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Q7T6X9

- TOP1E_MIMIV

UniProt

Q7T6X9 - TOP1E_MIMIV

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Protein

DNA topoisomerase 1B

Gene
TOP1E, MIMI_R194
Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Cleaves DNA after CCCTT sequence.

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei294 – 2941O-(3'-phospho-DNA)-tyrosine intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-EC
  4. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

GO - Biological processi

  1. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1B (EC:5.99.1.2)
Short name:
TopIB
Alternative name(s):
DNA topoisomerase type IB
Gene namesi
Name:TOP1E
Ordered Locus Names:MIMI_R194
OrganismiAcanthamoeba polyphaga mimivirus (APMV)
Taxonomic identifieri212035 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
ProteomesiUP000001134: Genome

Subcellular locationi

GO - Cellular componenti

  1. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi135 – 1351R → A: Complete loss of supercoil relaxation activity in vitro. 1 Publication
Mutagenesisi241 – 2411R → A: Complete loss of supercoil relaxation activity in vitro. 1 Publication
Mutagenesisi285 – 2851H → A: Partial loss of supercoil relaxation activity in vitro. 1 Publication
Mutagenesisi294 – 2941Y → A: Complete loss of supercoil relaxation activity in vitro. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336DNA topoisomerase 1BPRO_0000145212Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ7T6X9.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.20.120.380. 1 hit.
3.30.66.10. 1 hit.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR001631. TopoI.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR027362. TopoI_cat_dom2.
IPR013500. TopoI_cat_euk.
IPR015346. TopoI_N_vir.
[Graphical view]
PfamiPF01028. Topoisom_I. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SUPFAMiSSF56349. SSF56349. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7T6X9-1 [UniParc]FASTAAdd to Basket

« Hide

MSYDEKHLME GIYREKSGDK FIYYYFDNNE EVTTKDIERI NKLRIPPAWT    50
NVWVARDPNS PIQAIGTDSK GRKQYRYNEI HIQGAEKEKF KRLYDFIKSI 100
PKLEKAMVRD NNFPFYNKNR VISLMLQMVR DYNMRVGKEV YARQNKSYGI 150
SSLRKKHVKI SPGVITLNFK GKSGQRLNYT IRNDFYIDGI KMLMKLEGDR 200
LFQYISTDED GNEKIMRVND RDLNKYIQEN MGSEFTIKDF RTFGANLYFI 250
QALLSETRKR TPKNRKTIKK NIANAFKSTA RQLKHTGAVS KKSYVMNYTL 300
ELYQNNPEFF IEHKNDDPID FLLRILKSYR KDVLGE 336
Length:336
Mass (Da):39,879
Last modified:December 7, 2004 - v2
Checksum:i3C0A8CDE8D274FAB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY653733 Genomic DNA. Translation: AAQ09581.2.
RefSeqiYP_003986690.1. NC_014649.1.

Genome annotation databases

GeneIDi9924801.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY653733 Genomic DNA. Translation: AAQ09581.2 .
RefSeqi YP_003986690.1. NC_014649.1.

3D structure databases

ProteinModelPortali Q7T6X9.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 9924801.

Family and domain databases

Gene3Di 1.20.120.380. 1 hit.
3.30.66.10. 1 hit.
3.90.15.10. 1 hit.
InterProi IPR011010. DNA_brk_join_enz.
IPR001631. TopoI.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR027362. TopoI_cat_dom2.
IPR013500. TopoI_cat_euk.
IPR015346. TopoI_N_vir.
[Graphical view ]
Pfami PF01028. Topoisom_I. 1 hit.
[Graphical view ]
PRINTSi PR00416. EUTPISMRASEI.
SUPFAMi SSF56349. SSF56349. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rowbotham-Bradford.
  2. "Characterization of mimivirus DNA topoisomerase IB suggests horizontal gene transfer between eukaryal viruses and bacteria."
    Benarroch D., Claverie J.-M., Raoult D., Shuman S.
    J. Virol. 80:314-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS OF ARG-135; ARG-241; HIS-285 AND TYR-294.
    Strain: Rowbotham-Bradford.
  3. "Mimivirus giant particles incorporate a large fraction of anonymous and unique gene products."
    Renesto P., Abergel C., Decloquement P., Moinier D., Azza S., Ogata H., Fourquet P., Gorvel J.-P., Claverie J.-M., Raoult D.
    J. Virol. 80:11678-11685(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTOP1E_MIMIV
AccessioniPrimary (citable) accession number: Q7T6X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 7, 2004
Last modified: July 9, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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