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Protein

Lariat debranching enzyme

Gene

dbr1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via an RNA lariat intermediate in cDNA synthesis (By similarity).By similarity

Catalytic activityi

RNA processing activity that hydrolyzes the 2'-5' phosphodiester linkage at the branchpoint of excised intron lariats.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

mRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Lariat debranching enzyme (EC:3.1.-.-)
Gene namesi
Name:dbr1
ORF Names:zgc:63930
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-030131-2466. dbr1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 568568Lariat debranching enzymePRO_0000250361Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei478 – 4781Phosphothreonine1 Publication
Modified residuei568 – 5681Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7T3E4.
PRIDEiQ7T3E4.

PTM databases

iPTMnetiQ7T3E4.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000073957.

Structurei

3D structure databases

ProteinModelPortaliQ7T3E4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the lariat debranching enzyme family.Curated

Phylogenomic databases

eggNOGiKOG2863. Eukaryota.
ENOG410XP0F. LUCA.
HOGENOMiHOG000216468.
HOVERGENiHBG079917.
InParanoidiQ7T3E4.
KOiK18328.
PhylomeDBiQ7T3E4.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR007708. DBR1_C.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF05011. DBR1. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
SMARTiSM01124. DBR1. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7T3E4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVAVEGCCH GELDKIYESI SYLENKDGVK VDLLLCCGDF QAVRNEGDMK
60 70 80 90 100
CMAVPAKYRH MQTFYKYYSG EKKAPVLTIF IGGNHEASNH LQELPYGGWV
110 120 130 140 150
APNIYYLGYA GVIRYKGVRI GGLSGIFKSH DFKKGHFEFP PYNPETLRSV
160 170 180 190 200
YHIRNIDVFK LKQIKMPIDI FMTHDWPRGI YHYGNTNALL RQKKFLRQEV
210 220 230 240 250
ESSTLGSPAA ADLLEHLQPS YWFSAHLHVK FAALMQHEAK NNTAPKITKF
260 270 280 290 300
LSLDKCLPHR DFLQIVEVAD RPGSSEQLEY DPEWLAILKA TDNLQKPTCN
310 320 330 340 350
FWNPPQDNGL HSRWDFSASE EAMMEVVSDL SGDLCIPENF SLTVPPYDPS
360 370 380 390 400
QPQPHALPAY STNPQTTELC ATLNLTDIYI LAGQSGQIYG ERGGKGATEE
410 420 430 440 450
EDEEDSTGSA DEPSDFPSDT SGLSSSYNPD EITIEDEWEE EEDGGVGCGE
460 470 480 490 500
GKGMDAVVPE GQVGSQDSDR DSSPQRETAK RLILPAPCAK PKTEAPLHSL
510 520 530 540 550
PRLSLPPPSA CVSQGSSEEE GAFTAARVPK RTSGETTQSS AGQTGGTPQI
560
KRRNQSIYTA VEDEESDS
Length:568
Mass (Da):62,889
Last modified:October 1, 2003 - v1
Checksum:iED592C5D4416C480
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC053153 mRNA. Translation: AAH53153.1.
RefSeqiNP_955947.1. NM_199653.1.
UniGeneiDr.77975.

Genome annotation databases

GeneIDi323746.
KEGGidre:323746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC053153 mRNA. Translation: AAH53153.1.
RefSeqiNP_955947.1. NM_199653.1.
UniGeneiDr.77975.

3D structure databases

ProteinModelPortaliQ7T3E4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000073957.

PTM databases

iPTMnetiQ7T3E4.

Proteomic databases

PaxDbiQ7T3E4.
PRIDEiQ7T3E4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi323746.
KEGGidre:323746.

Organism-specific databases

CTDi51163.
ZFINiZDB-GENE-030131-2466. dbr1.

Phylogenomic databases

eggNOGiKOG2863. Eukaryota.
ENOG410XP0F. LUCA.
HOGENOMiHOG000216468.
HOVERGENiHBG079917.
InParanoidiQ7T3E4.
KOiK18328.
PhylomeDBiQ7T3E4.

Miscellaneous databases

PROiQ7T3E4.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR007708. DBR1_C.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF05011. DBR1. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
SMARTiSM01124. DBR1. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  2. "Online automated in vivo zebrafish phosphoproteomics: from large-scale analysis down to a single embryo."
    Lemeer S., Pinkse M.W.H., Mohammed S., van Breukelen B., den Hertog J., Slijper M., Heck A.J.R.
    J. Proteome Res. 7:1555-1564(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-478 AND SER-568, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiDBR1_DANRE
AccessioniPrimary (citable) accession number: Q7T3E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: October 1, 2003
Last modified: June 8, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.