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Protein

A-kinase anchor protein 7 isoform gamma

Gene

Akap7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably targets cAMP-dependent protein kinase (PKA) to the cellular membrane or cytoskeletal structures. The membrane-associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium (By similarity).By similarityCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95AMPBy similarity1
Binding sitei95CMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi185 – 187AMPBy similarity3
Nucleotide bindingi185 – 187CMPBy similarity3

GO - Molecular functioni

  • nucleotide binding Source: UniProtKB-KW
  • protein kinase A binding Source: MGI
  • protein kinase A regulatory subunit binding Source: UniProtKB
  • protein kinase binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 7 isoform gammaBy similarityImported
Short name:
AKAP-7 isoform gammaBy similarity
Alternative name(s):
A-kinase anchor protein 18By similarity
Short name:
AKAP-18By similarity
Protein kinase A-anchoring protein 7 isoform gammaBy similarity
Short name:
PRKA7 isoform gammaBy similarity
Gene namesi
Name:Akap7Imported
Synonyms:Akap18By similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1859150. Akap7.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • cytoplasm Source: UniProtKB
  • cytosol Source: MGI
  • lateral plasma membrane Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi41K → E: Abolishes nuclear localization; when associated with E-43. 1 Publication1
Mutagenesisi43K → E: Abolishes nuclear localization; when associated with E-41. 1 Publication1
Mutagenesisi265L → P: Abolishes PKA-RI-alpha binding; when associated with P-269. 1 Publication1
Mutagenesisi269L → P: Abolishes PKA-RI-alpha binding; when associated with P-265. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004196341 – 314A-kinase anchor protein 7 isoform gammaAdd BLAST314

Proteomic databases

MaxQBiQ7TN79.
PaxDbiQ7TN79.
PeptideAtlasiQ7TN79.
PRIDEiQ7TN79.

PTM databases

iPTMnetiQ7TN79.

Expressioni

Tissue specificityi

Expressed in oocytes.1 Publication

Gene expression databases

BgeeiENSMUSG00000039166.
ExpressionAtlasiQ7TN79. baseline and differential.
GenevisibleiQ7TN79. MM.

Interactioni

Subunit structurei

Binds cAMP-dependent protein kinase (PKA). Interacts with PRKCA; only the cytoplasmic form is capable of interacting with PRKCA (By similarity).By similarity1 Publication

GO - Molecular functioni

  • protein kinase A binding Source: MGI
  • protein kinase A regulatory subunit binding Source: UniProtKB
  • protein kinase binding Source: MGI

Protein-protein interaction databases

BioGridi240608. 1 interactor.
STRINGi10090.ENSMUSP00000043624.

Structurei

3D structure databases

ProteinModelPortaliQ7TN79.
SMRiQ7TN79.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni260 – 314PKA-RII-alpha subunit binding domainBy similarityAdd BLAST55
Regioni261 – 285RI-alpha-binding1 PublicationAdd BLAST25
Regioni262 – 275RII-bindingBy similarityAdd BLAST14

Phylogenomic databases

eggNOGiKOG2814. Eukaryota.
ENOG4111FMP. LUCA.
GeneTreeiENSGT00390000012756.
HOGENOMiHOG000294077.
HOVERGENiHBG050475.
InParanoidiQ7TN79.
KOiK16524.
OMAiIGEKNGG.
OrthoDBiEOG091G0771.
TreeFamiTF105406.

Family and domain databases

Gene3Di3.90.1140.10. 1 hit.
InterProiIPR019511. AKAP7_RI-RII-bd_dom.
IPR019510. Kinase-A_anchor_nucl_local_sig.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PfamiPF10469. AKAP7_NLS. 1 hit.
PF10470. AKAP7_RIRII_bdg. 1 hit.
[Graphical view]
SUPFAMiSSF55144. SSF55144. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Gamma1 Publication (identifier: Q7TN79-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPFAAVDIQD DCGSPDVPQA NPKRSKEEEE DRGDKNDHVK KRKKAKKDYQ
60 70 80 90 100
PNYFLSIPIT NKKITTGIKV LQNSILQQDK RLTKAMVGDG SFHITLLVMQ
110 120 130 140 150
LLNEDEVNIG TDALLELKPF VEEILEGKHL ALPFQGIGTF QGQVGFVKLA
160 170 180 190 200
DGDHVSALLE IAETAKRTFR EKGILAGESR TFKPHLTFMK LSKAPMLRKK
210 220 230 240 250
GVRKIEPGLY EQFIDHRFGE ELLYQIDLCS MLKKKQSNGY YHCESSIVIG
260 270 280 290 300
EKDRREPEDA ELVRLSKRLV ENAVLKAVQQ YLEETQNKKQ PGEGNSTKAE
310
EGDRNGDGSD NNRK
Length:314
Mass (Da):35,482
Last modified:October 3, 2012 - v2
Checksum:i67D7D6733D69936F
GO
Isoform AlphaCurated (identifier: O55074-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry O55074.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:81
Mass (Da):9,169
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70V → S in AAP55205 (PubMed:12804576).Curated1
Sequence conflicti278V → A in AAP55205 (PubMed:12804576).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY301068 mRNA. Translation: AAP55205.1.
AC153549 Genomic DNA. No translation available.
AC153550 Genomic DNA. No translation available.
CCDSiCCDS23753.1. [Q7TN79-1]
RefSeqiNP_061217.3. NM_018747.4. [Q7TN79-1]
UniGeneiMm.485937.
Mm.487103.

Genome annotation databases

EnsembliENSMUST00000041984; ENSMUSP00000043624; ENSMUSG00000039166. [Q7TN79-1]
GeneIDi432442.
KEGGimmu:432442.
UCSCiuc007eri.1. mouse. [Q7TN79-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY301068 mRNA. Translation: AAP55205.1.
AC153549 Genomic DNA. No translation available.
AC153550 Genomic DNA. No translation available.
CCDSiCCDS23753.1. [Q7TN79-1]
RefSeqiNP_061217.3. NM_018747.4. [Q7TN79-1]
UniGeneiMm.485937.
Mm.487103.

3D structure databases

ProteinModelPortaliQ7TN79.
SMRiQ7TN79.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi240608. 1 interactor.
STRINGi10090.ENSMUSP00000043624.

PTM databases

iPTMnetiQ7TN79.

Proteomic databases

MaxQBiQ7TN79.
PaxDbiQ7TN79.
PeptideAtlasiQ7TN79.
PRIDEiQ7TN79.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041984; ENSMUSP00000043624; ENSMUSG00000039166. [Q7TN79-1]
GeneIDi432442.
KEGGimmu:432442.
UCSCiuc007eri.1. mouse. [Q7TN79-1]

Organism-specific databases

CTDi9465.
MGIiMGI:1859150. Akap7.

Phylogenomic databases

eggNOGiKOG2814. Eukaryota.
ENOG4111FMP. LUCA.
GeneTreeiENSGT00390000012756.
HOGENOMiHOG000294077.
HOVERGENiHBG050475.
InParanoidiQ7TN79.
KOiK16524.
OMAiIGEKNGG.
OrthoDBiEOG091G0771.
TreeFamiTF105406.

Miscellaneous databases

ChiTaRSiAkap7. mouse.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039166.
ExpressionAtlasiQ7TN79. baseline and differential.
GenevisibleiQ7TN79. MM.

Family and domain databases

Gene3Di3.90.1140.10. 1 hit.
InterProiIPR019511. AKAP7_RI-RII-bd_dom.
IPR019510. Kinase-A_anchor_nucl_local_sig.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PfamiPF10469. AKAP7_NLS. 1 hit.
PF10470. AKAP7_RIRII_bdg. 1 hit.
[Graphical view]
SUPFAMiSSF55144. SSF55144. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAKA7G_MOUSE
AccessioniPrimary (citable) accession number: Q7TN79
Secondary accession number(s): F8VQ58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: October 3, 2012
Last modified: November 30, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.