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Protein

A-kinase anchor protein 7 isoform gamma

Gene

Akap7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably targets cAMP-dependent protein kinase (PKA) to the cellular membrane or cytoskeletal structures. The membrane-associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium (By similarity).By similarityCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95AMPBy similarity1
Binding sitei95CMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi185 – 187AMPBy similarity3
Nucleotide bindingi185 – 187CMPBy similarity3

GO - Molecular functioni

  • nucleotide binding Source: UniProtKB-KW
  • protein kinase A binding Source: MGI
  • protein kinase A regulatory subunit binding Source: UniProtKB
  • protein kinase binding Source: MGI

GO - Biological processi

Keywordsi

LigandNucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 7 isoform gammaBy similarityImported
Short name:
AKAP-7 isoform gammaBy similarity
Alternative name(s):
A-kinase anchor protein 18By similarity
Short name:
AKAP-18By similarity
Protein kinase A-anchoring protein 7 isoform gammaBy similarity
Short name:
PRKA7 isoform gammaBy similarity
Gene namesi
Name:Akap7Imported
Synonyms:Akap18By similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1859150 Akap7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi41K → E: Abolishes nuclear localization; when associated with E-43. 1 Publication1
Mutagenesisi43K → E: Abolishes nuclear localization; when associated with E-41. 1 Publication1
Mutagenesisi265L → P: Abolishes PKA-RI-alpha binding; when associated with P-269. 1 Publication1
Mutagenesisi269L → P: Abolishes PKA-RI-alpha binding; when associated with P-265. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004196341 – 314A-kinase anchor protein 7 isoform gammaAdd BLAST314

Proteomic databases

MaxQBiQ7TN79
PaxDbiQ7TN79
PeptideAtlasiQ7TN79
PRIDEiQ7TN79

PTM databases

iPTMnetiQ7TN79

Expressioni

Tissue specificityi

Expressed in oocytes.1 Publication

Gene expression databases

BgeeiENSMUSG00000039166
ExpressionAtlasiQ7TN79 baseline and differential
GenevisibleiQ7TN79 MM

Interactioni

Subunit structurei

Binds cAMP-dependent protein kinase (PKA). Interacts with PRKCA; only the cytoplasmic form is capable of interacting with PRKCA (By similarity).By similarity1 Publication

GO - Molecular functioni

  • protein kinase A binding Source: MGI
  • protein kinase A regulatory subunit binding Source: UniProtKB
  • protein kinase binding Source: MGI

Protein-protein interaction databases

BioGridi240608, 1 interactor
STRINGi10090.ENSMUSP00000043624

Structurei

3D structure databases

ProteinModelPortaliQ7TN79
SMRiQ7TN79
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni260 – 314PKA-RII-alpha subunit binding domainBy similarityAdd BLAST55
Regioni261 – 285RI-alpha-binding1 PublicationAdd BLAST25
Regioni262 – 275RII-bindingBy similarityAdd BLAST14

Phylogenomic databases

eggNOGiKOG2814 Eukaryota
ENOG4111FMP LUCA
GeneTreeiENSGT00390000012756
HOGENOMiHOG000294077
HOVERGENiHBG050475
InParanoidiQ7TN79
OMAiKLSKAPW
OrthoDBiEOG091G0771
TreeFamiTF105406

Family and domain databases

InterProiView protein in InterPro
IPR019511 AKAP7_RI-RII-bd_dom
IPR009097 cNuc_Pdiesterase
IPR019510 Kinase-A_anchor_nucl_local_sig
PfamiView protein in Pfam
PF10469 AKAP7_NLS, 1 hit
PF10470 AKAP7_RIRII_bdg, 1 hit
SUPFAMiSSF55144 SSF55144, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Gamma1 Publication (identifier: Q7TN79-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPFAAVDIQD DCGSPDVPQA NPKRSKEEEE DRGDKNDHVK KRKKAKKDYQ
60 70 80 90 100
PNYFLSIPIT NKKITTGIKV LQNSILQQDK RLTKAMVGDG SFHITLLVMQ
110 120 130 140 150
LLNEDEVNIG TDALLELKPF VEEILEGKHL ALPFQGIGTF QGQVGFVKLA
160 170 180 190 200
DGDHVSALLE IAETAKRTFR EKGILAGESR TFKPHLTFMK LSKAPMLRKK
210 220 230 240 250
GVRKIEPGLY EQFIDHRFGE ELLYQIDLCS MLKKKQSNGY YHCESSIVIG
260 270 280 290 300
EKDRREPEDA ELVRLSKRLV ENAVLKAVQQ YLEETQNKKQ PGEGNSTKAE
310
EGDRNGDGSD NNRK
Length:314
Mass (Da):35,482
Last modified:October 3, 2012 - v2
Checksum:i67D7D6733D69936F
GO
Isoform AlphaCurated (identifier: O55074-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry O55074.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:81
Mass (Da):9,169
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70V → S in AAP55205 (PubMed:12804576).Curated1
Sequence conflicti278V → A in AAP55205 (PubMed:12804576).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY301068 mRNA Translation: AAP55205.1
AC153549 Genomic DNA No translation available.
AC153550 Genomic DNA No translation available.
CCDSiCCDS23753.1 [Q7TN79-1]
RefSeqiNP_061217.3, NM_018747.4 [Q7TN79-1]
UniGeneiMm.485937
Mm.487103

Genome annotation databases

EnsembliENSMUST00000041984; ENSMUSP00000043624; ENSMUSG00000039166 [Q7TN79-1]
GeneIDi432442
UCSCiuc007eri.1 mouse [Q7TN79-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAKA7G_MOUSE
AccessioniPrimary (citable) accession number: Q7TN79
Secondary accession number(s): F8VQ58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: October 3, 2012
Last modified: April 25, 2018
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health