Reviewed, UniProtKB/Swiss-Prot Q7TN78 (ACSM4_RAT)

Names and origin

Protein names

Recommended name:
    Acyl-coenzyme A synthetase ACSM4, mitochondrial
    EC=6.2.1.2
Alternative name(s):
    Olfactory specific medium-chain acyl CoA synthetase
      Short name=O-MACS

Gene names

Name:	Acsm4
Synonyms:	Omacs

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C4 to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro). Ref.1
Catalytic activity	ATP + an acid + CoA = AMP + diphosphate + an acyl-CoA.
Cofactor	Magnesium or manganese By similarity.
Subcellular location	Mitochondrion matrix. Ref.1
Tissue specificity	Detected in adult olfactory epithelium. Ref.1
Developmental stage	Detected in all cell layers of the dorso-medial part of the embryonic olfactory placode and olfactory epithelium. First detected in olfactory placode on embryonic day 11.5. Detected in olfactory placode on embryonic day 12, 14, 16 and 18. Ref.1
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	acyl-CoA metabolic process Ref.1 Inferred from direct assay. Source: RGD sensory perception of smell Ref.1 Non-traceable author statement. Source: RGD
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW butyrate-CoA ligase activity Inferred from electronic annotation. Source: EC fatty-acyl-CoA synthase activity Ref.1 Inferred from direct assay. Source: RGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW odorant binding Ref.1 Non-traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 22	22	Mitochondrion Potential
	Chain	23 – 580	558	Acyl-coenzyme A synthetase ACSM4, mitochondrial		PRO_0000306105
Regions
	Nucleotide binding	229 – 237	9	ATP By similarity
	Nucleotide binding	368 – 373	6	ATP By similarity
Sites
	Binding site	455	1	ATP By similarity
	Binding site	470	1	ATP By similarity
	Binding site	566	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7TN78-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: D9556CB46AAAB7C0	FASTA	580	65,317
	10 20 30 40 50 60 MKVLLRCQRL RFIWLAKPAG RHFHRDPQLW APLTLDDFEA INRCEKPLPK NFNFAADVLD 70 80 90 100 110 120 QWSLKEKSGE RPANPALWWV NGKGDEVKWS FQELGSLSRK AANVLTKPCG LQRGDRVAVI 130 140 150 160 170 180 LPRIPEWWLI NVACMRTGLV FMPGTIQLTR KDILYRLQAS KAKCIVASEE VAPAVDSIAS 190 200 210 220 230 240 ECPNLKTKLL VSPHRWDGWL SFQELLQSAS EEHNCVQTGS QEPMAIYFTS GTTGSPKMAQ 250 260 270 280 290 300 HSQSSLGIGY ALCGRYWLDL TSSDIMWNMS DTGWIKAAIG SVFSTWLRGA CVFVHRMAQF 310 320 330 340 350 360 NTDTFLDTLT SYPITTLCSA PTVYRMLVQQ DLKRYQFKRL RHCLTGGEPL NPEVLEQWKA 370 380 390 400 410 420 QTGLELYEGY GQTEVGIICA NRKGEEIKPG SMGKGVVPYD VQIIDEHGNI LPSGKEGEIA 430 440 450 460 470 480 LRLGSDRPFC FFSEYVDNPE KTDATIRRNF YITGDRGVMD DDGYLWFVGR ADDVIISSGY 490 500 510 520 530 540 RIGPFEVESA LIEHPAVVES AVVSSPDPIR GEVVKAFIVL AAPFKSSNRE KLTAELQDHV 550 560 570 580 KNSTAPYKYP RKVEFVQELP KTITGKIKRN VLRDQEWGRA

References

[1]

"O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, specifically expressed in the olfactory epithelium in a zone-specific manner." Oka Y., Kobayakawa K., Nishizumi H., Miyamichi K., Hirose S., Tsuboi A., Sakano H. Eur. J. Biochem. 270:1995-2004(2003) [PubMed: 12709059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY. Strain: Wistar. Tissue: Olfactory epithelium.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB096688 mRNA. Translation: BAC77614.1.
IPI	IPI00382149.
RefSeq	NP_859046.1.
UniGene	Rn.162462
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q7TN78.
Genome annotation databases
Ensembl	ENSRNOT00000034610; ENSRNOP00000031354; ENSRNOG00000014726; Rattus norvegicus. [Genome view]
GeneID	353317.
KEGG	rno:353317.
UCSC	NM_181695. rat.
Organism-specific databases
CTD	353317.
RGD	727928. Acsm4.
Phylogenomic databases
HOVERGEN	Q7TN78.
OMA	ANPALWW.
Enzyme and pathway databases
BRENDA	6.2.1.2. 248.
Gene expression databases
Genevestigator	Q7TN78.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	672684.

Entry information

Entry name

ACSM4_RAT

Accession

Primary (citable) accession number: Q7TN78

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)