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Q7T2V3

- M3K10_XENLA

UniProt

Q7T2V3 - M3K10_XENLA

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Protein

Mitogen-activated protein kinase kinase kinase 10

Gene

map3k10

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Activates the JUN N-terminal pathway. Essential for pronephros and cement gland development.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.By similarity

Enzyme regulationi

Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei145 – 1451ATPPROSITE-ProRule annotation
Active sitei242 – 2421Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi124 – 1329ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. JUN kinase kinase kinase activity Source: UniProtKB
  3. kinase binding Source: UniProtKB
  4. protein homodimerization activity Source: UniProtKB
  5. protein tyrosine kinase activity Source: InterPro
  6. Rac GTPase binding Source: UniProtKB
  7. ubiquitin conjugating enzyme binding Source: UniProtKB

GO - Biological processi

  1. activation of JNKK activity Source: GOC
  2. cement gland development Source: UniProtKB
  3. positive regulation of JNK cascade Source: UniProtKB
  4. pronephros development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase 10 (EC:2.7.11.25)
Alternative name(s):
Mixed lineage kinase 2
Short name:
xMLK2
Gene namesi
Name:map3k10
Synonyms:mlk2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-920952. map3k10.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10051005Mitogen-activated protein kinase kinase kinase 10PRO_0000277827Add
BLAST

Post-translational modificationi

Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.By similarity
Mono- and poly-ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Expressioni

Tissue specificityi

In adults, strongly expressed in the brain and spleen with lower levels in pancreas, heart, muscle and kidney (at protein level). In the developing embryo, expressed at stage 22 in the cement gland. Weakly expressed in the pronephros from stage 24 or 25, with expression increasing in strength by stage 30 and continuing at least until stage 37. Expression in the developing pronephros correlates with epithelialization of the proximal pronephric tubules.1 Publication

Developmental stagei

Expressed zygotically from stages 12 to 14 (late gastrula to early neurula), increasing in concentration up to stages 40 to 45 (late tadpole). Expression continues through to adults.1 Publication

Interactioni

Subunit structurei

Homodimer. Binds to the GTPase rac1 but not cdc42 or rhoA. Interacts (via kinase domain) with pak1 (via kinase domain). Interacts with the ubiquitin-conjugating enzyme ube2d4.3 Publications

Protein-protein interaction databases

BioGridi99942. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ7T2V3.
SMRiQ7T2V3. Positions 33-91.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 9665SH3PROSITE-ProRule annotationAdd
BLAST
Domaini118 – 380263Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni404 – 42522Leucine-zipper 1Add
BLAST
Regioni439 – 46022Leucine-zipper 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi724 – 76542Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

HOVERGENiHBG067662.
KOiK04418.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR015785. MAP3K10.
IPR016231. MAPKKK9/10/11.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR001452. SH3_domain.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PANTHERiPTHR23257:SF370. PTHR23257:SF370. 1 hit.
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PIRSFiPIRSF000556. MAPKKK9_11. 1 hit.
PRINTSiPR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7T2V3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDGLPKDEAF LWQSSKDNKE NGVWSDVQSY GVSNPLWMAV FDYEPTAEEE
60 70 80 90 100
LTLRRGDLVE ILSKDSTVSG DEGWWTGKIK DKVGIFPSNY VVSDDKYTTL
110 120 130 140 150
TGAPKQCPLP LEIEFDELNL DEIIGVGGFG KVYKGLWRDE EVAVKAVRHD
160 170 180 190 200
PDEDINVTAE NVRQEAKIFC MLCHPNIIAL TGVCLKPPHL CLVMEYARGG
210 220 230 240 250
PLHRALAGKK VPAHVLVNWA VQIAKGMTYL HNEAIVPIIH RDLGSSNILI
260 270 280 290 300
LEKAENDDLF NKTLNITDFG LAREWQKTTK MSAAGTYAWM APEVIRLSLF
310 320 330 340 350
SKSSDVWSFG VLLWELLTGE VPYREIDALA VAYGVAMNKL TLPIPSTCPE
360 370 380 390 400
PFVRILEACW DPDPHSRPTF SCILEQLTTI EQSAMFQMPL ESFHSLQEDW
410 420 430 440 450
RLEIQQMFDE LRTKEKELRS REEELVRAAE EQRILEDLLK RREQELAERE
460 470 480 490 500
IDIVERELNI IMYQMYQEKP KVKKRKGNFK KSRLKLKDGN RISLPSGFEH
510 520 530 540 550
KITVQASPML DKCKGQGTSS YSPPGSPLII PRLRAIRLTP VDGSKTWGRS
560 570 580 590 600
SVLKKEEVTT SNKKKGRTWG PSSTQQKERV GGEERLKTLG EGNKQWSSSA
610 620 630 640 650
PNLGKSPKHT PISVGFASLT EMEEYADSDG SVPQSPYSQS YLTLPVQSDH
660 670 680 690 700
RSHPEDTAHA GAPSSDSPKR GSQSRRKSEL VLLGCASLLA AVALGSDLSE
710 720 730 740 750
LVPQEEKRKG IFQWAGRGPR RRASSPSRSM SYGEDSVIPS SSVTLISLSS
760 770 780 790 800
ISDCNSTRSL IRSDSDDIGL DHDNVSSGRG VKEDRGQQPN VGSNPLVDYK
810 820 830 840 850
VESFKRDPKQ SLTPTHVTVG RNNTTETRGH RRTPSDGAIR QVTQGHKRSP
860 870 880 890 900
SDGSTPYQCE PEPSPFPRLP DPHFVFPPPV RRKDTGVERP TSLEFAPRPR
910 920 930 940 950
PSSNRPRMDP WKFVSLSQTH SSSPSSGGGD ACSSGSAEGA QVADVEETLL
960 970 980 990 1000
DMEVEGQRLD STVPLCGLGL RPTTDPFFKY GNRRVLMKEL SISLLQYKVE

SGVLL
Length:1,005
Mass (Da):111,875
Last modified:October 1, 2003 - v1
Checksum:iB406102A77244309
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF510499 mRNA. Translation: AAP46399.1.
RefSeqiNP_001082629.1. NM_001089160.1.
UniGeneiXl.26500.

Genome annotation databases

GeneIDi398612.
KEGGixla:398612.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF510499 mRNA. Translation: AAP46399.1 .
RefSeqi NP_001082629.1. NM_001089160.1.
UniGenei Xl.26500.

3D structure databases

ProteinModelPortali Q7T2V3.
SMRi Q7T2V3. Positions 33-91.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 99942. 2 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 398612.
KEGGi xla:398612.

Organism-specific databases

CTDi 4294.
Xenbasei XB-GENE-920952. map3k10.

Phylogenomic databases

HOVERGENi HBG067662.
KOi K04418.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR015785. MAP3K10.
IPR016231. MAPKKK9/10/11.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR001452. SH3_domain.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view ]
PANTHERi PTHR23257:SF370. PTHR23257:SF370. 1 hit.
Pfami PF07714. Pkinase_Tyr. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view ]
PIRSFi PIRSF000556. MAPKKK9_11. 1 hit.
PRINTSi PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTi SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A tissue restricted role for the Xenopus Jun N-terminal kinase kinase kinase MLK2 in cement gland and pronephric tubule differentiation."
    Poitras L., Bisson N., Islam N., Moss T.
    Dev. Biol. 254:200-214(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RAC1, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Tail bud.
  2. "PAK interacts with NCK and MLK2 to regulate the activation of jun N-terminal kinase."
    Poitras L., Jean S., Islam N., Moss T.
    FEBS Lett. 543:129-135(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PAK1.
  3. "A ubiquitin-conjugating enzyme, ube2d3.2, regulates xMLK2 and pronephros formation in Xenopus."
    Jean S., Moss T.
    Differentiation 76:431-441(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBE2D4, HOMODIMERIZATION, UBIQUITINATION.

Entry informationi

Entry nameiM3K10_XENLA
AccessioniPrimary (citable) accession number: Q7T2V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 1, 2003
Last modified: October 1, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3