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Q7TMY8 (HUWE1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase HUWE1

EC=6.3.2.-
Alternative name(s):
E3Histone
HECT, UBA and WWE domain-containing protein 1
Upstream regulatory element-binding protein 1
Short name=URE-B1
Short name=URE-binding protein 1
Gene names
Name:Huwe1
Synonyms:Kiaa0312, Ureb1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length4377 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene By similarity. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation By similarity. Ref.3 Ref.14

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with isoform p19ARFof CDKN2A which strongly inhibits HUWE1 ubiquitin ligase activity By similarity. Interacts with MYCN, POLB and CDC6 By similarity.

Subcellular location

Cytoplasm. Nucleus. Note: Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells. Ref.12

Tissue specificity

Widely expressed. Ref.12

Developmental stage

Expression increases during neuronal differentiation such that the cortical plate contains the highest level. Ref.14

Domain

The HECT domain mediates inhibition of the transcriptional activity of p53 By similarity.

Post-translational modification

Phosphorylated on tyrosine; phosphorylation is probably required for its ability to inhibit TP53 transactivation By similarity.

Sequence similarities

Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.

Contains 1 UBA domain.

Contains 1 UIM (ubiquitin-interacting motif) repeat.

Contains 1 WWE domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q7TMY8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q7TMY8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     3347-3364: FPSHFTQQRTKETNCESD → RSLKESLTPGFFGHQHLG
     3365-4377: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q7TMY8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     3780-3794: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q7TMY8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     3795-3832: SESSNQSETS...DTQSIVISDG → VSMMPVAPHS...LVCFCITFAK
     3833-4377: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 43774377E3 ubiquitin-protein ligase HUWE1
PRO_0000120341

Regions

Domain1316 – 135540UBA
Repeat1370 – 138920UIM
Domain1603 – 168078WWE
Domain4041 – 4377337HECT
Compositional bias2295 – 2469175Glu-rich
Compositional bias2427 – 249064Asp-rich
Compositional bias3484 – 355269Thr-rich

Sites

Active site43441Glycyl thioester intermediate By similarity

Amino acid modifications

Modified residue10841Phosphoserine By similarity
Modified residue13681Phosphoserine By similarity
Modified residue13701Phosphoserine By similarity
Modified residue13951Phosphoserine Ref.13
Modified residue19071Phosphoserine Ref.13 Ref.15
Modified residue22671N6-acetyllysine Ref.16
Modified residue23621Phosphoserine By similarity
Modified residue23651Phosphoserine By similarity
Modified residue23911Phosphoserine By similarity
Modified residue25951Phosphoserine By similarity
Modified residue26191Phosphoserine By similarity
Modified residue27511Phosphothreonine By similarity
Modified residue28871Phosphoserine By similarity
Modified residue29181Phosphoserine By similarity
Modified residue31161Phosphoserine By similarity
Modified residue31271Phosphoserine By similarity
Modified residue36631Phosphoserine By similarity
Modified residue37531Phosphoserine By similarity
Modified residue37581Phosphoserine By similarity
Modified residue37611Phosphoserine By similarity
Modified residue38101Phosphoserine Ref.13
Modified residue38181Phosphoserine By similarity
Modified residue39221Phosphoserine By similarity
Modified residue39271Phosphothreonine By similarity
Modified residue39301Phosphothreonine By similarity

Natural variations

Alternative sequence3347 – 336418FPSHF…NCESD → RSLKESLTPGFFGHQHLG in isoform 2.
VSP_011147
Alternative sequence3365 – 43771013Missing in isoform 2.
VSP_011148
Alternative sequence3780 – 379415Missing in isoform 3.
VSP_011149
Alternative sequence3795 – 383238SESSN…VISDG → VSMMPVAPHSFLYPPSCTMS SVGVHCPYLVCFCITFAK in isoform 4.
VSP_011150
Alternative sequence3833 – 4377545Missing in isoform 4.
VSP_011151

Experimental info

Sequence conflict2531K → R in AAX24124. Ref.3
Sequence conflict2531K → R in AAV90839. Ref.1
Sequence conflict17281S → I in AAV90839. Ref.1
Sequence conflict17281S → I in BAC41411. Ref.5
Sequence conflict20321E → EG in AAV90839. Ref.1
Sequence conflict20321E → EG in BAC41411. Ref.5
Sequence conflict20321E → EG in AAH70444. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 5.
Checksum: 22700C859CD9AF83

FASTA4,377482,635
        10         20         30         40         50         60 
MKVDRTKLKK TPTEAPADCR ALIDKLKVCN DEQLLLELQQ IKTWNIGKCE LYHWVDLLDR 

        70         80         90        100        110        120 
FDGILADAGQ TVENMSWMLV CDRPEKEQLK MLLLAVLNFT ALLIEYSFSR HLYSSIEHLT 

       130        140        150        160        170        180 
TLLASSDMQV VLAVLNLLYV FSKRSNYITR LGSDKRTPLL TRLQHLAESW GGKENGFGLA 

       190        200        210        220        230        240 
ECCRDLQMLK YPPSATTLHF EFYADPGAEV KIEKRTTSNT LHYIHIEQLD KISESPSEIM 

       250        260        270        280        290        300 
ESLTKMYSIP KDKQMLLFTH IRLAHGFSNH RKRLQAVQAR LHAISILVYS NALQESANSI 

       310        320        330        340        350        360 
LYNGLIEELV DVLQITDKQL MEIKAASLRT LTSIVHLERT PKLSSIIDCT GTASYHGFLP 

       370        380        390        400        410        420 
VLVRNCIQAM IDPSMDPYPH QFATALFSFL YHLASYDAGG EALVSCGMME ALLKVIKFLG 

       430        440        450        460        470        480 
DEQDQITFVT RAVRVVDLIT NLDMAAFQSH SGLSIFIYRL EHEVDLCRKE CPFVIKPKIQ 

       490        500        510        520        530        540 
RPSTTQEGEE METDMDGVQC IPQRAALLKS MLNFLKKAIQ DPAFSDGIRH VMDGSLPTSL 

       550        560        570        580        590        600 
KHIISNAEYY GPSLFLLATE VVTVFVFQEP SLLSSLQDNG LTDVMLHALL IKDVPATREV 

       610        620        630        640        650        660 
LGSLPNVFSA LCLNARGLQS FVQCQPFERL FKVLLSPDYL PAMRRRRSSD PLGDTASNLG 

       670        680        690        700        710        720 
SAVDELMRHQ PTLKTDATTA IIKLLEEICN LGRDPKYICQ KPSIQKADGT ATAPPPRSNH 

       730        740        750        760        770        780 
AAEEASSEDE EEEEVQAMQS FNSAQQNETE PNQQVVGTEE RIPIPLMDYI LNVMKFVESI 

       790        800        810        820        830        840 
LSNNTTDDHC QEFVNQKGLL PLVTILGLPN LPIDFPTSAA CQAVAGVCKS ILTLSHEPKV 

       850        860        870        880        890        900 
LQEGLLQLDL ILSSLEPLHR PIESPGGSVL LRELACAGNV ADATLSAQAT PLLHALTAAH 

       910        920        930        940        950        960 
AYIMMFVHTC RVGQSEIRSI SVNQWGSQLG LSVLSKLSQL YCSLVWESTV LLSLCTPNSL 

       970        980        990       1000       1010       1020 
PSGCEFGQAD MQKLVPKDEK AGTTQGGKRS DGEQDGTAGS MDASAQGLLE GIELDGDTLA 

      1030       1040       1050       1060       1070       1080 
PMETDEPSSS DSKGKSKITP AMAARIKQIK PLLSASSRLG RALAELFGLL VKLCVGSPVR 

      1090       1100       1110       1120       1130       1140 
QRRSHHAAST TTAPTPAARS TASALTKLLT KGLSWQPPPY TPTPRFRLTF FICSVGFTSP 

      1150       1160       1170       1180       1190       1200 
MLFDERKYPY HLMLQKFLCS GGHNALFETF NWALSMGGKV PVSEGLEHSD LPDGTGEFLD 

      1210       1220       1230       1240       1250       1260 
AWLMLVEKMV NPTTVLESPH SLPAKLPGGV QSFPQFSALR FLVVTQKAAF TCIKNLWNRK 

      1270       1280       1290       1300       1310       1320 
PLKVYGGRMA ESMLAILCHI LRGEPVIRER LSKEKEGSRG EEEAGQEEGG SRREPQVNQQ 

      1330       1340       1350       1360       1370       1380 
QLQQLMDMGF TREHAMEALL NTSTMEQATE YLLTHPPPII GGVVRDLSMS EEDQMMRAIA 

      1390       1400       1410       1420       1430       1440 
MSLGQDIPMD QRAESPEEVA CRKEEEERKA REKQEEEEAK CLEKFQDADP LEQDELHTFT 

      1450       1460       1470       1480       1490       1500 
DTMLPGCFHL LDELPDTVYR VCDLIMTAIK RNGADYRDMI LKQVVNQVWE AADVLIKAAL 

      1510       1520       1530       1540       1550       1560 
PLTTSDTKTV SEWISQMATL PQASNLATRI LLLTLLFEEL KLPCAWVVES SGILNVLIKL 

      1570       1580       1590       1600       1610       1620 
LEVVQPCLQA AKEQKEVQTP KWITPVLLLI DFYEKTAISS KRRAQMTKYL QSNSNNWRWF 

      1630       1640       1650       1660       1670       1680 
DDRSGRWCSY SASNNSTIDS AWKSGETSVR FTAGRRRYTV QFTTMVQVNE ETGNRRPVML 

      1690       1700       1710       1720       1730       1740 
TLLRVPRLSK NSKSSNGQEL EKTLEESKET DIKHKENKGN DIPLALESTN TEKEASLDET 

      1750       1760       1770       1780       1790       1800 
KIGEILIQGL TEDMVTVLIR ACVSMLGVPV DPDTLHATLR LCLRLTRDHK YAMMFAELKS 

      1810       1820       1830       1840       1850       1860 
TRMILNLTQS SGFNGFTPLV TLLLRHIIED PCTLRHTMEK VVRSAATSGA GSTTSGVVSG 

      1870       1880       1890       1900       1910       1920 
SLGSREINYI LRVLGPAACR NPDIFTEVAN CCIRIALPAP RGSGTASDDE FENLRIKGPN 

      1930       1940       1950       1960       1970       1980 
AVQLVKTTPL KPSSLPVIPD TIKEVIYDML NALAAYHAPE EADKSDPKPG GTTQEVGQLL 

      1990       2000       2010       2020       2030       2040 
QDMGDDVYQQ YRSLTRQSSD FDTQSGFSLN SQVFAADGAP AETSTTGTSQ GEASTPEETR 

      2050       2060       2070       2080       2090       2100 
EGKKDKEGDR TSEEGKQKSK GSKPLMPTST ILRLLAELVR SYVGIATLIA NYSYTVGQSE 

      2110       2120       2130       2140       2150       2160 
LIKEDCSVLA FVLDHLLPHT QNAEDKDTPA LARLFLASLA AAGSGTDAQV ALVNEVKAAL 

      2170       2180       2190       2200       2210       2220 
GRALAMAEST EKHARLQAVM CIISTIMESC PSTSSFYSSA TAKTQHNGMN NIIRLFLKKG 

      2230       2240       2250       2260       2270       2280 
LVNDLARVPH SLDLSSPNMA NTVNAALKPL ETLSRIVNQP SSLFGSKSAS SKNKSEQDAQ 

      2290       2300       2310       2320       2330       2340 
GASQDSSSHQ QDPGEPGEAE VQEEDHDVTQ TEVADGDIMD GEAETDSVVI AGQPEVLSSQ 

      2350       2360       2370       2380       2390       2400 
EMQVENELED LIDELLERDG GSGNSTIIVS RSGEDESQED VLMDEAPSNL SQASTLQANR 

      2410       2420       2430       2440       2450       2460 
EDSMNILDPE DEEEHTQEED SSGSNEDEDD SQDEEEEEEE DEEDDQEDDE GEEGDEDDDD 

      2470       2480       2490       2500       2510       2520 
DGSEMELDED YPDMNASPLV RFERFDREDD LIIEFDNMFS SATDIPPSPG NIPTTHPLMV 

      2530       2540       2550       2560       2570       2580 
RHADHSSLTL GSGSSTTRLT QGIGRSQRTL RQLTANTGHT IHVHYPGNRQ PNPPLILQRL 

      2590       2600       2610       2620       2630       2640 
LGPSAAADIL QLSSSLPLQS RGRARLLVGN DDVHIIARSD DELLDDFFHD QSTATSQAGT 

      2650       2660       2670       2680       2690       2700 
LSSIPTALTR WTEECKVLDA ESMHDCVSVV KVPIVNHLEF LRDEELEERR EKRRKQLAEE 

      2710       2720       2730       2740       2750       2760 
ETKIIDKGKE DKENRDQSAQ CTVTKTNDST EQNVSDGTPM PDSYPTTPSS TDAPTSESKE 

      2770       2780       2790       2800       2810       2820 
TLGTLQPSQQ QPALPPPPSL GEIPQELQSP AEEVANSTQL LMPIELEELG PTRPSGEAET 

      2830       2840       2850       2860       2870       2880 
TQMELSPAPT ITSLSPERAE DSDALTAVSS QLEGSPMDTS SLASCTLEEA VGDTPAAGSS 

      2890       2900       2910       2920       2930       2940 
EQPTAGSSTP GDAPSVVAEV QGRPDVSRES NQPPEDSSPP ASSESSSTRD SAVAISGADS 

      2950       2960       2970       2980       2990       3000 
RGILEEPLPS TSSEEEDPLA GISLPEGVDP SFLAALPDDI RREVLQNQLG IRPPTRSAPS 

      3010       3020       3030       3040       3050       3060 
SNSSAPAVVG NPGVTEVSPE FLAALPPAIQ EEVLAQQRAE QQRRELAQNA SSDTPMDPVT 

      3070       3080       3090       3100       3110       3120 
FIQTLPSDLR RSVLEDMEDS VLAVMPPDIA AEAQALRREQ EARQRQLMHE RLFGHSSTSA 

      3130       3140       3150       3160       3170       3180 
LSAILRSPAF TSRLSGNRGV QYTRLAVQRG GTFQMGGSSS HNRPSGSNVD TLLRLRGRLL 

      3190       3200       3210       3220       3230       3240 
LDHEALSCLL VLLFVDEPKL NTSRLHRVLR NLCYHAQTRH WVIRSLLSIL QRSSESELCI 

      3250       3260       3270       3280       3290       3300 
ETPKLSTSEE RGKKSSKSCA SSSHENRPLD LLHKMESKSS NQLSWLSVSM DAALGCRTNI 

      3310       3320       3330       3340       3350       3360 
FQIQRSGGRK HTEKHASSGS TVHIHPQAAP VVCRHVLDTL IQLAKVFPSH FTQQRTKETN 

      3370       3380       3390       3400       3410       3420 
CESDRERGSK QACSPCSSQS SSSGICTDFW DLLVKLDNMN VSRKGKNSVK SVPVSSGGEG 

      3430       3440       3450       3460       3470       3480 
ETSPHSLEAS PLGQLMNMLS HPVIRRSSLL TEKLLRLLSL ISIALPENKV SEVQTNSSNS 

      3490       3500       3510       3520       3530       3540 
GSSTAATSNT STTTTTTTTA TAPTPTPPAA TTPVTSAPAL VAATAISTIT VAASTTVTTP 

      3550       3560       3570       3580       3590       3600 
TTATTTVSTS TTKGSKSPAK VGEGGSGIDF KMVSSGLTEN QLQLSVEVLT SHSCSEEGLE 

      3610       3620       3630       3640       3650       3660 
DAANVLLQLS RGDSGTRDTV LKLLLNGARH LGYTLCKQIG TLLAELREYN LEQQRRAQCE 

      3670       3680       3690       3700       3710       3720 
TLSPDGLPEE QPQTTKLKGK MQSRFDMAEN VVIVASQKRP LGGRELQLPS MSMLTSKTST 

      3730       3740       3750       3760       3770       3780 
QKFFLRVLQV IIQLRDDTRR ANKKAKQTGR LGSSGLGSAS SIQAAVRQLE AEADAIIQMV 

      3790       3800       3810       3820       3830       3840 
REGQRARRQQ QAATSESSNQ SETSVRREES PMDVDQPSPS AQDTQSIVIS DGTPQGEKEK 

      3850       3860       3870       3880       3890       3900 
EEKPPELPLL SEQLSLDELW DMLGECLKEL EESHDQHAVL VLQPAVEAFF LVHATERESK 

      3910       3920       3930       3940       3950       3960 
PPVRDTRESQ LAHIKDEPPP LSPAPLTPAT PSSLDPFFSR EPSSMHISSS LPPDTQKFLR 

      3970       3980       3990       4000       4010       4020 
FAETHRTVLN QILRQSTTHL ADGPFAVLVD YIRVLDFDVK RKYFRQELER LDEGLRKEDM 

      4030       4040       4050       4060       4070       4080 
AVHVRRDHVF EDSYRELHRK SPEEMKNRLY IVFEGEEGQD AGGLLREWYM IISREMFNPM 

      4090       4100       4110       4120       4130       4140 
YALFRTSPGD RVTYTINPSS HCNPNHLSYF KFVGRIVAKA VYDNRLLECY FTRSFYKHIL 

      4150       4160       4170       4180       4190       4200 
GKSVRYTDME SEDYHFYQGL VYLLENDVST LGYDLTFSTE VQEFGVCEVR DLKPNGANIL 

      4210       4220       4230       4240       4250       4260 
VTEENKKEYV HLVCQMRMTG AIRKQLAAFL EGFYEIIPKR LISIFTEQEL ELLISGLPTI 

      4270       4280       4290       4300       4310       4320 
DIDDLKSNTE YHKYQSNSIQ IQWFWRALRS FDQADRAKFL QFVTGTSKVP LQGFAALEGM 

      4330       4340       4350       4360       4370 
NGIQKFQIHR DDRSTDRLPS AHTCFNQLDL PAYESFEKLR HMLLLAIQEC SEGFGLA 

« Hide

Isoform 2 [UniParc].

Checksum: BD5116429BF0E509
Show »

FASTA3,364369,701
Isoform 3 [UniParc].

Checksum: 0D640BFE13725964
Show »

FASTA4,362480,898
Isoform 4 [UniParc].

Checksum: C8B75208FD6CB0EC
Show »

FASTA3,832419,496

References

« Hide 'large scale' references
[1]"ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor."
Chen D., Kon N., Li M., Zhang W., Qin J., Gu W.
Cell 121:1071-1083(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: C57BL/6J.
[2]Chen D., Kon N., Li M., Zhang W., Qin J., Gu W.
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 253; 1728; 2009-2010; 2032 AND 3034-3035.
[3]"Characterization of E3Histone, a novel testis ubiquitin protein ligase which ubiquitinates histones."
Liu Z., Oughtred R., Wing S.S.
Mol. Cell. Biol. 25:2819-2831(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
Strain: C57BL/6J.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1445-4377 (ISOFORM 1).
Tissue: Embryonic intestine.
[6]Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
Tissue: Brain.
[7]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 1627-1655; 3127-3133 AND 3245-3253, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1630-4377 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3283-4377 (ISOFORM 3).
Strain: C57BL/6 and FVB/N-3.
Tissue: Brain, Eye and Mammary gland.
[9]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3421-4377 (ISOFORM 4).
Strain: C57BL/6J.
[10]"Mouse homolog to KIAA0312."
Seki N., Hattori A., Hayashi A., Kozuma S., Muramatsu M., Saito T.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4005-4377 (ISOFORMS 1/3).
[11]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[12]"Regulated expression of the ubiquitin protein ligase, E3(Histone)/LASU1/Mule/ARF-BP1/HUWE1, during spermatogenesis."
Liu Z., Miao D., Xia Q., Hermo L., Wing S.S.
Dev. Dyn. 236:2889-2898(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[13]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1395; SER-1907 AND SER-3810, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[14]"The HECT-domain ubiquitin ligase Huwe1 controls neural differentiation and proliferation by destabilizing the N-Myc oncoprotein."
Zhao X., Heng J.I.-T., Guardavaccaro D., Jiang R., Pagano M., Guillemot F., Iavarone A., Lasorella A.
Nat. Cell Biol. 10:643-653(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[15]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1907, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[16]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-2267, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY772010 mRNA. Translation: AAV90839.3.
AY929611 mRNA. Translation: AAX24124.1.
BX571795, AL672180, AL954855 Genomic DNA. Translation: CAM13774.1.
AL954855, AL672180, BX571795 Genomic DNA. Translation: CAM17616.1.
AL672180, AL954855, BX571795 Genomic DNA. Translation: CAM23826.1.
AB093227 mRNA. Translation: BAC41411.2.
BC011391 mRNA. Translation: AAH11391.1.
BC017642 mRNA. Translation: AAH17642.2.
BC054372 mRNA. Translation: AAH54372.1.
BC070444 mRNA. Translation: AAH70444.1.
AK083499 mRNA. Translation: BAC38936.1.
AB025966 mRNA. Translation: BAA84697.1.
RefSeqNP_067498.4. NM_021523.4.
UniGeneMm.27372.
Mm.488413.

3D structure databases

ProteinModelPortalQ7TMY8.
SMRQ7TMY8. Positions 1310-1356, 4003-4369.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid208493. 8 interactions.
IntActQ7TMY8. 3 interactions.
MINTMINT-4139570.
STRING10090.ENSMUSP00000026292.

PTM databases

PhosphoSiteQ7TMY8.

Proteomic databases

PaxDbQ7TMY8.
PRIDEQ7TMY8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000112622; ENSMUSP00000108241; ENSMUSG00000025261. [Q7TMY8-1]
GeneID59026.
KEGGmmu:59026.
UCSCuc009upq.2. mouse. [Q7TMY8-1]
uc009upr.1. mouse. [Q7TMY8-2]

Organism-specific databases

CTD10075.
MGIMGI:1926884. Huwe1.
RougeSearch...

Phylogenomic databases

eggNOGCOG5021.
GeneTreeENSGT00750000117291.
HOVERGENHBG080254.
KOK10592.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ7TMY8.
BgeeQ7TMY8.
CleanExMM_HUWE1.
GenevestigatorQ7TMY8.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR025527. DUF4414.
IPR010309. E3_Ub_ligase_DUF908.
IPR010314. E3_Ub_ligase_DUF913.
IPR000569. HECT.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR004170. WWE-dom.
[Graphical view]
PfamPF14377. DUF4414. 1 hit.
PF06012. DUF908. 1 hit.
PF06025. DUF913. 1 hit.
PF00632. HECT. 1 hit.
PF00627. UBA. 1 hit.
PF02825. WWE. 1 hit.
[Graphical view]
SMARTSM00119. HECTc. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMSSF46934. SSF46934. 1 hit.
SSF48371. SSF48371. 3 hits.
SSF56204. SSF56204. 1 hit.
PROSITEPS50237. HECT. 1 hit.
PS50030. UBA. 1 hit.
PS50918. WWE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSHUWE1. mouse.
NextBio314602.
PROQ7TMY8.
SOURCESearch...

Entry information

Entry nameHUWE1_MOUSE
AccessionPrimary (citable) accession number: Q7TMY8
Secondary accession number(s): A2AFQ1 expand/collapse secondary AC list , Q4G2Z1, Q5BMM7, Q6NS61, Q8BNJ7, Q8CFH2, Q8VD14, Q921M5, Q9R0P2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 99 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot