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Q7T6Y8

- RIR1_MIMIV

UniProt

Q7T6Y8 - RIR1_MIMIV

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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

RNR1

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei271 – 2711SubstrateBy similarity
Sitei287 – 2871Important for hydrogen atom transferBy similarity
Sitei294 – 2941Allosteric effector bindingBy similarity
Binding sitei315 – 3151Substrate; via amide nitrogenBy similarity
Sitei324 – 3241Allosteric effector bindingBy similarity
Active sitei493 – 4931Proton acceptorBy similarity
Active sitei495 – 4951Cysteine radical intermediateBy similarity
Active sitei497 – 4971Proton acceptorBy similarity
Sitei510 – 5101Important for hydrogen atom transferBy similarity
Sitei809 – 8091Important for electron transferBy similarity
Sitei810 – 8101Important for electron transferBy similarity
Sitei876 – 8761Interacts with thioredoxin/glutaredoxinBy similarity
Sitei879 – 8791Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
Name:RNR1
Ordered Locus Names:MIMI_R313
OrganismiAcanthamoeba polyphaga mimivirus (APMV)
Taxonomic identifieri212035 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
ProteomesiUP000001134: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 881881Ribonucleoside-diphosphate reductase large subunitPRO_0000187229Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi287 ↔ 510Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ7T6Y8.
SMRiQ7T6Y8. Positions 145-814.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 16092ATP-conePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni286 – 2872Substrate bindingBy similarity
Regioni493 – 4975Substrate bindingBy similarity
Regioni675 – 6795Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 ATP-cone domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7T6Y8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSKNSIILD NLADVSDIID YEQNSIDYQR SIKNIEQLKS TEATEITNKL
60 70 80 90 100
AINKAKKSSN ILLEVPKETI YLDHNGSIQV ITSEIIRTYV EKIFEKMEVT
110 120 130 140 150
HLDIDKMTKN IFPKLKSINT IKDIDNQIIS TASEMVTDHY DYPKIAVWIL
160 170 180 190 200
MTNLHDNTSD DFLETAEKLY NNKSESNKNA PIISENIYNF IKKHIKEINR
210 220 230 240 250
VIRYDRDYNL TIFGFRTLEK SYLKRINKKI VERPQHLFMR VAITLHYRKN
260 270 280 290 300
DLDRIFETYK YLSKGYFTHA TPTLFNAGTT HEQLSSCFLL GIGDSLEEIS
310 320 330 340 350
ECWKECALIS KHAGGIGIHM TNIRVEGAYI ASTQGEAGGL RVLTIFNDIS
360 370 380 390 400
RYANQGGKRP GSFAIFIEPW HGDIFFFLDL KKNTGAETER ARDLFLGLMI
410 420 430 440 450
NDIFMERVYK DDVWSLMCPS QCPNLLNKYG DEFTKEYLKY ESEGIYLKQI
460 470 480 490 500
RAIDLWFKIM ESQIETGVPY VMFKDAINDK SNQINIGVVN GSNLCCEIVE
510 520 530 540 550
VSDKNNFSVC NLSSICLPRF VKIIDEVPTF NYQKLFKISR ILTRNLNNII
560 570 580 590 600
DINFYPLDKT RITNLRDRPI GIGVQGLADV FAMFKTPFDS EIARDLNRKI
610 620 630 640 650
FETIYYGALT ESNKLAREFG TYQTYQGSPI SQGKFQFDLW NFDKSQLSGM
660 670 680 690 700
WDWELLRQEI LNHGVRNSLV TTCMPTASTS QIMGWNECIE PYTENIYSRS
710 720 730 740 750
TMAGEYFVIN KHLIKDLIEL GVWNSEMVDL IKYYKGSVAN IPNIPDNIKA
760 770 780 790 800
IYRTVWEIPQ KSIIEMAADR APFVDQTQSM NLYIDKPSFA RLNSCLFYAW
810 820 830 840 850
RKGLKTGMYY LRSKPASSAN QFGIDIDKIK EIESKNGIKK QEDTFDLTDL
860 870 880
TNTINQQVSG MVCRFVPAHL RKPGECLSCD G
Length:881
Mass (Da):101,472
Last modified:December 7, 2004 - v2
Checksum:iBC4A893F2212E068
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY653733 Genomic DNA. Translation: AAQ09572.2.
RefSeqiYP_003986816.1. NC_014649.1.

Genome annotation databases

GeneIDi9924930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY653733 Genomic DNA. Translation: AAQ09572.2 .
RefSeqi YP_003986816.1. NC_014649.1.

3D structure databases

ProteinModelPortali Q7T6Y8.
SMRi Q7T6Y8. Positions 145-814.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 9924930.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR005144. ATP-cone.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS51161. ATP_CONE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rowbotham-Bradford.

Entry informationi

Entry nameiRIR1_MIMIV
AccessioniPrimary (citable) accession number: Q7T6Y8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 7, 2004
Last modified: October 29, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3