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Protein

Transmembrane channel-like protein 8

Gene

Tmc8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable ion channel.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Protein family/group databases

TCDBi1.A.17.4.2. the calcium-dependent chloride channel (ca-clc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane channel-like protein 8
Alternative name(s):
Epidermodysplasia verruciformis protein 2 homolog
Gene namesi
Name:Tmc8
Synonyms:Ever2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2669037. Tmc8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 118118CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei119 – 13921HelicalSequence AnalysisAdd
BLAST
Topological domaini140 – 20465LumenalSequence AnalysisAdd
BLAST
Transmembranei205 – 22521HelicalSequence AnalysisAdd
BLAST
Topological domaini226 – 30782CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei308 – 32821HelicalSequence AnalysisAdd
BLAST
Topological domaini329 – 37547LumenalSequence AnalysisAdd
BLAST
Transmembranei376 – 39621HelicalSequence AnalysisAdd
BLAST
Topological domaini397 – 43034CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei431 – 45121HelicalSequence AnalysisAdd
BLAST
Topological domaini452 – 49241LumenalSequence AnalysisAdd
BLAST
Transmembranei493 – 51321HelicalSequence AnalysisAdd
BLAST
Topological domaini514 – 53623CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei537 – 55721HelicalSequence AnalysisAdd
BLAST
Topological domaini558 – 59841LumenalSequence AnalysisAdd
BLAST
Transmembranei599 – 61921HelicalSequence AnalysisAdd
BLAST
Topological domaini620 – 722103CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 722722Transmembrane channel-like protein 8PRO_0000185387Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi375 – 3751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi571 – 5711N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ7TN58.
PaxDbiQ7TN58.
PRIDEiQ7TN58.

PTM databases

PhosphoSiteiQ7TN58.

Expressioni

Tissue specificityi

Expressed in thymus, lung, prostate, placenta, testis and spleen.2 Publications

Gene expression databases

BgeeiQ7TN58.
CleanExiMM_TMC8.
ExpressionAtlasiQ7TN58. baseline and differential.
GenevisibleiQ7TN58. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000101941.

Structurei

3D structure databases

ProteinModelPortaliQ7TN58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TMC family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG70493.
GeneTreeiENSGT00760000119171.
HOGENOMiHOG000038033.
HOVERGENiHBG055908.
InParanoidiQ7TN58.
OMAiWCVVLKL.
OrthoDBiEOG73FQM9.
TreeFamiTF313462.

Family and domain databases

InterProiIPR012496. TMC.
[Graphical view]
PfamiPF07810. TMC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TN58-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFRQWSVQSG PAPRRPESQA ASEELWEQEV ERLCASRTPV RMLPYAMADK
60 70 80 90 100
RFIRELREPE GVKTTFWQRW HRPRRVARQH LREAEQRLAR GFGLWEGALY
110 120 130 140 150
EIGGLFGTGI QSYFTFLRFL LLLNLLTMLL TACFVLLPLV WLRPPELGPA
160 170 180 190 200
LKLRLQCSSS PLPQSDIPRF HNPLWNILTG RAFNNTYLFY GAYRAGPESS
210 220 230 240 250
SEYSIRLAYL LSPMVCLLLC FCGILQRMAE GLPQQTLLGQ RYRTPLSAKV
260 270 280 290 300
FSSWDFCIRV WEAATIKKHE ISNELKMELE EGRRVELAQQ QTRAQKACRL
310 320 330 340 350
LTYLRTNILI VLLVVGAISA IFWATKYSQD NKEESLFLVL QYLPPGVISL
360 370 380 390 400
VNFLGPQLFT VLIQLENYPP GTEVNLTLIW CVVLKLASLG MFSFSLGQTV
410 420 430 440 450
LCIGRNKTSC ESYGYNACDY QCWENSVGEE LYKLIIFNFL LTVAFAFLVS
460 470 480 490 500
LPRRLLVERF SGWFWTWLDR EEFLVPKNVL DIVAAQTVTW MGLFYCPLLP
510 520 530 540 550
LLNSVFLFLT FYIKKYTLLR NSRASPRRFR ASSSTFFFHL VLLLGLLLAA
560 570 580 590 600
VPLAYVISST HSSWDCGLFT NYSAPWQVVP ELVALQLPLP SQRALRYLSS
610 620 630 640 650
HAFSFPLLIL LSIVLTVCIS QSRANARAIQ GLRKQLVWQV QEKWHLVDDL
660 670 680 690 700
SRLLPELSPE PGSPHSRASR PRSFCPGFPC PGSPGPRTPR LAPSNRLSSS
710 720
SLGAPSASVP ASRFHFPSRT EL
Length:722
Mass (Da):82,329
Last modified:December 6, 2005 - v2
Checksum:i33799843E88B6FD9
GO
Isoform 2 (identifier: Q7TN58-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     154-154: R → TG

Note: No experimental confirmation available.
Show »
Length:723
Mass (Da):82,331
Checksum:i373EF46922479C5D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti179 – 1791T → A in BAE42658 (PubMed:16141072).Curated
Sequence conflicti277 – 2782ME → VC in AAP69879 (PubMed:12906855).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei154 – 1541R → TG in isoform 2. 1 PublicationVSP_016451

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY236501 mRNA. Translation: AAP69879.1.
AY263160 mRNA. Translation: AAP78775.1.
AK136329 mRNA. Translation: BAE22935.1.
AK171772 mRNA. Translation: BAE42658.1.
CCDSiCCDS25689.1. [Q7TN58-1]
CCDS56824.1. [Q7TN58-2]
RefSeqiNP_001182019.1. NM_001195090.1.
NP_862904.1. NM_181856.2. [Q7TN58-1]
XP_006533187.1. XM_006533124.2. [Q7TN58-1]
UniGeneiMm.116675.

Genome annotation databases

EnsembliENSMUST00000050874; ENSMUSP00000051878; ENSMUSG00000050106. [Q7TN58-1]
GeneIDi217356.
UCSCiuc007mns.2. mouse. [Q7TN58-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY236501 mRNA. Translation: AAP69879.1.
AY263160 mRNA. Translation: AAP78775.1.
AK136329 mRNA. Translation: BAE22935.1.
AK171772 mRNA. Translation: BAE42658.1.
CCDSiCCDS25689.1. [Q7TN58-1]
CCDS56824.1. [Q7TN58-2]
RefSeqiNP_001182019.1. NM_001195090.1.
NP_862904.1. NM_181856.2. [Q7TN58-1]
XP_006533187.1. XM_006533124.2. [Q7TN58-1]
UniGeneiMm.116675.

3D structure databases

ProteinModelPortaliQ7TN58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000101941.

Protein family/group databases

TCDBi1.A.17.4.2. the calcium-dependent chloride channel (ca-clc) family.

PTM databases

PhosphoSiteiQ7TN58.

Proteomic databases

MaxQBiQ7TN58.
PaxDbiQ7TN58.
PRIDEiQ7TN58.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050874; ENSMUSP00000051878; ENSMUSG00000050106. [Q7TN58-1]
GeneIDi217356.
UCSCiuc007mns.2. mouse. [Q7TN58-1]

Organism-specific databases

CTDi147138.
MGIiMGI:2669037. Tmc8.

Phylogenomic databases

eggNOGiNOG70493.
GeneTreeiENSGT00760000119171.
HOGENOMiHOG000038033.
HOVERGENiHBG055908.
InParanoidiQ7TN58.
OMAiWCVVLKL.
OrthoDBiEOG73FQM9.
TreeFamiTF313462.

Miscellaneous databases

ChiTaRSiTmc8. mouse.
NextBioi375807.
PROiQ7TN58.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TN58.
CleanExiMM_TMC8.
ExpressionAtlasiQ7TN58. baseline and differential.
GenevisibleiQ7TN58. MM.

Family and domain databases

InterProiIPR012496. TMC.
[Graphical view]
PfamiPF07810. TMC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the transmembrane channel-like (TMC) gene family: functional clues from hearing loss and epidermodysplasia verruciformis."
    Kurima K., Yang Y., Sorber K., Griffith A.J.
    Genomics 82:300-308(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  2. "TMC and EVER genes belong to a larger novel family, the TMC gene family encoding transmembrane proteins."
    Keresztes G., Mutai H., Heller S.
    BMC Genomics 4:24-24(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: C57BL/6.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 457-722.
    Strain: C57BL/6J.
    Tissue: Testis.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiTMC8_MOUSE
AccessioniPrimary (citable) accession number: Q7TN58
Secondary accession number(s): Q3TAL0, Q3UWI0, Q7TQ67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: July 22, 2015
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.