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Protein

Glycine receptor subunit alpha-2

Gene

Glra2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Channel opening is also triggered by taurine and beta-alanine. Plays a role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Plays a role in cellular responses to ethanol.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi226 – 2261ZincBy similarity
Metal bindingi228 – 2281ZincBy similarity
Metal bindingi249 – 2491ZincBy similarity
Sitei295 – 2951Important for obstruction of the ion pore in the closed conformationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-975298. Ligand-gated ion channel transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine receptor subunit alpha-2
Gene namesi
Name:Glra2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:95748. Glra2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 256229ExtracellularBy similarityAdd
BLAST
Transmembranei257 – 27822Helical; Name=1By similarityAdd
BLAST
Topological domaini279 – 2835CytoplasmicBy similarity
Transmembranei284 – 30421Helical; Name=2By similarityAdd
BLAST
Topological domaini305 – 31511ExtracellularBy similarityAdd
BLAST
Transmembranei316 – 33621Helical; Name=3By similarityAdd
BLAST
Topological domaini337 – 42084CytoplasmicBy similarityAdd
BLAST
Transmembranei421 – 44121Helical; Name=4By similarityAdd
BLAST
Topological domaini442 – 45211ExtracellularBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Loss of glycine-induced currents in cortical neurons from 17 dpc embryos. Still, mutant mice are born at the expected Mendelian rate, are healthy and fertile. No effect on glycine-induced currents in cortical neurons from seven day old mice (PubMed:16847326). Baseline nociception is not changed, but mutant mice show increased hyperalgesia in response to mechanical stimuli during later stages of inflammation caused by zymosan injection (PubMed:21924294).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Chaini28 – 452425Glycine receptor subunit alpha-2PRO_0000000417Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence analysis
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence analysis
Disulfide bondi172 ↔ 186By similarity
Disulfide bondi232 ↔ 243By similarity
Modified residuei368 – 3681PhosphoserineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ7TNC8.
PaxDbiQ7TNC8.
PRIDEiQ7TNC8.

PTM databases

iPTMnetiQ7TNC8.
PhosphoSiteiQ7TNC8.

Expressioni

Tissue specificityi

Detected in the retina inner plexiform layer (at protein level) (PubMed:15329889). Detected in neonate retina. Detected in brain (PubMed:21924294). Detected in spinal cord, with higher levels in the dorsal horn (PubMed:16847326, PubMed:21924294).3 Publications

Gene expression databases

BgeeiENSMUSG00000018589.
CleanExiMM_GLRA2.
ExpressionAtlasiQ7TNC8. baseline and differential.
GenevisibleiQ7TNC8. MM.

Interactioni

Subunit structurei

Homopentamer (in vitro). Interacts with GLRB. Heteropentamer composed of GLRA2 and GLRB. Both homopentamers and heteropentamers form functional ion channels, but their characteristics are subtly different.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060827.

Structurei

3D structure databases

ProteinModelPortaliQ7TNC8.
SMRiQ7TNC8. Positions 48-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni236 – 2416Strychnine-bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiQ7TNC8.
KOiK05194.
OMAiFREAFCK.
OrthoDBiEOG091G0805.
PhylomeDBiQ7TNC8.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR008129. Glycine_rcpt_A2.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR01675. GLYRALPHA2.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7TNC8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRQLVNILT ALFAFFLGTN HFREAFCKDH DSRSGKHPSQ TLSPSDFLDK
60 70 80 90 100
LMGRTSGYDA RIRPNFKGPP VNVTCNIFIN SFGSVTETTM DYRVNIFLRQ
110 120 130 140 150
QWNDSRLAYS EYPDDSLDLD PSMLDSIWKP DLFFANEKGA NFHDVTTDNK
160 170 180 190 200
LLRISKNGKV LYSIRLTLTL SCPMDLKNFP MDVQTCTMQL ESFGYTMNDL
210 220 230 240 250
IFEWLSDGPV QVAEGLTLPQ FILKEEKELG YCTKHYNTGK FTCIEVKFHL
260 270 280 290 300
ERQMGYYLIQ MYIPSLLIVI LSWVSFWINM DAAPARVALG ITTVLTMTTQ
310 320 330 340 350
SSGSRASLPK VSYVKAIDIW MAVCLLFVFA ALLEYAAVNF VSRQHKEFLR
360 370 380 390 400
LRRRQKRQNK EEDVTRESRF NFSGYGMGHC LQMKDGTAVK ATPANPLPQP
410 420 430 440 450
PKDADAIKKK FVDRAKRIDT ISRAAFPLAF LIFNIFYWIT YKIIRHEDVH

KK
Length:452
Mass (Da):52,062
Last modified:October 1, 2003 - v1
Checksum:iC8962DFD7D6D8A61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056342 mRNA. Translation: AAH56342.1.
BC068987 mRNA. Translation: AAH68987.1.
CCDSiCCDS41206.1.
RefSeqiNP_906272.1. NM_183427.4.
XP_006528897.1. XM_006528834.1.
XP_006528898.1. XM_006528835.2.
UniGeneiMm.113877.

Genome annotation databases

EnsembliENSMUST00000058787; ENSMUSP00000060827; ENSMUSG00000018589.
GeneIDi237213.
KEGGimmu:237213.
UCSCiuc009uwb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056342 mRNA. Translation: AAH56342.1.
BC068987 mRNA. Translation: AAH68987.1.
CCDSiCCDS41206.1.
RefSeqiNP_906272.1. NM_183427.4.
XP_006528897.1. XM_006528834.1.
XP_006528898.1. XM_006528835.2.
UniGeneiMm.113877.

3D structure databases

ProteinModelPortaliQ7TNC8.
SMRiQ7TNC8. Positions 48-448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060827.

PTM databases

iPTMnetiQ7TNC8.
PhosphoSiteiQ7TNC8.

Proteomic databases

MaxQBiQ7TNC8.
PaxDbiQ7TNC8.
PRIDEiQ7TNC8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058787; ENSMUSP00000060827; ENSMUSG00000018589.
GeneIDi237213.
KEGGimmu:237213.
UCSCiuc009uwb.1. mouse.

Organism-specific databases

CTDi2742.
MGIiMGI:95748. Glra2.

Phylogenomic databases

eggNOGiKOG3643. Eukaryota.
ENOG410XPWH. LUCA.
GeneTreeiENSGT00760000118821.
HOGENOMiHOG000231336.
HOVERGENiHBG051707.
InParanoidiQ7TNC8.
KOiK05194.
OMAiFREAFCK.
OrthoDBiEOG091G0805.
PhylomeDBiQ7TNC8.
TreeFamiTF315453.

Enzyme and pathway databases

ReactomeiR-MMU-975298. Ligand-gated ion channel transport.

Miscellaneous databases

PROiQ7TNC8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018589.
CleanExiMM_GLRA2.
ExpressionAtlasiQ7TNC8. baseline and differential.
GenevisibleiQ7TNC8. MM.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR008127. Glycine_rcpt_A.
IPR008129. Glycine_rcpt_A2.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01673. GLYRALPHA.
PR01675. GLYRALPHA2.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLRA2_MOUSE
AccessioniPrimary (citable) accession number: Q7TNC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: October 1, 2003
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The alpha subunit binds strychnine.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.