Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Heterogeneous nuclear ribonucleoprotein Q

Gene

Syncrip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Heterogeneous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1 and isoform 2 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences (By similarity). Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself (By similarity). May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (By similarity). Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 2 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein Q
Short name:
hnRNP Q
Alternative name(s):
Glycine- and tyrosine-rich RNA-binding protein
Short name:
GRY-RBP
NS1-associated protein 1
Synaptotagmin-binding, cytoplasmic RNA-interacting protein
pp68
Gene namesi
Name:Syncrip
Synonyms:Hnrpq, Nsap1, Nsap1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1891690. Syncrip.

Subcellular locationi

  • Nucleus 2 Publications
  • Nucleusnucleoplasm By similarity
  • Microsome 1 Publication
  • Cytoplasm 1 Publication

  • Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs (By similarity). Isoforms 1 and 2 are expressed predominantly in the nucleoplasm. According to PubMed:10734137, isoform 2 is predominantly found in cytoplasm. The tyrosine phosphorylated form bound to RNA is found in microsomes.By similarity1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Microsome, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 623622Heterogeneous nuclear ribonucleoprotein QPRO_0000081868Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Cross-linki123 – 123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei159 – 1591PhosphoserineBy similarity
Modified residuei221 – 2211N6-acetyllysineBy similarity
Modified residuei363 – 3631N6-acetyllysineBy similarity
Modified residuei373 – 3731PhosphotyrosineBy similarity
Modified residuei444 – 4441Asymmetric dimethylarginine; by PRMT1; alternateBy similarity
Modified residuei444 – 4441Omega-N-methylarginine; by PRMT1; alternateBy similarity
Modified residuei496 – 4961Omega-N-methylarginine; by PRMT1By similarity
Modified residuei510 – 5101Asymmetric dimethylarginine; by PRMT1By similarity
Modified residuei518 – 5181Asymmetric dimethylarginine; by PRMT1; alternateBy similarity
Modified residuei518 – 5181Omega-N-methylarginine; by PRMT1; alternateBy similarity
Modified residuei526 – 5261Asymmetric dimethylarginine; alternateBy similarity
Modified residuei526 – 5261Omega-N-methylarginine; alternateBy similarity
Modified residuei536 – 5361Asymmetric dimethylarginine; by PRMT1; alternateBy similarity
Modified residuei536 – 5361Omega-N-methylarginine; by PRMT1; alternateBy similarity
Modified residuei539 – 5391Asymmetric dimethylarginine; by PRMT1; alternateBy similarity
Modified residuei539 – 5391Omega-N-methylarginine; by PRMT1; alternateBy similarity
Modified residuei587 – 5871PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on tyrosine. The membrane-bound form found in microsomes is phosphorylated in vitro by insulin receptor tyrosine kinase (INSR). Phosphorylation is inhibited upon binding to RNA, whereas the cytoplasmic form is poorly phosphorylated.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ7TMK9.
MaxQBiQ7TMK9.
PaxDbiQ7TMK9.
PeptideAtlasiQ7TMK9.
PRIDEiQ7TMK9.

2D gel databases

REPRODUCTION-2DPAGEIPI00406117.

PTM databases

iPTMnetiQ7TMK9.
PhosphoSiteiQ7TMK9.
SwissPalmiQ7TMK9.

Expressioni

Tissue specificityi

Ubiquitous. Detected in heart, brain, spleen, lung, liver, skeletal muscle, adipocytes, kidney and testis.3 Publications

Developmental stagei

Expressed in spinal cord at 14 dpc and onwards.1 Publication

Gene expression databases

BgeeiENSMUSG00000032423.
ExpressionAtlasiQ7TMK9. baseline and differential.
GenevisibleiQ7TMK9. MM.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with GTPBP1 (By similarity). Isoform 1 is a component of the APOB mRNA editosome complex. Isoform 1 interacts with APOBEC1 and A1CF. Part of a complex associated with the FOS mCRD domain and consisting of PABPC1, PAIP1, CSDE1/UNR, HNRPD and SYNCRIP. Isoform 2 interacts with HNRPR. Interacts with POLR2A hyperphosphorylated C-terminal domain. Interacts with minute virus of mice (MVM) NS1 protein (By similarity). Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1. Isoform 1 and isoform 2 interact with SMN. Isoform 2 interacts through its C-terminal domain with SYT7, SYT8 and SYT9. The non-phosphorylated and phosphorylated forms are colocalized with PAIP1 in polysomes.By similarity3 Publications

Protein-protein interaction databases

BioGridi207955. 6 interactions.
IntActiQ7TMK9. 3 interactions.
MINTiMINT-4097748.
STRINGi10090.ENSMUSP00000133649.

Structurei

3D structure databases

ProteinModelPortaliQ7TMK9.
SMRiQ7TMK9. Positions 24-423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini162 – 24180RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini243 – 32583RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini338 – 40871RRM 3PROSITE-ProRule annotationAdd
BLAST
Repeati448 – 45031-1
Repeati451 – 45331-2
Repeati460 – 46452-1
Repeati469 – 47242-2
Repeati478 – 48031-3
Repeati485 – 48842-3
Repeati498 – 50031-4
Repeati526 – 52831-5
Repeati539 – 54131-6
Repeati554 – 55631-7
Repeati557 – 55931-8

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni400 – 561162Interaction with APOBEC1By similarityAdd
BLAST
Regioni448 – 5591128 X 3 AA repeats of R-G-GAdd
BLAST
Regioni460 – 488293 X 4 AA repeats of Y-Y-G-YAdd
BLAST
Regioni518 – 54932Interaction with SMNBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi564 – 57815Bipartite nuclear localization signalSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi431 – 4344Poly-Tyr

Domaini

The domain containing eight Arg-Gly-Gly repeats (RGG/RXR-box) may be involved in RNA-binding and protein-protein interactions. It is methylated by PRMT1, and essential for nuclear localization (By similarity).By similarity

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0117. Eukaryota.
ENOG410XTJ5. LUCA.
GeneTreeiENSGT00550000074366.
HOVERGENiHBG051917.
InParanoidiQ7TMK9.
KOiK13160.
OMAiMATDHVN.
OrthoDBiEOG091G0EUB.
PhylomeDBiQ7TMK9.
TreeFamiTF314932.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR006535. HnRNP_R/Q_splicing_fac.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
TIGRFAMsiTIGR01648. hnRNP-R-Q. 1 hit.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7TMK9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATEHVNGNG TEEPMDTTSA VIHSENFQTL LDAGLPQKVA EKLDEIYVAG
60 70 80 90 100
LVAHSDLDER AIEALKEFNE DGALAVLQQF KDSDLSHVQN KSAFLCGVMK
110 120 130 140 150
TYRQREKQGT KVADSSKGPD EAKIKALLER TGYTLDVTTG QRKYGGPPPD
160 170 180 190 200
SVYSGQQPSV GTEIFVGKIP RDLFEDELVP LFEKAGPIWD LRLMMDPLTG
210 220 230 240 250
LNRGYAFVTF CTKEAAQEAV KLYNNHEIRS GKHIGVCISV ANNRLFVGSI
260 270 280 290 300
PKSKTKEQIL EEFSKVTEGL TDVILYHQPD DKKKNRGFCF LEYEDHKTAA
310 320 330 340 350
QARRRLMSGK VKVWGNVGTV EWADPIEDPD PEVMAKVKVL FVRNLANTVT
360 370 380 390 400
EEILEKSFSQ FGKLERVKKL KDYAFIHFDE RDGAVKAMEE MNGKDLEGEN
410 420 430 440 450
IEIVFAKPPD QKRKERKAQR QAAKNQMYDD YYYYGPPHMP PPTRGRGRGG
460 470 480 490 500
RGGYGYPPDY YGYEDYYDYY GYDYHNYRGG YEDPYYGYED FQVGARGRGG
510 520 530 540 550
RGARGAAPSR GRGAAPPRGR AGYSQRGGPG SARGVRGARG GAQQQRGRGV
560 570 580 590 600
RGARGGRGGN VGGKRKADGY NQPDTKRRQT NNQNWGSQPI AQQPLQGGDH
610 620
SGNYGYKSEN QEFYQDTFGQ QWK
Length:623
Mass (Da):69,633
Last modified:March 1, 2004 - v2
Checksum:i8139FF9E2C8FB304
GO
Isoform 2 (identifier: Q7TMK9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     550-562: VRGARGGRGGNVG → QGKGVEAGPDLLQ
     563-623: Missing.

Note: May be due to a competing donor splice site and to an exon inclusion.Curated
Show »
Length:562
Mass (Da):62,672
Checksum:i810BCFA286105377
GO

Sequence cautioni

The sequence AAH55863 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH58807 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti143 – 1431K → E in AAH58807 (PubMed:15489334).Curated
Sequence conflicti194 – 1941M → T in AAH50079 (PubMed:15489334).Curated
Sequence conflicti201 – 2011L → LTGL in AAC62511 (Ref. 1) Curated
Sequence conflicti220 – 2201V → A in AAC62511 (Ref. 1) Curated
Sequence conflicti291 – 2911L → Q in AAC62511 (Ref. 1) Curated
Sequence conflicti298 – 2981T → A in AAC62511 (Ref. 1) Curated
Sequence conflicti407 – 4071K → N in BAC37152 (PubMed:16141072).Curated
Sequence conflicti411 – 4111Q → K in BAC37152 (PubMed:16141072).Curated
Sequence conflicti421 – 4211Q → H in BAC37152 (PubMed:16141072).Curated
Sequence conflicti528 – 5281G → A in AAC62511 (Ref. 1) Curated
Sequence conflicti543 – 5431Missing in AAC62511 (Ref. 1) Curated
Sequence conflicti582 – 5821N → H in AAC62511 (Ref. 1) Curated
Isoform 2 (identifier: Q7TMK9-2)
Sequence conflicti549 – 5502GQ → G in BAA88342 (PubMed:10734137).Curated
Sequence conflicti549 – 5502GQ → G in AAH41148 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei550 – 56213VRGAR…GGNVG → QGKGVEAGPDLLQ in isoform 2. 4 PublicationsVSP_009585Add
BLAST
Alternative sequencei563 – 62361Missing in isoform 2. 4 PublicationsVSP_009586Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093821 mRNA. Translation: AAC62511.1.
AB035725 mRNA. Translation: BAA88342.1.
AK034845 mRNA. Translation: BAC28852.1.
AK077588 mRNA. Translation: BAC36880.1.
AK078158 mRNA. Translation: BAC37152.1.
BC004001 mRNA. Translation: AAH04001.1. Sequence problems.
BC041148 mRNA. Translation: AAH41148.2.
BC050079 mRNA. Translation: AAH50079.2.
BC055863 mRNA. Translation: AAH55863.1. Different initiation.
BC058807 mRNA. Translation: AAH58807.1. Different initiation.
BC080309 mRNA. Translation: AAH80309.1.
BC108363 mRNA. Translation: AAI08364.2.
AF408434 mRNA. Translation: AAL11726.1.
CCDSiCCDS23388.1. [Q7TMK9-2]
CCDS57686.1. [Q7TMK9-1]
RefSeqiNP_001271257.1. NM_001284328.1.
NP_001298042.1. NM_001311113.1.
NP_062640.2. NM_019666.2. [Q7TMK9-1]
NP_062770.1. NM_019796.5. [Q7TMK9-2]
UniGeneiMm.260545.

Genome annotation databases

EnsembliENSMUST00000069221; ENSMUSP00000063744; ENSMUSG00000032423. [Q7TMK9-2]
ENSMUST00000173801; ENSMUSP00000133649; ENSMUSG00000032423. [Q7TMK9-1]
GeneIDi56403.
KEGGimmu:56403.
UCSCiuc009qyo.2. mouse. [Q7TMK9-2]
uc009qyq.2. mouse. [Q7TMK9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093821 mRNA. Translation: AAC62511.1.
AB035725 mRNA. Translation: BAA88342.1.
AK034845 mRNA. Translation: BAC28852.1.
AK077588 mRNA. Translation: BAC36880.1.
AK078158 mRNA. Translation: BAC37152.1.
BC004001 mRNA. Translation: AAH04001.1. Sequence problems.
BC041148 mRNA. Translation: AAH41148.2.
BC050079 mRNA. Translation: AAH50079.2.
BC055863 mRNA. Translation: AAH55863.1. Different initiation.
BC058807 mRNA. Translation: AAH58807.1. Different initiation.
BC080309 mRNA. Translation: AAH80309.1.
BC108363 mRNA. Translation: AAI08364.2.
AF408434 mRNA. Translation: AAL11726.1.
CCDSiCCDS23388.1. [Q7TMK9-2]
CCDS57686.1. [Q7TMK9-1]
RefSeqiNP_001271257.1. NM_001284328.1.
NP_001298042.1. NM_001311113.1.
NP_062640.2. NM_019666.2. [Q7TMK9-1]
NP_062770.1. NM_019796.5. [Q7TMK9-2]
UniGeneiMm.260545.

3D structure databases

ProteinModelPortaliQ7TMK9.
SMRiQ7TMK9. Positions 24-423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207955. 6 interactions.
IntActiQ7TMK9. 3 interactions.
MINTiMINT-4097748.
STRINGi10090.ENSMUSP00000133649.

PTM databases

iPTMnetiQ7TMK9.
PhosphoSiteiQ7TMK9.
SwissPalmiQ7TMK9.

2D gel databases

REPRODUCTION-2DPAGEIPI00406117.

Proteomic databases

EPDiQ7TMK9.
MaxQBiQ7TMK9.
PaxDbiQ7TMK9.
PeptideAtlasiQ7TMK9.
PRIDEiQ7TMK9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069221; ENSMUSP00000063744; ENSMUSG00000032423. [Q7TMK9-2]
ENSMUST00000173801; ENSMUSP00000133649; ENSMUSG00000032423. [Q7TMK9-1]
GeneIDi56403.
KEGGimmu:56403.
UCSCiuc009qyo.2. mouse. [Q7TMK9-2]
uc009qyq.2. mouse. [Q7TMK9-1]

Organism-specific databases

CTDi10492.
MGIiMGI:1891690. Syncrip.

Phylogenomic databases

eggNOGiKOG0117. Eukaryota.
ENOG410XTJ5. LUCA.
GeneTreeiENSGT00550000074366.
HOVERGENiHBG051917.
InParanoidiQ7TMK9.
KOiK13160.
OMAiMATDHVN.
OrthoDBiEOG091G0EUB.
PhylomeDBiQ7TMK9.
TreeFamiTF314932.

Miscellaneous databases

PROiQ7TMK9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032423.
ExpressionAtlasiQ7TMK9. baseline and differential.
GenevisibleiQ7TMK9. MM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR006535. HnRNP_R/Q_splicing_fac.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
TIGRFAMsiTIGR01648. hnRNP-R-Q. 1 hit.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHNRPQ_MOUSE
AccessioniPrimary (citable) accession number: Q7TMK9
Secondary accession number(s): O88991
, Q2YDV8, Q68ED6, Q80YV6, Q8BGP1, Q8C5K6, Q8CGC2, Q91ZR0, Q99KU9, Q9QYF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: September 7, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.