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Q7T6X9

- TOP1E_MIMIV

UniProt

Q7T6X9 - TOP1E_MIMIV

Protein

DNA topoisomerase 1B

Gene

TOP1E

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 2 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Cleaves DNA after CCCTT sequence.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei294 – 2941O-(3'-phospho-DNA)-tyrosine intermediateBy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-EC
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

    GO - Biological processi

    1. DNA topological change Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1B (EC:5.99.1.2)
    Short name:
    TopIB
    Alternative name(s):
    DNA topoisomerase type IB
    Gene namesi
    Name:TOP1E
    Ordered Locus Names:MIMI_R194
    OrganismiAcanthamoeba polyphaga mimivirus (APMV)
    Taxonomic identifieri212035 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
    Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
    ProteomesiUP000001134: Genome

    Subcellular locationi

    Virion 1 Publication

    GO - Cellular componenti

    1. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Virion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi135 – 1351R → A: Complete loss of supercoil relaxation activity in vitro. 1 Publication
    Mutagenesisi241 – 2411R → A: Complete loss of supercoil relaxation activity in vitro. 1 Publication
    Mutagenesisi285 – 2851H → A: Partial loss of supercoil relaxation activity in vitro. 1 Publication
    Mutagenesisi294 – 2941Y → A: Complete loss of supercoil relaxation activity in vitro. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336DNA topoisomerase 1BPRO_0000145212Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ7T6X9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the type IB topoisomerase family.Curated

    Family and domain databases

    Gene3Di1.20.120.380. 1 hit.
    3.30.66.10. 1 hit.
    3.90.15.10. 1 hit.
    InterProiIPR011010. DNA_brk_join_enz.
    IPR001631. TopoI.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR027362. TopoI_cat_dom2.
    IPR013500. TopoI_cat_euk.
    IPR015346. TopoI_N_vir.
    [Graphical view]
    PfamiPF01028. Topoisom_I. 1 hit.
    [Graphical view]
    PRINTSiPR00416. EUTPISMRASEI.
    SUPFAMiSSF56349. SSF56349. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7T6X9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYDEKHLME GIYREKSGDK FIYYYFDNNE EVTTKDIERI NKLRIPPAWT    50
    NVWVARDPNS PIQAIGTDSK GRKQYRYNEI HIQGAEKEKF KRLYDFIKSI 100
    PKLEKAMVRD NNFPFYNKNR VISLMLQMVR DYNMRVGKEV YARQNKSYGI 150
    SSLRKKHVKI SPGVITLNFK GKSGQRLNYT IRNDFYIDGI KMLMKLEGDR 200
    LFQYISTDED GNEKIMRVND RDLNKYIQEN MGSEFTIKDF RTFGANLYFI 250
    QALLSETRKR TPKNRKTIKK NIANAFKSTA RQLKHTGAVS KKSYVMNYTL 300
    ELYQNNPEFF IEHKNDDPID FLLRILKSYR KDVLGE 336
    Length:336
    Mass (Da):39,879
    Last modified:December 7, 2004 - v2
    Checksum:i3C0A8CDE8D274FAB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY653733 Genomic DNA. Translation: AAQ09581.2.
    RefSeqiYP_003986690.1. NC_014649.1.

    Genome annotation databases

    GeneIDi9924801.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY653733 Genomic DNA. Translation: AAQ09581.2 .
    RefSeqi YP_003986690.1. NC_014649.1.

    3D structure databases

    ProteinModelPortali Q7T6X9.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 9924801.

    Family and domain databases

    Gene3Di 1.20.120.380. 1 hit.
    3.30.66.10. 1 hit.
    3.90.15.10. 1 hit.
    InterProi IPR011010. DNA_brk_join_enz.
    IPR001631. TopoI.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR027362. TopoI_cat_dom2.
    IPR013500. TopoI_cat_euk.
    IPR015346. TopoI_N_vir.
    [Graphical view ]
    Pfami PF01028. Topoisom_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00416. EUTPISMRASEI.
    SUPFAMi SSF56349. SSF56349. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Rowbotham-Bradford.
    2. "Characterization of mimivirus DNA topoisomerase IB suggests horizontal gene transfer between eukaryal viruses and bacteria."
      Benarroch D., Claverie J.-M., Raoult D., Shuman S.
      J. Virol. 80:314-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, MUTAGENESIS OF ARG-135; ARG-241; HIS-285 AND TYR-294.
      Strain: Rowbotham-Bradford.
    3. "Mimivirus giant particles incorporate a large fraction of anonymous and unique gene products."
      Renesto P., Abergel C., Decloquement P., Moinier D., Azza S., Ogata H., Fourquet P., Gorvel J.-P., Claverie J.-M., Raoult D.
      J. Virol. 80:11678-11685(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiTOP1E_MIMIV
    AccessioniPrimary (citable) accession number: Q7T6X9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 61 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3