Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA topoisomerase 1B

Gene

TOP1E

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Cleaves DNA after CCCTT sequence.By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei294O-(3'-phospho-DNA)-tyrosine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1B (EC:5.99.1.2)
Short name:
TopIB
Alternative name(s):
DNA topoisomerase type IB
Gene namesi
Name:TOP1E
Ordered Locus Names:MIMI_R194
OrganismiAcanthamoeba polyphaga mimivirus (APMV)
Taxonomic identifieri212035 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
Proteomesi
  • UP000001134 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • virion Source: UniProtKB-SubCell

Keywords - Cellular componenti

Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi135R → A: Complete loss of supercoil relaxation activity in vitro. 1 Publication1
Mutagenesisi241R → A: Complete loss of supercoil relaxation activity in vitro. 1 Publication1
Mutagenesisi285H → A: Partial loss of supercoil relaxation activity in vitro. 1 Publication1
Mutagenesisi294Y → A: Complete loss of supercoil relaxation activity in vitro. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001452121 – 336DNA topoisomerase 1BAdd BLAST336

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ7T6X9
SMRiQ7T6X9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the type IB topoisomerase family.Curated

Phylogenomic databases

OrthoDBiVOG090000FB

Family and domain databases

CDDicd00659 Topo_IB_C, 1 hit
Gene3Di3.30.66.10, 1 hit
3.90.15.10, 1 hit
InterProiView protein in InterPro
IPR011010 DNA_brk_join_enz
IPR035447 DNA_topo_I_N
IPR001631 TopoI
IPR014711 TopoI_cat_a-hlx-sub_euk
IPR013500 TopoI_cat_euk
PfamiView protein in Pfam
PF01028 Topoisom_I, 1 hit
PRINTSiPR00416 EUTPISMRASEI
SUPFAMiSSF55869 SSF55869, 1 hit
SSF56349 SSF56349, 1 hit

Sequencei

Sequence statusi: Complete.

Q7T6X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYDEKHLME GIYREKSGDK FIYYYFDNNE EVTTKDIERI NKLRIPPAWT
60 70 80 90 100
NVWVARDPNS PIQAIGTDSK GRKQYRYNEI HIQGAEKEKF KRLYDFIKSI
110 120 130 140 150
PKLEKAMVRD NNFPFYNKNR VISLMLQMVR DYNMRVGKEV YARQNKSYGI
160 170 180 190 200
SSLRKKHVKI SPGVITLNFK GKSGQRLNYT IRNDFYIDGI KMLMKLEGDR
210 220 230 240 250
LFQYISTDED GNEKIMRVND RDLNKYIQEN MGSEFTIKDF RTFGANLYFI
260 270 280 290 300
QALLSETRKR TPKNRKTIKK NIANAFKSTA RQLKHTGAVS KKSYVMNYTL
310 320 330
ELYQNNPEFF IEHKNDDPID FLLRILKSYR KDVLGE
Length:336
Mass (Da):39,879
Last modified:December 7, 2004 - v2
Checksum:i3C0A8CDE8D274FAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653733 Genomic DNA Translation: AAQ09581.2
RefSeqiYP_003986690.1, NC_014649.1

Genome annotation databases

GeneIDi9924801
KEGGivg:9924801

Similar proteinsi

Entry informationi

Entry nameiTOP1E_MIMIV
AccessioniPrimary (citable) accession number: Q7T6X9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 7, 2004
Last modified: February 28, 2018
This is version 74 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health