLRA2.
ExpressionAtlasiQ7TNC8. baseline and differential.
GenevisibleiQ7TNC8. MM.

Interactioni

Subunit structurei

Homopentamer (in vitro). Interacts with GLRB. Heteropentamer composed of GLRA2 and GLRB. Both homopentamers and heteropentamers form functional ion channels, but their characteristics are subtly different.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060827.

Structurei

3D structure databases

ProteinModelPortaliQ7TNC8.
SMRiQ7TNC8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni236 – 241Strychnine-bindingBy similarity6

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiQ7TNC8.
KOiK05194.
OMAiFREAFCK.
OrthoDBiEOG091G0805.
PhylomeDBiQ7TNC8.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR008129. Glycine_rcpt_A2.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR01675. GLYRALPHA2.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7TNC8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRQLVNILT ALFAFFLGTN HFREAFCKDH DSRSGKHPSQ TLSPSDFLDK
60 70 80 90 100
LMGRTSGYDA RIRPNFKGPP VNVTCNIFIN SFGSVTETTM DYRVNIFLRQ
110 120 130 140 150
QWNDSRLAYS EYPDDSLDLD PSMLDSIWKP DLFFANEKGA NFHDVTTDNK
160 170 180 190 200
LLRISKNGKV LYSIRLTLTL SCPMDLKNFP MDVQTCTMQL ESFGYTMNDL
210 220 230 240 250
IFEWLSDGPV QVAEGLTLPQ FILKEEKELG YCTKHYNTGK FTCIEVKFHL
260 270 280 290 300
ERQMGYYLIQ MYIPSLLIVI LSWVSFWINM DAAPARVALG ITTVLTMTTQ
310 320 330 340 350
SSGSRASLPK VSYVKAIDIW MAVCLLFVFA ALLEYAAVNF VSRQHKEFLR
360 370 380 390 400
LRRRQKRQNK EEDVTRESRF NFSGYGMGHC LQMKDGTAVK ATPANPLPQP
410 420 430 440 450
PKDADAIKKK FVDRAKRIDT ISRAAFPLAF LIFNIFYWIT YKIIRHEDVH

KK
Length:452
Mass (Da):52,062
Last modified:October 1, 2003 - v1
Checksum:iC8962DFD7D6D8A61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056342 mRNA. Translation: AAH56342.1.
BC068987 mRNA. Translation: AAH68987.1.
CCDSiCCDS41206.1.
RefSeqiNP_906272.1. NM_183427.4.
XP_006528897.1. XM_006528834.1.
XP_006528898.1. XM_006528835.3.
UniGeneiMm.113877.

Genome annotation databases

EnsembliENSMUST00000058787; ENSMUSP00000060827; ENSMUSG00000018589.
GeneIDi237213.
KEGGimmu:237213.
UCSCiuc009uwb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056342 mRNA. Translation: AAH56342.1.
BC068987 mRNA. Translation: AAH68987.1.
CCDSiCCDS41206.1.
RefSeqiNP_906272.1. NM_183427.4.
XP_006528897.1. XM_006528834.1.
XP_006528898.1. XM_006528835.3.
UniGeneiMm.113877.

3D structure databases

ProteinModelPortaliQ7TNC8.
SMRiQ7TNC8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060827.

PTM databases

iPTMnetiQ7TNC8.
PhosphoSitePlusiQ7TNC8.

Proteomic databases

MaxQBiQ7TNC8.
PaxDbiQ7TNC8.
PRIDEiQ7TNC8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058787; ENSMUSP00000060827; ENSMUSG00000018589.
GeneIDi237213.
KEGGimmu:237213.
UCSCiuc009uwb.1. mouse.

Organism-specific databases

CTDi2742.
MGIiMGI:95748. Glra2.

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiQ7TNC8.
KOiK05194.
OMAiFREAFCK.
OrthoDBiEOG091G0805.
PhylomeDBiQ7TNC8.
TreeFamiTF315453.

Enzyme and pathway databases

ReactomeiR-MMU-975298. Ligand-gated ion channel transport.

Miscellaneous databases

PROiQ7TNC8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018589.
CleanExiMM_GLRA2.
ExpressionAtlasiQ7TNC8. baseline and differential.
GenevisibleiQ7TNC8. MM.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR008129. Glycine_rcpt_A2.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR01675. GLYRALPHA2.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLRA2_MOUSE
AccessioniPrimary (citable) accession number: Q7TNC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: October 1, 2003
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The alpha subunit binds strychnine.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.