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Q7TMY8

- HUWE1_MOUSE

UniProt

Q7TMY8 - HUWE1_MOUSE

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Protein

E3 ubiquitin-protein ligase HUWE1

Gene

Huwe1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene (By similarity). Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei4344 – 43441Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. ligase activity Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. base-excision repair Source: UniProtKB
  2. cell differentiation Source: UniProtKB-KW
  3. histone ubiquitination Source: UniProtKB
  4. protein monoubiquitination Source: UniProtKB
  5. protein polyubiquitination Source: UniProtKB
  6. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Differentiation, DNA damage, DNA repair, Ubl conjugation pathway

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase HUWE1 (EC:6.3.2.-)
Alternative name(s):
E3Histone
HECT, UBA and WWE domain-containing protein 1
Upstream regulatory element-binding protein 1
Short name:
URE-B1
Short name:
URE-binding protein 1
Gene namesi
Name:Huwe1
Synonyms:Kiaa0312, Ureb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1926884. Huwe1.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication
Note: Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 43774377E3 ubiquitin-protein ligase HUWE1PRO_0000120341Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1084 – 10841PhosphoserineBy similarity
Modified residuei1368 – 13681PhosphoserineBy similarity
Modified residuei1370 – 13701PhosphoserineBy similarity
Modified residuei1395 – 13951Phosphoserine1 Publication
Modified residuei1907 – 19071Phosphoserine2 Publications
Modified residuei2267 – 22671N6-acetyllysine1 Publication
Modified residuei2362 – 23621PhosphoserineBy similarity
Modified residuei2365 – 23651PhosphoserineBy similarity
Modified residuei2391 – 23911PhosphoserineBy similarity
Modified residuei2595 – 25951PhosphoserineBy similarity
Modified residuei2619 – 26191PhosphoserineBy similarity
Modified residuei2751 – 27511PhosphothreonineBy similarity
Modified residuei2887 – 28871PhosphoserineBy similarity
Modified residuei2918 – 29181PhosphoserineBy similarity
Modified residuei3116 – 31161PhosphoserineBy similarity
Modified residuei3127 – 31271PhosphoserineBy similarity
Modified residuei3663 – 36631PhosphoserineBy similarity
Modified residuei3753 – 37531PhosphoserineBy similarity
Modified residuei3758 – 37581PhosphoserineBy similarity
Modified residuei3761 – 37611PhosphoserineBy similarity
Modified residuei3810 – 38101Phosphoserine1 Publication
Modified residuei3818 – 38181PhosphoserineBy similarity
Modified residuei3922 – 39221PhosphoserineBy similarity
Modified residuei3927 – 39271PhosphothreonineBy similarity
Modified residuei3930 – 39301PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylated on tyrosine; phosphorylation is probably required for its ability to inhibit TP53 transactivation.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ7TMY8.
PaxDbiQ7TMY8.
PRIDEiQ7TMY8.

PTM databases

PhosphoSiteiQ7TMY8.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Developmental stagei

Expression increases during neuronal differentiation such that the cortical plate contains the highest level.1 Publication

Gene expression databases

BgeeiQ7TMY8.
CleanExiMM_HUWE1.
ExpressionAtlasiQ7TMY8. baseline and differential.
GenevestigatoriQ7TMY8.

Interactioni

Subunit structurei

Interacts with isoform p19ARF of CDKN2A which strongly inhibits HUWE1 ubiquitin ligase activity. Interacts with MYCN, POLB and CDC6.By similarity

Protein-protein interaction databases

BioGridi208493. 9 interactions.
IntActiQ7TMY8. 3 interactions.
MINTiMINT-4139570.
STRINGi10090.ENSMUSP00000026292.

Structurei

3D structure databases

ProteinModelPortaliQ7TMY8.
SMRiQ7TMY8. Positions 1310-1356, 4003-4369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1316 – 135540UBAPROSITE-ProRule annotationAdd
BLAST
Repeati1370 – 138920UIMAdd
BLAST
Domaini1603 – 168078WWEPROSITE-ProRule annotationAdd
BLAST
Domaini4041 – 4377337HECTPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2295 – 2469175Glu-richAdd
BLAST
Compositional biasi2427 – 249064Asp-richAdd
BLAST
Compositional biasi3484 – 355269Thr-richAdd
BLAST

Domaini

The HECT domain mediates inhibition of the transcriptional activity of p53.By similarity

Sequence similaritiesi

Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation
Contains 1 WWE domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5021.
GeneTreeiENSGT00760000118966.
HOVERGENiHBG080254.
InParanoidiQ7TMY8.
KOiK10592.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR025527. DUF4414.
IPR010309. E3_Ub_ligase_DUF908.
IPR010314. E3_Ub_ligase_DUF913.
IPR000569. HECT.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR004170. WWE-dom.
[Graphical view]
PfamiPF14377. DUF4414. 1 hit.
PF06012. DUF908. 1 hit.
PF06025. DUF913. 1 hit.
PF00632. HECT. 1 hit.
PF00627. UBA. 1 hit.
PF02825. WWE. 1 hit.
[Graphical view]
SMARTiSM00119. HECTc. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF48371. SSF48371. 3 hits.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50237. HECT. 1 hit.
PS50030. UBA. 1 hit.
PS50918. WWE. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q7TMY8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKVDRTKLKK TPTEAPADCR ALIDKLKVCN DEQLLLELQQ IKTWNIGKCE
60 70 80 90 100
LYHWVDLLDR FDGILADAGQ TVENMSWMLV CDRPEKEQLK MLLLAVLNFT
110 120 130 140 150
ALLIEYSFSR HLYSSIEHLT TLLASSDMQV VLAVLNLLYV FSKRSNYITR
160 170 180 190 200
LGSDKRTPLL TRLQHLAESW GGKENGFGLA ECCRDLQMLK YPPSATTLHF
210 220 230 240 250
EFYADPGAEV KIEKRTTSNT LHYIHIEQLD KISESPSEIM ESLTKMYSIP
260 270 280 290 300
KDKQMLLFTH IRLAHGFSNH RKRLQAVQAR LHAISILVYS NALQESANSI
310 320 330 340 350
LYNGLIEELV DVLQITDKQL MEIKAASLRT LTSIVHLERT PKLSSIIDCT
360 370 380 390 400
GTASYHGFLP VLVRNCIQAM IDPSMDPYPH QFATALFSFL YHLASYDAGG
410 420 430 440 450
EALVSCGMME ALLKVIKFLG DEQDQITFVT RAVRVVDLIT NLDMAAFQSH
460 470 480 490 500
SGLSIFIYRL EHEVDLCRKE CPFVIKPKIQ RPSTTQEGEE METDMDGVQC
510 520 530 540 550
IPQRAALLKS MLNFLKKAIQ DPAFSDGIRH VMDGSLPTSL KHIISNAEYY
560 570 580 590 600
GPSLFLLATE VVTVFVFQEP SLLSSLQDNG LTDVMLHALL IKDVPATREV
610 620 630 640 650
LGSLPNVFSA LCLNARGLQS FVQCQPFERL FKVLLSPDYL PAMRRRRSSD
660 670 680 690 700
PLGDTASNLG SAVDELMRHQ PTLKTDATTA IIKLLEEICN LGRDPKYICQ
710 720 730 740 750
KPSIQKADGT ATAPPPRSNH AAEEASSEDE EEEEVQAMQS FNSAQQNETE
760 770 780 790 800
PNQQVVGTEE RIPIPLMDYI LNVMKFVESI LSNNTTDDHC QEFVNQKGLL
810 820 830 840 850
PLVTILGLPN LPIDFPTSAA CQAVAGVCKS ILTLSHEPKV LQEGLLQLDL
860 870 880 890 900
ILSSLEPLHR PIESPGGSVL LRELACAGNV ADATLSAQAT PLLHALTAAH
910 920 930 940 950
AYIMMFVHTC RVGQSEIRSI SVNQWGSQLG LSVLSKLSQL YCSLVWESTV
960 970 980 990 1000
LLSLCTPNSL PSGCEFGQAD MQKLVPKDEK AGTTQGGKRS DGEQDGTAGS
1010 1020 1030 1040 1050
MDASAQGLLE GIELDGDTLA PMETDEPSSS DSKGKSKITP AMAARIKQIK
1060 1070 1080 1090 1100
PLLSASSRLG RALAELFGLL VKLCVGSPVR QRRSHHAAST TTAPTPAARS
1110 1120 1130 1140 1150
TASALTKLLT KGLSWQPPPY TPTPRFRLTF FICSVGFTSP MLFDERKYPY
1160 1170 1180 1190 1200
HLMLQKFLCS GGHNALFETF NWALSMGGKV PVSEGLEHSD LPDGTGEFLD
1210 1220 1230 1240 1250
AWLMLVEKMV NPTTVLESPH SLPAKLPGGV QSFPQFSALR FLVVTQKAAF
1260 1270 1280 1290 1300
TCIKNLWNRK PLKVYGGRMA ESMLAILCHI LRGEPVIRER LSKEKEGSRG
1310 1320 1330 1340 1350
EEEAGQEEGG SRREPQVNQQ QLQQLMDMGF TREHAMEALL NTSTMEQATE
1360 1370 1380 1390 1400
YLLTHPPPII GGVVRDLSMS EEDQMMRAIA MSLGQDIPMD QRAESPEEVA
1410 1420 1430 1440 1450
CRKEEEERKA REKQEEEEAK CLEKFQDADP LEQDELHTFT DTMLPGCFHL
1460 1470 1480 1490 1500
LDELPDTVYR VCDLIMTAIK RNGADYRDMI LKQVVNQVWE AADVLIKAAL
1510 1520 1530 1540 1550
PLTTSDTKTV SEWISQMATL PQASNLATRI LLLTLLFEEL KLPCAWVVES
1560 1570 1580 1590 1600
SGILNVLIKL LEVVQPCLQA AKEQKEVQTP KWITPVLLLI DFYEKTAISS
1610 1620 1630 1640 1650
KRRAQMTKYL QSNSNNWRWF DDRSGRWCSY SASNNSTIDS AWKSGETSVR
1660 1670 1680 1690 1700
FTAGRRRYTV QFTTMVQVNE ETGNRRPVML TLLRVPRLSK NSKSSNGQEL
1710 1720 1730 1740 1750
EKTLEESKET DIKHKENKGN DIPLALESTN TEKEASLDET KIGEILIQGL
1760 1770 1780 1790 1800
TEDMVTVLIR ACVSMLGVPV DPDTLHATLR LCLRLTRDHK YAMMFAELKS
1810 1820 1830 1840 1850
TRMILNLTQS SGFNGFTPLV TLLLRHIIED PCTLRHTMEK VVRSAATSGA
1860 1870 1880 1890 1900
GSTTSGVVSG SLGSREINYI LRVLGPAACR NPDIFTEVAN CCIRIALPAP
1910 1920 1930 1940 1950
RGSGTASDDE FENLRIKGPN AVQLVKTTPL KPSSLPVIPD TIKEVIYDML
1960 1970 1980 1990 2000
NALAAYHAPE EADKSDPKPG GTTQEVGQLL QDMGDDVYQQ YRSLTRQSSD
2010 2020 2030 2040 2050
FDTQSGFSLN SQVFAADGAP AETSTTGTSQ GEASTPEETR EGKKDKEGDR
2060 2070 2080 2090 2100
TSEEGKQKSK GSKPLMPTST ILRLLAELVR SYVGIATLIA NYSYTVGQSE
2110 2120 2130 2140 2150
LIKEDCSVLA FVLDHLLPHT QNAEDKDTPA LARLFLASLA AAGSGTDAQV
2160 2170 2180 2190 2200
ALVNEVKAAL GRALAMAEST EKHARLQAVM CIISTIMESC PSTSSFYSSA
2210 2220 2230 2240 2250
TAKTQHNGMN NIIRLFLKKG LVNDLARVPH SLDLSSPNMA NTVNAALKPL
2260 2270 2280 2290 2300
ETLSRIVNQP SSLFGSKSAS SKNKSEQDAQ GASQDSSSHQ QDPGEPGEAE
2310 2320 2330 2340 2350
VQEEDHDVTQ TEVADGDIMD GEAETDSVVI AGQPEVLSSQ EMQVENELED
2360 2370 2380 2390 2400
LIDELLERDG GSGNSTIIVS RSGEDESQED VLMDEAPSNL SQASTLQANR
2410 2420 2430 2440 2450
EDSMNILDPE DEEEHTQEED SSGSNEDEDD SQDEEEEEEE DEEDDQEDDE
2460 2470 2480 2490 2500
GEEGDEDDDD DGSEMELDED YPDMNASPLV RFERFDREDD LIIEFDNMFS
2510 2520 2530 2540 2550
SATDIPPSPG NIPTTHPLMV RHADHSSLTL GSGSSTTRLT QGIGRSQRTL
2560 2570 2580 2590 2600
RQLTANTGHT IHVHYPGNRQ PNPPLILQRL LGPSAAADIL QLSSSLPLQS
2610 2620 2630 2640 2650
RGRARLLVGN DDVHIIARSD DELLDDFFHD QSTATSQAGT LSSIPTALTR
2660 2670 2680 2690 2700
WTEECKVLDA ESMHDCVSVV KVPIVNHLEF LRDEELEERR EKRRKQLAEE
2710 2720 2730 2740 2750
ETKIIDKGKE DKENRDQSAQ CTVTKTNDST EQNVSDGTPM PDSYPTTPSS
2760 2770 2780 2790 2800
TDAPTSESKE TLGTLQPSQQ QPALPPPPSL GEIPQELQSP AEEVANSTQL
2810 2820 2830 2840 2850
LMPIELEELG PTRPSGEAET TQMELSPAPT ITSLSPERAE DSDALTAVSS
2860 2870 2880 2890 2900
QLEGSPMDTS SLASCTLEEA VGDTPAAGSS EQPTAGSSTP GDAPSVVAEV
2910 2920 2930 2940 2950
QGRPDVSRES NQPPEDSSPP ASSESSSTRD SAVAISGADS RGILEEPLPS
2960 2970 2980 2990 3000
TSSEEEDPLA GISLPEGVDP SFLAALPDDI RREVLQNQLG IRPPTRSAPS
3010 3020 3030 3040 3050
SNSSAPAVVG NPGVTEVSPE FLAALPPAIQ EEVLAQQRAE QQRRELAQNA
3060 3070 3080 3090 3100
SSDTPMDPVT FIQTLPSDLR RSVLEDMEDS VLAVMPPDIA AEAQALRREQ
3110 3120 3130 3140 3150
EARQRQLMHE RLFGHSSTSA LSAILRSPAF TSRLSGNRGV QYTRLAVQRG
3160 3170 3180 3190 3200
GTFQMGGSSS HNRPSGSNVD TLLRLRGRLL LDHEALSCLL VLLFVDEPKL
3210 3220 3230 3240 3250
NTSRLHRVLR NLCYHAQTRH WVIRSLLSIL QRSSESELCI ETPKLSTSEE
3260 3270 3280 3290 3300
RGKKSSKSCA SSSHENRPLD LLHKMESKSS NQLSWLSVSM DAALGCRTNI
3310 3320 3330 3340 3350
FQIQRSGGRK HTEKHASSGS TVHIHPQAAP VVCRHVLDTL IQLAKVFPSH
3360 3370 3380 3390 3400
FTQQRTKETN CESDRERGSK QACSPCSSQS SSSGICTDFW DLLVKLDNMN
3410 3420 3430 3440 3450
VSRKGKNSVK SVPVSSGGEG ETSPHSLEAS PLGQLMNMLS HPVIRRSSLL
3460 3470 3480 3490 3500
TEKLLRLLSL ISIALPENKV SEVQTNSSNS GSSTAATSNT STTTTTTTTA
3510 3520 3530 3540 3550
TAPTPTPPAA TTPVTSAPAL VAATAISTIT VAASTTVTTP TTATTTVSTS
3560 3570 3580 3590 3600
TTKGSKSPAK VGEGGSGIDF KMVSSGLTEN QLQLSVEVLT SHSCSEEGLE
3610 3620 3630 3640 3650
DAANVLLQLS RGDSGTRDTV LKLLLNGARH LGYTLCKQIG TLLAELREYN
3660 3670 3680 3690 3700
LEQQRRAQCE TLSPDGLPEE QPQTTKLKGK MQSRFDMAEN VVIVASQKRP
3710 3720 3730 3740 3750
LGGRELQLPS MSMLTSKTST QKFFLRVLQV IIQLRDDTRR ANKKAKQTGR
3760 3770 3780 3790 3800
LGSSGLGSAS SIQAAVRQLE AEADAIIQMV REGQRARRQQ QAATSESSNQ
3810 3820 3830 3840 3850
SETSVRREES PMDVDQPSPS AQDTQSIVIS DGTPQGEKEK EEKPPELPLL
3860 3870 3880 3890 3900
SEQLSLDELW DMLGECLKEL EESHDQHAVL VLQPAVEAFF LVHATERESK
3910 3920 3930 3940 3950
PPVRDTRESQ LAHIKDEPPP LSPAPLTPAT PSSLDPFFSR EPSSMHISSS
3960 3970 3980 3990 4000
LPPDTQKFLR FAETHRTVLN QILRQSTTHL ADGPFAVLVD YIRVLDFDVK
4010 4020 4030 4040 4050
RKYFRQELER LDEGLRKEDM AVHVRRDHVF EDSYRELHRK SPEEMKNRLY
4060 4070 4080 4090 4100
IVFEGEEGQD AGGLLREWYM IISREMFNPM YALFRTSPGD RVTYTINPSS
4110 4120 4130 4140 4150
HCNPNHLSYF KFVGRIVAKA VYDNRLLECY FTRSFYKHIL GKSVRYTDME
4160 4170 4180 4190 4200
SEDYHFYQGL VYLLENDVST LGYDLTFSTE VQEFGVCEVR DLKPNGANIL
4210 4220 4230 4240 4250
VTEENKKEYV HLVCQMRMTG AIRKQLAAFL EGFYEIIPKR LISIFTEQEL
4260 4270 4280 4290 4300
ELLISGLPTI DIDDLKSNTE YHKYQSNSIQ IQWFWRALRS FDQADRAKFL
4310 4320 4330 4340 4350
QFVTGTSKVP LQGFAALEGM NGIQKFQIHR DDRSTDRLPS AHTCFNQLDL
4360 4370
PAYESFEKLR HMLLLAIQEC SEGFGLA
Length:4,377
Mass (Da):482,635
Last modified:July 27, 2011 - v5
Checksum:i22700C859CD9AF83
GO
Isoform 2 (identifier: Q7TMY8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     3347-3364: FPSHFTQQRTKETNCESD → RSLKESLTPGFFGHQHLG
     3365-4377: Missing.

Note: No experimental confirmation available.

Show »
Length:3,364
Mass (Da):369,701
Checksum:iBD5116429BF0E509
GO
Isoform 3 (identifier: Q7TMY8-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     3780-3794: Missing.

Note: No experimental confirmation available.

Show »
Length:4,362
Mass (Da):480,898
Checksum:i0D640BFE13725964
GO
Isoform 4 (identifier: Q7TMY8-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     3795-3832: SESSNQSETS...DTQSIVISDG → VSMMPVAPHS...LVCFCITFAK
     3833-4377: Missing.

Note: No experimental confirmation available.

Show »
Length:3,832
Mass (Da):419,496
Checksum:iC8B75208FD6CB0EC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti253 – 2531K → R in AAX24124. (PubMed:15767685)Curated
Sequence conflicti253 – 2531K → R in AAV90839. (PubMed:15989956)Curated
Sequence conflicti1728 – 17281S → I in AAV90839. (PubMed:15989956)Curated
Sequence conflicti1728 – 17281S → I in BAC41411. (PubMed:12465718)Curated
Sequence conflicti2032 – 20321E → EG in AAV90839. (PubMed:15989956)Curated
Sequence conflicti2032 – 20321E → EG in BAC41411. (PubMed:12465718)Curated
Sequence conflicti2032 – 20321E → EG in AAH70444. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei3347 – 336418FPSHF…NCESD → RSLKESLTPGFFGHQHLG in isoform 2. 1 PublicationVSP_011147Add
BLAST
Alternative sequencei3365 – 43771013Missing in isoform 2. 1 PublicationVSP_011148Add
BLAST
Alternative sequencei3780 – 379415Missing in isoform 3. 1 PublicationVSP_011149Add
BLAST
Alternative sequencei3795 – 383238SESSN…VISDG → VSMMPVAPHSFLYPPSCTMS SVGVHCPYLVCFCITFAK in isoform 4. 1 PublicationVSP_011150Add
BLAST
Alternative sequencei3833 – 4377545Missing in isoform 4. 1 PublicationVSP_011151Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY772010 mRNA. Translation: AAV90839.3.
AY929611 mRNA. Translation: AAX24124.1.
BX571795, AL672180, AL954855 Genomic DNA. Translation: CAM13774.1.
AL954855, AL672180, BX571795 Genomic DNA. Translation: CAM17616.1.
AL672180, AL954855, BX571795 Genomic DNA. Translation: CAM23826.1.
AB093227 mRNA. Translation: BAC41411.2.
BC011391 mRNA. Translation: AAH11391.1.
BC017642 mRNA. Translation: AAH17642.2.
BC054372 mRNA. Translation: AAH54372.1.
BC070444 mRNA. Translation: AAH70444.1.
AK083499 mRNA. Translation: BAC38936.1.
AB025966 mRNA. Translation: BAA84697.1.
RefSeqiNP_067498.4. NM_021523.4.
UniGeneiMm.27372.
Mm.488413.

Genome annotation databases

EnsembliENSMUST00000112622; ENSMUSP00000108241; ENSMUSG00000025261. [Q7TMY8-1]
GeneIDi59026.
KEGGimmu:59026.
UCSCiuc009upq.2. mouse. [Q7TMY8-1]
uc009upr.1. mouse. [Q7TMY8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY772010 mRNA. Translation: AAV90839.3 .
AY929611 mRNA. Translation: AAX24124.1 .
BX571795 , AL672180 , AL954855 Genomic DNA. Translation: CAM13774.1 .
AL954855 , AL672180 , BX571795 Genomic DNA. Translation: CAM17616.1 .
AL672180 , AL954855 , BX571795 Genomic DNA. Translation: CAM23826.1 .
AB093227 mRNA. Translation: BAC41411.2 .
BC011391 mRNA. Translation: AAH11391.1 .
BC017642 mRNA. Translation: AAH17642.2 .
BC054372 mRNA. Translation: AAH54372.1 .
BC070444 mRNA. Translation: AAH70444.1 .
AK083499 mRNA. Translation: BAC38936.1 .
AB025966 mRNA. Translation: BAA84697.1 .
RefSeqi NP_067498.4. NM_021523.4.
UniGenei Mm.27372.
Mm.488413.

3D structure databases

ProteinModelPortali Q7TMY8.
SMRi Q7TMY8. Positions 1310-1356, 4003-4369.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 208493. 9 interactions.
IntActi Q7TMY8. 3 interactions.
MINTi MINT-4139570.
STRINGi 10090.ENSMUSP00000026292.

PTM databases

PhosphoSitei Q7TMY8.

Proteomic databases

MaxQBi Q7TMY8.
PaxDbi Q7TMY8.
PRIDEi Q7TMY8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000112622 ; ENSMUSP00000108241 ; ENSMUSG00000025261 . [Q7TMY8-1 ]
GeneIDi 59026.
KEGGi mmu:59026.
UCSCi uc009upq.2. mouse. [Q7TMY8-1 ]
uc009upr.1. mouse. [Q7TMY8-2 ]

Organism-specific databases

CTDi 10075.
MGIi MGI:1926884. Huwe1.
Rougei Search...

Phylogenomic databases

eggNOGi COG5021.
GeneTreei ENSGT00760000118966.
HOVERGENi HBG080254.
InParanoidi Q7TMY8.
KOi K10592.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSi Huwe1. mouse.
NextBioi 314602.
PROi Q7TMY8.
SOURCEi Search...

Gene expression databases

Bgeei Q7TMY8.
CleanExi MM_HUWE1.
ExpressionAtlasi Q7TMY8. baseline and differential.
Genevestigatori Q7TMY8.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR025527. DUF4414.
IPR010309. E3_Ub_ligase_DUF908.
IPR010314. E3_Ub_ligase_DUF913.
IPR000569. HECT.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR004170. WWE-dom.
[Graphical view ]
Pfami PF14377. DUF4414. 1 hit.
PF06012. DUF908. 1 hit.
PF06025. DUF913. 1 hit.
PF00632. HECT. 1 hit.
PF00627. UBA. 1 hit.
PF02825. WWE. 1 hit.
[Graphical view ]
SMARTi SM00119. HECTc. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view ]
SUPFAMi SSF46934. SSF46934. 1 hit.
SSF48371. SSF48371. 3 hits.
SSF56204. SSF56204. 1 hit.
PROSITEi PS50237. HECT. 1 hit.
PS50030. UBA. 1 hit.
PS50918. WWE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor."
    Chen D., Kon N., Li M., Zhang W., Qin J., Gu W.
    Cell 121:1071-1083(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
  2. Chen D., Kon N., Li M., Zhang W., Qin J., Gu W.
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 253; 1728; 2009-2010; 2032 AND 3034-3035.
  3. "Characterization of E3Histone, a novel testis ubiquitin protein ligase which ubiquitinates histones."
    Liu Z., Oughtred R., Wing S.S.
    Mol. Cell. Biol. 25:2819-2831(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
    Strain: C57BL/6J.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
    DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1445-4377 (ISOFORM 1).
    Tissue: Embryonic intestine.
  6. Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
    Tissue: Brain.
  7. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1627-1655; 3127-3133 AND 3245-3253, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1630-4377 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3283-4377 (ISOFORM 3).
    Strain: C57BL/6 and FVB/N-3.
    Tissue: Brain, Eye and Mammary gland.
  9. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3421-4377 (ISOFORM 4).
    Strain: C57BL/6J.
  10. "Mouse homolog to KIAA0312."
    Seki N., Hattori A., Hayashi A., Kozuma S., Muramatsu M., Saito T.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4005-4377 (ISOFORMS 1/3).
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  12. "Regulated expression of the ubiquitin protein ligase, E3(Histone)/LASU1/Mule/ARF-BP1/HUWE1, during spermatogenesis."
    Liu Z., Miao D., Xia Q., Hermo L., Wing S.S.
    Dev. Dyn. 236:2889-2898(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  13. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1395; SER-1907 AND SER-3810, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  14. "The HECT-domain ubiquitin ligase Huwe1 controls neural differentiation and proliferation by destabilizing the N-Myc oncoprotein."
    Zhao X., Heng J.I.-T., Guardavaccaro D., Jiang R., Pagano M., Guillemot F., Iavarone A., Lasorella A.
    Nat. Cell Biol. 10:643-653(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  15. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1907, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  16. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-2267, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiHUWE1_MOUSE
AccessioniPrimary (citable) accession number: Q7TMY8
Secondary accession number(s): A2AFQ1
, Q4G2Z1, Q5BMM7, Q6NS61, Q8BNJ7, Q8CFH2, Q8VD14, Q921M5, Q9R0P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 105 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3