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Q7TMB3

- PPR3C_MOUSE

UniProt

Q7TMB3 - PPR3C_MOUSE

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Protein

Protein phosphatase 1 regulatory subunit 3C

Gene
Ppp1r3c, Ppp1r5
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a glycogen-targeting subunit for PP1 and regulates its activity. Activates glycogen synthase, reduces glycogen phosphorylase activity and limits glycogen breakdown. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in a variety of cell types.10 Publications

GO - Molecular functioni

  1. protein phosphatase binding Source: MGI

GO - Biological processi

  1. glycogen biosynthetic process Source: MGI
  2. glycogen metabolic process Source: MGI
  3. protein targeting Source: MGI
Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Enzyme and pathway databases

ReactomeiREACT_188939. Glycogen synthesis.
REACT_203841. Myoclonic epilepsy of Lafora.

Protein family/group databases

CAZyiCBM21. Carbohydrate-Binding Module Family 21.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 3C
Alternative name(s):
Protein phosphatase 1 regulatory subunit 5
Short name:
PP1 subunit R5
Protein targeting to glycogen
Short name:
PTG
Gene namesi
Name:Ppp1r3c
Synonyms:Ppp1r5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1858229. Ppp1r3c.

Pathology & Biotechi

Disruption phenotypei

Mice display embryonic lethality when homozygous. Heterozygotes display decreased protein levels, decreased glycogen accumulation and glycogen synthase activity, reduced insulin-stimulated glycogen synthesis and progressive age-dependent glucose intolerance. When Ppp1r3c is silenced in adults, they display decreased PP1 activity and glycogen accumulation, increased phosphorylation of glycogen phosphorylase, increased GLUT1 levels, increased glucose uptake and increased glycogen degradation.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi85 – 851V → A: Abolishes binding to PP1 but has no effect on binding to glycogen synthase, glycogen phosphorylase or glycogen; when associated with A-87. 2 Publications
Mutagenesisi87 – 871F → A: Abolishes binding to PP1 but has no effect on binding to glycogen synthase, glycogen phosphorylase or glycogen; when associated with A-85. 2 Publications
Mutagenesisi247 – 2471D → A: Abolishes binding to glycogen synthase and glycogen phosphorylase but has no effect on binding to PP1 or glycogen; when associated with A-250. 2 Publications
Mutagenesisi250 – 2501E → A: Abolishes binding to glycogen synthase and glycogen phosphorylase but has no effect on binding to PP1 or glycogen; when associated with A-247. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Protein phosphatase 1 regulatory subunit 3CPRO_0000285928Add
BLAST

Post-translational modificationi

Ubiquitinated by NHLRC1/malin in a EPM2A/laforin-dependent manner By similarity.

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ7TMB3.
PRIDEiQ7TMB3.

Expressioni

Gene expression databases

BgeeiQ7TMB3.
GenevestigatoriQ7TMB3.

Interactioni

Subunit structurei

Interacts with PPP1CC catalytic subunit of PP1 and associates with glycogen. Forms complexes with glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity. Also interacts with EPM2A/laforin.2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084578.

Structurei

3D structure databases

ProteinModelPortaliQ7TMB3.
SMRiQ7TMB3. Positions 134-262.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini149 – 257109CBM21Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni141 – 263123Interaction with EPM2A By similarity1 PublicationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi84 – 874PP1-binding motif

Domaini

The N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to glycogen phosphorylase, glycogen synthase and phosphorylase kinase.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG288354.
GeneTreeiENSGT00530000062978.
HOGENOMiHOG000026799.
HOVERGENiHBG052744.
InParanoidiQ7TMB3.
KOiK07189.
OMAiIVHVQWK.
OrthoDBiEOG7H4DTW.
PhylomeDBiQ7TMB3.
TreeFamiTF105537.

Family and domain databases

InterProiIPR005036. CBM_21.
IPR017434. Pase-1_Glycogen_target-su_met.
[Graphical view]
PfamiPF03370. CBM_21. 1 hit.
[Graphical view]
PIRSFiPIRSF038207. PP1_GT_animal. 1 hit.
PROSITEiPS51159. CBM21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7TMB3-1 [UniParc]FASTAAdd to Basket

« Hide

MSCTRMIHVL DPRPLTSSVM PVDMAMRICL AHSPPLKSFL GPYNGFQRRN    50
FVNKLKPLKP CLSVKQEAKS QSEWKSPHNQ AKKRVVFADS KGLSLTAIHV 100
FSDLPEEPAW DLQFDLLDLN DISSSLKLHE EKNLVFDFPQ PSTDYLSFRD 150
RFQKNFVCLE NCSLQDRTVT GTVKVKNVSF EKKVQVRITF DTWKTYTDVD 200
CVYMKNVYSS SDSDTFSFAI DLPRVIPTEE KIEFCISYHA NGRIFWDNNE 250
GQNYRIVHVQ WKPDGVQTQV APKDCAFQQG PPKTEIEPTV FGSPRLASGL 300
FPEWQSWGRV ENLTSYR 317
Length:317
Mass (Da):36,460
Last modified:October 1, 2003 - v1
Checksum:i8DC8941B733649D6
GO

Sequence cautioni

The sequence AAB49689.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti165 – 1651Q → E in AAB49689. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC052769 mRNA. Translation: AAH52769.1.
BC054739 mRNA. Translation: AAH54739.1.
U89924 mRNA. Translation: AAB49689.1. Different initiation.
AK078506 mRNA. Translation: BAC37313.1.
CCDSiCCDS37968.1.
RefSeqiNP_058550.1. NM_016854.2.
UniGeneiMm.24724.

Genome annotation databases

EnsembliENSMUST00000087321; ENSMUSP00000084578; ENSMUSG00000067279.
GeneIDi53412.
KEGGimmu:53412.
UCSCiuc008hhs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC052769 mRNA. Translation: AAH52769.1 .
BC054739 mRNA. Translation: AAH54739.1 .
U89924 mRNA. Translation: AAB49689.1 . Different initiation.
AK078506 mRNA. Translation: BAC37313.1 .
CCDSi CCDS37968.1.
RefSeqi NP_058550.1. NM_016854.2.
UniGenei Mm.24724.

3D structure databases

ProteinModelPortali Q7TMB3.
SMRi Q7TMB3. Positions 134-262.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000084578.

Protein family/group databases

CAZyi CBM21. Carbohydrate-Binding Module Family 21.

Proteomic databases

PaxDbi Q7TMB3.
PRIDEi Q7TMB3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000087321 ; ENSMUSP00000084578 ; ENSMUSG00000067279 .
GeneIDi 53412.
KEGGi mmu:53412.
UCSCi uc008hhs.1. mouse.

Organism-specific databases

CTDi 5507.
MGIi MGI:1858229. Ppp1r3c.

Phylogenomic databases

eggNOGi NOG288354.
GeneTreei ENSGT00530000062978.
HOGENOMi HOG000026799.
HOVERGENi HBG052744.
InParanoidi Q7TMB3.
KOi K07189.
OMAi IVHVQWK.
OrthoDBi EOG7H4DTW.
PhylomeDBi Q7TMB3.
TreeFami TF105537.

Enzyme and pathway databases

Reactomei REACT_188939. Glycogen synthesis.
REACT_203841. Myoclonic epilepsy of Lafora.

Miscellaneous databases

NextBioi 310221.
PROi Q7TMB3.
SOURCEi Search...

Gene expression databases

Bgeei Q7TMB3.
Genevestigatori Q7TMB3.

Family and domain databases

InterProi IPR005036. CBM_21.
IPR017434. Pase-1_Glycogen_target-su_met.
[Graphical view ]
Pfami PF03370. CBM_21. 1 hit.
[Graphical view ]
PIRSFi PIRSF038207. PP1_GT_animal. 1 hit.
PROSITEi PS51159. CBM21. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Olfactory epithelium.
  2. "PTG, a protein phosphatase 1-binding protein with a role in glycogen metabolism."
    Printen J.A., Brady M.J., Saltiel A.R.
    Science 275:1475-1478(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-317, FUNCTION, ASSOCIATION WITH GLYCOGEN, INTERACTION WITH PPP1CC.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 126-317.
    Strain: C57BL/6J.
    Tissue: Muellerian duct.
  4. "Role of protein targeting to glycogen (PTG) in the regulation of protein phosphatase-1 activity."
    Brady M.J., Printen J.A., Mastick C.C., Saltiel A.R.
    J. Biol. Chem. 272:20198-20204(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Overexpression of protein targeting to glycogen (PTG) in rat hepatocytes causes profound activation of glycogen synthesis independent of normal hormone- and substrate-mediated regulatory mechanisms."
    Berman H.K., O'Doherty R.M., Anderson P., Newgard C.B.
    J. Biol. Chem. 273:26421-26425(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Distinctive regulatory and metabolic properties of glycogen-targeting subunits of protein phosphatase-1 (PTG, GL, GM/RGl) expressed in hepatocytes."
    Gasa R., Jensen P.B., Berman H.K., Brady M.J., DePaoli-Roach A.A., Newgard C.B.
    J. Biol. Chem. 275:26396-26403(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Identification of binding sites on protein targeting to glycogen for enzymes of glycogen metabolism."
    Fong N.M., Jensen T.C., Shah A.S., Parekh N.N., Saltiel A.R., Brady M.J.
    J. Biol. Chem. 275:35034-35039(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF VAL-85; PHE-87; ASP-247 AND GLU-250.
  8. "Overexpression of protein targeting to glycogen in cultured human muscle cells stimulates glycogen synthesis independent of glycogen and glucose 6-phosphate levels."
    Lerin C., Montell E., Berman H.K., Newgard C.B., Gomez-Foix A.M.
    J. Biol. Chem. 275:39991-39995(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Activation of direct and indirect pathways of glycogen synthesis by hepatic overexpression of protein targeting to glycogen."
    O'Doherty R.M., Jensen P.B., Anderson P., Jones J.G., Berman H.K., Kearney D., Newgard C.B.
    J. Clin. Invest. 105:479-488(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Protein targeting to glycogen overexpression results in the specific enhancement of glycogen storage in 3T3-L1 adipocytes."
    Greenberg C.C., Meredith K.N., Yan L., Brady M.J.
    J. Biol. Chem. 278:30835-30842(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "PTG gene deletion causes impaired glycogen synthesis and developmental insulin resistance."
    Crosson S.M., Khan A., Printen J., Pessin J.E., Saltiel A.R.
    J. Clin. Invest. 111:1423-1432(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  12. "The glycogenic action of protein targeting to glycogen in hepatocytes involves multiple mechanisms including phosphorylase inactivation and glycogen synthase translocation."
    Green A.R., Aiston S., Greenberg C.C., Freeman S., Poucher S.M., Brady M.J., Agius L.
    J. Biol. Chem. 279:46474-46482(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Central role for protein targeting to glycogen in the maintenance of cellular glycogen stores in 3T3-L1 adipocytes."
    Greenberg C.C., Danos A.M., Brady M.J.
    Mol. Cell. Biol. 26:334-342(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPPR3C_MOUSE
AccessioniPrimary (citable) accession number: Q7TMB3
Secondary accession number(s): O08541, Q8BJW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 1, 2003
Last modified: September 3, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi