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Protein

DNA topoisomerase 1B

Gene

TOP1E

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Cleaves DNA after CCCTT sequence.By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei294 – 2941O-(3'-phospho-DNA)-tyrosine intermediateBy similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-EC
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

GO - Biological processi

  1. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1B (EC:5.99.1.2)
Short name:
TopIB
Alternative name(s):
DNA topoisomerase type IB
Gene namesi
Name:TOP1E
Ordered Locus Names:MIMI_R194
OrganismiAcanthamoeba polyphaga mimivirus (APMV)
Taxonomic identifieri212035 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
ProteomesiUP000001134 Componenti: Genome

Subcellular locationi

Virion 1 Publication

GO - Cellular componenti

  1. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi135 – 1351R → A: Complete loss of supercoil relaxation activity in vitro. 1 Publication
Mutagenesisi241 – 2411R → A: Complete loss of supercoil relaxation activity in vitro. 1 Publication
Mutagenesisi285 – 2851H → A: Partial loss of supercoil relaxation activity in vitro. 1 Publication
Mutagenesisi294 – 2941Y → A: Complete loss of supercoil relaxation activity in vitro. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336DNA topoisomerase 1BPRO_0000145212Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the type IB topoisomerase family.Curated

Phylogenomic databases

KOiK03168.

Family and domain databases

Gene3Di1.20.120.380. 1 hit.
3.30.66.10. 1 hit.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR001631. TopoI.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR027362. TopoI_cat_dom2.
IPR013500. TopoI_cat_euk.
IPR015346. TopoI_N_vir.
[Graphical view]
PfamiPF01028. Topoisom_I. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SUPFAMiSSF56349. SSF56349. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7T6X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYDEKHLME GIYREKSGDK FIYYYFDNNE EVTTKDIERI NKLRIPPAWT
60 70 80 90 100
NVWVARDPNS PIQAIGTDSK GRKQYRYNEI HIQGAEKEKF KRLYDFIKSI
110 120 130 140 150
PKLEKAMVRD NNFPFYNKNR VISLMLQMVR DYNMRVGKEV YARQNKSYGI
160 170 180 190 200
SSLRKKHVKI SPGVITLNFK GKSGQRLNYT IRNDFYIDGI KMLMKLEGDR
210 220 230 240 250
LFQYISTDED GNEKIMRVND RDLNKYIQEN MGSEFTIKDF RTFGANLYFI
260 270 280 290 300
QALLSETRKR TPKNRKTIKK NIANAFKSTA RQLKHTGAVS KKSYVMNYTL
310 320 330
ELYQNNPEFF IEHKNDDPID FLLRILKSYR KDVLGE
Length:336
Mass (Da):39,879
Last modified:December 7, 2004 - v2
Checksum:i3C0A8CDE8D274FAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653733 Genomic DNA. Translation: AAQ09581.2.
RefSeqiYP_003986690.1. NC_014649.1.

Genome annotation databases

GeneIDi9924801.
KEGGivg:9924801.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653733 Genomic DNA. Translation: AAQ09581.2.
RefSeqiYP_003986690.1. NC_014649.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9924801.
KEGGivg:9924801.

Phylogenomic databases

KOiK03168.

Family and domain databases

Gene3Di1.20.120.380. 1 hit.
3.30.66.10. 1 hit.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR001631. TopoI.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR027362. TopoI_cat_dom2.
IPR013500. TopoI_cat_euk.
IPR015346. TopoI_N_vir.
[Graphical view]
PfamiPF01028. Topoisom_I. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SUPFAMiSSF56349. SSF56349. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rowbotham-Bradford.
  2. "Characterization of mimivirus DNA topoisomerase IB suggests horizontal gene transfer between eukaryal viruses and bacteria."
    Benarroch D., Claverie J.-M., Raoult D., Shuman S.
    J. Virol. 80:314-321(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS OF ARG-135; ARG-241; HIS-285 AND TYR-294.
    Strain: Rowbotham-Bradford.
  3. "Mimivirus giant particles incorporate a large fraction of anonymous and unique gene products."
    Renesto P., Abergel C., Decloquement P., Moinier D., Azza S., Ogata H., Fourquet P., Gorvel J.-P., Claverie J.-M., Raoult D.
    J. Virol. 80:11678-11685(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTOP1E_MIMIV
AccessioniPrimary (citable) accession number: Q7T6X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 7, 2004
Last modified: April 1, 2015
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.