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Protein

Kinesin-like protein KIF26B

Gene

Kif26b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for embryonic kidney development. Plays an important role in the compact adhesion between mesenchymal cells adjacent to the ureteric buds, possibly by interacting with MYH10. This could lead to the establishment of the basolateral integrity of the mesenchyme and the polarized expression of ITGA8, which maintains the GDNF expression required for further ureteric bud attraction. Although it seems to lack ATPase activity it is constitutively associated with microtubules.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi546 – 5538ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • establishment of cell polarity Source: MGI
  • positive regulation of cell-cell adhesion Source: MGI
  • ureteric bud invasion Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Motor protein

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF26B
Gene namesi
Name:Kif26b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2447076. Kif26b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Mutant mice die within 24 hours after birth. Most mice show bilateral kidney agenesis. The development of other organs seems normal. Ureteric bud attraction is impaired after 11.0 dpc. The ureteric buds are attracted close to the mesenchyme but fail to invade and branch into the mesenchyme and the kidney disappears by 14.5 dpc. The mesenchymal cells undergo apoptotic cell death at 12.5 dpc. ITGA8 is reduced at the ureteric bud/mesenchyme junction and does not exhibit basolateral localization. GDNF is not properly maintained in the mesenchyme at 11.5 dpc.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1859 – 18591T → A: Reduced polyubiquitination when co-expressed with NEDD4; when associated with Ala-1962. 1 Publication
Mutagenesisi1962 – 19621S → A: Reduced polyubiquitination when co-expressed with NEDD4; when associated with Ala-1859. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21122112Kinesin-like protein KIF26BPRO_0000307302Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1859 – 18591Phosphothreonine1 Publication
Modified residuei1962 – 19621Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation at Thr-1859 and Ser-1962 by CDKs, mainly CDK2 and CDK5, enhances the interaction with NEDD4, polyubiquitination, and subsequent proteasomal degradation. Phosphorylation occurs upon loss of interaction with microtubules.1 Publication
Polyubiquitinated by NEDD4, resulting in proteasomal degradation.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ7TNC6.
PaxDbiQ7TNC6.
PRIDEiQ7TNC6.

PTM databases

iPTMnetiQ7TNC6.
PhosphoSiteiQ7TNC6.

Expressioni

Tissue specificityi

In newborn kidney, specifically expressed in the mesenchyme.1 Publication

Developmental stagei

In the developing kidney, only present in the uncommitted mesenchyme and absent from more differentiated structures including renal vesicles and comma-shaped bodies. First detected in the metanephric mesenchyme at 10.5 dpc. After 11.5 dpc, observed in mesenchymal cells surrounding the tips of ureteric buds in the metanephroi. At 11.5 dpc, also detected in limb buds and central nervous system. At 14.5 dpc, strongly expressed in the nephrogenic zone.

Inductioni

Up-regulated by SALL1.1 Publication

Gene expression databases

BgeeiQ7TNC6.
CleanExiMM_KIF26B.
ExpressionAtlasiQ7TNC6. baseline and differential.
GenevisibleiQ7TNC6. MM.

Interactioni

Subunit structurei

Interacts with MYH10.1 Publication

Protein-protein interaction databases

DIPiDIP-59327N.
STRINGi10090.ENSMUSP00000124462.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini450 – 801352Kinesin motorPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi58 – 12669Gly-richAdd
BLAST
Compositional biasi1015 – 1119105Ser-richAdd
BLAST
Compositional biasi1648 – 1947300Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIF26 subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4280. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00650000093360.
HOGENOMiHOG000081802.
HOVERGENiHBG108063.
InParanoidiQ7TNC6.
KOiK10404.
OMAiDHRNSHM.
OrthoDBiEOG7J445V.
PhylomeDBiQ7TNC6.
TreeFamiTF105235.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TNC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSVAGNKER LAVSTRGKKY GVNEMCSPTK PSAPCSPESW YRKAYEESRA
60 70 80 90 100
GSRPTPEGAG SALGSSGTPS PGSGTSSPSS FTGSPGPASP GIGTSSPGSL
110 120 130 140 150
GGSPGFGTGS PGSGSGGGSS PGSDRGVWCE NCNARLVELK RQALKLLLPG
160 170 180 190 200
PLPGKDPAFS AVIHDKLQVP NTIRKSWNDR DNRCDICATH LNQLKQEAIQ
210 220 230 240 250
MVLTLEQAAG SEHYDTSLGS PPPISSIPTL VGSRHMGGLQ QPREWAFVPA
260 270 280 290 300
PYATSTYTGL VNKHSGKPNS LGVSNGAEKK SGSPTHQAKV SLQMATSPSN
310 320 330 340 350
GNILNSVAIQ AHQYLDGTWS LSRTNGVTLY PYQISQLMTE TGREGLTESA
360 370 380 390 400
LNRYNADKPA ASSVPAPQGS CVASETSTGT SVAASFFARA AQKLNLSSKK
410 420 430 440 450
KKHRPSTPSV AEAPLFATSF SGILQTSPPP APPCLLRAVN KVKDTPGMGK
460 470 480 490 500
VKVMLRICST SARDTSESSS FLKVDPRKKQ ITLYDPLTCG GQNAFQKRSS
510 520 530 540 550
QVPPKMFAFD AVFPQDASQA EVCAGTVAEV IQSVVNGADG CVFCFGHAKL
560 570 580 590 600
GKSYTMIGRD DSMQNLGIIP CAISWLFKLI NERKEKTGAR FSVRISAVEV
610 620 630 640 650
WGKEENLRDL LSEVATGSLQ DGQSPGVYLC EDPICGTQLQ NQSELRAPTA
660 670 680 690 700
EKAAFFLDAA IASRRSNQQD CDEDDHRNSH MLFTLHIYQY RMEKSGKGGM
710 720 730 740 750
SGGRSRLHLI DLGSCVKALS KNREGGSGLC LSLSALGNVI LALVNGSKHI
760 770 780 790 800
PYKESKLTML LRESLGNVNC RTTMIAHISA AASSYAETLS TIQIASRVLR
810 820 830 840 850
MKKKKTKYTS SSSGGESSCE EGRMRRPTQL RPFHARAPVD PEFPLAPLSS
860 870 880 890 900
DPDYSSSSEQ SCDTVIYIGP NGTALSDKEL TDNEGPPDFV PIVPALQKTR
910 920 930 940 950
GDSRPGEAAE AAASKSERDC LKCNTFAELQ ERLDCIDGSE EPSKFPFEEL
960 970 980 990 1000
PIQFGPEQAG RCAPLSQAVG PDTPSESEKE DNGSDNGQLT EREGTELPAS
1010 1020 1030 1040 1050
KAQRNRSPAP TVTRSSSPSP ASPRSIPGSS SQHSTSQLTQ SPSLQSSRES
1060 1070 1080 1090 1100
LNSCGFVEGK PRPMGSPRLG IASLSKTSEY KPPSSPSQRC KVYTQKGVLP
1110 1120 1130 1140 1150
SSAPPPSLSK DSGMVSSESL LQPDVRTPPV GMSPQVLKKS MSAGSEGFPG
1160 1170 1180 1190 1200
TLVDGEDQEG PPADSKKEIL STTMVTVQQP LELNGEDELV FTLVEELTIS
1210 1220 1230 1240 1250
GVLDSGRPTS IISFNSDCSV QALASGSRPV SIISSISEDL ECYSSMAPVS
1260 1270 1280 1290 1300
EVSITQFLPL PKLGLDEKAR DAGSRRSSIS SWLSEMSAGS DGEQSCHSFI
1310 1320 1330 1340 1350
AQTCFGHGEA MAEPPASEFV SSIQNTAVVC REKPEVGPDN LLILSEMGEE
1360 1370 1380 1390 1400
SGNKAAPIKG CKISTVGKAM VTISNTASLS SCEGYIPMKT NITVYPCIAM
1410 1420 1430 1440 1450
SPRNVQEPES STATPKVSPK ASQAQESKEP STRREMKFED PWLKREEEVK
1460 1470 1480 1490 1500
RENAYSSEEG VKCEPLPSSL KTEGKSEQEL DGRPSSGNRL SSSSSEAAAF
1510 1520 1530 1540 1550
QGTDNVRRVV DGCEMALPGL VAQSPLHVNR NLKSSSLPRA FQKADRHEEL
1560 1570 1580 1590 1600
DSFYHCLADS NGFSAASGIP SSKTTLERKV ASPKHCVLAR PKGTPPLPPV
1610 1620 1630 1640 1650
RKSSLDQKNR ASPQHGGGSS NTSSPLNQPA TFLACFPDES NGKTKDVSSS
1660 1670 1680 1690 1700
SKLFSAKLEQ LASRSNSLGR TTVSHYECLS LERAESLSSV SSRMHAGKDS
1710 1720 1730 1740 1750
TMPRTGRSLG RSTGASPPSC GITQSTGASP KASQSKISAV SKLLLASPKS
1760 1770 1780 1790 1800
RSSLSTSTTK TLSFSTKSLP QSVGQSSNLP PSGKHMSWST QSLSRNRGSG
1810 1820 1830 1840 1850
LASKLPLRAV NGRISELLQG SAGPRSAQLR AEAEERSGAP SEDKPAAAHL
1860 1870 1880 1890 1900
LPSPYSKITP PRKPHRCSSG HGSDNSSVLS GELPPAMGKT ALFYHSGGSS
1910 1920 1930 1940 1950
GYESMMRDSE ATGSASSAQD SMSENSSSVG GRCRSLKNQK KRSNSGSQRR
1960 1970 1980 1990 2000
RLIPALSLDT PSPVRKTASS TGVRWVDGPL RSTQRSLGEP FEIKVYEIDD
2010 2020 2030 2040 2050
VERLQRRRGA TSKEVMCFNA KLKILEHRQQ RIAEVRAKYE WLMKELEATK
2060 2070 2080 2090 2100
QYLMLDPNKW LREFDLEQVL QLDSLEYLEA LEGVTERLES RVNFCKAHLM
2110
MITCFDITSR RR
Length:2,112
Mass (Da):225,511
Last modified:July 27, 2011 - v3
Checksum:iACAC968F636DC4D6
GO

Sequence cautioni

The sequence AAH56349.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC36343.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti870 – 8701P → L in AAH56349 (PubMed:15489334).Curated
Sequence conflicti1547 – 15471H → R in BAC36343 (PubMed:16141072).Curated
Sequence conflicti2050 – 20501K → R in BAJ06134 (PubMed:20439720).Curated
Sequence conflicti2050 – 20501K → R in AAH56349 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB355846 mRNA. Translation: BAJ06134.1.
AC117695 Genomic DNA. No translation available.
AC117756 Genomic DNA. No translation available.
AC117766 Genomic DNA. No translation available.
AC140776 Genomic DNA. No translation available.
BC056349 mRNA. Translation: AAH56349.1. Different initiation.
AK076435 mRNA. Translation: BAC36343.1. Different initiation.
CCDSiCCDS48464.1.
RefSeqiNP_001155137.1. NM_001161665.1.
XP_011237124.1. XM_011238822.1.
UniGeneiMm.129727.
Mm.138659.

Genome annotation databases

EnsembliENSMUST00000161017; ENSMUSP00000124462; ENSMUSG00000026494.
GeneIDi269152.
KEGGimmu:269152.
UCSCiuc007dvh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB355846 mRNA. Translation: BAJ06134.1.
AC117695 Genomic DNA. No translation available.
AC117756 Genomic DNA. No translation available.
AC117766 Genomic DNA. No translation available.
AC140776 Genomic DNA. No translation available.
BC056349 mRNA. Translation: AAH56349.1. Different initiation.
AK076435 mRNA. Translation: BAC36343.1. Different initiation.
CCDSiCCDS48464.1.
RefSeqiNP_001155137.1. NM_001161665.1.
XP_011237124.1. XM_011238822.1.
UniGeneiMm.129727.
Mm.138659.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59327N.
STRINGi10090.ENSMUSP00000124462.

PTM databases

iPTMnetiQ7TNC6.
PhosphoSiteiQ7TNC6.

Proteomic databases

MaxQBiQ7TNC6.
PaxDbiQ7TNC6.
PRIDEiQ7TNC6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000161017; ENSMUSP00000124462; ENSMUSG00000026494.
GeneIDi269152.
KEGGimmu:269152.
UCSCiuc007dvh.2. mouse.

Organism-specific databases

CTDi55083.
MGIiMGI:2447076. Kif26b.

Phylogenomic databases

eggNOGiKOG4280. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00650000093360.
HOGENOMiHOG000081802.
HOVERGENiHBG108063.
InParanoidiQ7TNC6.
KOiK10404.
OMAiDHRNSHM.
OrthoDBiEOG7J445V.
PhylomeDBiQ7TNC6.
TreeFamiTF105235.

Miscellaneous databases

PROiQ7TNC6.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TNC6.
CleanExiMM_KIF26B.
ExpressionAtlasiQ7TNC6. baseline and differential.
GenevisibleiQ7TNC6. MM.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 2 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH MYH10, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INDUCTION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 494-2112.
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1274-2112.
    Strain: C57BL/6J.
    Tissue: Head.
  5. "Phosphorylation of Kif26b promotes its polyubiquitination and subsequent proteasomal degradation during kidney development."
    Terabayashi T., Sakaguchi M., Shinmyozu K., Ohshima T., Johjima A., Ogura T., Miki H., Nishinakamura R.
    PLoS ONE 7:E39714-E39714(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-1859 AND SER-1962, MUTAGENESIS OF THR-1859 AND SER-1962, FUNCTION.

Entry informationi

Entry nameiKI26B_MOUSE
AccessioniPrimary (citable) accession number: Q7TNC6
Secondary accession number(s): D5MP63
, E9PXV6, Q0VB48, Q8BHX2, Q8BKH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.