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Q7TN58 (TMC8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transmembrane channel-like protein 8
Alternative name(s):
Epidermodysplasia verruciformis protein 2 homolog
Gene names
Name:Tmc8
Synonyms:Ever2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length722 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable ion channel By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Expressed in thymus, lung, prostate, placenta, testis and spleen. Ref.1 Ref.2

Sequence similarities

Belongs to the TMC family.

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentEndoplasmic reticulum
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   Molecular functionIon channel
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_procession transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q7TN58-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q7TN58-2)

The sequence of this isoform differs from the canonical sequence as follows:
     154-154: R → TG
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 722722Transmembrane channel-like protein 8
PRO_0000185387

Regions

Topological domain1 – 118118Cytoplasmic Potential
Transmembrane119 – 13921Helical; Potential
Topological domain140 – 20465Lumenal Potential
Transmembrane205 – 22521Helical; Potential
Topological domain226 – 30782Cytoplasmic Potential
Transmembrane308 – 32821Helical; Potential
Topological domain329 – 37547Lumenal Potential
Transmembrane376 – 39621Helical; Potential
Topological domain397 – 43034Cytoplasmic Potential
Transmembrane431 – 45121Helical; Potential
Topological domain452 – 49241Lumenal Potential
Transmembrane493 – 51321Helical; Potential
Topological domain514 – 53623Cytoplasmic Potential
Transmembrane537 – 55721Helical; Potential
Topological domain558 – 59841Lumenal Potential
Transmembrane599 – 61921Helical; Potential
Topological domain620 – 722103Cytoplasmic Potential

Amino acid modifications

Glycosylation1841N-linked (GlcNAc...) Potential
Glycosylation3751N-linked (GlcNAc...) Potential
Glycosylation5711N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1541R → TG in isoform 2.
VSP_016451

Experimental info

Sequence conflict1791T → A in BAE42658. Ref.3
Sequence conflict277 – 2782ME → VC in AAP69879. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 6, 2005. Version 2.
Checksum: 33799843E88B6FD9

FASTA72282,329
        10         20         30         40         50         60 
MFRQWSVQSG PAPRRPESQA ASEELWEQEV ERLCASRTPV RMLPYAMADK RFIRELREPE 

        70         80         90        100        110        120 
GVKTTFWQRW HRPRRVARQH LREAEQRLAR GFGLWEGALY EIGGLFGTGI QSYFTFLRFL 

       130        140        150        160        170        180 
LLLNLLTMLL TACFVLLPLV WLRPPELGPA LKLRLQCSSS PLPQSDIPRF HNPLWNILTG 

       190        200        210        220        230        240 
RAFNNTYLFY GAYRAGPESS SEYSIRLAYL LSPMVCLLLC FCGILQRMAE GLPQQTLLGQ 

       250        260        270        280        290        300 
RYRTPLSAKV FSSWDFCIRV WEAATIKKHE ISNELKMELE EGRRVELAQQ QTRAQKACRL 

       310        320        330        340        350        360 
LTYLRTNILI VLLVVGAISA IFWATKYSQD NKEESLFLVL QYLPPGVISL VNFLGPQLFT 

       370        380        390        400        410        420 
VLIQLENYPP GTEVNLTLIW CVVLKLASLG MFSFSLGQTV LCIGRNKTSC ESYGYNACDY 

       430        440        450        460        470        480 
QCWENSVGEE LYKLIIFNFL LTVAFAFLVS LPRRLLVERF SGWFWTWLDR EEFLVPKNVL 

       490        500        510        520        530        540 
DIVAAQTVTW MGLFYCPLLP LLNSVFLFLT FYIKKYTLLR NSRASPRRFR ASSSTFFFHL 

       550        560        570        580        590        600 
VLLLGLLLAA VPLAYVISST HSSWDCGLFT NYSAPWQVVP ELVALQLPLP SQRALRYLSS 

       610        620        630        640        650        660 
HAFSFPLLIL LSIVLTVCIS QSRANARAIQ GLRKQLVWQV QEKWHLVDDL SRLLPELSPE 

       670        680        690        700        710        720 
PGSPHSRASR PRSFCPGFPC PGSPGPRTPR LAPSNRLSSS SLGAPSASVP ASRFHFPSRT 


EL 

« Hide

Isoform 2 [UniParc].

Checksum: 373EF46922479C5D
Show »

FASTA72382,331

References

« Hide 'large scale' references
[1]"Characterization of the transmembrane channel-like (TMC) gene family: functional clues from hearing loss and epidermodysplasia verruciformis."
Kurima K., Yang Y., Sorber K., Griffith A.J.
Genomics 82:300-308(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]"TMC and EVER genes belong to a larger novel family, the TMC gene family encoding transmembrane proteins."
Keresztes G., Mutai H., Heller S.
BMC Genomics 4:24-24(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Strain: C57BL/6.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 457-722.
Strain: C57BL/6J.
Tissue: Testis.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY236501 mRNA. Translation: AAP69879.1.
AY263160 mRNA. Translation: AAP78775.1.
AK136329 mRNA. Translation: BAE22935.1.
AK171772 mRNA. Translation: BAE42658.1.
CCDSCCDS25689.1. [Q7TN58-1]
CCDS56824.1. [Q7TN58-2]
RefSeqNP_001182019.1. NM_001195090.1.
NP_862904.1. NM_181856.2. [Q7TN58-1]
XP_006533187.1. XM_006533124.1. [Q7TN58-1]
UniGeneMm.116675.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB1.A.17.4.2. the calcium-dependent chloride channel (ca-clc) family.

PTM databases

PhosphoSiteQ7TN58.

Proteomic databases

PaxDbQ7TN58.
PRIDEQ7TN58.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000050874; ENSMUSP00000051878; ENSMUSG00000050106. [Q7TN58-1]
ENSMUST00000106334; ENSMUSP00000101941; ENSMUSG00000050106. [Q7TN58-2]
ENSMUST00000119455; ENSMUSP00000113628; ENSMUSG00000050106. [Q7TN58-2]
GeneID217356.
KEGGmmu:217356.
UCSCuc007mns.2. mouse. [Q7TN58-1]

Organism-specific databases

CTD147138.
MGIMGI:2669037. Tmc8.

Phylogenomic databases

eggNOGNOG70493.
GeneTreeENSGT00670000097979.
HOGENOMHOG000038033.
HOVERGENHBG055908.
OMAWCVVLKL.
OrthoDBEOG73FQM9.
TreeFamTF313462.

Gene expression databases

ArrayExpressQ7TN58.
BgeeQ7TN58.
CleanExMM_TMC8.
GenevestigatorQ7TN58.

Family and domain databases

InterProIPR012496. TMC.
[Graphical view]
PfamPF07810. TMC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTMC8. mouse.
NextBio375807.
PROQ7TN58.
SOURCESearch...

Entry information

Entry nameTMC8_MOUSE
AccessionPrimary (citable) accession number: Q7TN58
Secondary accession number(s): Q3TAL0, Q3UWI0, Q7TQ67
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: July 9, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot