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Protein

Mediator of RNA polymerase II transcription subunit 26

Gene

Med26

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 26
Alternative name(s):
Cofactor required for Sp1 transcriptional activation subunit 7
Short name:
CRSP complex subunit 7
Mediator complex subunit 26
Gene namesi
Name:Med26
Synonyms:Crsp7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1917875. Med26.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003049591 – 588Mediator of RNA polymerase II transcription subunit 26Add BLAST588

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei435PhosphoserineCombined sources1
Modified residuei458PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7TN02.
MaxQBiQ7TN02.
PaxDbiQ7TN02.
PeptideAtlasiQ7TN02.
PRIDEiQ7TN02.

PTM databases

iPTMnetiQ7TN02.
PhosphoSitePlusiQ7TN02.

Expressioni

Gene expression databases

BgeeiENSMUSG00000045248.
CleanExiMM_MED26.
GenevisibleiQ7TN02. MM.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity). Interacts with CEBPB (when not methylated)(PubMed:20111005).By similarity1 Publication

Protein-protein interaction databases

BioGridi214175. 1 interactor.
IntActiQ7TN02. 7 interactors.
MINTiMINT-7984909.
STRINGi10090.ENSMUSP00000058697.

Structurei

3D structure databases

ProteinModelPortaliQ7TN02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 87TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi159 – 342Pro-richAdd BLAST184

Sequence similaritiesi

Belongs to the Mediator complex subunit 26 family.Curated
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1105. Eukaryota.
COG1594. LUCA.
GeneTreeiENSGT00390000000259.
HOGENOMiHOG000113539.
HOVERGENiHBG051124.
InParanoidiQ7TN02.
KOiK15169.
OMAiHFMSEYL.
OrthoDBiEOG091G0H00.
PhylomeDBiQ7TN02.
TreeFamiTF328436.

Family and domain databases

Gene3Di1.20.930.10. 1 hit.
InterProiIPR031416. Med26_C.
IPR031417. Med26_Mid.
IPR003617. TFIIS/CRSP70_N_sub.
IPR017923. TFIIS_N.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF15693. Med26_C. 1 hit.
PF15694. Med26_M. 1 hit.
[Graphical view]
SMARTiSM00509. TFS2N. 1 hit.
[Graphical view]
SUPFAMiSSF47676. SSF47676. 1 hit.
PROSITEiPS51319. TFIIS_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TN02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAAPASPQQ MRDRLLQAID SQSNIRNMVA VLEVISSLER YPITKEALEE
60 70 80 90 100
TRLGKLINDV RKKTKNEELA KRAKRLLRSW QKLIEPVHQN EVALRALAGA
110 120 130 140 150
AGSANGGAHN CRPEMGVAGA PKSIHDLKNR NDIQRLPGQR LDRLGSRKRR
160 170 180 190 200
GDQRDLGHPG PPHKVSKGSP DPLVPNASPL PTNGISGSPE SLPSPLDGSG
210 220 230 240 250
HLGPDGSRLE PSDNEKHSTK IPVNAVRPRP SSPGLGKPPV PCLQTKAAQL
260 270 280 290 300
QQLDRADESP GPPYPRGSSR CSFSPRNSRH EGSFSRHRSS YIPKGQVSSP
310 320 330 340 350
SPWPQPPDNT QVPSPLPLAQ PPTPPVRRQE LLPNAESPVH WPEQSEGHPR
360 370 380 390 400
LTGPACRAGF SPDSSKADSD ATSSGGSDSK KKKRYRPRDY TVNLDGQVAE
410 420 430 440 450
AGVKPVRLKE RKLTFDPMTR QIRPLTQKEP VRADSPVPTE QLPRTELEQQ
460 470 480 490 500
EVKASLQSPF EQTNWKELSR NEIIQSYLSR QSSLLSSSGA QTPGAHHFMA
510 520 530 540 550
EYLKQEESSR QGARQPHVLL PLPTPTDLPG LTREVTQDDL DRIQAQQWPG
560 570 580
VNGCEDTQGN WYDWTQCISL DPHGDDGRLN ILPYVCLD
Length:588
Mass (Da):64,680
Last modified:October 1, 2003 - v1
Checksum:i0CAFE5930234B293
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64T → N in BAC38738 (PubMed:16141072).Curated1
Sequence conflicti212 – 214SDN → VII in BAB30898 (PubMed:16141072).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017726 mRNA. Translation: BAB30898.1.
AK083035 mRNA. Translation: BAC38738.1.
BC024555 mRNA. Translation: AAH24555.1.
BC054737 mRNA. Translation: AAH54737.1.
CCDSiCCDS22416.1.
RefSeqiNP_081761.2. NM_027485.4.
UniGeneiMm.235885.
Mm.440960.

Genome annotation databases

EnsembliENSMUST00000058534; ENSMUSP00000058697; ENSMUSG00000045248.
GeneIDi70625.
KEGGimmu:70625.
UCSCiuc009mgg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017726 mRNA. Translation: BAB30898.1.
AK083035 mRNA. Translation: BAC38738.1.
BC024555 mRNA. Translation: AAH24555.1.
BC054737 mRNA. Translation: AAH54737.1.
CCDSiCCDS22416.1.
RefSeqiNP_081761.2. NM_027485.4.
UniGeneiMm.235885.
Mm.440960.

3D structure databases

ProteinModelPortaliQ7TN02.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214175. 1 interactor.
IntActiQ7TN02. 7 interactors.
MINTiMINT-7984909.
STRINGi10090.ENSMUSP00000058697.

PTM databases

iPTMnetiQ7TN02.
PhosphoSitePlusiQ7TN02.

Proteomic databases

EPDiQ7TN02.
MaxQBiQ7TN02.
PaxDbiQ7TN02.
PeptideAtlasiQ7TN02.
PRIDEiQ7TN02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058534; ENSMUSP00000058697; ENSMUSG00000045248.
GeneIDi70625.
KEGGimmu:70625.
UCSCiuc009mgg.2. mouse.

Organism-specific databases

CTDi9441.
MGIiMGI:1917875. Med26.

Phylogenomic databases

eggNOGiKOG1105. Eukaryota.
COG1594. LUCA.
GeneTreeiENSGT00390000000259.
HOGENOMiHOG000113539.
HOVERGENiHBG051124.
InParanoidiQ7TN02.
KOiK15169.
OMAiHFMSEYL.
OrthoDBiEOG091G0H00.
PhylomeDBiQ7TN02.
TreeFamiTF328436.

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

PROiQ7TN02.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045248.
CleanExiMM_MED26.
GenevisibleiQ7TN02. MM.

Family and domain databases

Gene3Di1.20.930.10. 1 hit.
InterProiIPR031416. Med26_C.
IPR031417. Med26_Mid.
IPR003617. TFIIS/CRSP70_N_sub.
IPR017923. TFIIS_N.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF15693. Med26_C. 1 hit.
PF15694. Med26_M. 1 hit.
[Graphical view]
SMARTiSM00509. TFS2N. 1 hit.
[Graphical view]
SUPFAMiSSF47676. SSF47676. 1 hit.
PROSITEiPS51319. TFIIS_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMED26_MOUSE
AccessioniPrimary (citable) accession number: Q7TN02
Secondary accession number(s): Q8BUP9, Q8R1G7, Q9CS67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 1, 2003
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.