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Q7TMB3 (PPR3C_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 1 regulatory subunit 3C
Alternative name(s):
Protein phosphatase 1 regulatory subunit 5
Short name=PP1 subunit R5
Protein targeting to glycogen
Short name=PTG
Gene names
Name:Ppp1r3c
Synonyms:Ppp1r5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a glycogen-targeting subunit for PP1 and regulates its activity. Activates glycogen synthase, reduces glycogen phosphorylase activity and limits glycogen breakdown. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in a variety of cell types. Ref.2 Ref.4 Ref.5 Ref.6 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13

Subunit structure

Interacts with PPP1CC catalytic subunit of PP1 and associates with glycogen. Forms complexes with glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity. Also interacts with EPM2A/laforin. Ref.2 Ref.12

Domain

The N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to glycogen phosphorylase, glycogen synthase and phosphorylase kinase. Ref.11

Post-translational modification

Ubiquitinated by NHLRC1/malin in a EPM2A/laforin-dependent manner By similarity.

Disruption phenotype

Mice display embryonic lethality when homozygous. Heterozygotes display decreased protein levels, decreased glycogen accumulation and glycogen synthase activity, reduced insulin-stimulated glycogen synthesis and progressive age-dependent glucose intolerance. When Ppp1r3c is silenced in adults, they display decreased PP1 activity and glycogen accumulation, increased phosphorylation of glycogen phosphorylase, increased GLUT1 levels, increased glucose uptake and increased glycogen degradation. Ref.11 Ref.13

Sequence similarities

Contains 1 CBM21 (carbohydrate binding type-21) domain.

Sequence caution

The sequence AAB49689.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317Protein phosphatase 1 regulatory subunit 3C
PRO_0000285928

Regions

Domain149 – 257109CBM21
Region141 – 263123Interaction with EPM2A By similarity Ref.12
Motif84 – 874PP1-binding motif

Experimental info

Mutagenesis851V → A: Abolishes binding to PP1 but has no effect on binding to glycogen synthase, glycogen phosphorylase or glycogen; when associated with A-87. Ref.7 Ref.11
Mutagenesis871F → A: Abolishes binding to PP1 but has no effect on binding to glycogen synthase, glycogen phosphorylase or glycogen; when associated with A-85. Ref.7 Ref.11
Mutagenesis2471D → A: Abolishes binding to glycogen synthase and glycogen phosphorylase but has no effect on binding to PP1 or glycogen; when associated with A-250. Ref.7 Ref.11
Mutagenesis2501E → A: Abolishes binding to glycogen synthase and glycogen phosphorylase but has no effect on binding to PP1 or glycogen; when associated with A-247. Ref.7 Ref.11
Sequence conflict1651Q → E in AAB49689. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q7TMB3 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 8DC8941B733649D6

FASTA31736,460
        10         20         30         40         50         60 
MSCTRMIHVL DPRPLTSSVM PVDMAMRICL AHSPPLKSFL GPYNGFQRRN FVNKLKPLKP 

        70         80         90        100        110        120 
CLSVKQEAKS QSEWKSPHNQ AKKRVVFADS KGLSLTAIHV FSDLPEEPAW DLQFDLLDLN 

       130        140        150        160        170        180 
DISSSLKLHE EKNLVFDFPQ PSTDYLSFRD RFQKNFVCLE NCSLQDRTVT GTVKVKNVSF 

       190        200        210        220        230        240 
EKKVQVRITF DTWKTYTDVD CVYMKNVYSS SDSDTFSFAI DLPRVIPTEE KIEFCISYHA 

       250        260        270        280        290        300 
NGRIFWDNNE GQNYRIVHVQ WKPDGVQTQV APKDCAFQQG PPKTEIEPTV FGSPRLASGL 

       310 
FPEWQSWGRV ENLTSYR 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain and Olfactory epithelium.
[2]"PTG, a protein phosphatase 1-binding protein with a role in glycogen metabolism."
Printen J.A., Brady M.J., Saltiel A.R.
Science 275:1475-1478(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-317, FUNCTION, ASSOCIATION WITH GLYCOGEN, INTERACTION WITH PPP1CC.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 126-317.
Strain: C57BL/6J.
Tissue: Muellerian duct.
[4]"Role of protein targeting to glycogen (PTG) in the regulation of protein phosphatase-1 activity."
Brady M.J., Printen J.A., Mastick C.C., Saltiel A.R.
J. Biol. Chem. 272:20198-20204(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Overexpression of protein targeting to glycogen (PTG) in rat hepatocytes causes profound activation of glycogen synthesis independent of normal hormone- and substrate-mediated regulatory mechanisms."
Berman H.K., O'Doherty R.M., Anderson P., Newgard C.B.
J. Biol. Chem. 273:26421-26425(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Distinctive regulatory and metabolic properties of glycogen-targeting subunits of protein phosphatase-1 (PTG, GL, GM/RGl) expressed in hepatocytes."
Gasa R., Jensen P.B., Berman H.K., Brady M.J., DePaoli-Roach A.A., Newgard C.B.
J. Biol. Chem. 275:26396-26403(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Identification of binding sites on protein targeting to glycogen for enzymes of glycogen metabolism."
Fong N.M., Jensen T.C., Shah A.S., Parekh N.N., Saltiel A.R., Brady M.J.
J. Biol. Chem. 275:35034-35039(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF VAL-85; PHE-87; ASP-247 AND GLU-250.
[8]"Overexpression of protein targeting to glycogen in cultured human muscle cells stimulates glycogen synthesis independent of glycogen and glucose 6-phosphate levels."
Lerin C., Montell E., Berman H.K., Newgard C.B., Gomez-Foix A.M.
J. Biol. Chem. 275:39991-39995(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Activation of direct and indirect pathways of glycogen synthesis by hepatic overexpression of protein targeting to glycogen."
O'Doherty R.M., Jensen P.B., Anderson P., Jones J.G., Berman H.K., Kearney D., Newgard C.B.
J. Clin. Invest. 105:479-488(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Protein targeting to glycogen overexpression results in the specific enhancement of glycogen storage in 3T3-L1 adipocytes."
Greenberg C.C., Meredith K.N., Yan L., Brady M.J.
J. Biol. Chem. 278:30835-30842(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"PTG gene deletion causes impaired glycogen synthesis and developmental insulin resistance."
Crosson S.M., Khan A., Printen J., Pessin J.E., Saltiel A.R.
J. Clin. Invest. 111:1423-1432(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[12]"The glycogenic action of protein targeting to glycogen in hepatocytes involves multiple mechanisms including phosphorylase inactivation and glycogen synthase translocation."
Green A.R., Aiston S., Greenberg C.C., Freeman S., Poucher S.M., Brady M.J., Agius L.
J. Biol. Chem. 279:46474-46482(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Central role for protein targeting to glycogen in the maintenance of cellular glycogen stores in 3T3-L1 adipocytes."
Greenberg C.C., Danos A.M., Brady M.J.
Mol. Cell. Biol. 26:334-342(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC052769 mRNA. Translation: AAH52769.1.
BC054739 mRNA. Translation: AAH54739.1.
U89924 mRNA. Translation: AAB49689.1. Different initiation.
AK078506 mRNA. Translation: BAC37313.1.
CCDSCCDS37968.1.
RefSeqNP_058550.1. NM_016854.2.
UniGeneMm.24724.

3D structure databases

ProteinModelPortalQ7TMB3.
SMRQ7TMB3. Positions 134-262.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000084578.

Protein family/group databases

CAZyCBM21. Carbohydrate-Binding Module Family 21.

Proteomic databases

PaxDbQ7TMB3.
PRIDEQ7TMB3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000087321; ENSMUSP00000084578; ENSMUSG00000067279.
GeneID53412.
KEGGmmu:53412.
UCSCuc008hhs.1. mouse.

Organism-specific databases

CTD5507.
MGIMGI:1858229. Ppp1r3c.

Phylogenomic databases

eggNOGNOG288354.
GeneTreeENSGT00530000062978.
HOGENOMHOG000026799.
HOVERGENHBG052744.
InParanoidQ7TMB3.
KOK07189.
OMAIVHVQWK.
OrthoDBEOG7H4DTW.
PhylomeDBQ7TMB3.
TreeFamTF105537.

Gene expression databases

BgeeQ7TMB3.
GenevestigatorQ7TMB3.

Family and domain databases

InterProIPR005036. CBM_21.
IPR017434. Pase-1_Glycogen_target-su_met.
[Graphical view]
PfamPF03370. CBM_21. 1 hit.
[Graphical view]
PIRSFPIRSF038207. PP1_GT_animal. 1 hit.
PROSITEPS51159. CBM21. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio310221.
PROQ7TMB3.
SOURCESearch...

Entry information

Entry namePPR3C_MOUSE
AccessionPrimary (citable) accession number: Q7TMB3
Secondary accession number(s): O08541, Q8BJW8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 1, 2003
Last modified: July 9, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot