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Q7T6Y8

- RIR1_MIMIV

UniProt

Q7T6Y8 - RIR1_MIMIV

Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

RNR1

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 2 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Enzyme regulationi

    Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei271 – 2711SubstrateBy similarity
    Sitei287 – 2871Important for hydrogen atom transferBy similarity
    Sitei294 – 2941Allosteric effector bindingBy similarity
    Binding sitei315 – 3151Substrate; via amide nitrogenBy similarity
    Sitei324 – 3241Allosteric effector bindingBy similarity
    Active sitei493 – 4931Proton acceptorBy similarity
    Active sitei495 – 4951Cysteine radical intermediateBy similarity
    Active sitei497 – 4971Proton acceptorBy similarity
    Sitei510 – 5101Important for hydrogen atom transferBy similarity
    Sitei809 – 8091Important for electron transferBy similarity
    Sitei810 – 8101Important for electron transferBy similarity
    Sitei876 – 8761Interacts with thioredoxin/glutaredoxinBy similarity
    Sitei879 – 8791Interacts with thioredoxin/glutaredoxinBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase large subunit
    Gene namesi
    Name:RNR1
    Ordered Locus Names:MIMI_R313
    OrganismiAcanthamoeba polyphaga mimivirus (APMV)
    Taxonomic identifieri212035 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
    Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
    ProteomesiUP000001134: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. ribonucleoside-diphosphate reductase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 881881Ribonucleoside-diphosphate reductase large subunitPRO_0000187229Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi287 ↔ 510Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ7T6Y8.
    SMRiQ7T6Y8. Positions 145-814.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini69 – 16092ATP-conePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni286 – 2872Substrate bindingBy similarity
    Regioni493 – 4975Substrate bindingBy similarity
    Regioni675 – 6795Substrate bindingBy similarity

    Sequence similaritiesi

    Contains 1 ATP-cone domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR005144. ATP-cone.
    IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    IPR008926. RNR_R1-su_N.
    [Graphical view]
    PfamiPF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    SUPFAMiSSF48168. SSF48168. 1 hit.
    TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
    PROSITEiPS51161. ATP_CONE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7T6Y8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSKNSIILD NLADVSDIID YEQNSIDYQR SIKNIEQLKS TEATEITNKL    50
    AINKAKKSSN ILLEVPKETI YLDHNGSIQV ITSEIIRTYV EKIFEKMEVT 100
    HLDIDKMTKN IFPKLKSINT IKDIDNQIIS TASEMVTDHY DYPKIAVWIL 150
    MTNLHDNTSD DFLETAEKLY NNKSESNKNA PIISENIYNF IKKHIKEINR 200
    VIRYDRDYNL TIFGFRTLEK SYLKRINKKI VERPQHLFMR VAITLHYRKN 250
    DLDRIFETYK YLSKGYFTHA TPTLFNAGTT HEQLSSCFLL GIGDSLEEIS 300
    ECWKECALIS KHAGGIGIHM TNIRVEGAYI ASTQGEAGGL RVLTIFNDIS 350
    RYANQGGKRP GSFAIFIEPW HGDIFFFLDL KKNTGAETER ARDLFLGLMI 400
    NDIFMERVYK DDVWSLMCPS QCPNLLNKYG DEFTKEYLKY ESEGIYLKQI 450
    RAIDLWFKIM ESQIETGVPY VMFKDAINDK SNQINIGVVN GSNLCCEIVE 500
    VSDKNNFSVC NLSSICLPRF VKIIDEVPTF NYQKLFKISR ILTRNLNNII 550
    DINFYPLDKT RITNLRDRPI GIGVQGLADV FAMFKTPFDS EIARDLNRKI 600
    FETIYYGALT ESNKLAREFG TYQTYQGSPI SQGKFQFDLW NFDKSQLSGM 650
    WDWELLRQEI LNHGVRNSLV TTCMPTASTS QIMGWNECIE PYTENIYSRS 700
    TMAGEYFVIN KHLIKDLIEL GVWNSEMVDL IKYYKGSVAN IPNIPDNIKA 750
    IYRTVWEIPQ KSIIEMAADR APFVDQTQSM NLYIDKPSFA RLNSCLFYAW 800
    RKGLKTGMYY LRSKPASSAN QFGIDIDKIK EIESKNGIKK QEDTFDLTDL 850
    TNTINQQVSG MVCRFVPAHL RKPGECLSCD G 881
    Length:881
    Mass (Da):101,472
    Last modified:December 7, 2004 - v2
    Checksum:iBC4A893F2212E068
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY653733 Genomic DNA. Translation: AAQ09572.2.
    RefSeqiYP_003986816.1. NC_014649.1.

    Genome annotation databases

    GeneIDi9924930.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY653733 Genomic DNA. Translation: AAQ09572.2 .
    RefSeqi YP_003986816.1. NC_014649.1.

    3D structure databases

    ProteinModelPortali Q7T6Y8.
    SMRi Q7T6Y8. Positions 145-814.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 9924930.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    InterProi IPR005144. ATP-cone.
    IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    IPR008926. RNR_R1-su_N.
    [Graphical view ]
    Pfami PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    SUPFAMi SSF48168. SSF48168. 1 hit.
    TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
    PROSITEi PS51161. ATP_CONE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Rowbotham-Bradford.

    Entry informationi

    Entry nameiRIR1_MIMIV
    AccessioniPrimary (citable) accession number: Q7T6Y8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 65 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3