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Q7TMY8

- HUWE1_MOUSE

UniProt

Q7TMY8 - HUWE1_MOUSE

Protein

E3 ubiquitin-protein ligase HUWE1

Gene

Huwe1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 5 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene By similarity. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei4344 – 43441Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB
    2. ligase activity Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. base-excision repair Source: UniProtKB
    2. cell differentiation Source: UniProtKB-KW
    3. histone ubiquitination Source: UniProtKB
    4. protein monoubiquitination Source: UniProtKB
    5. protein polyubiquitination Source: UniProtKB
    6. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: RefGenome

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Differentiation, DNA damage, DNA repair, Ubl conjugation pathway

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase HUWE1 (EC:6.3.2.-)
    Alternative name(s):
    E3Histone
    HECT, UBA and WWE domain-containing protein 1
    Upstream regulatory element-binding protein 1
    Short name:
    URE-B1
    Short name:
    URE-binding protein 1
    Gene namesi
    Name:Huwe1
    Synonyms:Kiaa0312, Ureb1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1926884. Huwe1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 43774377E3 ubiquitin-protein ligase HUWE1PRO_0000120341Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1084 – 10841PhosphoserineBy similarity
    Modified residuei1368 – 13681PhosphoserineBy similarity
    Modified residuei1370 – 13701PhosphoserineBy similarity
    Modified residuei1395 – 13951Phosphoserine1 Publication
    Modified residuei1907 – 19071Phosphoserine2 Publications
    Modified residuei2267 – 22671N6-acetyllysine1 Publication
    Modified residuei2362 – 23621PhosphoserineBy similarity
    Modified residuei2365 – 23651PhosphoserineBy similarity
    Modified residuei2391 – 23911PhosphoserineBy similarity
    Modified residuei2595 – 25951PhosphoserineBy similarity
    Modified residuei2619 – 26191PhosphoserineBy similarity
    Modified residuei2751 – 27511PhosphothreonineBy similarity
    Modified residuei2887 – 28871PhosphoserineBy similarity
    Modified residuei2918 – 29181PhosphoserineBy similarity
    Modified residuei3116 – 31161PhosphoserineBy similarity
    Modified residuei3127 – 31271PhosphoserineBy similarity
    Modified residuei3663 – 36631PhosphoserineBy similarity
    Modified residuei3753 – 37531PhosphoserineBy similarity
    Modified residuei3758 – 37581PhosphoserineBy similarity
    Modified residuei3761 – 37611PhosphoserineBy similarity
    Modified residuei3810 – 38101Phosphoserine1 Publication
    Modified residuei3818 – 38181PhosphoserineBy similarity
    Modified residuei3922 – 39221PhosphoserineBy similarity
    Modified residuei3927 – 39271PhosphothreonineBy similarity
    Modified residuei3930 – 39301PhosphothreonineBy similarity

    Post-translational modificationi

    Phosphorylated on tyrosine; phosphorylation is probably required for its ability to inhibit TP53 transactivation.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ7TMY8.
    PaxDbiQ7TMY8.
    PRIDEiQ7TMY8.

    PTM databases

    PhosphoSiteiQ7TMY8.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Developmental stagei

    Expression increases during neuronal differentiation such that the cortical plate contains the highest level.1 Publication

    Gene expression databases

    ArrayExpressiQ7TMY8.
    BgeeiQ7TMY8.
    CleanExiMM_HUWE1.
    GenevestigatoriQ7TMY8.

    Interactioni

    Subunit structurei

    Interacts with isoform p19ARF of CDKN2A which strongly inhibits HUWE1 ubiquitin ligase activity. Interacts with MYCN, POLB and CDC6.By similarity

    Protein-protein interaction databases

    BioGridi208493. 9 interactions.
    IntActiQ7TMY8. 3 interactions.
    MINTiMINT-4139570.
    STRINGi10090.ENSMUSP00000026292.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7TMY8.
    SMRiQ7TMY8. Positions 1310-1356, 4003-4369.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1316 – 135540UBAPROSITE-ProRule annotationAdd
    BLAST
    Repeati1370 – 138920UIMAdd
    BLAST
    Domaini1603 – 168078WWEPROSITE-ProRule annotationAdd
    BLAST
    Domaini4041 – 4377337HECTPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi2295 – 2469175Glu-richAdd
    BLAST
    Compositional biasi2427 – 249064Asp-richAdd
    BLAST
    Compositional biasi3484 – 355269Thr-richAdd
    BLAST

    Domaini

    The HECT domain mediates inhibition of the transcriptional activity of p53.By similarity

    Sequence similaritiesi

    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation
    Contains 1 UBA domain.PROSITE-ProRule annotation
    Contains 1 WWE domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5021.
    GeneTreeiENSGT00750000117291.
    HOVERGENiHBG080254.
    KOiK10592.

    Family and domain databases

    InterProiIPR016024. ARM-type_fold.
    IPR025527. DUF4414.
    IPR010309. E3_Ub_ligase_DUF908.
    IPR010314. E3_Ub_ligase_DUF913.
    IPR000569. HECT.
    IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR004170. WWE-dom.
    [Graphical view]
    PfamiPF14377. DUF4414. 1 hit.
    PF06012. DUF908. 1 hit.
    PF06025. DUF913. 1 hit.
    PF00632. HECT. 1 hit.
    PF00627. UBA. 1 hit.
    PF02825. WWE. 1 hit.
    [Graphical view]
    SMARTiSM00119. HECTc. 1 hit.
    SM00165. UBA. 1 hit.
    [Graphical view]
    SUPFAMiSSF46934. SSF46934. 1 hit.
    SSF48371. SSF48371. 3 hits.
    SSF56204. SSF56204. 1 hit.
    PROSITEiPS50237. HECT. 1 hit.
    PS50030. UBA. 1 hit.
    PS50918. WWE. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q7TMY8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKVDRTKLKK TPTEAPADCR ALIDKLKVCN DEQLLLELQQ IKTWNIGKCE     50
    LYHWVDLLDR FDGILADAGQ TVENMSWMLV CDRPEKEQLK MLLLAVLNFT 100
    ALLIEYSFSR HLYSSIEHLT TLLASSDMQV VLAVLNLLYV FSKRSNYITR 150
    LGSDKRTPLL TRLQHLAESW GGKENGFGLA ECCRDLQMLK YPPSATTLHF 200
    EFYADPGAEV KIEKRTTSNT LHYIHIEQLD KISESPSEIM ESLTKMYSIP 250
    KDKQMLLFTH IRLAHGFSNH RKRLQAVQAR LHAISILVYS NALQESANSI 300
    LYNGLIEELV DVLQITDKQL MEIKAASLRT LTSIVHLERT PKLSSIIDCT 350
    GTASYHGFLP VLVRNCIQAM IDPSMDPYPH QFATALFSFL YHLASYDAGG 400
    EALVSCGMME ALLKVIKFLG DEQDQITFVT RAVRVVDLIT NLDMAAFQSH 450
    SGLSIFIYRL EHEVDLCRKE CPFVIKPKIQ RPSTTQEGEE METDMDGVQC 500
    IPQRAALLKS MLNFLKKAIQ DPAFSDGIRH VMDGSLPTSL KHIISNAEYY 550
    GPSLFLLATE VVTVFVFQEP SLLSSLQDNG LTDVMLHALL IKDVPATREV 600
    LGSLPNVFSA LCLNARGLQS FVQCQPFERL FKVLLSPDYL PAMRRRRSSD 650
    PLGDTASNLG SAVDELMRHQ PTLKTDATTA IIKLLEEICN LGRDPKYICQ 700
    KPSIQKADGT ATAPPPRSNH AAEEASSEDE EEEEVQAMQS FNSAQQNETE 750
    PNQQVVGTEE RIPIPLMDYI LNVMKFVESI LSNNTTDDHC QEFVNQKGLL 800
    PLVTILGLPN LPIDFPTSAA CQAVAGVCKS ILTLSHEPKV LQEGLLQLDL 850
    ILSSLEPLHR PIESPGGSVL LRELACAGNV ADATLSAQAT PLLHALTAAH 900
    AYIMMFVHTC RVGQSEIRSI SVNQWGSQLG LSVLSKLSQL YCSLVWESTV 950
    LLSLCTPNSL PSGCEFGQAD MQKLVPKDEK AGTTQGGKRS DGEQDGTAGS 1000
    MDASAQGLLE GIELDGDTLA PMETDEPSSS DSKGKSKITP AMAARIKQIK 1050
    PLLSASSRLG RALAELFGLL VKLCVGSPVR QRRSHHAAST TTAPTPAARS 1100
    TASALTKLLT KGLSWQPPPY TPTPRFRLTF FICSVGFTSP MLFDERKYPY 1150
    HLMLQKFLCS GGHNALFETF NWALSMGGKV PVSEGLEHSD LPDGTGEFLD 1200
    AWLMLVEKMV NPTTVLESPH SLPAKLPGGV QSFPQFSALR FLVVTQKAAF 1250
    TCIKNLWNRK PLKVYGGRMA ESMLAILCHI LRGEPVIRER LSKEKEGSRG 1300
    EEEAGQEEGG SRREPQVNQQ QLQQLMDMGF TREHAMEALL NTSTMEQATE 1350
    YLLTHPPPII GGVVRDLSMS EEDQMMRAIA MSLGQDIPMD QRAESPEEVA 1400
    CRKEEEERKA REKQEEEEAK CLEKFQDADP LEQDELHTFT DTMLPGCFHL 1450
    LDELPDTVYR VCDLIMTAIK RNGADYRDMI LKQVVNQVWE AADVLIKAAL 1500
    PLTTSDTKTV SEWISQMATL PQASNLATRI LLLTLLFEEL KLPCAWVVES 1550
    SGILNVLIKL LEVVQPCLQA AKEQKEVQTP KWITPVLLLI DFYEKTAISS 1600
    KRRAQMTKYL QSNSNNWRWF DDRSGRWCSY SASNNSTIDS AWKSGETSVR 1650
    FTAGRRRYTV QFTTMVQVNE ETGNRRPVML TLLRVPRLSK NSKSSNGQEL 1700
    EKTLEESKET DIKHKENKGN DIPLALESTN TEKEASLDET KIGEILIQGL 1750
    TEDMVTVLIR ACVSMLGVPV DPDTLHATLR LCLRLTRDHK YAMMFAELKS 1800
    TRMILNLTQS SGFNGFTPLV TLLLRHIIED PCTLRHTMEK VVRSAATSGA 1850
    GSTTSGVVSG SLGSREINYI LRVLGPAACR NPDIFTEVAN CCIRIALPAP 1900
    RGSGTASDDE FENLRIKGPN AVQLVKTTPL KPSSLPVIPD TIKEVIYDML 1950
    NALAAYHAPE EADKSDPKPG GTTQEVGQLL QDMGDDVYQQ YRSLTRQSSD 2000
    FDTQSGFSLN SQVFAADGAP AETSTTGTSQ GEASTPEETR EGKKDKEGDR 2050
    TSEEGKQKSK GSKPLMPTST ILRLLAELVR SYVGIATLIA NYSYTVGQSE 2100
    LIKEDCSVLA FVLDHLLPHT QNAEDKDTPA LARLFLASLA AAGSGTDAQV 2150
    ALVNEVKAAL GRALAMAEST EKHARLQAVM CIISTIMESC PSTSSFYSSA 2200
    TAKTQHNGMN NIIRLFLKKG LVNDLARVPH SLDLSSPNMA NTVNAALKPL 2250
    ETLSRIVNQP SSLFGSKSAS SKNKSEQDAQ GASQDSSSHQ QDPGEPGEAE 2300
    VQEEDHDVTQ TEVADGDIMD GEAETDSVVI AGQPEVLSSQ EMQVENELED 2350
    LIDELLERDG GSGNSTIIVS RSGEDESQED VLMDEAPSNL SQASTLQANR 2400
    EDSMNILDPE DEEEHTQEED SSGSNEDEDD SQDEEEEEEE DEEDDQEDDE 2450
    GEEGDEDDDD DGSEMELDED YPDMNASPLV RFERFDREDD LIIEFDNMFS 2500
    SATDIPPSPG NIPTTHPLMV RHADHSSLTL GSGSSTTRLT QGIGRSQRTL 2550
    RQLTANTGHT IHVHYPGNRQ PNPPLILQRL LGPSAAADIL QLSSSLPLQS 2600
    RGRARLLVGN DDVHIIARSD DELLDDFFHD QSTATSQAGT LSSIPTALTR 2650
    WTEECKVLDA ESMHDCVSVV KVPIVNHLEF LRDEELEERR EKRRKQLAEE 2700
    ETKIIDKGKE DKENRDQSAQ CTVTKTNDST EQNVSDGTPM PDSYPTTPSS 2750
    TDAPTSESKE TLGTLQPSQQ QPALPPPPSL GEIPQELQSP AEEVANSTQL 2800
    LMPIELEELG PTRPSGEAET TQMELSPAPT ITSLSPERAE DSDALTAVSS 2850
    QLEGSPMDTS SLASCTLEEA VGDTPAAGSS EQPTAGSSTP GDAPSVVAEV 2900
    QGRPDVSRES NQPPEDSSPP ASSESSSTRD SAVAISGADS RGILEEPLPS 2950
    TSSEEEDPLA GISLPEGVDP SFLAALPDDI RREVLQNQLG IRPPTRSAPS 3000
    SNSSAPAVVG NPGVTEVSPE FLAALPPAIQ EEVLAQQRAE QQRRELAQNA 3050
    SSDTPMDPVT FIQTLPSDLR RSVLEDMEDS VLAVMPPDIA AEAQALRREQ 3100
    EARQRQLMHE RLFGHSSTSA LSAILRSPAF TSRLSGNRGV QYTRLAVQRG 3150
    GTFQMGGSSS HNRPSGSNVD TLLRLRGRLL LDHEALSCLL VLLFVDEPKL 3200
    NTSRLHRVLR NLCYHAQTRH WVIRSLLSIL QRSSESELCI ETPKLSTSEE 3250
    RGKKSSKSCA SSSHENRPLD LLHKMESKSS NQLSWLSVSM DAALGCRTNI 3300
    FQIQRSGGRK HTEKHASSGS TVHIHPQAAP VVCRHVLDTL IQLAKVFPSH 3350
    FTQQRTKETN CESDRERGSK QACSPCSSQS SSSGICTDFW DLLVKLDNMN 3400
    VSRKGKNSVK SVPVSSGGEG ETSPHSLEAS PLGQLMNMLS HPVIRRSSLL 3450
    TEKLLRLLSL ISIALPENKV SEVQTNSSNS GSSTAATSNT STTTTTTTTA 3500
    TAPTPTPPAA TTPVTSAPAL VAATAISTIT VAASTTVTTP TTATTTVSTS 3550
    TTKGSKSPAK VGEGGSGIDF KMVSSGLTEN QLQLSVEVLT SHSCSEEGLE 3600
    DAANVLLQLS RGDSGTRDTV LKLLLNGARH LGYTLCKQIG TLLAELREYN 3650
    LEQQRRAQCE TLSPDGLPEE QPQTTKLKGK MQSRFDMAEN VVIVASQKRP 3700
    LGGRELQLPS MSMLTSKTST QKFFLRVLQV IIQLRDDTRR ANKKAKQTGR 3750
    LGSSGLGSAS SIQAAVRQLE AEADAIIQMV REGQRARRQQ QAATSESSNQ 3800
    SETSVRREES PMDVDQPSPS AQDTQSIVIS DGTPQGEKEK EEKPPELPLL 3850
    SEQLSLDELW DMLGECLKEL EESHDQHAVL VLQPAVEAFF LVHATERESK 3900
    PPVRDTRESQ LAHIKDEPPP LSPAPLTPAT PSSLDPFFSR EPSSMHISSS 3950
    LPPDTQKFLR FAETHRTVLN QILRQSTTHL ADGPFAVLVD YIRVLDFDVK 4000
    RKYFRQELER LDEGLRKEDM AVHVRRDHVF EDSYRELHRK SPEEMKNRLY 4050
    IVFEGEEGQD AGGLLREWYM IISREMFNPM YALFRTSPGD RVTYTINPSS 4100
    HCNPNHLSYF KFVGRIVAKA VYDNRLLECY FTRSFYKHIL GKSVRYTDME 4150
    SEDYHFYQGL VYLLENDVST LGYDLTFSTE VQEFGVCEVR DLKPNGANIL 4200
    VTEENKKEYV HLVCQMRMTG AIRKQLAAFL EGFYEIIPKR LISIFTEQEL 4250
    ELLISGLPTI DIDDLKSNTE YHKYQSNSIQ IQWFWRALRS FDQADRAKFL 4300
    QFVTGTSKVP LQGFAALEGM NGIQKFQIHR DDRSTDRLPS AHTCFNQLDL 4350
    PAYESFEKLR HMLLLAIQEC SEGFGLA 4377
    Length:4,377
    Mass (Da):482,635
    Last modified:July 27, 2011 - v5
    Checksum:i22700C859CD9AF83
    GO
    Isoform 2 (identifier: Q7TMY8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         3347-3364: FPSHFTQQRTKETNCESD → RSLKESLTPGFFGHQHLG
         3365-4377: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:3,364
    Mass (Da):369,701
    Checksum:iBD5116429BF0E509
    GO
    Isoform 3 (identifier: Q7TMY8-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         3780-3794: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:4,362
    Mass (Da):480,898
    Checksum:i0D640BFE13725964
    GO
    Isoform 4 (identifier: Q7TMY8-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         3795-3832: SESSNQSETS...DTQSIVISDG → VSMMPVAPHS...LVCFCITFAK
         3833-4377: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:3,832
    Mass (Da):419,496
    Checksum:iC8B75208FD6CB0EC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti253 – 2531K → R in AAX24124. (PubMed:15767685)Curated
    Sequence conflicti253 – 2531K → R in AAV90839. (PubMed:15989956)Curated
    Sequence conflicti1728 – 17281S → I in AAV90839. (PubMed:15989956)Curated
    Sequence conflicti1728 – 17281S → I in BAC41411. (PubMed:12465718)Curated
    Sequence conflicti2032 – 20321E → EG in AAV90839. (PubMed:15989956)Curated
    Sequence conflicti2032 – 20321E → EG in BAC41411. (PubMed:12465718)Curated
    Sequence conflicti2032 – 20321E → EG in AAH70444. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei3347 – 336418FPSHF…NCESD → RSLKESLTPGFFGHQHLG in isoform 2. 1 PublicationVSP_011147Add
    BLAST
    Alternative sequencei3365 – 43771013Missing in isoform 2. 1 PublicationVSP_011148Add
    BLAST
    Alternative sequencei3780 – 379415Missing in isoform 3. 1 PublicationVSP_011149Add
    BLAST
    Alternative sequencei3795 – 383238SESSN…VISDG → VSMMPVAPHSFLYPPSCTMS SVGVHCPYLVCFCITFAK in isoform 4. 1 PublicationVSP_011150Add
    BLAST
    Alternative sequencei3833 – 4377545Missing in isoform 4. 1 PublicationVSP_011151Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY772010 mRNA. Translation: AAV90839.3.
    AY929611 mRNA. Translation: AAX24124.1.
    BX571795, AL672180, AL954855 Genomic DNA. Translation: CAM13774.1.
    AL954855, AL672180, BX571795 Genomic DNA. Translation: CAM17616.1.
    AL672180, AL954855, BX571795 Genomic DNA. Translation: CAM23826.1.
    AB093227 mRNA. Translation: BAC41411.2.
    BC011391 mRNA. Translation: AAH11391.1.
    BC017642 mRNA. Translation: AAH17642.2.
    BC054372 mRNA. Translation: AAH54372.1.
    BC070444 mRNA. Translation: AAH70444.1.
    AK083499 mRNA. Translation: BAC38936.1.
    AB025966 mRNA. Translation: BAA84697.1.
    RefSeqiNP_067498.4. NM_021523.4.
    UniGeneiMm.27372.
    Mm.488413.

    Genome annotation databases

    EnsembliENSMUST00000112622; ENSMUSP00000108241; ENSMUSG00000025261. [Q7TMY8-1]
    GeneIDi59026.
    KEGGimmu:59026.
    UCSCiuc009upq.2. mouse. [Q7TMY8-1]
    uc009upr.1. mouse. [Q7TMY8-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY772010 mRNA. Translation: AAV90839.3 .
    AY929611 mRNA. Translation: AAX24124.1 .
    BX571795 , AL672180 , AL954855 Genomic DNA. Translation: CAM13774.1 .
    AL954855 , AL672180 , BX571795 Genomic DNA. Translation: CAM17616.1 .
    AL672180 , AL954855 , BX571795 Genomic DNA. Translation: CAM23826.1 .
    AB093227 mRNA. Translation: BAC41411.2 .
    BC011391 mRNA. Translation: AAH11391.1 .
    BC017642 mRNA. Translation: AAH17642.2 .
    BC054372 mRNA. Translation: AAH54372.1 .
    BC070444 mRNA. Translation: AAH70444.1 .
    AK083499 mRNA. Translation: BAC38936.1 .
    AB025966 mRNA. Translation: BAA84697.1 .
    RefSeqi NP_067498.4. NM_021523.4.
    UniGenei Mm.27372.
    Mm.488413.

    3D structure databases

    ProteinModelPortali Q7TMY8.
    SMRi Q7TMY8. Positions 1310-1356, 4003-4369.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 208493. 9 interactions.
    IntActi Q7TMY8. 3 interactions.
    MINTi MINT-4139570.
    STRINGi 10090.ENSMUSP00000026292.

    PTM databases

    PhosphoSitei Q7TMY8.

    Proteomic databases

    MaxQBi Q7TMY8.
    PaxDbi Q7TMY8.
    PRIDEi Q7TMY8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000112622 ; ENSMUSP00000108241 ; ENSMUSG00000025261 . [Q7TMY8-1 ]
    GeneIDi 59026.
    KEGGi mmu:59026.
    UCSCi uc009upq.2. mouse. [Q7TMY8-1 ]
    uc009upr.1. mouse. [Q7TMY8-2 ]

    Organism-specific databases

    CTDi 10075.
    MGIi MGI:1926884. Huwe1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG5021.
    GeneTreei ENSGT00750000117291.
    HOVERGENi HBG080254.
    KOi K10592.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.

    Miscellaneous databases

    ChiTaRSi HUWE1. mouse.
    NextBioi 314602.
    PROi Q7TMY8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q7TMY8.
    Bgeei Q7TMY8.
    CleanExi MM_HUWE1.
    Genevestigatori Q7TMY8.

    Family and domain databases

    InterProi IPR016024. ARM-type_fold.
    IPR025527. DUF4414.
    IPR010309. E3_Ub_ligase_DUF908.
    IPR010314. E3_Ub_ligase_DUF913.
    IPR000569. HECT.
    IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR004170. WWE-dom.
    [Graphical view ]
    Pfami PF14377. DUF4414. 1 hit.
    PF06012. DUF908. 1 hit.
    PF06025. DUF913. 1 hit.
    PF00632. HECT. 1 hit.
    PF00627. UBA. 1 hit.
    PF02825. WWE. 1 hit.
    [Graphical view ]
    SMARTi SM00119. HECTc. 1 hit.
    SM00165. UBA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46934. SSF46934. 1 hit.
    SSF48371. SSF48371. 3 hits.
    SSF56204. SSF56204. 1 hit.
    PROSITEi PS50237. HECT. 1 hit.
    PS50030. UBA. 1 hit.
    PS50918. WWE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor."
      Chen D., Kon N., Li M., Zhang W., Qin J., Gu W.
      Cell 121:1071-1083(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
    2. Chen D., Kon N., Li M., Zhang W., Qin J., Gu W.
      Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 253; 1728; 2009-2010; 2032 AND 3034-3035.
    3. "Characterization of E3Histone, a novel testis ubiquitin protein ligase which ubiquitinates histones."
      Liu Z., Oughtred R., Wing S.S.
      Mol. Cell. Biol. 25:2819-2831(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
      Strain: C57BL/6J.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
      DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1445-4377 (ISOFORM 1).
      Tissue: Embryonic intestine.
    6. Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
      Tissue: Brain.
    7. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 1627-1655; 3127-3133 AND 3245-3253, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1630-4377 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3283-4377 (ISOFORM 3).
      Strain: C57BL/6 and FVB/N-3.
      Tissue: Brain, Eye and Mammary gland.
    9. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3421-4377 (ISOFORM 4).
      Strain: C57BL/6J.
    10. "Mouse homolog to KIAA0312."
      Seki N., Hattori A., Hayashi A., Kozuma S., Muramatsu M., Saito T.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4005-4377 (ISOFORMS 1/3).
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    12. "Regulated expression of the ubiquitin protein ligase, E3(Histone)/LASU1/Mule/ARF-BP1/HUWE1, during spermatogenesis."
      Liu Z., Miao D., Xia Q., Hermo L., Wing S.S.
      Dev. Dyn. 236:2889-2898(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    13. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1395; SER-1907 AND SER-3810, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    14. "The HECT-domain ubiquitin ligase Huwe1 controls neural differentiation and proliferation by destabilizing the N-Myc oncoprotein."
      Zhao X., Heng J.I.-T., Guardavaccaro D., Jiang R., Pagano M., Guillemot F., Iavarone A., Lasorella A.
      Nat. Cell Biol. 10:643-653(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE.
    15. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1907, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    16. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-2267, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiHUWE1_MOUSE
    AccessioniPrimary (citable) accession number: Q7TMY8
    Secondary accession number(s): A2AFQ1
    , Q4G2Z1, Q5BMM7, Q6NS61, Q8BNJ7, Q8CFH2, Q8VD14, Q921M5, Q9R0P2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 103 of the entry and version 5 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3