Reviewed, UniProtKB/Swiss-Prot Q7TMF5 (SPA12_MOUSE)

Names and origin

Protein names

Recommended name:
    Serpin A12
Alternative name(s):
    Visceral adipose tissue-derived serine protease inhibitor
      Short name=Vaspin
    Visceral adipose-specific serpin

Gene names

Name:

Serpina12

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May modulates insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues. Ref.1
Subcellular location	Secreted Potential.
Tissue specificity	Expressed in visceral adipose tissues. Ref.1
Sequence similarities	Belongs to the serpin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20
	Chain	21 – 413	393	Serpin A12		PRO_0000041977
Amino acid modifications
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	267	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	69	1	Q → R in AAP88383. Ref.1
	Sequence conflict	110	1	W → R in AAP88383. Ref.1
	Sequence conflict	183	1	Q → R in AAP88383. Ref.1
	Sequence conflict	403	1	V → I in AAP88383. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q7TMF5-1 [UniParc]. Last modified September 27, 2005. Version 2. Checksum: D0AE8E1EE24FD60A	FASTA	413	47,634
	10 20 30 40 50 60 MTRMLDLGLF LAGLLTVKGL LQDRDAPDMY DSPVRVQEWR GKKDARQLAR HNMEFGFKLL 70 80 90 100 110 120 QRLASNSPQG NIFLSPLSIS TAFSMLSLGA QNSTLEEIRE GFNFKEMSNW DVHAAFHYLL 130 140 150 160 170 180 HKLNQETEDT KMNLGNALFM DQKLRPQQRF LNLAKNVYDA DMVLTNFQDL ENTQKDINRY 190 200 210 220 230 240 ISQKTHSRIK NMVKSIDPGT VMILTNYIYF RGRWQYEFDP KQTKEEEFFI EKGKTVKVPM 250 260 270 280 290 300 MFQRGLYDMA YDSQLSCTIL EIPYRGNITA TFVLPDNGKL KLLEQGLQAD IFAKWKSLLS 310 320 330 340 350 360 KRVVDVWVPK LRISSTYNMK KVLSRLGISK IFEENGDLTR ISSHRSLKVG EAVHKAELKM 370 380 390 400 410 DEKGMEGAAG SGAQTLPMET PRHMKLDRPF LMMIYENFMP SMVFLARIYD PSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Visceral adipose tissue-derived serine protease inhibitor: a unique insulin-sensitizing adipocytokine in obesity." Hida K., Wada J., Eguchi J., Zhang H., Baba M., Seida A., Hashimoto I., Okada T., Yasuhara A., Nakatsuka A., Shikata K., Hourai S., Futami J., Watanabe E., Matsuki Y., Hiramatsu R., Akagi S., Makino H., Kanwar Y.S. Proc. Natl. Acad. Sci. U.S.A. 102:10610-10615(2005) [PubMed: 16030142] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PROTEIN SEQUENCE OF N-TERMINUS, TISSUE SPECIFICITY. Strain: Swiss Webster.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Head and Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY326419 mRNA. Translation: AAP88383.1. AK014346 mRNA. Translation: BAB29287.1. AK014589 mRNA. Translation: BAB29447.1.
IPI	IPI00131951.
RefSeq	NP_080811.1.
UniGene	Mm.20286
3D structure databases
HSSP	HSSP built from PDB template 1ATH based on UniProtKB P01008.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7TMF5.
Protein family/group databases
MEROPS	I04.965.
Proteomic databases
PRIDE	Q7TMF5.
Genome annotation databases
Ensembl	ENSMUST00000043915; ENSMUSP00000045572; ENSMUSG00000041567; Mus musculus. [Genome view]
GeneID	68054.
KEGG	mmu:68054.
UCSC	uc007owp.1. mouse.
Organism-specific databases
CTD	68054.
MGI	MGI:1915304. Serpina12.
Phylogenomic databases
HOGENOM	Q7TMF5.
HOVERGEN	Q7TMF5.
OMA	AQTLPME.
Gene expression databases
ArrayExpress	Q7TMF5.
Bgee	Q7TMF5.
Genevestigator	Q7TMF5.
GermOnline	ENSMUSG00000041567. Mus musculus.
Family and domain databases
InterPro	IPR000295. Prot_inh_Lserp2. IPR000215. Protease_inhib_I4_serpin. [Graphical view]
PANTHER	PTHR11461. Prot_inh_serpin. 1 hit.
Pfam	PF00079. Serpin. 1 hit. [Graphical view]
PRINTS	PR00780. LEUSERPINII.
SMART	SM00093. SERPIN. 1 hit. [Graphical view]
PROSITE	PS00284. SERPIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	326328.
SOURCE	Search...

Entry information

Entry name

SPA12_MOUSE

Accession

Primary (citable) accession number: Q7TMF5
Secondary accession number(s): Q9CQ32

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	September 27, 2005
Last modified:	November 3, 2009
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)