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Protein

Cytoplasmic polyadenylation element-binding protein 3

Gene

Cpeb3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • mRNA 3'-UTR AU-rich region binding Source: UniProtKB
  • mRNA 3'-UTR binding Source: UniProtKB
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: MGI
  • RNA binding Source: MGI
  • RNA stem-loop binding Source: UniProtKB
  • translation factor activity, RNA binding Source: UniProtKB
  • translation repressor activity, nucleic acid binding Source: UniProtKB

GO - Biological processi

  • 3'-UTR-mediated mRNA destabilization Source: UniProtKB
  • cellular response to amino acid stimulus Source: UniProtKB
  • long-term memory Source: UniProtKB
  • negative regulation of cytoplasmic translational elongation Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • negative regulation of translation Source: UniProtKB
  • positive regulation of dendritic spine development Source: UniProtKB
  • positive regulation of mRNA polyadenylation Source: UniProtKB
  • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: UniProtKB
  • positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: UniProtKB
  • positive regulation of translation Source: UniProtKB
  • regulation of dendritic spine development Source: UniProtKB
  • regulation of synaptic plasticity Source: UniProtKB
  • translation Source: MGI
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic polyadenylation element-binding protein 3
Short name:
CPE-BP3
Short name:
CPE-binding protein 3
Short name:
mCPEB-3
Gene namesi
Name:Cpeb3
Synonyms:Kiaa0940
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2443075. Cpeb3.

Subcellular locationi

GO - Cellular componenti

  • apical dendrite Source: UniProtKB
  • CCR4-NOT complex Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • dendrite Source: UniProtKB
  • neuron projection Source: UniProtKB
  • nucleus Source: UniProtKB
  • synapse Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Cytoplasmic polyadenylation element-binding protein 3PRO_0000269262Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei194 – 1941PhosphoserineCombined sources
Modified residuei197 – 1971PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ7TN99.
PaxDbiQ7TN99.
PRIDEiQ7TN99.

PTM databases

iPTMnetiQ7TN99.
PhosphoSiteiQ7TN99.

Expressioni

Tissue specificityi

Highly expressed in brain and heart, less in liver, kidney, embryo, skeletal muscle, lung and ovary. Weakly expressed in granular cells of dentate gyrus and the pyramidal cells of CA3 and CA1 of the hippocampus.1 Publication

Inductioni

Up-regulated in granular cells of the dentate gyrus and the pyramidal cells of CA1 and CA3 after kainate-induced seizures.1 Publication

Gene expression databases

BgeeiQ7TN99.
ExpressionAtlasiQ7TN99. baseline and differential.
GenevisibleiQ7TN99. MM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Neurl1Q923S69EBI-5376779,EBI-5376802
UbcP629912EBI-5376779,EBI-413074

Protein-protein interaction databases

BioGridi229024. 1 interaction.
IntActiQ7TN99. 2 interactions.
STRINGi10090.ENSMUSP00000078690.

Structurei

3D structure databases

ProteinModelPortaliQ7TN99.
SMRiQ7TN99. Positions 449-707.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini459 – 55092RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini567 – 64983RRM 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi13 – 3119Gln-richAdd
BLAST
Compositional biasi32 – 202171Pro-richAdd
BLAST
Compositional biasi226 – 23611Poly-AlaAdd
BLAST

Sequence similaritiesi

Belongs to the RRM CPEB family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0129. Eukaryota.
ENOG410Y1XZ. LUCA.
GeneTreeiENSGT00390000012886.
HOGENOMiHOG000290660.
HOVERGENiHBG058010.
InParanoidiQ7TN99.
KOiK02602.
OrthoDBiEOG7X3QR2.
PhylomeDBiQ7TN99.
TreeFamiTF317658.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR032296. CEBP_ZZ.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF16366. CEBP_ZZ. 1 hit.
PF16367. RRM_7. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TN99-1) [UniParc]FASTAAdd to basket

Also known as: mCPEB-3a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQDDLLMDKS KTQPQSQQQQ RQQQQQQQQL QPEPGAAEAP STPLSSEIPK
60 70 80 90 100
PEDSSAVPAL SPASAPPAPN GPDKMQMESP LLPGLSFHQP PQQPPPPQEP
110 120 130 140 150
TAPGASLSPS FGSTWSTGTT NAVEDSFFQG ITPVNGTMLF QNFPHHVNPV
160 170 180 190 200
FGGTFSPQIG LAQTQHHQQP PPPAPQPPQP AQPPQAQPSQ QRRSPASPSQ
210 220 230 240 250
APYAQRSAAA YGHQPIMTSK PSSSSAVAAA AAAAAASSAS SSWNTHQSVN
260 270 280 290 300
AAWSAPSNPW GGLQAGRDPR RAVGVGVGVG VGVPSPLNPI SPLKKPFSSN
310 320 330 340 350
VIAPPKFPRA APLTSKSWME DNAFRTDNGN NLLPFQDRSR PYDTFNLHSL
360 370 380 390 400
ENSLMDMIRT DHEPLKGKHY PNSGPPMSFA DIMWRNHFAG RMGINFHHPG
410 420 430 440 450
TDNIMALNTR SYGRRRGRSS LFPFEDAFLD DSHGDQALSS GLSSPTRCQN
460 470 480 490 500
GERVERYSRK VFVGGLPPDI DEDEITASFR RFGPLVVDWP HKAESKSYFP
510 520 530 540 550
PKGYAFLLFQ EESSVQALID ACLEEDGKLY LCVSSPTIKD KPVQIRPWNL
560 570 580 590 600
SDSDFVMDGS QPLDPRKTIF VGGVPRPLRA VELAMIMDRL YGGVCYAGID
610 620 630 640 650
TDPELKYPKG AGRVAFSNQQ SYIAAISARF VQLQHNDIDK RVEVKPYVLD
660 670 680 690 700
DQMCDECQGT RCGGKFAPFF CANVTCLQYY CEYCWASIHS RAGREFHKPL
710
VKEGGDRPRH VPFRWS
Length:716
Mass (Da):78,335
Last modified:October 1, 2003 - v1
Checksum:i1FA1D8125FD69819
GO
Isoform 2 (identifier: Q7TN99-2) [UniParc]FASTAAdd to basket

Also known as: mCPEB-3b

The sequence of this isoform differs from the canonical sequence as follows:
     409-416: Missing.

Show »
Length:708
Mass (Da):77,302
Checksum:i44921082F8A1AEAC
GO
Isoform 3 (identifier: Q7TN99-3) [UniParc]FASTAAdd to basket

Also known as: mCPEB-3c

The sequence of this isoform differs from the canonical sequence as follows:
     367-389: Missing.

Show »
Length:693
Mass (Da):75,692
Checksum:i8DF1697A5BAEF6AF
GO
Isoform 4 (identifier: Q7TN99-4) [UniParc]FASTAAdd to basket

Also known as: mCPEB-3d

The sequence of this isoform differs from the canonical sequence as follows:
     367-389: Missing.
     409-416: Missing.

Show »
Length:685
Mass (Da):74,659
Checksum:i088EA7D992502AFD
GO
Isoform 5 (identifier: Q7TN99-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     367-389: Missing.
     581-584: VELA → GEWK
     585-716: Missing.

Show »
Length:561
Mass (Da):60,702
Checksum:iDB0FAD814A67D91E
GO

Sequence cautioni

The sequence BAC41458.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti372 – 3721N → P in BAE27791 (PubMed:16141072).Curated
Sequence conflicti372 – 3721N → P in BAC41458 (Ref. 3) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei367 – 38923Missing in isoform 3, isoform 4 and isoform 5. 2 PublicationsVSP_022036Add
BLAST
Alternative sequencei409 – 4168Missing in isoform 2 and isoform 4. 1 PublicationVSP_022037
Alternative sequencei581 – 5844VELA → GEWK in isoform 5. 1 PublicationVSP_022038
Alternative sequencei585 – 716132Missing in isoform 5. 1 PublicationVSP_022039Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY313774 mRNA. Translation: AAQ20843.1.
AK147243 mRNA. Translation: BAE27791.1.
AK161513 mRNA. Translation: BAE36436.1.
AB093274 mRNA. Translation: BAC41458.1. Different initiation.
CCDSiCCDS29775.1. [Q7TN99-1]
CCDS70946.1. [Q7TN99-4]
CCDS70947.1. [Q7TN99-3]
RefSeqiNP_001277755.1. NM_001290826.1.
NP_001277756.1. NM_001290827.1.
NP_001277757.1. NM_001290828.1. [Q7TN99-3]
NP_001277758.1. NM_001290829.1.
NP_938042.2. NM_198300.3.
XP_006526892.1. XM_006526829.2.
XP_006526893.1. XM_006526830.2.
XP_006526894.1. XM_006526831.2.
XP_006526895.1. XM_006526832.2.
XP_006526896.1. XM_006526833.2.
XP_006526897.1. XM_006526834.1.
XP_006526902.1. XM_006526839.2.
XP_011245510.1. XM_011247208.1. [Q7TN99-3]
UniGeneiMm.391176.

Genome annotation databases

EnsembliENSMUST00000126188; ENSMUSP00000120416; ENSMUSG00000039652. [Q7TN99-3]
ENSMUST00000126781; ENSMUSP00000122442; ENSMUSG00000039652. [Q7TN99-5]
GeneIDi208922.
KEGGimmu:208922.
UCSCiuc008hhz.2. mouse. [Q7TN99-1]
uc008hia.2. mouse. [Q7TN99-3]
uc008hic.2. mouse. [Q7TN99-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY313774 mRNA. Translation: AAQ20843.1.
AK147243 mRNA. Translation: BAE27791.1.
AK161513 mRNA. Translation: BAE36436.1.
AB093274 mRNA. Translation: BAC41458.1. Different initiation.
CCDSiCCDS29775.1. [Q7TN99-1]
CCDS70946.1. [Q7TN99-4]
CCDS70947.1. [Q7TN99-3]
RefSeqiNP_001277755.1. NM_001290826.1.
NP_001277756.1. NM_001290827.1.
NP_001277757.1. NM_001290828.1. [Q7TN99-3]
NP_001277758.1. NM_001290829.1.
NP_938042.2. NM_198300.3.
XP_006526892.1. XM_006526829.2.
XP_006526893.1. XM_006526830.2.
XP_006526894.1. XM_006526831.2.
XP_006526895.1. XM_006526832.2.
XP_006526896.1. XM_006526833.2.
XP_006526897.1. XM_006526834.1.
XP_006526902.1. XM_006526839.2.
XP_011245510.1. XM_011247208.1. [Q7TN99-3]
UniGeneiMm.391176.

3D structure databases

ProteinModelPortaliQ7TN99.
SMRiQ7TN99. Positions 449-707.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229024. 1 interaction.
IntActiQ7TN99. 2 interactions.
STRINGi10090.ENSMUSP00000078690.

PTM databases

iPTMnetiQ7TN99.
PhosphoSiteiQ7TN99.

Proteomic databases

MaxQBiQ7TN99.
PaxDbiQ7TN99.
PRIDEiQ7TN99.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000126188; ENSMUSP00000120416; ENSMUSG00000039652. [Q7TN99-3]
ENSMUST00000126781; ENSMUSP00000122442; ENSMUSG00000039652. [Q7TN99-5]
GeneIDi208922.
KEGGimmu:208922.
UCSCiuc008hhz.2. mouse. [Q7TN99-1]
uc008hia.2. mouse. [Q7TN99-3]
uc008hic.2. mouse. [Q7TN99-5]

Organism-specific databases

CTDi22849.
MGIiMGI:2443075. Cpeb3.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0129. Eukaryota.
ENOG410Y1XZ. LUCA.
GeneTreeiENSGT00390000012886.
HOGENOMiHOG000290660.
HOVERGENiHBG058010.
InParanoidiQ7TN99.
KOiK02602.
OrthoDBiEOG7X3QR2.
PhylomeDBiQ7TN99.
TreeFamiTF317658.

Miscellaneous databases

ChiTaRSiCpeb3. mouse.
NextBioi372461.
PROiQ7TN99.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TN99.
ExpressionAtlasiQ7TN99. baseline and differential.
GenevisibleiQ7TN99. MM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR032296. CEBP_ZZ.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF16366. CEBP_ZZ. 1 hit.
PF16367. RRM_7. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two previously undescribed members of the mouse CPEB family of genes and their inducible expression in the principal cell layers of the hippocampus."
    Theis M., Si K., Kandel E.R.
    Proc. Natl. Acad. Sci. U.S.A. 100:9602-9607(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), INDUCTION, TISSUE SPECIFICITY.
    Strain: BALB/cJ.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 5).
    Strain: C57BL/6J.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Fetal brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-194 AND SER-197, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart and Kidney.

Entry informationi

Entry nameiCPEB3_MOUSE
AccessioniPrimary (citable) accession number: Q7TN99
Secondary accession number(s): Q3TT89, Q3UHR6, Q8CHC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: October 1, 2003
Last modified: May 11, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.