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Protein

Stromal membrane-associated protein 2

Gene

Smap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activating protein that acts on ARF1. Can also activate ARF6 (in vitro). May play a role in clathrin-dependent retrograde transport from early endosomes to the trans-Golgi network.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 5124C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Stromal membrane-associated protein 2
Alternative name(s):
Stromal membrane-associated protein 1-like
Gene namesi
Name:Smap2
Synonyms:Smap1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1917030. Smap2.

Subcellular locationi

  • Cytoplasm 1 Publication

  • Note: Detected in multiple foci throughout the cytoplasm and in juxtanuclear structures.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi56 – 561R → Q: Loss of GTPase activation. 1 Publication
Mutagenesisi187 – 1915LLGLD → AAAAA: Loss of interaction with clathrin heavy chains; when associated with 212-AAA-214. 1 Publication
Mutagenesisi212 – 2143DLL → AAA: Loss of interaction with clathrin heavy chains; when associated with 187-AAAAA-191. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Stromal membrane-associated protein 2PRO_0000235842Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei219 – 2191PhosphoserineCombined sources
Modified residuei224 – 2241PhosphoserineBy similarity
Modified residuei230 – 2301PhosphoserineBy similarity
Modified residuei239 – 2391PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7TN29.
MaxQBiQ7TN29.
PaxDbiQ7TN29.
PRIDEiQ7TN29.

PTM databases

iPTMnetiQ7TN29.
PhosphoSiteiQ7TN29.

Expressioni

Gene expression databases

BgeeiQ7TN29.
GenevisibleiQ7TN29. MM.

Interactioni

Subunit structurei

Interacts with ARF1. Interacts with PICALM and clathrin heavy chains.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Smap1Q91VZ64EBI-11358641,EBI-8317690

Protein-protein interaction databases

IntActiQ7TN29. 1 interaction.
STRINGi10090.ENSMUSP00000035800.

Structurei

3D structure databases

ProteinModelPortaliQ7TN29.
SMRiQ7TN29. Positions 10-131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 139127Arf-GAPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 23169Interaction with clathrin heavy chainsAdd
BLAST
Regioni339 – 42890Interaction with PICALMAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi278 – 410133Met-richAdd
BLAST

Sequence similaritiesi

Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 5124C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0703. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00820000127006.
HOGENOMiHOG000230583.
HOVERGENiHBG055260.
InParanoidiQ7TN29.
KOiK12486.
OMAiAQMTQQM.
OrthoDBiEOG78M02F.
PhylomeDBiQ7TN29.
TreeFamiTF313876.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TN29-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTGKSVKDVD RYQAVLANLL LEEDNKFCAD CQSKGPRWAS WNIGVFICIR
60 70 80 90 100
CAGIHRNLGV HISRVKSVNL DQWTQEQIQC MQEMGNGKAN RLYEAYLPET
110 120 130 140 150
FRRPQIDPAV EGFIRDKYEK KKYMDRSLDI NVLRKEKDDK WKRGNEPAPE
160 170 180 190 200
KKMEPVVFEK VKMPQKKEDA QLPRKSSPKS AAPVMDLLGL DAPVACSIAN
210 220 230 240 250
SKTSNALEKD LDLLASVPSP SSVSRKAVGS MPTAGSAGSV PENLNLFPEP
260 270 280 290 300
GSKSEETGKK QLSKDSILSL YGSQTPQMPA QAMFMAPAQM AYPTAYPSFP
310 320 330 340 350
GVTPPNSIMG GMVPPPVGMV AQPGASGMLT PMAMPAGYMG GMQASMMGVP
360 370 380 390 400
NGMMTTQQAG YMASMAAMPQ TVYGVQPAQQ LQWNLTQMTQ QMAGMNFYGA
410 420
NGMMNYGQSM GGGNGQAANQ TLSPQMWK
Length:428
Mass (Da):46,578
Last modified:October 1, 2003 - v1
Checksum:i5F27D6677C3B1AAF
GO
Isoform 2 (identifier: Q7TN29-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     227-245: AVGSMPTAGSAGSVPENLN → SQGANQKKQARNSSPRTPS
     246-428: Missing.

Show »
Length:245
Mass (Da):27,634
Checksum:i50791A8726CAC5E4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891A → V in AAH52413 (PubMed:15489334).Curated
Sequence conflicti179 – 1791K → E in AAH52413 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei227 – 24519AVGSM…PENLN → SQGANQKKQARNSSPRTPS in isoform 2. 1 PublicationVSP_018505Add
BLAST
Alternative sequencei246 – 428183Missing in isoform 2. 1 PublicationVSP_018506Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK152757 mRNA. Translation: BAE31472.1.
BC052413 mRNA. Translation: AAH52413.1.
CCDSiCCDS18598.1. [Q7TN29-1]
RefSeqiNP_598477.2. NM_133716.3. [Q7TN29-1]
UniGeneiMm.271819.

Genome annotation databases

EnsembliENSMUST00000043200; ENSMUSP00000035800; ENSMUSG00000032870. [Q7TN29-1]
GeneIDi69780.
KEGGimmu:69780.
UCSCiuc008unw.2. mouse. [Q7TN29-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK152757 mRNA. Translation: BAE31472.1.
BC052413 mRNA. Translation: AAH52413.1.
CCDSiCCDS18598.1. [Q7TN29-1]
RefSeqiNP_598477.2. NM_133716.3. [Q7TN29-1]
UniGeneiMm.271819.

3D structure databases

ProteinModelPortaliQ7TN29.
SMRiQ7TN29. Positions 10-131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7TN29. 1 interaction.
STRINGi10090.ENSMUSP00000035800.

PTM databases

iPTMnetiQ7TN29.
PhosphoSiteiQ7TN29.

Proteomic databases

EPDiQ7TN29.
MaxQBiQ7TN29.
PaxDbiQ7TN29.
PRIDEiQ7TN29.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043200; ENSMUSP00000035800; ENSMUSG00000032870. [Q7TN29-1]
GeneIDi69780.
KEGGimmu:69780.
UCSCiuc008unw.2. mouse. [Q7TN29-1]

Organism-specific databases

CTDi64744.
MGIiMGI:1917030. Smap2.

Phylogenomic databases

eggNOGiKOG0703. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00820000127006.
HOGENOMiHOG000230583.
HOVERGENiHBG055260.
InParanoidiQ7TN29.
KOiK12486.
OMAiAQMTQQM.
OrthoDBiEOG78M02F.
PhylomeDBiQ7TN29.
TreeFamiTF313876.

Miscellaneous databases

ChiTaRSiSmap2. mouse.
PROiQ7TN29.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TN29.
GenevisibleiQ7TN29. MM.

Family and domain databases

InterProiIPR001164. ArfGAP.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
[Graphical view]
PROSITEiPS50115. ARFGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Bone marrow.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Fetal brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-239, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  4. "SMAP2, a novel ARF GTPase-activating protein, interacts with clathrin and clathrin assembly protein and functions on the AP-1-positive early endosome/trans-Golgi network."
    Natsume W., Tanabe K., Kon S., Yoshida N., Watanabe T., Torii T., Satake M.
    Mol. Biol. Cell 17:2592-2603(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ARF1; PICALM AND CLATHRIN HEAVY CHAINS, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-56; 187-LEU--ASP-191 AND 212-ASP--LEU-214.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-219 AND SER-239, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiSMAP2_MOUSE
AccessioniPrimary (citable) accession number: Q7TN29
Secondary accession number(s): Q3U798
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: October 1, 2003
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.