Q7TN60 (TMC6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transmembrane channel-like protein 6

Gene names

Name:

Tmc6

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	810 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Widely expressed. Ref.1 Ref.2
Sequence similarities	Belongs to the TMC family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7TN60-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7TN60-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-260: Missing.
     781-810: RPGRTQDATEPPAWHEDGGDQKEPCNPRSP → SKLGLVPSGHVCRSPAAAVRAPHIETDVLKLKA

Note: No experimental confirmation available.

Isoform 3 (identifier: Q7TN60-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-360: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q7TN60-4)

The sequence of this isoform differs from the canonical sequence as follows:
     297-328: GRFTHTVMYYGYYSNSTLSPSCDAPREGGQCS → VRPSAPALAKNWCQACPDSPGGLLPVPRYLYG
     329-810: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 810	810	Transmembrane channel-like protein 6		PRO_0000185385
Regions
	Topological domain	1 – 205	205	Lumenal Potential
	Transmembrane	206 – 226	21	Helical; Potential
	Topological domain	227 – 253	27	Cytoplasmic Potential
	Transmembrane	254 – 274	21	Helical; Potential
	Topological domain	275 – 338	64	Lumenal Potential
	Transmembrane	339 – 359	21	Helical; Potential
	Topological domain	360 – 429	70	Cytoplasmic Potential
	Transmembrane	430 – 450	21	Helical; Potential
	Topological domain	451 – 468	18	Lumenal Potential
	Transmembrane	469 – 489	21	Helical; Potential
	Topological domain	490 – 504	15	Cytoplasmic Potential
	Transmembrane	505 – 525	21	Helical; Potential
	Topological domain	526 – 552	27	Lumenal Potential
	Transmembrane	553 – 573	21	Helical; Potential
	Topological domain	574 – 603	30	Cytoplasmic Potential
	Transmembrane	604 – 624	21	Helical; Potential
	Topological domain	625 – 649	25	Lumenal Potential
	Transmembrane	650 – 670	21	Helical; Potential
	Topological domain	671 – 721	51	Cytoplasmic Potential
	Transmembrane	722 – 742	21	Helical; Potential
	Topological domain	743 – 810	68	Lumenal Potential
Amino acid modifications
	Glycosylation	102	1	N-linked (GlcNAc...) Potential
	Glycosylation	311	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 360	360	Missing in isoform 3.		VSP_016443
	Alternative sequence	1 – 260	260	Missing in isoform 2.		VSP_016444
	Alternative sequence	297 – 328	32	GRFTH…GGQCS → VRPSAPALAKNWCQACPDSP GGLLPVPRYLYG in isoform 4.		VSP_016445
	Alternative sequence	329 – 810	482	Missing in isoform 4.		VSP_016446
	Alternative sequence	781 – 810	30	RPGRT…NPRSP → SKLGLVPSGHVCRSPAAAVR APHIETDVLKLKA in isoform 2.		VSP_016447
Experimental info
	Sequence conflict	2	1	A → T in AAP69875. Ref.1
	Sequence conflict	338	1	M → T in AAP69875. Ref.1
	Sequence conflict	365	1	F → L in BAC36945. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 6A78987C46CD4FD5	FASTA	810	90,546
	10 20 30 40 50 60 MAQSLALALD VPETTGDEGL EPSPYEESEV HDSFHQLIQE QSLRVAEEGL ELLPLGLGRG 70 80 90 100 110 120 DQTLPGLEGA PALSSATLRI LASMPSRTIG RSRGAIISQY YNRTVRLRRR SSRPLLGNVV 130 140 150 160 170 180 PSARPSLRLY DLELDSTILE EDEKRSLLVK ELQGLSAAQR DHMVRNMPLS LGEKRCLREK 190 200 210 220 230 240 SWSPKGKRRH LQGRSGAFSC CSRLRYTCML ALHSLGLALL SGLYAARPWR YALKQIGGQF 250 260 270 280 290 300 GSSVLSYFLF LKTLLAFNAL MLLPLLAFLV GVQAAFPPDP AGPVPTFSGL ELLTGGGRFT 310 320 330 340 350 360 HTVMYYGYYS NSTLSPSCDA PREGGQCSPR LGSLPYNMPL AYLFTMGATF FLTCIILVYS 370 380 390 400 410 420 MSHSFGESYR VGSTKGIHAL TVFCSWDYKV TQKRASRVQQ DSICTQLKEL LAEWHLRKRP 430 440 450 460 470 480 RSVCGQLRQV VVLGLGWLLC LGSTMGCTVA VLTFSEVMIQ RPASGGQGVE ALALPLVVSV 490 500 510 520 530 540 LNLGASYLFR GLATLERHDS PVLEVYMAIC RNLILKMAVL GVLCYHWLGR RVATLQGQCW 550 560 570 580 590 600 EDFVGQELYR FMVVDFIFML LDSLFGELVW RLISEKKLKR GQKPEFDIAR NVLDLIYGQT 610 620 630 640 650 660 LTWLGVLFSP LLPAVQILRL LFLFHIKKAS LMANCQAPRR PWLASHMSTV FLTLLCFPSF 670 680 690 700 710 720 LGAAVFLCYA VWQVRPSSTC GPFRTLNTMY EAGTVWVRRL EHAGSGASWL PWLHHFLVEN 730 740 750 760 770 780 TFFLFLASAL LLAVIYFNIQ VVKGQRKVIC LLKEQIRNEG EDKIFLINKL HSVYEEEGRS 790 800 810 RPGRTQDATE PPAWHEDGGD QKEPCNPRSP
	Isoform 2 [UniParc]. Checksum: 89EF622E8B22B66F	FASTA	553	61,806
	10 20 30 40 50 60 MLLPLLAFLV GVQAAFPPDP AGPVPTFSGL ELLTGGGRFT HTVMYYGYYS NSTLSPSCDA 70 80 90 100 110 120 PREGGQCSPR LGSLPYNMPL AYLFTMGATF FLTCIILVYS MSHSFGESYR VGSTKGIHAL 130 140 150 160 170 180 TVFCSWDYKV TQKRASRVQQ DSICTQLKEL LAEWHLRKRP RSVCGQLRQV VVLGLGWLLC 190 200 210 220 230 240 LGSTMGCTVA VLTFSEVMIQ RPASGGQGVE ALALPLVVSV LNLGASYLFR GLATLERHDS 250 260 270 280 290 300 PVLEVYMAIC RNLILKMAVL GVLCYHWLGR RVATLQGQCW EDFVGQELYR FMVVDFIFML 310 320 330 340 350 360 LDSLFGELVW RLISEKKLKR GQKPEFDIAR NVLDLIYGQT LTWLGVLFSP LLPAVQILRL 370 380 390 400 410 420 LFLFHIKKAS LMANCQAPRR PWLASHMSTV FLTLLCFPSF LGAAVFLCYA VWQVRPSSTC 430 440 450 460 470 480 GPFRTLNTMY EAGTVWVRRL EHAGSGASWL PWLHHFLVEN TFFLFLASAL LLAVIYFNIQ 490 500 510 520 530 540 VVKGQRKVIC LLKEQIRNEG EDKIFLINKL HSVYEEEGRS SKLGLVPSGH VCRSPAAAVR 550 APHIETDVLK LKA
	Isoform 3 [UniParc]. Checksum: AF19129C11A53BD0	FASTA	450	51,010
	10 20 30 40 50 60 MSHSFGESYR VGSTKGIHAL TVFCSWDYKV TQKRASRVQQ DSICTQLKEL LAEWHLRKRP 70 80 90 100 110 120 RSVCGQLRQV VVLGLGWLLC LGSTMGCTVA VLTFSEVMIQ RPASGGQGVE ALALPLVVSV 130 140 150 160 170 180 LNLGASYLFR GLATLERHDS PVLEVYMAIC RNLILKMAVL GVLCYHWLGR RVATLQGQCW 190 200 210 220 230 240 EDFVGQELYR FMVVDFIFML LDSLFGELVW RLISEKKLKR GQKPEFDIAR NVLDLIYGQT 250 260 270 280 290 300 LTWLGVLFSP LLPAVQILRL LFLFHIKKAS LMANCQAPRR PWLASHMSTV FLTLLCFPSF 310 320 330 340 350 360 LGAAVFLCYA VWQVRPSSTC GPFRTLNTMY EAGTVWVRRL EHAGSGASWL PWLHHFLVEN 370 380 390 400 410 420 TFFLFLASAL LLAVIYFNIQ VVKGQRKVIC LLKEQIRNEG EDKIFLINKL HSVYEEEGRS 430 440 450 RPGRTQDATE PPAWHEDGGD QKEPCNPRSP
	Isoform 4 [UniParc]. Checksum: EB148AF6E800968B	FASTA	328	35,819
	10 20 30 40 50 60 MAQSLALALD VPETTGDEGL EPSPYEESEV HDSFHQLIQE QSLRVAEEGL ELLPLGLGRG 70 80 90 100 110 120 DQTLPGLEGA PALSSATLRI LASMPSRTIG RSRGAIISQY YNRTVRLRRR SSRPLLGNVV 130 140 150 160 170 180 PSARPSLRLY DLELDSTILE EDEKRSLLVK ELQGLSAAQR DHMVRNMPLS LGEKRCLREK 190 200 210 220 230 240 SWSPKGKRRH LQGRSGAFSC CSRLRYTCML ALHSLGLALL SGLYAARPWR YALKQIGGQF 250 260 270 280 290 300 GSSVLSYFLF LKTLLAFNAL MLLPLLAFLV GVQAAFPPDP AGPVPTFSGL ELLTGGVRPS 310 320 APALAKNWCQ ACPDSPGGLL PVPRYLYG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the transmembrane channel-like (TMC) gene family: functional clues from hearing loss and epidermodysplasia verruciformis." Kurima K., Yang Y., Sorber K., Griffith A.J. Genomics 82:300-308(2003) [PubMed: 12906855] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"TMC and EVER genes belong to a larger novel family, the TMC gene family encoding transmembrane proteins." Keresztes G., Mutai H., Heller S. BMC Genomics 4:24-24(2003) [PubMed: 12812529] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Strain: C57BL/6.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4). Strain: C57BL/6J. Tissue: Mammary gland.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Strain: 129 and FVB/N. Tissue: Kidney and Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY236497 mRNA. Translation: AAP69875.1. AY263158 mRNA. Translation: AAP78773.1. AK052814 mRNA. Translation: BAC35157.1. AK077671 mRNA. Translation: BAC36945.1. AL645856 Genomic DNA. Translation: CAM15525.1. BC004840 mRNA. Translation: AAH04840.2. BC013502 mRNA. Translation: AAH13502.1. BC058195 mRNA. Translation: AAH58195.1.
IPI	IPI00226579. IPI00403990. IPI00468699. IPI00656275.
RefSeq	NP_663414.3. NM_145439.2. NP_851838.1. NM_181321.3.
UniGene	Mm.286963.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7TN60.
PTM databases
PhosphoSite	Q7TN60.
Proteomic databases
PRIDE	Q7TN60.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000103025; ENSMUSP00000099314; ENSMUSG00000025572.
GeneID	217353.
KEGG	mmu:217353.
UCSC	uc007mnn.2. mouse. uc007mnr.2. mouse.
Organism-specific databases
CTD	11322.
MGI	MGI:1098686. Tmc6.
Phylogenomic databases
eggNOG	roNOG08310.
GeneTree	ENSGT00550000074255.
HOGENOM	HBG446241.
HOVERGEN	HBG061579.
InParanoid	Q7TN60.
OrthoDB	EOG4NP73F.
Gene expression databases
ArrayExpress	Q7TN60.
Bgee	Q7TN60.
CleanEx	MM_TMC6.
Genevestigator	Q7TN60.
GermOnline	ENSMUSG00000025572. Mus musculus.
Family and domain databases
InterPro	IPR012496. TMC. [Graphical view]
Pfam	PF07810. TMC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	375801.
SOURCE	Search...

Entry information

Entry name

TMC6_MOUSE

Accession

Primary (citable) accession number: Q7TN60
Secondary accession number(s): B1ATB4 , Q78JR7, Q7TQ68, Q8BP52, Q8C6Z5, Q99J32

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	September 21, 2011
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program