Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-binding cassette sub-family G member 2

Gene

Abcg2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-capacity urate exporter functioning in both renal and extrarenal urate excretion. Plays a role in porphyrin homeostasis as it is able to mediates the export of protoporhyrin IX (PPIX) both from mitochondria to cytosol and from cytosol to extracellular space, and cellular export of hemin, and heme. Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain (By similarity). May play a role in early stem cell self-renewal by blocking differentiation.By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi79 – 868ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2161517. Abacavir transmembrane transport.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-binding cassette sub-family G member 2
Alternative name(s):
Breast cancer resistance protein 1 homolog
Urate exporter
CD_antigen: CD338
Gene namesi
Name:Abcg2
Synonyms:Abcp, Bcrp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1347061. Abcg2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 393393CytoplasmicSequence analysisAdd
BLAST
Transmembranei394 – 41421HelicalSequence analysisAdd
BLAST
Topological domaini415 – 42814ExtracellularSequence analysisAdd
BLAST
Transmembranei429 – 44921HelicalSequence analysisAdd
BLAST
Topological domaini450 – 47728CytoplasmicSequence analysisAdd
BLAST
Transmembranei478 – 49821HelicalSequence analysisAdd
BLAST
Topological domaini499 – 5068ExtracellularSequence analysis
Transmembranei507 – 52721HelicalSequence analysisAdd
BLAST
Topological domaini528 – 5358CytoplasmicSequence analysis
Transmembranei536 – 55621HelicalSequence analysisAdd
BLAST
Topological domaini557 – 63276ExtracellularSequence analysisAdd
BLAST
Transmembranei633 – 65321HelicalSequence analysisAdd
BLAST
Topological domaini654 – 6574CytoplasmicSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Mitochondrion

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2073705.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657ATP-binding cassette sub-family G member 2PRO_0000093388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi592 ↔ 610By similarity
Glycosylationi596 – 5961N-linked (GlcNAc...)Sequence analysis
Glycosylationi600 – 6001N-linked (GlcNAc...)Sequence analysis
Disulfide bondi603 – 603InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ7TMS5.
MaxQBiQ7TMS5.
PaxDbiQ7TMS5.
PRIDEiQ7TMS5.

PTM databases

iPTMnetiQ7TMS5.
PhosphoSiteiQ7TMS5.
SwissPalmiQ7TMS5.

Expressioni

Tissue specificityi

Highly expressed in kidney. Lower expression in liver, colon, heart, spleen, and placenta.1 Publication

Gene expression databases

BgeeiQ7TMS5.

Interactioni

Subunit structurei

Monomer under reducing conditions, the minimal functional unit is a homodimer; disulfide-linked, but the major oligomeric form in plasma membranes is a homotetramer with possibility of higher order oligomerization up to homododecamers.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ7TMS5. 2 interactions.
MINTiMINT-2515312.
STRINGi10090.ENSMUSP00000031822.

Structurei

3D structure databases

ProteinModelPortaliQ7TMS5.
SMRiQ7TMS5. Positions 37-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini48 – 285238ABC transporterPROSITE-ProRule annotationAdd
BLAST
Domaini389 – 653265ABC transmembrane type-2Add
BLAST

Domaini

The extracellular loop 3 (ECL3) is involved in binding porphyrins and transfer them to other carriers, probably albumin.By similarity

Sequence similaritiesi

Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0061. Eukaryota.
COG0842. LUCA.
COG1131. LUCA.
HOGENOMiHOG000046080.
HOVERGENiHBG050441.
InParanoidiQ7TMS5.
KOiK05681.
OrthoDBiEOG7HXCR2.
PhylomeDBiQ7TMS5.
TreeFamiTF105211.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR030256. ABCG2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR19241:SF209. PTHR19241:SF209. 1 hit.
PfamiPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TMS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSNDHVLV PMSQRNNNGL PRTNSRAVRT LAEGDVLSFH HITYRVKVKS
60 70 80 90 100
GFLVRKTVEK EILSDINGIM KPGLNAILGP TGGGKSSLLD VLAARKDPKG
110 120 130 140 150
LSGDVLINGA PQPAHFKCCS GYVVQDDVVM GTLTVRENLQ FSAALRLPTT
160 170 180 190 200
MKNHEKNERI NTIIKELGLE KVADSKVGTQ FIRGISGGER KRTSIGMELI
210 220 230 240 250
TDPSILFLDE PTTGLDSSTA NAVLLLLKRM SKQGRTIIFS IHQPRYSIFK
260 270 280 290 300
LFDSLTLLAS GKLVFHGPAQ KALEYFASAG YHCEPYNNPA DFFLDVINGD
310 320 330 340 350
SSAVMLNREE QDNEANKTEE PSKGEKPVIE NLSEFYINSA IYGETKAELD
360 370 380 390 400
QLPGAQEKKG TSAFKEPVYV TSFCHQLRWI ARRSFKNLLG NPQASVAQLI
410 420 430 440 450
VTVILGLIIG AIYFDLKYDA AGMQNRAGVL FFLTTNQCFS SVSAVELFVV
460 470 480 490 500
EKKLFIHEYI SGYYRVSSYF FGKVMSDLLP MRFLPSVIFT CVLYFMLGLK
510 520 530 540 550
KTVDAFFIMM FTLIMVAYTA SSMALAIATG QSVVSVATLL MTIAFVFMML
560 570 580 590 600
FSGLLVNLRT IGPWLSWLQY FSIPRYGFTA LQYNEFLGQE FCPGFNVTDN
610 620 630 640 650
STCVNSYAIC TGNEYLINQG IELSPWGLWK NHVALACMII IFLTIAYLKL

LFLKKYS
Length:657
Mass (Da):72,978
Last modified:October 1, 2003 - v1
Checksum:iDCD70C5D9FA2BA5F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231T → M in AAD54216 (PubMed:10485464).Curated
Sequence conflicti492 – 4921V → I in AAD54216 (PubMed:10485464).Curated
Sequence conflicti512 – 5165TLIMV → GLGAE in AAD09189 (PubMed:9850061).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF140218 mRNA. Translation: AAD54216.1.
BC053730 mRNA. Translation: AAH53730.1.
AF103875 mRNA. Translation: AAD09189.1.
CCDSiCCDS20195.1.
RefSeqiNP_036050.1. NM_011920.3.
XP_006506211.1. XM_006506148.2.
XP_006506212.1. XM_006506149.2.
XP_006506213.1. XM_006506150.2.
XP_006506214.1. XM_006506151.2.
XP_011239664.1. XM_011241362.1.
UniGeneiMm.333096.

Genome annotation databases

GeneIDi26357.
KEGGimmu:26357.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF140218 mRNA. Translation: AAD54216.1.
BC053730 mRNA. Translation: AAH53730.1.
AF103875 mRNA. Translation: AAD09189.1.
CCDSiCCDS20195.1.
RefSeqiNP_036050.1. NM_011920.3.
XP_006506211.1. XM_006506148.2.
XP_006506212.1. XM_006506149.2.
XP_006506213.1. XM_006506150.2.
XP_006506214.1. XM_006506151.2.
XP_011239664.1. XM_011241362.1.
UniGeneiMm.333096.

3D structure databases

ProteinModelPortaliQ7TMS5.
SMRiQ7TMS5. Positions 37-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7TMS5. 2 interactions.
MINTiMINT-2515312.
STRINGi10090.ENSMUSP00000031822.

Chemistry

ChEMBLiCHEMBL2073705.

PTM databases

iPTMnetiQ7TMS5.
PhosphoSiteiQ7TMS5.
SwissPalmiQ7TMS5.

Proteomic databases

EPDiQ7TMS5.
MaxQBiQ7TMS5.
PaxDbiQ7TMS5.
PRIDEiQ7TMS5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi26357.
KEGGimmu:26357.

Organism-specific databases

CTDi9429.
MGIiMGI:1347061. Abcg2.

Phylogenomic databases

eggNOGiKOG0061. Eukaryota.
COG0842. LUCA.
COG1131. LUCA.
HOGENOMiHOG000046080.
HOVERGENiHBG050441.
InParanoidiQ7TMS5.
KOiK05681.
OrthoDBiEOG7HXCR2.
PhylomeDBiQ7TMS5.
TreeFamiTF105211.

Enzyme and pathway databases

ReactomeiR-MMU-2161517. Abacavir transmembrane transport.

Miscellaneous databases

PROiQ7TMS5.
SOURCEiSearch...

Gene expression databases

BgeeiQ7TMS5.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR030256. ABCG2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR19241:SF209. PTHR19241:SF209. 1 hit.
PfamiPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse Bcrp1/Mxr/Abcp gene: amplification and overexpression in cell lines selected for resistance to topotecan, mitoxantrone, or doxorubicin."
    Allen J.D., Brinkhuis R.F., Wijnholds J., Schinkel A.H.
    Cancer Res. 59:4237-4241(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: FVB/NJ.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6NCr.
    Tissue: Hematopoietic stem cell.
  3. "A human placenta-specific ATP-binding cassette gene (ABCP) on chromosome 4q22 that is involved in multidrug resistance."
    Allikmets R., Schriml L.M., Hutchinson A., Romano-Spica V., Dean M.
    Cancer Res. 58:5337-5339(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 511-657.
    Strain: C57BL/6J.
    Tissue: Placenta.
  4. "Role of breast cancer resistance protein in the bioavailability and fetal penetration of topotecan."
    Jonker J.W., Smit J.W., Brinkhuis R.F., Maliepaard M., Beijnen J.H., Schellens J.H., Schinkel A.H.
    J. Natl. Cancer Inst. 92:1651-1656(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype."
    Zhou S., Schuetz J.D., Bunting K.D., Colapietro A.M., Sampath J., Morris J.J., Lagutina I., Grosveld G.C., Osawa M., Nakauchi H., Sorrentino B.P.
    Nat. Med. 7:1028-1034(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Liver and Spleen.

Entry informationi

Entry nameiABCG2_MOUSE
AccessioniPrimary (citable) accession number: Q7TMS5
Secondary accession number(s): Q9R004, Q9Z1T0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 1, 2003
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.