Reviewed, UniProtKB/Swiss-Prot Q7T6X5 (RPO1_MIMIV)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase subunit 1
    EC=2.7.7.6

Gene names

Name:	RPO1
Ordered Locus Names:	MIMI_R501

Organism

Acanthamoeba polyphaga mimivirus (APMV) [Complete proteome]

Taxonomic identifier

212035 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Mimiviridae › Mimivirus

Virus host

Acanthamoeba polyphaga (Amoeba) [TaxID: 5757]

Protein attributes

Sequence length	1495 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Virion. Ref.2
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Virion
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1495	1495	DNA-directed RNA polymerase subunit 1		PRO_0000073917

Sequences

	Sequence		Length	Mass (Da)
	Q7T6X5-1 [UniParc]. Last modified December 7, 2004. Version 2. Checksum: 9439FE6F7B1A91DF	FASTA	1,495	169,839
	10 20 30 40 50 60 MEANKNTYSR LGDTIETVER IEFCINSNES IIRHSAIVDP NGITEAETFN SNNNEPVQGG 70 80 90 100 110 120 VIDKRLGVTE SHLECSTCGE TALRCPGHFG HIKFVEPVFH MGYLIYLKHI LSCICIRCNK 130 140 150 160 170 180 LLVYKNEKEI AALIKNKQGK QRFAEIRSIC KKVTHCQKEN YGCGTPAHKI SIDKRNGNIF 190 200 210 220 230 240 LLAEPVKRTD EYDETGETRK RPQQILTPQL CYDILKSVSD EDCIIMGFDP AKSRPEDMII 250 260 270 280 290 300 LNFPVPPVQV RPSIRAEILS SPTMDDDLTH KLIDIIKSNE NLKNTKGDGS LIKYTSINDD 310 320 330 340 350 360 FMLLQLHVAT FFANDMAGLA RSQQKNKKVT KSMSERLRGK EGRIRGNLMG KRVDMSARTV 370 380 390 400 410 420 ITSDPNIALN EVGVPLIIAK NLTFDEIVTE HNIEYLTQLV KNGKRVYPGA NFVIKHVIDA 430 440 450 460 470 480 EGNESGHIYH LKYVDKPISL KPGDIVKRQL IDGDIVIFNR QPSLHKLSMM GHKCHVIPDN 490 500 510 520 530 540 NLLTFRVNVS VTDPYNADFD GDEMNLHVPQ SIQTATEILL IANASRRFVS PATSNIAIKA 550 560 570 580 590 600 KQDTLMGSYV QTEPDMEIDW RDAMSILMST SVKLDNDIPK YQNVSGKFLY SQIIPEGLNI 610 620 630 640 650 660 TKRKNDKEFQ LKIKNGELTD GTLGKSEISS ILQRIWFQYG SKETQEFIDD AQRMILQFLM 670 680 690 700 710 720 RYGYTVSIKD TVIGEKVNQY IYDLIETKRK ETLAFITEYD NDPYVMTKDA FEIKLQENLK 730 740 750 760 770 780 SVQDEIKNTV MRNFDKNSGI FIAISSGSSG EPMNAGQIAG CIGQVIVEGK RIQIRFNGRT 790 800 810 820 830 840 LPMFPKFDDS AFSRGFCRNS FIEGLGPFEF FFQVMAGREG IINTAIKTAD TGYIQRKLVK 850 860 870 880 890 900 MLEDIKQEYD GTVRNANGKL ISCVYGDNGI NTENQVDQKI DLISANDNKV RNDYVYTEDE 910 920 930 940 950 960 IKYLIKNHKT DKRYTTDLNN SLYRKLISMR DQLRRIQRLV NLTSAEFKET YKMPVDIQQF 970 980 990 1000 1010 1020 IFNIINRDVR NNNVVVDPYY VLKMIKDMYY GSDSKIMKYN NRTSRIKKED EKRIKFLMKI 1030 1040 1050 1060 1070 1080 YLYDVLAPKK CTHVYKFSKQ EFDEIVDYFK KTIMLAKVEG GEMVGFVAAQ SIGEPVTQTN 1090 1100 1110 1120 1130 1140 LKSFHKSGTG KTVSGGLVRV KELLGISKNI KTPITEIILE EKYKNDKITA SRIASYLKYT 1150 1160 1170 1180 1190 1200 TLRDVVEKAD VIYDPEPFSK DGLMKKDGVD NIFDQEQGKT GCQTDIKNLP WVLRIMLSKE 1210 1220 1230 1240 1250 1260 KMIERNINML EIKTSFCRNW GTRNEDKTSK KEFNKVIDKI TQCAIVTNYD NSQVPIVHVR 1270 1280 1290 1300 1310 1320 FDANNYNLNT LIQFQEMVIN TYKIKGISNI TESNNIIEES YVDFDDEGNV VKKKQYVIIA 1330 1340 1350 1360 1370 1380 EGINLSEMSQ INGIDLLRTK CNDIVTIYEM YGVEAARTAF IKEFTAAIES SGGFSNYQHI 1390 1400 1410 1420 1430 1440 EILADAITHM GGLIPVNRHG ANKLDTDPFS RASFEKTVEQ LLAAAVFGES DHMRSVSARI 1450 1460 1470 1480 1490 MVGALINGGT GCFDLLLDHK KIQQSLVESE EVVAPVVPIK KKTVLDDLIS KKKSK

References

[1]	"The 1.2-megabase genome sequence of Mimivirus." Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M. Science 306:1344-1350(2004) [PubMed: 15486256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rowbotham-Bradford.
[2]	"Mimivirus giant particles incorporate a large fraction of anonymous and unique gene products." Renesto P., Abergel C., Decloquement P., Moinier D., Azza S., Ogata H., Fourquet P., Gorvel J.-P., Claverie J.-M., Raoult D. J. Virol. 80:11678-11685(2006) [PubMed: 16971431] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY653733 Genomic DNA. Translation: AAQ09585.2.
RefSeq	YP_142855.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3162568.
Enzyme and pathway databases
BRENDA	2.7.7.6. 281366.
Family and domain databases
InterPro	IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. IPR007073. RNA_pol_Rpb1_7. [Graphical view]
Gene3D	G3DSA:2.40.40.30. RNA_pol_A. 1 hit. G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit. G3DSA:3.30.1360.90. RNA_pol_Rpb1_7. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPO1_MIMIV

Accession

Primary (citable) accession number: Q7T6X5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 7, 2004
Last modified:	June 16, 2009
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)