Reviewed, UniProtKB/Swiss-Prot Q7T2V3 (M3K10_XENLA)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase kinase kinase 10
    EC=2.7.11.25
Alternative name(s):
    Mixed lineage kinase 2

Gene names

Name:	map3k10
Synonyms:	mlk2

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	1005 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Activates the JUN N-terminal pathway By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation By similarity.
Subunit structure	Homodimer By similarity.
Post-translational modification	Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. Contains 1 SH3 domain.

Ontologies

Keywords
Domain	Repeat SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1005	1005	Mitogen-activated protein kinase kinase kinase 10		PRO_0000277827
Regions
	Domain	32 – 96	65	SH3
	Domain	118 – 380	263	Protein kinase
	Domain	404 – 425	22	Leucine-zipper 1 By similarity
	Domain	439 – 460	22	Leucine-zipper 2 By similarity
	Nucleotide binding	124 – 132	9	ATP By similarity
	Compositional bias	724 – 765	42	Ser-rich
Sites
	Active site	242	1	Proton acceptor By similarity
	Binding site	145	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7T2V3-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: B406102A77244309	FASTA	1,005	111,875
	10 20 30 40 50 60 MDGLPKDEAF LWQSSKDNKE NGVWSDVQSY GVSNPLWMAV FDYEPTAEEE LTLRRGDLVE 70 80 90 100 110 120 ILSKDSTVSG DEGWWTGKIK DKVGIFPSNY VVSDDKYTTL TGAPKQCPLP LEIEFDELNL 130 140 150 160 170 180 DEIIGVGGFG KVYKGLWRDE EVAVKAVRHD PDEDINVTAE NVRQEAKIFC MLCHPNIIAL 190 200 210 220 230 240 TGVCLKPPHL CLVMEYARGG PLHRALAGKK VPAHVLVNWA VQIAKGMTYL HNEAIVPIIH 250 260 270 280 290 300 RDLGSSNILI LEKAENDDLF NKTLNITDFG LAREWQKTTK MSAAGTYAWM APEVIRLSLF 310 320 330 340 350 360 SKSSDVWSFG VLLWELLTGE VPYREIDALA VAYGVAMNKL TLPIPSTCPE PFVRILEACW 370 380 390 400 410 420 DPDPHSRPTF SCILEQLTTI EQSAMFQMPL ESFHSLQEDW RLEIQQMFDE LRTKEKELRS 430 440 450 460 470 480 REEELVRAAE EQRILEDLLK RREQELAERE IDIVERELNI IMYQMYQEKP KVKKRKGNFK 490 500 510 520 530 540 KSRLKLKDGN RISLPSGFEH KITVQASPML DKCKGQGTSS YSPPGSPLII PRLRAIRLTP 550 560 570 580 590 600 VDGSKTWGRS SVLKKEEVTT SNKKKGRTWG PSSTQQKERV GGEERLKTLG EGNKQWSSSA 610 620 630 640 650 660 PNLGKSPKHT PISVGFASLT EMEEYADSDG SVPQSPYSQS YLTLPVQSDH RSHPEDTAHA 670 680 690 700 710 720 GAPSSDSPKR GSQSRRKSEL VLLGCASLLA AVALGSDLSE LVPQEEKRKG IFQWAGRGPR 730 740 750 760 770 780 RRASSPSRSM SYGEDSVIPS SSVTLISLSS ISDCNSTRSL IRSDSDDIGL DHDNVSSGRG 790 800 810 820 830 840 VKEDRGQQPN VGSNPLVDYK VESFKRDPKQ SLTPTHVTVG RNNTTETRGH RRTPSDGAIR 850 860 870 880 890 900 QVTQGHKRSP SDGSTPYQCE PEPSPFPRLP DPHFVFPPPV RRKDTGVERP TSLEFAPRPR 910 920 930 940 950 960 PSSNRPRMDP WKFVSLSQTH SSSPSSGGGD ACSSGSAEGA QVADVEETLL DMEVEGQRLD 970 980 990 1000 STVPLCGLGL RPTTDPFFKY GNRRVLMKEL SISLLQYKVE SGVLL

References

[1]	"PAK interacts with NCK and MLK2 to regulate the activation of jun N-terminal kinase." Poitras L., Jean S., Islam N., Moss T. FEBS Lett. 543:129-135(2003) [PubMed: 12753919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF510499 mRNA. Translation: AAP46399.1.
RefSeq	NP_001082629.1.
UniGene	Xl.26500
3D structure databases
HSSP	HSSP built from PDB template 1R1W based on UniProtKB P08581.
ModBase	Search...
Genome annotation databases
GeneID	398612.
KEGG	xla:398612.
Organism-specific databases
CTD	398612.
Xenbase	XB-FEAT-920943. map3k10.
Phylogenomic databases
HOVERGEN	Q7T2V3.
Enzyme and pathway databases
BRENDA	2.7.11.25. 648.
Family and domain databases
InterPro	IPR015785. MAPKKK-like. IPR016231. MAPKKK9/10/11. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR001452. SH3_domain. IPR020473. SH3_region. IPR001245. Tyr_pkinase. [Graphical view]
PANTHER	PTHR23257:SF87. MAPKKK-like. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PIRSF	PIRSF000556. MAPKKK9_11. 1 hit.
PRINTS	PR00452. SH3DOMAIN. PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. PD000066. SH3. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

M3K10_XENLA

Accession

Primary (citable) accession number: Q7T2V3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	October 1, 2003
Last modified:	October 13, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project