Q7T310 (PIGM_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GPI mannosyltransferase 1
EC=2.4.1.-

Alternative name(s):
GPI mannosyltransferase I
Short name=GPI-MT-I
Phosphatidylinositol-glycan biosynthesis class M protein
Short name=PIG-M

Gene names

Name:	pigm
ORF Names:	zgc:64177

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly By similarity.
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the PIGM family.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	GPI mannosyltransferase 1		PRO_0000246219
Regions
	Topological domain	1 – 4	4	Cytoplasmic Potential
	Transmembrane	5 – 25	21	Helical; Potential
	Topological domain	26 – 65	40	Lumenal Potential
	Transmembrane	66 – 86	21	Helical; Potential
	Topological domain	87 – 125	39	Cytoplasmic Potential
	Transmembrane	126 – 148	23	Helical; Potential
	Topological domain	149 – 156	8	Lumenal Potential
	Transmembrane	157 – 177	21	Helical; Potential
	Topological domain	178 – 198	21	Cytoplasmic Potential
	Transmembrane	199 – 219	21	Helical; Potential
	Topological domain	220 – 261	42	Lumenal Potential
	Transmembrane	262 – 282	21	Helical; Potential
	Topological domain	283 – 302	20	Cytoplasmic Potential
	Transmembrane	303 – 323	21	Helical; Potential
	Topological domain	324 – 330	7	Lumenal Potential
	Transmembrane	331 – 351	21	Helical; Potential
	Topological domain	352 – 360	9	Cytoplasmic Potential
	Transmembrane	361 – 381	21	Helical; Potential
	Topological domain	382 – 392	11	Lumenal Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7T310 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 189C1A8615AA79B9	FASTA	392	44,409
	10 20 30 40 50 60 MEARVCVLFG AAALLRLLLL CVGVYQDQTL KLKYTDVDYH VFTDAARFIT QGESPYRRST 70 80 90 100 110 120 FRYTPLLALL LVPNVYLSLL FGKLLFGFCD LLSGLLMFRL LVLRGASHGS ACVSCGLWLL 130 140 150 160 170 180 NPLPMAVSTR GNAESVLAVL VLSTLLCLQL RKHTTAALLF GLSVHMKIYP VTYALPIALA 190 200 210 220 230 240 LTAAPARGRG VLLRFFSPAL LRFAAVSAAV FLSLGLIFYC RYGWEFLQEA YLYHLTRRDL 250 260 270 280 290 300 RHNFSPFFYL QYVCAERCWS SGLLPLLLLP QLLLLLLASA AFSSDLPFCC FLHTAVFVSF 310 320 330 340 350 360 NRVCTSQYFL WYLCLLPVVL PRLRLRLGRG LLLLLLWLLL QGLWLAPAYL LEFQGWNSFS 370 380 390 WIWAASLLFL LTNTFILAQI IQHYRPHDRK AD

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC053299 mRNA. Translation: AAH53299.1.
IPI	IPI00494149.
RefSeq	NP_956684.1. NM_200390.1.
UniGene	Dr.86984.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7T310.
Protein family/group databases
CAZy	GT50. Glycosyltransferase Family 50.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000032722; ENSDARP00000036819; ENSDARG00000024277.
GeneID	393361.
KEGG	dre:393361.
Organism-specific databases
ZFIN	ZDB-GENE-040426-1393. zgc:64177.
Phylogenomic databases
eggNOG	fiNOG10588.
GeneTree	ENSGT00390000017728.
HOVERGEN	HBG082137.
OrthoDB	EOG4Q2DG6.
PhylomeDB	Q7T310.
Gene expression databases
ArrayExpress	Q7T310.
Bgee	Q7T310.
Family and domain databases
InterPro	IPR007704. Mannosyltransferase_DXD. [Graphical view]
KO	K05284.
PANTHER	PTHR12886. Mannos_trans_DXD. 1 hit.
Pfam	PF05007. Mannosyl_trans. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIGM_DANRE

Accession

Primary (citable) accession number: Q7T310

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	October 1, 2003
Last modified:	November 16, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program