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Protein

Retrotransposon-derived protein PEG10

Gene

Peg10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have a role in cell growth promotion, apoptotic resistance and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1 (By similarity). Involved at the immediate early stage of adipocyte differentiation. May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri344 – 361CCHC-typePROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Differentiation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Retrotransposon-derived protein PEG10
Alternative name(s):
Embryonal carcinoma differentiation regulated protein
Mammalian retrotransposon-derived protein 2
Myelin expression factor 3
Short name:
MyEF-3
Myelin expression factor 3-like protein 1
Short name:
MEF3-like protein 1
Paternally expressed gene 10 protein
Retrotransposon gag domain-containing protein 3
Retrotransposon-derived gag-like polyprotein
Ty3/Gypsy-like protein
Gene namesi
Name:Peg10
Synonyms:Edr, Mar2, Mart2, Mef3l1, Rgag3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2157785. Peg10.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Detected predominantly in the cytoplasm of breast and prostate carcinomas, in hepatocellular carcinoma (HCC) and B-cell chronic lymphocytic leukemia (B-CLL) cells. Colocalized with ALK1 (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality resulting from placental defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003230271 – 958Retrotransposon-derived protein PEG10Add BLAST958

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei753Omega-N-methylarginineCombined sources1
Modified residuei844Omega-N-methylarginineCombined sources1
Modified residuei857Omega-N-methylarginineCombined sources1
Isoform 2 (identifier: Q7TN75-2)
Modified residuei367PhosphoserineCombined sources1

Post-translational modificationi

Isoform 1 undergoes proteolytic cleavage.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ7TN75.
PRIDEiQ7TN75.

PTM databases

iPTMnetiQ7TN75.
PhosphoSitePlusiQ7TN75.

Expressioni

Tissue specificityi

Expressed in brain and testis. Expressed during the early process of adipocyte differentiation. Expressed weakly in mammary gland but strongly in breast carcinomas of a c-MCY-driven transgenic model.3 Publications

Developmental stagei

Expressed in embryo, placenta and amniotic membrane at 10.5 dpc. Expressed in placenta at 9.5 dpc and appeared to increase as gestation progresses peaking around 12.5 and 15.5 dpc (at protein level). Expressed in embryo at 9.5, 12.5 and 16.5 dpc. Expressed in developing musculo-skeletal system, during skeletal myogenesis and also in precartilage primordia and derivative chondrogenic cells of the developing skeleton. Expressed in mesenchymal tissues of developing lung, kidney, gonad, gut and placenta. Not expressed at stages of chondrocyte hypertrophy and ossification of bones. Expressed in all extraembryonic tissues at 9.5 and 12.5 dpc and low-level expression in the embryonic brain and vertebral cartilage at 12.5 dpc.3 Publications

Inductioni

Up-regulated by MYC and during adipocyte differentiation.2 Publications

Gene expression databases

BgeeiENSMUSG00000092035.
CleanExiMM_PEG10.
ExpressionAtlasiQ7TN75. baseline and differential.
GenevisibleiQ7TN75. MM.

Interactioni

Subunit structurei

Interacts with ALK1, SIAH1 and SIAH2.By similarity

Protein-protein interaction databases

BioGridi228380. 1 interactor.
IntActiQ7TN75. 2 interactors.
STRINGi10090.ENSMUSP00000134963.

Structurei

3D structure databases

ProteinModelPortaliQ7TN75.
SMRiQ7TN75.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 326Necessary for interaction with ALK1By similarityAdd BLAST196

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 34Pro-richAdd BLAST24
Compositional biasi35 – 146Asp-richAdd BLAST112
Compositional biasi545 – 733His-richAdd BLAST189
Compositional biasi926 – 951Pro-richAdd BLAST26

Sequence similaritiesi

Contains 1 CCHC-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri344 – 361CCHC-typePROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410J3P7. Eukaryota.
ENOG410Y6K0. LUCA.
GeneTreeiENSGT00840000129904.
HOVERGENiHBG108242.
InParanoidiQ7TN75.

Family and domain databases

InterProiIPR032567. LDOC1-rel.
IPR021109. Peptidase_aspartic_dom.
IPR005162. Retrotrans_gag_dom.
IPR001878. Znf_CCHC.
[Graphical view]
PANTHERiPTHR15503. PTHR15503. 2 hits.
PfamiPF03732. Retrotrans_gag. 1 hit.
[Graphical view]
SUPFAMiSSF50630. SSF50630. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Note: The ribosomal frameshifting efficiency shows an apparent decrease from 68% at 9.5 dpc to 43% by 21.5 dpc during placenta gestation of isoform 1 compared to isoform 2.2 Publications
Isoform 1 (identifier: Q7TN75-1) [UniParc]FASTAAdd to basket
Also known as: RF1/RF2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAGGSSNC PPPPPPPPPN NNNNNNTPKS PGVPDAEDDD ERRHDELPED
60 70 80 90 100
INNFDEDMNR QFENMNLLDQ VELLAQSYSL LDHLDDFDDD DEDDDFDPEP
110 120 130 140 150
DQDELPEYSD DDDLELQGAA AAPIPNFFSD DDCLEDLPEK FDGNPDMLGP
160 170 180 190 200
FMYQCQLFME KSTRDFSVDR IRVCFVTSML IGRAARWATA KLQRCTYLMH
210 220 230 240 250
NYTAFMMELK HVFEDPQRRE AAKRKIRRLR QGPGPVVDYS NAFQMIAQDL
260 270 280 290 300
DWTEPALMDQ FQEGLNPDIR AELSRQEAPK TLAALITACI HIERRLARDA
310 320 330 340 350
AAKPDPSPRA LVMPPNSQTD PTEPVGGARM RLSKEEKERR RKMNLCLYCG
360 370 380 390 400
NGGHFADTCP AKASKNSPPG KLPGPAVGGP SATGPERIRS PPSEASTQHL
410 420 430 440 450
QVMLQIHMPG RPTLFVRAMI DSGASGNFID QDFVIQNAIP LRIKDWPVMV
460 470 480 490 500
EAIDGHPIAS GPIILETHHL IVDLGDHREI LSFDVTQSPF FPIVLGIRWL
510 520 530 540 550
STHDPHITWS TRSIVFNSDY CRLRCRMFAQ IPSNLLFTVP QPNLHPYLLH
560 570 580 590 600
HVHPHVHPHM HQHLHQHLHQ FLHPDPHQYP HPDPHYHHHQ QADMQHQLQQ
610 620 630 640 650
YLYQYLYYHL YPVMHHHLPP DQHEHLHEYL HQYLHQYLHQ FLHHHLHPDL
660 670 680 690 700
HQYLYQYLHN HMNPDPHHHP HPDPPQDPHH PPHQDPHQHP DPHQDPPHQD
710 720 730 740 750
PHQDAHQDPH MDPHLHQHQH PQPQPHPQQH PNHPQQPPFF YHMAGFRIYH
760 770 780 790 800
PVRYYYIQNV YTPVDEHVYP GHRVVDPNIE MIPGAHSLPS GHLYSMSESE
810 820 830 840 850
MNALRNFVDR NVKDGLMTPT VAPNGAQVLQ VKRGWKLQVT YNCRAPQSGT
860 870 880 890 900
IQNQYLRMSL PNMGDPAHLA SYGEFVQVPG YPYPAYVYYT SPHMMTAWYP
910 920 930 940 950
VGRDVHGRII VVPVVITWSQ NTNRQPPVPQ YPPPQPPPPP PPPPPPPPPP

PASSCSAA
Note: Produced by a -1 ribosomal frameshifting due to a slippery site occurring between the codons for Gly-370 and Lys-371.
Length:958
Mass (Da):109,849
Last modified:March 18, 2008 - v2
Checksum:i65BA9B1B6714FE14
GO
Isoform 2 (identifier: Q7TN75-2) [UniParc]FASTAAdd to basket
Also known as: RF1

The sequence of this isoform differs from the canonical sequence as follows:
     371-376: KLPGPA → NSPAPL
     377-958: Missing.

Note: Produced by conventional translation.Combined sources
Show »
Length:376
Mass (Da):42,328
Checksum:i901A5E8275AF611E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti132D → G (PubMed:9473521).Curated1
Sequence conflicti185 – 231ARWAT…RRLRQ → PLGYCQAAKMYLPDAQLHCL YDGAEAVLVRPSESVSVQTQ DQTSAP (PubMed:9473521).CuratedAdd BLAST47
Sequence conflicti660 – 661NH → TI (PubMed:11158386).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_032009371 – 376KLPGPA → NSPAPL in isoform 2. Curated6
Alternative sequenceiVSP_032010377 – 958Missing in isoform 2. CuratedAdd BLAST582

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB091827 mRNA. Translation: BAC77244.1.
AC084315 Genomic DNA. No translation available.
AF302691 Genomic DNA. Translation: AAG39979.1.
CCDSiCCDS57407.1. [Q7TN75-2]
RefSeqiNP_001035701.1. NM_001040611.1. [Q7TN75-2]
NP_570947.2. NM_130877.2.
UniGeneiMm.320575.

Genome annotation databases

EnsembliENSMUST00000176204; ENSMUSP00000134963; ENSMUSG00000092035. [Q7TN75-2]
GeneIDi170676.
KEGGimmu:170676.
UCSCiuc033iob.1. mouse. [Q7TN75-2]

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB091827 mRNA. Translation: BAC77244.1.
AC084315 Genomic DNA. No translation available.
AF302691 Genomic DNA. Translation: AAG39979.1.
CCDSiCCDS57407.1. [Q7TN75-2]
RefSeqiNP_001035701.1. NM_001040611.1. [Q7TN75-2]
NP_570947.2. NM_130877.2.
UniGeneiMm.320575.

3D structure databases

ProteinModelPortaliQ7TN75.
SMRiQ7TN75.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228380. 1 interactor.
IntActiQ7TN75. 2 interactors.
STRINGi10090.ENSMUSP00000134963.

PTM databases

iPTMnetiQ7TN75.
PhosphoSitePlusiQ7TN75.

Proteomic databases

PaxDbiQ7TN75.
PRIDEiQ7TN75.

Protocols and materials databases

DNASUi170676.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000176204; ENSMUSP00000134963; ENSMUSG00000092035. [Q7TN75-2]
GeneIDi170676.
KEGGimmu:170676.
UCSCiuc033iob.1. mouse. [Q7TN75-2]

Organism-specific databases

CTDi23089.
MGIiMGI:2157785. Peg10.

Phylogenomic databases

eggNOGiENOG410J3P7. Eukaryota.
ENOG410Y6K0. LUCA.
GeneTreeiENSGT00840000129904.
HOVERGENiHBG108242.
InParanoidiQ7TN75.

Miscellaneous databases

PROiQ7TN75.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000092035.
CleanExiMM_PEG10.
ExpressionAtlasiQ7TN75. baseline and differential.
GenevisibleiQ7TN75. MM.

Family and domain databases

InterProiIPR032567. LDOC1-rel.
IPR021109. Peptidase_aspartic_dom.
IPR005162. Retrotrans_gag_dom.
IPR001878. Znf_CCHC.
[Graphical view]
PANTHERiPTHR15503. PTHR15503. 2 hits.
PfamiPF03732. Retrotrans_gag. 1 hit.
[Graphical view]
SUPFAMiSSF50630. SSF50630. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEG10_MOUSE
AccessioniPrimary (citable) accession number: Q7TN75
Secondary accession number(s): Q9EQ11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: November 2, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The PEG10 locus is imprinted, giving rise to paternally expressed proteins.
The PEG10 gene can be a target for epigenetic repression, either with or without a gain of promoter methylation, in some of the mammary carcinomas of a c-MCY-driven transgenic model.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome, Transposable element

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.