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Protein

Lysosomal Pro-X carboxypeptidase

Gene

Prcp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH (By similarity).By similarity

Catalytic activityi

Cleavage of a -Pro-|-Xaa bond to release a C-terminal amino acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771Charge relay systemSequence analysis
Active sitei428 – 4281Charge relay systemSequence analysis
Active sitei453 – 4531Charge relay systemSequence analysis

GO - Molecular functioni

GO - Biological processi

  • angiogenesis involved in wound healing Source: MGI
  • energy homeostasis Source: MGI
  • glucose homeostasis Source: MGI
  • negative regulation of systemic arterial blood pressure Source: MGI
  • plasma kallikrein-kinin cascade Source: MGI
  • proteolysis Source: GO_Central
  • regulation of blood vessel endothelial cell migration Source: MGI
  • regulation of reactive oxygen species metabolic process Source: MGI
  • regulation of thyroid hormone mediated signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.16.2. 3474.
ReactomeiR-MMU-140837. Intrinsic Pathway of Fibrin Clot Formation.

Protein family/group databases

ESTHERimouse-pcp. Prolylcarboxypeptidase.
MEROPSiS28.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal Pro-X carboxypeptidase (EC:3.4.16.2)
Alternative name(s):
Proline carboxypeptidase
Prolylcarboxypeptidase
Short name:
PRCP
Gene namesi
Name:Prcp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1919711. Prcp.

Subcellular locationi

GO - Cellular componenti

  • basal part of cell Source: MGI
  • extracellular exosome Source: MGI
  • lysosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1255149.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence analysisAdd
BLAST
Propeptidei18 – 4326By similarityPRO_0000027310Add
BLAST
Chaini44 – 491448Lysosomal Pro-X carboxypeptidasePRO_0000027311Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence analysis
Disulfide bondi213 ↔ 370By similarity
Disulfide bondi231 ↔ 308By similarity
Disulfide bondi262 ↔ 341By similarity
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence analysis
Glycosylationi334 – 3341N-linked (GlcNAc...)Sequence analysis
Glycosylationi343 – 3431N-linked (GlcNAc...)Sequence analysis
Disulfide bondi362 ↔ 392By similarity
Glycosylationi413 – 4131N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiQ7TMR0.
MaxQBiQ7TMR0.
PaxDbiQ7TMR0.
PeptideAtlasiQ7TMR0.
PRIDEiQ7TMR0.

PTM databases

PhosphoSiteiQ7TMR0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000061119.
CleanExiMM_PRCP.
GenevisibleiQ7TMR0. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ7TMR0. 1 interaction.
MINTiMINT-4118459.
STRINGi10090.ENSMUSP00000075429.

Chemistry

BindingDBiQ7TMR0.

Structurei

3D structure databases

ProteinModelPortaliQ7TMR0.
SMRiQ7TMR0. Positions 44-489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni192 – 332141SKS domainBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S28 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2183. Eukaryota.
ENOG410Y38A. LUCA.
GeneTreeiENSGT00530000063027.
HOGENOMiHOG000238311.
HOVERGENiHBG005526.
InParanoidiQ7TMR0.
KOiK01285.
OMAiLEVRHMK.
OrthoDBiEOG091G056F.
PhylomeDBiQ7TMR0.
TreeFamiTF314414.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR008758. Peptidase_S28.
[Graphical view]
PfamiPF05577. Peptidase_S28. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TMR0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCRALLLLS FLLLGAATTI PPRLKTLGSP HLSASPTPDP AVARKYSVLY
60 70 80 90 100
FEQKVDHFGF ADMRTFKQRY LVADKHWQRN GGSILFYTGN EGDIVWFCNN
110 120 130 140 150
TGFMWDVAEE LKAMLVFAEH RYYGESLPFG QDSFKDSQHL NFLTSEQALA
160 170 180 190 200
DFAELIRHLE KTIPGAQGQP VIAIGGSYGG MLAAWFRMKY PHIVVGALAA
210 220 230 240 250
SAPIWQLDGM VPCGEFMKIV TNDFRKSGPY CSESIRKSWN VIDKLSGSGS
260 270 280 290 300
GLQSLTNILH LCSPLTSEKI PTLKGWIAET WVNLAMVNYP YACNFLQPLP
310 320 330 340 350
AWPIKEVCQY LKNPNVSDTV LLQNIFQALS VYYNYSGQAA CLNISQTTTS
360 370 380 390 400
SLGSMGWSFQ ACTEMVMPFC TNGIDDMFEP FLWDLEKYSN DCFNQWGVKP
410 420 430 440 450
RPHWMTTMYG GKNISSHSNI IFSNGELDPW SGGGVTRDIT DTLVAINIHD
460 470 480 490
GAHHLDLRAH NAFDPSSVLL SRLLEVKHMK KWILDFYSNI Q
Length:491
Mass (Da):55,027
Last modified:July 5, 2005 - v2
Checksum:iEC97198BF9E097EF
GO
Isoform 2 (identifier: Q7TMR0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     102-491: GFMWDVAEEL...WILDFYSNIQ → VCIH

Note: No experimental confirmation available.
Show »
Length:105
Mass (Da):11,803
Checksum:iCB8271212C1D2165
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti426 – 4261E → K in BAE38679 (PubMed:16141072).Curated
Sequence conflicti426 – 4261E → K in AAH55022 (PubMed:15489334).Curated
Sequence conflicti449 – 4491H → P in BAE38679 (PubMed:16141072).Curated
Sequence conflicti449 – 4491H → P in AAH55022 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei102 – 491390GFMWD…YSNIQ → VCIH in isoform 2. 1 PublicationVSP_039456Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011816 mRNA. Translation: BAB27858.1.
AK051677 mRNA. Translation: BAC34716.1.
AK054462 mRNA. Translation: BAE20697.1.
AK166282 mRNA. Translation: BAE38679.1.
BC026424 mRNA. Translation: AAH26424.1.
BC055022 mRNA. Translation: AAH55022.1.
CCDSiCCDS21452.1. [Q7TMR0-1]
RefSeqiNP_082519.1. NM_028243.3. [Q7TMR0-1]
UniGeneiMm.389969.

Genome annotation databases

EnsembliENSMUST00000076052; ENSMUSP00000075429; ENSMUSG00000061119. [Q7TMR0-1]
GeneIDi72461.
KEGGimmu:72461.
UCSCiuc009iij.2. mouse. [Q7TMR0-2]
uc009iik.2. mouse. [Q7TMR0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011816 mRNA. Translation: BAB27858.1.
AK051677 mRNA. Translation: BAC34716.1.
AK054462 mRNA. Translation: BAE20697.1.
AK166282 mRNA. Translation: BAE38679.1.
BC026424 mRNA. Translation: AAH26424.1.
BC055022 mRNA. Translation: AAH55022.1.
CCDSiCCDS21452.1. [Q7TMR0-1]
RefSeqiNP_082519.1. NM_028243.3. [Q7TMR0-1]
UniGeneiMm.389969.

3D structure databases

ProteinModelPortaliQ7TMR0.
SMRiQ7TMR0. Positions 44-489.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7TMR0. 1 interaction.
MINTiMINT-4118459.
STRINGi10090.ENSMUSP00000075429.

Chemistry

BindingDBiQ7TMR0.
ChEMBLiCHEMBL1255149.

Protein family/group databases

ESTHERimouse-pcp. Prolylcarboxypeptidase.
MEROPSiS28.001.

PTM databases

PhosphoSiteiQ7TMR0.

Proteomic databases

EPDiQ7TMR0.
MaxQBiQ7TMR0.
PaxDbiQ7TMR0.
PeptideAtlasiQ7TMR0.
PRIDEiQ7TMR0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076052; ENSMUSP00000075429; ENSMUSG00000061119. [Q7TMR0-1]
GeneIDi72461.
KEGGimmu:72461.
UCSCiuc009iij.2. mouse. [Q7TMR0-2]
uc009iik.2. mouse. [Q7TMR0-1]

Organism-specific databases

CTDi5547.
MGIiMGI:1919711. Prcp.

Phylogenomic databases

eggNOGiKOG2183. Eukaryota.
ENOG410Y38A. LUCA.
GeneTreeiENSGT00530000063027.
HOGENOMiHOG000238311.
HOVERGENiHBG005526.
InParanoidiQ7TMR0.
KOiK01285.
OMAiLEVRHMK.
OrthoDBiEOG091G056F.
PhylomeDBiQ7TMR0.
TreeFamiTF314414.

Enzyme and pathway databases

BRENDAi3.4.16.2. 3474.
ReactomeiR-MMU-140837. Intrinsic Pathway of Fibrin Clot Formation.

Miscellaneous databases

PROiQ7TMR0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000061119.
CleanExiMM_PRCP.
GenevisibleiQ7TMR0. MM.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR008758. Peptidase_S28.
[Graphical view]
PfamiPF05577. Peptidase_S28. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPCP_MOUSE
AccessioniPrimary (citable) accession number: Q7TMR0
Secondary accession number(s): Q3TLW3
, Q3V302, Q8BKK3, Q8R0T8, Q9D049
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: September 7, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.