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Protein

Nucleoprotein

Gene

N

Organism
Rabies virus (strain ERA) (RABV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Encapsidates the genome in a ratio of one protein N per nine ribonucleotides, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it's own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation. The soluble N-P complex encapsidates specifically the genomic RNA, with protein N protecting the genome like a pearl necklace. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for viral transcription and replication. Protein N binds protein P in the NC through a different interaction, and can be phosphorylated. Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Superantigen

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Short name:
NP
Alternative name(s):
Nucleocapsid protein
Short name:
Protein N
Gene namesi
Name:N
OrganismiRabies virus (strain ERA) (RABV)
Taxonomic identifieri11295 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesRhabdoviridaeLyssavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Mammalia [TaxID: 40674]
Proteomesi
  • UP000008619 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450NucleoproteinPRO_0000295209Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei389 – 3891Phosphoserine; by host CK2By similarity

Post-translational modificationi

Phosphorylated by host CK2. Unphosphorylated protein N seems to have a better affinity for leader viral promoter encapsidation. Phosphorylation of protein N in ribonucleocapsid may stabilize the interaction with protein P, thereby playing an important role in viral transcription/replication (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. In nucleocapsid, binds protein P and thereby positions the polymerase on the template. Protein P acts as a chaperone on free protein N to prevent it from aggregation before encapsidating genomic RNA (By similarity).By similarity

Structurei

Secondary structure

1
450
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 143Combined sources
Helixi50 – 545Combined sources
Turni55 – 606Combined sources
Helixi66 – 7611Combined sources
Beta strandi100 – 1023Combined sources
Helixi134 – 15219Combined sources
Helixi158 – 17215Combined sources
Turni173 – 1753Combined sources
Beta strandi176 – 1783Combined sources
Turni189 – 1924Combined sources
Helixi201 – 21616Combined sources
Helixi222 – 2265Combined sources
Helixi229 – 2313Combined sources
Helixi237 – 24711Combined sources
Beta strandi248 – 2503Combined sources
Helixi256 – 2594Combined sources
Helixi265 – 2728Combined sources
Beta strandi275 – 2773Combined sources
Beta strandi279 – 2813Combined sources
Helixi285 – 2873Combined sources
Helixi289 – 2924Combined sources
Turni302 – 3054Combined sources
Helixi306 – 31813Combined sources
Helixi322 – 3254Combined sources
Beta strandi331 – 3333Combined sources
Helixi335 – 34915Combined sources
Beta strandi360 – 3623Combined sources
Helixi363 – 3708Combined sources
Helixi402 – 4109Combined sources
Turni411 – 4144Combined sources
Helixi418 – 42912Combined sources
Helixi438 – 4458Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GTTX-ray3.49A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V1-450[»]
ProteinModelPortaliQ7TBN9.
SMRiQ7TBN9. Positions 5-448.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7TBN9.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.3570.10. 1 hit.
1.10.3610.10. 1 hit.
InterProiIPR000448. Rhabdo_ncapsid.
IPR023331. Rhabdovirus_ncapsid_C.
IPR023330. Rhabdovirus_ncapsid_N.
[Graphical view]
PfamiPF00945. Rhabdo_ncap. 1 hit.
[Graphical view]
ProDomiPD002087. Rhabd_nucleocap. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF140809. SSF140809. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7TBN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDADKIVFKV NNQVVSLKPE IIVDQHEYKY PAIKDLKKPC ITLGKAPDLN
60 70 80 90 100
KAYKSVLSGM SAAKLDPDDV CSYLAAAMQF FEGTCPEDWT SYGIVIARKG
110 120 130 140 150
DKITPGSLVE IKRTDVEGNW ALTGGMELTR DPTVPEHASL VGLLLSLYRL
160 170 180 190 200
SKISGQNTGN YKTNIADRIE QIFETAPFVK IVEHHTLMTT HKMCANWSTI
210 220 230 240 250
PNFRFLAGTY DMFFSRIEHL YSAIRVGTVV TAYEDCSGLV SFTGFIKQIN
260 270 280 290 300
LTAREAILYF FHKNFEEEIR RMFEPGQETA VPHSYFIHFR SLGLSGKSPY
310 320 330 340 350
SSNAVGHVFN LIHFVGCYMG QVRSLNATVI AACAPHEMSV LGGYLGEEFF
360 370 380 390 400
GKGTFERRFF RDEKELQEYE AAELTKTDVA LADDGTVNSD DEDYFSGETR
410 420 430 440 450
SPEAVYTRIM MNGGRLKRSH IRRYVSVSSN HQARPNSFAE FLNKTYSSDS
Length:450
Mass (Da):50,578
Last modified:October 1, 2003 - v1
Checksum:i6B5A4419AAFCEE04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF406696 Genomic RNA. Translation: AAP81753.1.
EF206707 Genomic RNA. Translation: ABN11291.1.
EF206717 Genomic RNA. Translation: ABN11341.1.
EF206718 Genomic RNA. Translation: ABN11346.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF406696 Genomic RNA. Translation: AAP81753.1.
EF206707 Genomic RNA. Translation: ABN11291.1.
EF206717 Genomic RNA. Translation: ABN11341.1.
EF206718 Genomic RNA. Translation: ABN11346.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GTTX-ray3.49A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V1-450[»]
ProteinModelPortaliQ7TBN9.
SMRiQ7TBN9. Positions 5-448.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ7TBN9.

Family and domain databases

Gene3Di1.10.3570.10. 1 hit.
1.10.3610.10. 1 hit.
InterProiIPR000448. Rhabdo_ncapsid.
IPR023331. Rhabdovirus_ncapsid_C.
IPR023330. Rhabdovirus_ncapsid_N.
[Graphical view]
PfamiPF00945. Rhabdo_ncap. 1 hit.
[Graphical view]
ProDomiPD002087. Rhabd_nucleocap. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF140809. SSF140809. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Glycoprotein of nonpathogenic rabies viruses is a key determinant of human cell apoptosis."
    Prehaud C., Lay S., Dietzschold B., Lafon M.
    J. Virol. 77:10537-10547(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: CVS-2003.
  2. Prehaud C.J., Lay S.J.
    Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: CVS-2001.
  3. "Complete nucleotide sequencing of SAD derivatives of attenuated rabies virus vaccine strains."
    Geue L., Schares S., Schnick C., Kliemt J., Beckert A., Hoffmann B., Freuling C., Marston D., McElhinney L., Fooks A., Zanoni R., Peterhans E., Cox J., Mueller T.
    Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: ERA, Isolate SAD1-3670 var 1 and Isolate SAD1-3670 var 2.
  4. Cited for: X-RAY CRYSTALLOGRAPHY (3.49 ANGSTROMS).

Entry informationi

Entry nameiNCAP_RABVE
AccessioniPrimary (citable) accession number: Q7TBN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: October 1, 2003
Last modified: May 11, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Displays a superantigen activity in human and mouse, activating mostly V-beta-8 subtypes of T-cell receptor.By similarity

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.