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Protein

Importin-8

Gene

Ipo8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Seems to function in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro mediates the nuclear import of SRP19 (By similarity).By similarity

Caution

The regions from 407 to 653 were deduced from the genomic sequence and ESTs by similarity to the human sequence.Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-5578749 Transcriptional regulation by small RNAs

Names & Taxonomyi

Protein namesi
Recommended name:
Importin-8
Short name:
Imp8
Alternative name(s):
Ran-binding protein 8
Short name:
RanBP8
Gene namesi
Name:Ipo8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2444611 Ipo8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207531 – 1010Importin-8Add BLAST1010

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei902PhosphoserineBy similarity1
Modified residuei903PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7TMY7
PeptideAtlasiQ7TMY7
PRIDEiQ7TMY7

PTM databases

iPTMnetiQ7TMY7
PhosphoSitePlusiQ7TMY7

Expressioni

Gene expression databases

BgeeiENSMUSG00000040029
CleanExiMM_IPO8
ExpressionAtlasiQ7TMY7 baseline and differential
GenevisibleiQ7TMY7 MM

Interactioni

Subunit structurei

Forms a heterodimer with KPNB1. Interacts with SRP19. Binds directly to nuclear pore complexes (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ7TMY7, 2 interactors
STRINGi10090.ENSMUSP00000046759

Structurei

3D structure databases

ProteinModelPortaliQ7TMY7
SMRiQ7TMY7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 102Importin N-terminalPROSITE-ProRule annotationAdd BLAST81

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi927 – 932Poly-Asp6

Sequence similaritiesi

Belongs to the importin beta family.Curated

Phylogenomic databases

eggNOGiKOG1991 Eukaryota
COG5656 LUCA
GeneTreeiENSGT00550000074736
HOGENOMiHOG000006586
HOVERGENiHBG006824
InParanoidiQ7TMY7
KOiK18755
OMAiKDQMELL
OrthoDBiEOG091G01IF
TreeFamiTF300634

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR013713 Cse1
IPR001494 Importin-beta_N
PfamiView protein in Pfam
PF08506 Cse1, 1 hit
PF03810 IBN_N, 1 hit
SMARTiView protein in SMART
SM00913 IBN_N, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50166 IMPORTIN_B_NT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TMY7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLNRIIQAL KGTIDPKLRI AAETELNQSY KIINFAPSLL RIIVSDHVEF
60 70 80 90 100
PVRQAAAIYL KNMVTQYWPD REPPPGEVIF PFNIHENDRQ QIRDNIVEGI
110 120 130 140 150
IRSPDLVRVQ LTMCLRVIIR HDFPGHWPAV VDKIDYYLQS PNSGSWLGSL
160 170 180 190 200
LCLYQLVKTY EYKKAEEREP LLAAMQIFLP RIQQQILQLL PDASHYSVLL
210 220 230 240 250
QKQILKIFYA LVQYALPLQL VNHQTMTTWM EIFRTIIDRT VPPETLQIDE
260 270 280 290 300
DDRPELVWWK CKKWALHIVA RLFERYGSPG NVTKEYFEFS EFFLKTYAVG
310 320 330 340 350
IQQVLLKILD QYRQKEYIAP RVLQQAFNYL NQGVVHAVTW KQMKPHIQNI
360 370 380 390 400
SEDVIFSVMC YKDEDEELWQ EDPYEYIRMK FDIFEDYASP TTAAQTLLYT
410 420 430 440 450
AAKKRKEVLP KMMAFCYQIL TDPNFDPRKK DGALHVIGSL AEILLKKSLF
460 470 480 490 500
KDQIELFLQN HVFPLLMSNL GYLRARSCWV LHAFSSLKFH NELNLRNAVE
510 520 530 540 550
LAKKSLIEDE EMPVKVEAAL ALQSLISNQA QAKEHMKPYV RFIMQELLHI
560 570 580 590 600
VRETENDDVT NVIQKLICEY SQDVASIAVD TTQHLAEIFG KVLQSDEYEE
610 620 630 640 650
IEDKTVMAMG ILHTIDTILT VVEDHPEIIQ QLENICLRII DLVLQKHVIE
660 670 680 690 700
FYEEILSLAY NLTCHTISPQ MWQLLGILYE VFQQDCFEYF TDMMPLLHNY
710 720 730 740 750
VTVDTNALLS NPKHLEVLFT MCRKVLCGEA GEDAECYAAK LLEVIILQCK
760 770 780 790 800
GRGIDQCIPL FIQLVLERLT RGVKTSELRT MCLQVAIAAL YYSPELLFHT
810 820 830 840 850
LEQVQLPHNP GPVTSQFINQ WMNDTDYFLG HHDRKMCIIG LSVLLELQNR
860 870 880 890 900
PPAVDAVAAQ ILPSILFLFL GLKQVCATRQ TVNRENHSKA EKVDIEENEE
910 920 930 940 950
ISSEEEEETS VSAQAMQSQI GRSEEEDDDD WDEEVLEETA LEGFSTPLDL
960 970 980 990 1000
DNSVDEYQFF TQALLTVQNR DAAWYQLLVA PLSEDQKRKL QEVYTLAEHR
1010
RTLAAGQFHI
Length:1,010
Mass (Da):117,078
Last modified:July 27, 2011 - v3
Checksum:i56BFECF32695BD7A
GO
Isoform 2 (identifier: Q7TMY7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     304-347: Missing.

Note: No experimental confirmation available.
Show »
Length:966
Mass (Da):111,817
Checksum:i9F52A7592E3FBD8E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti405R → K in AAH44819 (PubMed:15489334).Curated1
Sequence conflicti405R → K in AAH54373 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009656304 – 347Missing in isoform 2. 1 PublicationAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU302294 Genomic DNA Translation: CAQ52306.1
CH466572 Genomic DNA Translation: EDL10748.1
BC024474 mRNA Translation: AAH24474.1
BC027360 mRNA Translation: AAH27360.1
BC044819 mRNA Translation: AAH44819.1
BC054373 mRNA Translation: AAH54373.1
BC151036 mRNA Translation: AAI51037.1
BC151052 mRNA Translation: AAI51053.1
AK047854 mRNA Translation: BAC33175.1
CD350256 mRNA No translation available.
BQ770006 mRNA No translation available.
CCDSiCCDS39719.1 [Q7TMY7-1]
RefSeqiNP_001074582.1, NM_001081113.1 [Q7TMY7-1]
UniGeneiMm.260105

Genome annotation databases

EnsembliENSMUST00000048418; ENSMUSP00000046759; ENSMUSG00000040029 [Q7TMY7-1]
GeneIDi320727
KEGGimmu:320727
UCSCiuc009etl.1 mouse [Q7TMY7-1]
uc009etn.2 mouse [Q7TMY7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiIPO8_MOUSE
AccessioniPrimary (citable) accession number: Q7TMY7
Secondary accession number(s): B2KGD8
, Q811I3, Q8C8A9, Q8R2P6, Q8R3V7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 130 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health