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Protein

Probable glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

MIMI_L619

Organism
Acanthamoeba polyphaga mimivirus (APMV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for glycosylation of proteins (Potential).Curated

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Pathway:iUDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable glutamine--fructose-6-phosphate aminotransferase [isomerizing] (MIMI_L619)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00113; UER00528.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
D-fructose-6-phosphate amidotransferase
Hexosephosphate aminotransferase
Gene namesi
Ordered Locus Names:MIMI_L619
OrganismiAcanthamoeba polyphaga mimivirus (APMV)
Taxonomic identifieri212035 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageMimiviridaeMimivirus
Virus hostiAcanthamoeba polyphaga (Amoeba) [TaxID: 5757]
ProteomesiUP000001134 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by hostBy similarity
Chaini2 – 606605Probable glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135286Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ7T6X6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 224223Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini282 – 427146SIS 1PROSITE-ProRule annotationAdd
BLAST
Domaini458 – 596139SIS 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

KOiK00820.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7T6X6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGISACLNH TNNSAMTSVV NALTKLQNRG YDSAGICTTS NGKFNFVKSV
60 70 80 90 100
SDDTNNAIHY IKNNPLANHH CSIAIGHTRW ATHGEKTIEN AHPHFDASGR
110 120 130 140 150
FSLIHNGIIE NYDQIKSMLV ESQNYQFYGQ TDTEVAVAYL SYLLSENKTW
160 170 180 190 200
FDFNESLKGS WAIIALDKFN PEKLYFMRNG SPLIIGFNET NTKAMIVSEL
210 220 230 240 250
SGFDSDISQY CIVGDNDYGY ITNNNDKYII KSQQHYQMIS MGKIVMDLTP
260 270 280 290 300
SPYKHWTQRE IYDQPNAIHS LITERIVDSQ LFFPEFNTIN FTLVEHIVLL
310 320 330 340 350
GCGTSYHAAQ IGRRYIREFR PNITVDVIDG ADFEETDIPK SRNTLLILLS
360 370 380 390 400
QSGETKDLYR ALVIGKQHSL KTIGIINVEN SLIAREVDTV LYLRAGRENA
410 420 430 440 450
VASTKSFTNQ VLMLFMLALK INLSLDNSQL DYYTMSLNNF PIEFKKIIDQ
460 470 480 490 500
SVNEIPKLLE FFDNQTSCFI LGKFGLEWIA KEGSLKIKEI SYVHSEGYSS
510 520 530 540 550
AALKHGPFAL LHQNIPVVLL ANDDSYFSKI ENANSEIRSR KAKVIFITNK
560 570 580 590 600
LIDNHCTDYL IHINTKSPLF HLLCIVPLQL LAYHLALSKG INPDYPRNLA

KVVTVE
Length:606
Mass (Da):68,570
Last modified:January 23, 2007 - v4
Checksum:i9C0644BDC1C80CFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653733 Genomic DNA. Translation: AAQ09584.2.
RefSeqiYP_003987136.1. NC_014649.1.

Genome annotation databases

GeneIDi9925259.
KEGGivg:9925259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653733 Genomic DNA. Translation: AAQ09584.2.
RefSeqiYP_003987136.1. NC_014649.1.

3D structure databases

ProteinModelPortaliQ7T6X6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9925259.
KEGGivg:9925259.

Phylogenomic databases

KOiK00820.

Enzyme and pathway databases

UniPathwayiUPA00113; UER00528.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rowbotham-Bradford.

Entry informationi

Entry nameiGFAT_MIMIV
AccessioniPrimary (citable) accession number: Q7T6X6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 23, 2007
Last modified: May 27, 2015
This is version 62 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.