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Q7T6Y8

- RIR1_MIMIV

UniProt

Q7T6Y8 - RIR1_MIMIV

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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene
RNR1, MIMI_R313
Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei271 – 2711Substrate By similarity
Sitei287 – 2871Important for hydrogen atom transfer By similarity
Sitei294 – 2941Allosteric effector binding By similarity
Binding sitei315 – 3151Substrate; via amide nitrogen By similarity
Sitei324 – 3241Allosteric effector binding By similarity
Active sitei493 – 4931Proton acceptor By similarity
Active sitei495 – 4951Cysteine radical intermediate By similarity
Active sitei497 – 4971Proton acceptor By similarity
Sitei510 – 5101Important for hydrogen atom transfer By similarity
Sitei809 – 8091Important for electron transfer By similarity
Sitei810 – 8101Important for electron transfer By similarity
Sitei876 – 8761Interacts with thioredoxin/glutaredoxin By similarity
Sitei879 – 8791Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
Name:RNR1
Ordered Locus Names:MIMI_R313
OrganismiAcanthamoeba polyphaga mimivirus (APMV)
Taxonomic identifieri212035 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
ProteomesiUP000001134: Genome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 881881Ribonucleoside-diphosphate reductase large subunitPRO_0000187229Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi287 ↔ 510Redox-active By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ7T6Y8.
SMRiQ7T6Y8. Positions 145-814.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 16092ATP-coneAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni286 – 2872Substrate binding By similarity
Regioni493 – 4975Substrate binding By similarity
Regioni675 – 6795Substrate binding By similarity

Sequence similaritiesi

Contains 1 ATP-cone domain.

Family and domain databases

InterProiIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7T6Y8-1 [UniParc]FASTAAdd to Basket

« Hide

MNSKNSIILD NLADVSDIID YEQNSIDYQR SIKNIEQLKS TEATEITNKL    50
AINKAKKSSN ILLEVPKETI YLDHNGSIQV ITSEIIRTYV EKIFEKMEVT 100
HLDIDKMTKN IFPKLKSINT IKDIDNQIIS TASEMVTDHY DYPKIAVWIL 150
MTNLHDNTSD DFLETAEKLY NNKSESNKNA PIISENIYNF IKKHIKEINR 200
VIRYDRDYNL TIFGFRTLEK SYLKRINKKI VERPQHLFMR VAITLHYRKN 250
DLDRIFETYK YLSKGYFTHA TPTLFNAGTT HEQLSSCFLL GIGDSLEEIS 300
ECWKECALIS KHAGGIGIHM TNIRVEGAYI ASTQGEAGGL RVLTIFNDIS 350
RYANQGGKRP GSFAIFIEPW HGDIFFFLDL KKNTGAETER ARDLFLGLMI 400
NDIFMERVYK DDVWSLMCPS QCPNLLNKYG DEFTKEYLKY ESEGIYLKQI 450
RAIDLWFKIM ESQIETGVPY VMFKDAINDK SNQINIGVVN GSNLCCEIVE 500
VSDKNNFSVC NLSSICLPRF VKIIDEVPTF NYQKLFKISR ILTRNLNNII 550
DINFYPLDKT RITNLRDRPI GIGVQGLADV FAMFKTPFDS EIARDLNRKI 600
FETIYYGALT ESNKLAREFG TYQTYQGSPI SQGKFQFDLW NFDKSQLSGM 650
WDWELLRQEI LNHGVRNSLV TTCMPTASTS QIMGWNECIE PYTENIYSRS 700
TMAGEYFVIN KHLIKDLIEL GVWNSEMVDL IKYYKGSVAN IPNIPDNIKA 750
IYRTVWEIPQ KSIIEMAADR APFVDQTQSM NLYIDKPSFA RLNSCLFYAW 800
RKGLKTGMYY LRSKPASSAN QFGIDIDKIK EIESKNGIKK QEDTFDLTDL 850
TNTINQQVSG MVCRFVPAHL RKPGECLSCD G 881
Length:881
Mass (Da):101,472
Last modified:December 7, 2004 - v2
Checksum:iBC4A893F2212E068
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY653733 Genomic DNA. Translation: AAQ09572.2.
RefSeqiYP_003986816.1. NC_014649.1.

Genome annotation databases

GeneIDi9924930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY653733 Genomic DNA. Translation: AAQ09572.2 .
RefSeqi YP_003986816.1. NC_014649.1.

3D structure databases

ProteinModelPortali Q7T6Y8.
SMRi Q7T6Y8. Positions 145-814.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 9924930.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS51161. ATP_CONE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rowbotham-Bradford.

Entry informationi

Entry nameiRIR1_MIMIV
AccessioniPrimary (citable) accession number: Q7T6Y8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 7, 2004
Last modified: May 14, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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