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Protein

Dapper homolog 2

Gene

Dact2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in regulation of intracellular signaling pathways during development. Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors, such as TGFBR1. May be involved in control of the morphogenetic behavior of kidney ureteric bud cells by keeping cells epithelial and restraining their mesenchymal character. May play an inhibitory role in the re-epithelialization of skin wounds by attenuating TGF-beta signaling.3 Publications

GO - Molecular functioni

  • beta-catenin binding Source: UniProtKB
  • delta-catenin binding Source: UniProtKB
  • protein kinase A binding Source: UniProtKB
  • protein kinase C binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB

GO - Biological processi

  • epithelial cell morphogenesis Source: UniProtKB
  • hematopoietic progenitor cell differentiation Source: MGI
  • inner medullary collecting duct development Source: UniProtKB
  • negative regulation of cell adhesion Source: UniProtKB
  • negative regulation of nodal signaling pathway Source: UniProtKB
  • skin development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Dapper homolog 2
Short name:
mDpr2
Alternative name(s):
Dapper antagonist of catenin 2
Gene namesi
Name:Dact2
Synonyms:Dpr2, Frd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1920347. Dact2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757Dapper homolog 2PRO_0000326200Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7TN08.
PRIDEiQ7TN08.

PTM databases

iPTMnetiQ7TN08.
PhosphoSiteiQ7TN08.

Expressioni

Tissue specificityi

Expressed in kidney (inner medullary collecting duct). Expressed in epidermal keratinocytes and hair follicles.2 Publications

Developmental stagei

Expressed throughout the epiblast at the onset of gastrulation and in somites, the neural tube and gut at later stages of development. In the developing kidney is expressed in the ureteric bud/collecting duct epithelium.3 Publications

Gene expression databases

BgeeiQ7TN08.
GenevisibleiQ7TN08. MM.

Interactioni

Subunit structurei

Can form homodimers and heterodimers with DACT1 or DACT3. Interacts with CSNK1D, PKA catalytic subunit, PKC-type kinase, CSNK2B, DVL1, DVL2, DVL3, VANGL1, VANGL2, TGFBR1, CTNNB1, CTNND2, CTNND1, LEF1, TCF7, TCF7L1 and HDAC1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Dact3Q0PHV72EBI-6392494,EBI-6392520

GO - Molecular functioni

  • beta-catenin binding Source: UniProtKB
  • delta-catenin binding Source: UniProtKB
  • protein kinase A binding Source: UniProtKB
  • protein kinase C binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ7TN08. 24 interactions.
STRINGi10090.ENSMUSP00000051638.

Structurei

3D structure databases

ProteinModelPortaliQ7TN08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 281281Inhibition of Nodal signalingAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili55 – 10753Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi754 – 7574PDZ-bindingBy similarity

Domaini

The C-terminal PDZ-binding motif may mediate interaction with the PDZ domains of DSH (Dishevelled) family proteins.By similarity

Sequence similaritiesi

Belongs to the dapper family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IK2I. Eukaryota.
ENOG410ZHU0. LUCA.
GeneTreeiENSGT00530000063890.
HOGENOMiHOG000112138.
HOVERGENiHBG094869.
InParanoidiQ7TN08.
OMAiHSEYSAE.
OrthoDBiEOG7XH6PQ.
PhylomeDBiQ7TN08.
TreeFamiTF331300.

Family and domain databases

InterProiIPR024853. Dact2.
IPR024843. Dapper.
[Graphical view]
PANTHERiPTHR15919. PTHR15919. 1 hit.
PTHR15919:SF13. PTHR15919:SF13. 1 hit.
PfamiPF15268. Dapper. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TN08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWAPSGQGPA GWDRRRVGAR LRAALAGLQE LQGLRATQQA RVRGALGLHP
60 70 80 90 100
APGPRGQELR LEAALTALRE QLSRLRRQDA GLKTHLDQLD QQISELQLDV
110 120 130 140 150
SRSSCEALDS DSRPSSGFYE LSDAGSCSLS TSCASVCSDR LSPSLGSWLP
160 170 180 190 200
VFQPSKSRSG IGDWRPRSAD ETTVPAWSPQ LTEDSRLLHG AEGTGRLTGM
210 220 230 240 250
FRPRPVSTGD LERVLPADVG LQRAGTDAAH LLGQGIEIPA HALDPTYQRD
260 270 280 290 300
LVARGGQEVY PYPSPLHAVA LQSPLFALPK EAPCFDICSP PQEPPLVPVD
310 320 330 340 350
ENRTQPEPIR ELGSAEAYIH RLLHLRGQEL PLRDVGQEQG GDTAAFPPKP
360 370 380 390 400
CGQRSESTCQ LEKQACGADR GGLKLGRGAA KDSLKQHGPV SLVGAEPLSS
410 420 430 440 450
PLKEETIPWN PCVHGDNTVG SSPCSQAQQP LNDCGQGPVL SPSRVLGTES
460 470 480 490 500
PPLAPEPFAY TSCTTGETSP VKLRMGFSQN KAVKVRRRVS EKVPRLGKQL
510 520 530 540 550
PPQPERQRVT ERDPSRPHQG GLSRRPTLAR EPPGRSCSES TLYPVPFLVP
560 570 580 590 600
VVVAQRESYP TSPQAFFPME AALLSSAARR KQRRWQSTME ISAKAGSVSQ
610 620 630 640 650
PGPSMGLPRS PAKRGSGPRA QSRPTLARQD ACARCESDPS EHSADCTSLY
660 670 680 690 700
HSTIAETSED EEASDHTANR FGDESSSNDS EGCFRGSRRR LAIGSAEAGQ
710 720 730 740 750
GGWAWPRVPP QQPSRAPGNT RPPLPPVPKL CRIKASKALK KKIRRFQPAA

LKVMTMV
Length:757
Mass (Da):81,668
Last modified:March 18, 2008 - v2
Checksum:iDB1A32713B36DFD5
GO

Sequence cautioni

The sequence BAC31003.1 differs from that shown. Reason: Frameshift at position 278. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti640 – 6401S → P in AAP57185 (PubMed:16881060).Curated
Sequence conflicti647 – 6471T → A in AAP57185 (PubMed:16881060).Curated
Sequence conflicti687 – 6871S → G in AAP57185 (PubMed:16881060).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY297430 mRNA. Translation: AAP57185.1.
AK041604 mRNA. Translation: BAC31003.1. Frameshift.
BC058740 mRNA. Translation: AAH58740.1.
CCDSiCCDS28406.1.
RefSeqiNP_766414.3. NM_172826.3.
UniGeneiMm.79993.

Genome annotation databases

EnsembliENSMUST00000053218; ENSMUSP00000051638; ENSMUSG00000048826.
GeneIDi240025.
KEGGimmu:240025.
UCSCiuc008amv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY297430 mRNA. Translation: AAP57185.1.
AK041604 mRNA. Translation: BAC31003.1. Frameshift.
BC058740 mRNA. Translation: AAH58740.1.
CCDSiCCDS28406.1.
RefSeqiNP_766414.3. NM_172826.3.
UniGeneiMm.79993.

3D structure databases

ProteinModelPortaliQ7TN08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7TN08. 24 interactions.
STRINGi10090.ENSMUSP00000051638.

PTM databases

iPTMnetiQ7TN08.
PhosphoSiteiQ7TN08.

Proteomic databases

PaxDbiQ7TN08.
PRIDEiQ7TN08.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053218; ENSMUSP00000051638; ENSMUSG00000048826.
GeneIDi240025.
KEGGimmu:240025.
UCSCiuc008amv.2. mouse.

Organism-specific databases

CTDi168002.
MGIiMGI:1920347. Dact2.

Phylogenomic databases

eggNOGiENOG410IK2I. Eukaryota.
ENOG410ZHU0. LUCA.
GeneTreeiENSGT00530000063890.
HOGENOMiHOG000112138.
HOVERGENiHBG094869.
InParanoidiQ7TN08.
OMAiHSEYSAE.
OrthoDBiEOG7XH6PQ.
PhylomeDBiQ7TN08.
TreeFamiTF331300.

Miscellaneous databases

PROiQ7TN08.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TN08.
GenevisibleiQ7TN08. MM.

Family and domain databases

InterProiIPR024853. Dact2.
IPR024843. Dapper.
[Graphical view]
PANTHERiPTHR15919. PTHR15919. 1 hit.
PTHR15919:SF13. PTHR15919:SF13. 1 hit.
PfamiPF15268. Dapper. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Three Dact gene family members are expressed during embryonic development and in the adult brains of mice."
    Fisher D.A., Kivimaee S., Hoshino J., Suriben R., Martin P.-M., Baxter N., Cheyette B.N.R.
    Dev. Dyn. 235:2620-2630(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Strain: CD-1.
    Tissue: Brain cortex.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Olfactory epithelium.
  4. "The evolutionally conserved activity of Dapper2 in antagonizing TGF-beta signaling."
    Su Y., Zhang L., Gao X., Meng F., Wen J., Zhou H., Meng A., Chen Y.-G.
    FASEB J. 21:682-690(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TGFBR1, DEVELOPMENTAL STAGE.
  5. "Accelerated re-epithelialization in Dpr2-deficient mice is associated with enhanced response to TGFbeta signaling."
    Meng F., Cheng X., Yang L., Hou N., Yang X., Meng A.
    J. Cell Sci. 121:2904-2912(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. "Dact2 is expressed in the developing ureteric bud/collecting duct system of the kidney and controls morphogenetic behavior of collecting duct cells."
    Lee W.C., Hough M.T., Liu W., Ekiert R., Lindstrom N.O., Hohenstein P., Davies J.A.
    Am. J. Physiol. 299:F740-F751(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  7. "All Dact (Dapper/Frodo) scaffold proteins dimerize and exhibit conserved interactions with Vangl, Dvl, and serine/threonine kinases."
    Kivimae S., Yang X.Y., Cheyette B.N.
    BMC Biochem. 12:33-33(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION, SELF-ASSOCIATION, INTERACTION WITH DACT1; DACT3; CSNK1D; PKA; PKC; CSNK2B; DVL1; DVL2; DVL3; VANGL1; VANGL2; TGFBR1; CTNNB1; CTNND2; CTNND1; LEF1; TCF7; TCF7L1 AND HDAC1.

Entry informationi

Entry nameiDACT2_MOUSE
AccessioniPrimary (citable) accession number: Q7TN08
Secondary accession number(s): Q6PDF4, Q8BHZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: June 8, 2016
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Involved in non-canonical Wnt signaling only when massively overexpressed.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.