##gff-version 3
Q7T6X9	UniProtKB	Chain	1	336	.	.	.	ID=PRO_0000145212;Note=DNA topoisomerase 1B	
Q7T6X9	UniProtKB	Domain	79	336	.	.	.	Note=Topo IB-type catalytic;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU01382	
Q7T6X9	UniProtKB	Active site	294	294	.	.	.	Note=O-(3'-phospho-DNA)-tyrosine intermediate;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU01382	
Q7T6X9	UniProtKB	Mutagenesis	135	135	.	.	.	Note=Complete loss of supercoil relaxation activity in vitro. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16352556;Dbxref=PMID:16352556	
Q7T6X9	UniProtKB	Mutagenesis	241	241	.	.	.	Note=Complete loss of supercoil relaxation activity in vitro. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16352556;Dbxref=PMID:16352556	
Q7T6X9	UniProtKB	Mutagenesis	285	285	.	.	.	Note=Partial loss of supercoil relaxation activity in vitro. H->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16352556;Dbxref=PMID:16352556	
Q7T6X9	UniProtKB	Mutagenesis	294	294	.	.	.	Note=Complete loss of supercoil relaxation activity in vitro. Y->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16352556;Dbxref=PMID:16352556