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Q7T6X9 (TOP1E_MIMIV) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1B

Short name=TopIB
EC=5.99.1.2
Alternative name(s):
DNA topoisomerase type IB
Gene names
Name:TOP1E
Ordered Locus Names:MIMI_R194
OrganismAcanthamoeba polyphaga mimivirus (APMV) [Reference proteome]
Taxonomic identifier212035 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Cleaves DNA after CCCTT sequence.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Subunit structure

Monomer By similarity.

Subcellular location

Virion.

Sequence similarities

Belongs to the type IB topoisomerase family.

Ontologies

Keywords
   Cellular componentVirion
   LigandDNA-binding
   Molecular functionIsomerase
Topoisomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA topological change

Inferred from electronic annotation. Source: InterPro

   Cellular_componentvirion

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase type I activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA topoisomerase type II (ATP-hydrolyzing) activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336DNA topoisomerase 1B
PRO_0000145212

Sites

Active site2941O-(3'-phospho-DNA)-tyrosine intermediate By similarity

Experimental info

Mutagenesis1351R → A: Complete loss of supercoil relaxation activity in vitro. Ref.2
Mutagenesis2411R → A: Complete loss of supercoil relaxation activity in vitro. Ref.2
Mutagenesis2851H → A: Partial loss of supercoil relaxation activity in vitro. Ref.2
Mutagenesis2941Y → A: Complete loss of supercoil relaxation activity in vitro. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q7T6X9 [UniParc].

Last modified December 7, 2004. Version 2.
Checksum: 3C0A8CDE8D274FAB

FASTA33639,879
        10         20         30         40         50         60 
MSYDEKHLME GIYREKSGDK FIYYYFDNNE EVTTKDIERI NKLRIPPAWT NVWVARDPNS 

        70         80         90        100        110        120 
PIQAIGTDSK GRKQYRYNEI HIQGAEKEKF KRLYDFIKSI PKLEKAMVRD NNFPFYNKNR 

       130        140        150        160        170        180 
VISLMLQMVR DYNMRVGKEV YARQNKSYGI SSLRKKHVKI SPGVITLNFK GKSGQRLNYT 

       190        200        210        220        230        240 
IRNDFYIDGI KMLMKLEGDR LFQYISTDED GNEKIMRVND RDLNKYIQEN MGSEFTIKDF 

       250        260        270        280        290        300 
RTFGANLYFI QALLSETRKR TPKNRKTIKK NIANAFKSTA RQLKHTGAVS KKSYVMNYTL 

       310        320        330 
ELYQNNPEFF IEHKNDDPID FLLRILKSYR KDVLGE 

« Hide

References

« Hide 'large scale' references
[1]"The 1.2-megabase genome sequence of Mimivirus."
Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M.
Science 306:1344-1350(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Rowbotham-Bradford.
[2]"Characterization of mimivirus DNA topoisomerase IB suggests horizontal gene transfer between eukaryal viruses and bacteria."
Benarroch D., Claverie J.-M., Raoult D., Shuman S.
J. Virol. 80:314-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, MUTAGENESIS OF ARG-135; ARG-241; HIS-285 AND TYR-294.
Strain: Rowbotham-Bradford.
[3]"Mimivirus giant particles incorporate a large fraction of anonymous and unique gene products."
Renesto P., Abergel C., Decloquement P., Moinier D., Azza S., Ogata H., Fourquet P., Gorvel J.-P., Claverie J.-M., Raoult D.
J. Virol. 80:11678-11685(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY653733 Genomic DNA. Translation: AAQ09581.2.
RefSeqYP_003986690.1. NC_014649.1.

3D structure databases

ProteinModelPortalQ7T6X9.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID9924801.

Family and domain databases

Gene3D1.20.120.380. 1 hit.
3.30.66.10. 1 hit.
3.90.15.10. 1 hit.
InterProIPR011010. DNA_brk_join_enz.
IPR001631. TopoI.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR027362. TopoI_cat_dom2.
IPR013500. TopoI_cat_euk.
IPR015346. TopoI_N_vir.
[Graphical view]
PfamPF01028. Topoisom_I. 1 hit.
[Graphical view]
PRINTSPR00416. EUTPISMRASEI.
SUPFAMSSF56349. SSF56349. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTOP1E_MIMIV
AccessionPrimary (citable) accession number: Q7T6X9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 7, 2004
Last modified: July 9, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families