ID   TMC8_MOUSE              Reviewed;         722 AA.
AC   Q7TN58; Q3TAL0; Q3UWI0; Q7TQ67;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 2.
DT   24-JAN-2024, entry version 128.
DE   RecName: Full=Transmembrane channel-like protein 8;
DE   AltName: Full=Epidermodysplasia verruciformis protein 2 homolog;
GN   Name=Tmc8; Synonyms=Ever2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RX   PubMed=12906855; DOI=10.1016/s0888-7543(03)00154-x;
RA   Kurima K., Yang Y., Sorber K., Griffith A.J.;
RT   "Characterization of the transmembrane channel-like (TMC) gene family:
RT   functional clues from hearing loss and epidermodysplasia verruciformis.";
RL   Genomics 82:300-308(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC   STRAIN=C57BL/6J;
RX   PubMed=12812529; DOI=10.1186/1471-2164-4-24;
RA   Keresztes G., Mutai H., Heller S.;
RT   "TMC and EVER genes belong to a larger novel family, the TMC gene family
RT   encoding transmembrane proteins.";
RL   BMC Genomics 4:24-24(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP   [LARGE SCALE MRNA] OF 457-722.
RC   STRAIN=C57BL/6J; TISSUE=Testis;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA   Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of mouse liver.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6; SER-18; SER-658; SER-663;
RP   SER-673 AND SER-698, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP   ANALYSIS].
RC   TISSUE=Kidney, Lung, and Spleen;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Probable ion channel. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with CIB1. {ECO:0000250|UniProtKB:Q8IU68}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q7TN58-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q7TN58-2; Sequence=VSP_016451;
CC   -!- TISSUE SPECIFICITY: Expressed in thymus, lung, prostate, placenta,
CC       testis and spleen. {ECO:0000269|PubMed:12812529,
CC       ECO:0000269|PubMed:12906855}.
CC   -!- SIMILARITY: Belongs to the TMC family. {ECO:0000305}.
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DR   EMBL; AY236501; AAP69879.1; -; mRNA.
DR   EMBL; AY263160; AAP78775.1; -; mRNA.
DR   EMBL; AK136329; BAE22935.1; -; mRNA.
DR   EMBL; AK171772; BAE42658.1; -; mRNA.
DR   CCDS; CCDS25689.1; -. [Q7TN58-1]
DR   CCDS; CCDS56824.1; -. [Q7TN58-2]
DR   RefSeq; NP_001182019.1; NM_001195090.1.
DR   RefSeq; NP_862904.1; NM_181856.2. [Q7TN58-1]
DR   RefSeq; XP_006533187.1; XM_006533124.3.
DR   AlphaFoldDB; Q7TN58; -.
DR   SMR; Q7TN58; -.
DR   BioGRID; 229899; 1.
DR   STRING; 10090.ENSMUSP00000113628; -.
DR   TCDB; 1.A.17.4.2; the calcium-dependent chloride channel (ca-clc) family.
DR   GlyCosmos; Q7TN58; 3 sites, No reported glycans.
DR   GlyGen; Q7TN58; 3 sites.
DR   iPTMnet; Q7TN58; -.
DR   PhosphoSitePlus; Q7TN58; -.
DR   EPD; Q7TN58; -.
DR   jPOST; Q7TN58; -.
DR   MaxQB; Q7TN58; -.
DR   PaxDb; 10090-ENSMUSP00000101941; -.
DR   ProteomicsDB; 259248; -. [Q7TN58-1]
DR   ProteomicsDB; 259249; -. [Q7TN58-2]
DR   Antibodypedia; 32534; 217 antibodies from 28 providers.
DR   DNASU; 217356; -.
DR   Ensembl; ENSMUST00000050874.14; ENSMUSP00000051878.8; ENSMUSG00000050106.19. [Q7TN58-1]
DR   Ensembl; ENSMUST00000106334.9; ENSMUSP00000101941.3; ENSMUSG00000050106.19. [Q7TN58-2]
DR   Ensembl; ENSMUST00000119455.2; ENSMUSP00000113628.2; ENSMUSG00000050106.19. [Q7TN58-2]
DR   GeneID; 217356; -.
DR   KEGG; mmu:217356; -.
DR   UCSC; uc007mns.2; mouse. [Q7TN58-1]
DR   AGR; MGI:2669037; -.
DR   CTD; 147138; -.
DR   MGI; MGI:2669037; Tmc8.
DR   VEuPathDB; HostDB:ENSMUSG00000050106; -.
DR   eggNOG; ENOG502RKT7; Eukaryota.
DR   GeneTree; ENSGT01050000244894; -.
DR   HOGENOM; CLU_013958_3_1_1; -.
DR   InParanoid; Q7TN58; -.
DR   OMA; QCPGSHQ; -.
DR   OrthoDB; 4121853at2759; -.
DR   TreeFam; TF313462; -.
DR   BioGRID-ORCS; 217356; 6 hits in 79 CRISPR screens.
DR   ChiTaRS; Tmc8; mouse.
DR   PRO; PR:Q7TN58; -.
DR   Proteomes; UP000000589; Chromosome 11.
DR   RNAct; Q7TN58; Protein.
DR   Bgee; ENSMUSG00000050106; Expressed in granulocyte and 63 other cell types or tissues.
DR   ExpressionAtlas; Q7TN58; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR   GO; GO:0031965; C:nuclear membrane; ISO:MGI.
DR   GO; GO:0005886; C:plasma membrane; IEA:InterPro.
DR   GO; GO:0008381; F:mechanosensitive monoatomic ion channel activity; IBA:GO_Central.
DR   GO; GO:0140311; F:protein sequestering activity; ISO:MGI.
DR   GO; GO:0043120; F:tumor necrosis factor binding; ISO:MGI.
DR   GO; GO:0006882; P:intracellular zinc ion homeostasis; ISO:MGI.
DR   GO; GO:0031333; P:negative regulation of protein-containing complex assembly; ISO:MGI.
DR   GO; GO:1902041; P:regulation of extrinsic apoptotic signaling pathway via death domain receptors; ISO:MGI.
DR   InterPro; IPR038900; TMC.
DR   InterPro; IPR012496; TMC_dom.
DR   PANTHER; PTHR23302:SF39; TRANSMEMBRANE CHANNEL-LIKE PROTEIN 8; 1.
DR   PANTHER; PTHR23302; TRANSMEMBRANE CHANNEL-RELATED; 1.
DR   Pfam; PF07810; TMC; 1.
DR   Genevisible; Q7TN58; MM.
PE   1: Evidence at protein level;
KW   Alternative splicing; Endoplasmic reticulum; Glycoprotein; Ion channel;
KW   Ion transport; Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..722
FT                   /note="Transmembrane channel-like protein 8"
FT                   /id="PRO_0000185387"
FT   TOPO_DOM        1..118
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        119..139
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        140..204
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        205..225
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        226..307
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        308..328
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        329..375
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        376..396
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        397..430
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        431..451
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        452..492
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        493..513
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        514..536
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        537..557
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        558..598
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        599..619
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        620..722
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          658..722
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..15
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        692..707
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         6
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         18
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         658
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         663
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         673
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         698
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   CARBOHYD        184
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        375
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        571
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         154
FT                   /note="R -> TG (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_016451"
FT   CONFLICT        179
FT                   /note="T -> A (in Ref. 3; BAE42658)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        277..278
FT                   /note="ME -> VC (in Ref. 1; AAP69879)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   722 AA;  82329 MW;  33799843E88B6FD9 CRC64;
     MFRQWSVQSG PAPRRPESQA ASEELWEQEV ERLCASRTPV RMLPYAMADK RFIRELREPE
     GVKTTFWQRW HRPRRVARQH LREAEQRLAR GFGLWEGALY EIGGLFGTGI QSYFTFLRFL
     LLLNLLTMLL TACFVLLPLV WLRPPELGPA LKLRLQCSSS PLPQSDIPRF HNPLWNILTG
     RAFNNTYLFY GAYRAGPESS SEYSIRLAYL LSPMVCLLLC FCGILQRMAE GLPQQTLLGQ
     RYRTPLSAKV FSSWDFCIRV WEAATIKKHE ISNELKMELE EGRRVELAQQ QTRAQKACRL
     LTYLRTNILI VLLVVGAISA IFWATKYSQD NKEESLFLVL QYLPPGVISL VNFLGPQLFT
     VLIQLENYPP GTEVNLTLIW CVVLKLASLG MFSFSLGQTV LCIGRNKTSC ESYGYNACDY
     QCWENSVGEE LYKLIIFNFL LTVAFAFLVS LPRRLLVERF SGWFWTWLDR EEFLVPKNVL
     DIVAAQTVTW MGLFYCPLLP LLNSVFLFLT FYIKKYTLLR NSRASPRRFR ASSSTFFFHL
     VLLLGLLLAA VPLAYVISST HSSWDCGLFT NYSAPWQVVP ELVALQLPLP SQRALRYLSS
     HAFSFPLLIL LSIVLTVCIS QSRANARAIQ GLRKQLVWQV QEKWHLVDDL SRLLPELSPE
     PGSPHSRASR PRSFCPGFPC PGSPGPRTPR LAPSNRLSSS SLGAPSASVP ASRFHFPSRT
     EL
//