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Protein

Importin-8

Gene

Ipo8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Seems to function in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro mediates the nuclear import of SRP19 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-5578749. Transcriptional regulation by small RNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin-8
Short name:
Imp8
Alternative name(s):
Ran-binding protein 8
Short name:
RanBP8
Gene namesi
Name:Ipo8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2444611. Ipo8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10101010Importin-8PRO_0000120753Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei902 – 9021PhosphoserineBy similarity
Modified residuei903 – 9031PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7TMY7.
MaxQBiQ7TMY7.
PaxDbiQ7TMY7.
PRIDEiQ7TMY7.

PTM databases

iPTMnetiQ7TMY7.
PhosphoSiteiQ7TMY7.

Expressioni

Gene expression databases

BgeeiQ7TMY7.
CleanExiMM_IPO8.
ExpressionAtlasiQ7TMY7. baseline and differential.
GenevisibleiQ7TMY7. MM.

Interactioni

Subunit structurei

Forms a heterodimer with KPNB1. Interacts with SRP19. Binds directly to nuclear pore complexes (By similarity).By similarity

Protein-protein interaction databases

IntActiQ7TMY7. 2 interactions.
STRINGi10090.ENSMUSP00000046759.

Structurei

3D structure databases

ProteinModelPortaliQ7TMY7.
SMRiQ7TMY7. Positions 131-156, 461-527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 10281Importin N-terminalPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi927 – 9326Poly-Asp

Sequence similaritiesi

Belongs to the importin beta family.Curated
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1991. Eukaryota.
COG5656. LUCA.
GeneTreeiENSGT00550000074736.
HOGENOMiHOG000006586.
HOVERGENiHBG006824.
InParanoidiQ7TMY7.
KOiK18755.
OMAiQTQAKEY.
OrthoDBiEOG7TF787.
TreeFamiTF300634.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013713. Cse1.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF08506. Cse1. 1 hit.
PF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TMY7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLNRIIQAL KGTIDPKLRI AAETELNQSY KIINFAPSLL RIIVSDHVEF
60 70 80 90 100
PVRQAAAIYL KNMVTQYWPD REPPPGEVIF PFNIHENDRQ QIRDNIVEGI
110 120 130 140 150
IRSPDLVRVQ LTMCLRVIIR HDFPGHWPAV VDKIDYYLQS PNSGSWLGSL
160 170 180 190 200
LCLYQLVKTY EYKKAEEREP LLAAMQIFLP RIQQQILQLL PDASHYSVLL
210 220 230 240 250
QKQILKIFYA LVQYALPLQL VNHQTMTTWM EIFRTIIDRT VPPETLQIDE
260 270 280 290 300
DDRPELVWWK CKKWALHIVA RLFERYGSPG NVTKEYFEFS EFFLKTYAVG
310 320 330 340 350
IQQVLLKILD QYRQKEYIAP RVLQQAFNYL NQGVVHAVTW KQMKPHIQNI
360 370 380 390 400
SEDVIFSVMC YKDEDEELWQ EDPYEYIRMK FDIFEDYASP TTAAQTLLYT
410 420 430 440 450
AAKKRKEVLP KMMAFCYQIL TDPNFDPRKK DGALHVIGSL AEILLKKSLF
460 470 480 490 500
KDQIELFLQN HVFPLLMSNL GYLRARSCWV LHAFSSLKFH NELNLRNAVE
510 520 530 540 550
LAKKSLIEDE EMPVKVEAAL ALQSLISNQA QAKEHMKPYV RFIMQELLHI
560 570 580 590 600
VRETENDDVT NVIQKLICEY SQDVASIAVD TTQHLAEIFG KVLQSDEYEE
610 620 630 640 650
IEDKTVMAMG ILHTIDTILT VVEDHPEIIQ QLENICLRII DLVLQKHVIE
660 670 680 690 700
FYEEILSLAY NLTCHTISPQ MWQLLGILYE VFQQDCFEYF TDMMPLLHNY
710 720 730 740 750
VTVDTNALLS NPKHLEVLFT MCRKVLCGEA GEDAECYAAK LLEVIILQCK
760 770 780 790 800
GRGIDQCIPL FIQLVLERLT RGVKTSELRT MCLQVAIAAL YYSPELLFHT
810 820 830 840 850
LEQVQLPHNP GPVTSQFINQ WMNDTDYFLG HHDRKMCIIG LSVLLELQNR
860 870 880 890 900
PPAVDAVAAQ ILPSILFLFL GLKQVCATRQ TVNRENHSKA EKVDIEENEE
910 920 930 940 950
ISSEEEEETS VSAQAMQSQI GRSEEEDDDD WDEEVLEETA LEGFSTPLDL
960 970 980 990 1000
DNSVDEYQFF TQALLTVQNR DAAWYQLLVA PLSEDQKRKL QEVYTLAEHR
1010
RTLAAGQFHI
Length:1,010
Mass (Da):117,078
Last modified:July 27, 2011 - v3
Checksum:i56BFECF32695BD7A
GO
Isoform 2 (identifier: Q7TMY7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     304-347: Missing.

Note: No experimental confirmation available.
Show »
Length:966
Mass (Da):111,817
Checksum:i9F52A7592E3FBD8E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti405 – 4051R → K in AAH44819 (PubMed:15489334).Curated
Sequence conflicti405 – 4051R → K in AAH54373 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei304 – 34744Missing in isoform 2. 1 PublicationVSP_009656Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU302294 Genomic DNA. Translation: CAQ52306.1.
CH466572 Genomic DNA. Translation: EDL10748.1.
BC024474 mRNA. Translation: AAH24474.1.
BC027360 mRNA. Translation: AAH27360.1.
BC044819 mRNA. Translation: AAH44819.1.
BC054373 mRNA. Translation: AAH54373.1.
BC151036 mRNA. Translation: AAI51037.1.
BC151052 mRNA. Translation: AAI51053.1.
AK047854 mRNA. Translation: BAC33175.1.
CD350256 mRNA. No translation available.
BQ770006 mRNA. No translation available.
CCDSiCCDS39719.1. [Q7TMY7-1]
RefSeqiNP_001074582.1. NM_001081113.1. [Q7TMY7-1]
UniGeneiMm.260105.

Genome annotation databases

EnsembliENSMUST00000048418; ENSMUSP00000046759; ENSMUSG00000040029. [Q7TMY7-1]
GeneIDi320727.
KEGGimmu:320727.
UCSCiuc009etl.1. mouse. [Q7TMY7-1]
uc009etn.2. mouse. [Q7TMY7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU302294 Genomic DNA. Translation: CAQ52306.1.
CH466572 Genomic DNA. Translation: EDL10748.1.
BC024474 mRNA. Translation: AAH24474.1.
BC027360 mRNA. Translation: AAH27360.1.
BC044819 mRNA. Translation: AAH44819.1.
BC054373 mRNA. Translation: AAH54373.1.
BC151036 mRNA. Translation: AAI51037.1.
BC151052 mRNA. Translation: AAI51053.1.
AK047854 mRNA. Translation: BAC33175.1.
CD350256 mRNA. No translation available.
BQ770006 mRNA. No translation available.
CCDSiCCDS39719.1. [Q7TMY7-1]
RefSeqiNP_001074582.1. NM_001081113.1. [Q7TMY7-1]
UniGeneiMm.260105.

3D structure databases

ProteinModelPortaliQ7TMY7.
SMRiQ7TMY7. Positions 131-156, 461-527.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7TMY7. 2 interactions.
STRINGi10090.ENSMUSP00000046759.

PTM databases

iPTMnetiQ7TMY7.
PhosphoSiteiQ7TMY7.

Proteomic databases

EPDiQ7TMY7.
MaxQBiQ7TMY7.
PaxDbiQ7TMY7.
PRIDEiQ7TMY7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048418; ENSMUSP00000046759; ENSMUSG00000040029. [Q7TMY7-1]
GeneIDi320727.
KEGGimmu:320727.
UCSCiuc009etl.1. mouse. [Q7TMY7-1]
uc009etn.2. mouse. [Q7TMY7-2]

Organism-specific databases

CTDi10526.
MGIiMGI:2444611. Ipo8.

Phylogenomic databases

eggNOGiKOG1991. Eukaryota.
COG5656. LUCA.
GeneTreeiENSGT00550000074736.
HOGENOMiHOG000006586.
HOVERGENiHBG006824.
InParanoidiQ7TMY7.
KOiK18755.
OMAiQTQAKEY.
OrthoDBiEOG7TF787.
TreeFamiTF300634.

Enzyme and pathway databases

ReactomeiR-MMU-5578749. Transcriptional regulation by small RNAs.

Miscellaneous databases

PROiQ7TMY7.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TMY7.
CleanExiMM_IPO8.
ExpressionAtlasiQ7TMY7. baseline and differential.
GenevisibleiQ7TMY7. MM.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR013713. Cse1.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF08506. Cse1. 1 hit.
PF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-406 AND 654-1010 (ISOFORMS 1 AND 2).
    Strain: FVB/N and FVB/N-3.
    Tissue: Brain and Mammary tumor.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 71-401 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Head.
  5. The MGC Project Team
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 407-653.
  6. Argoud-Puy G.
    Unpublished observations (JAN-2004)
    Cited for: RECONSTRUCTION FROM ESTS, CONCEPTUAL TRANSLATION OF 407-653.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Liver, Lung, Spleen and Testis.

Entry informationi

Entry nameiIPO8_MOUSE
AccessioniPrimary (citable) accession number: Q7TMY7
Secondary accession number(s): B2KGD8
, Q811I3, Q8C8A9, Q8R2P6, Q8R3V7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

The regions from 407 to 653 were deduced from the genomic sequence and ESTs by similarity to the human sequence.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.