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Q7T2V3

- M3K10_XENLA

UniProt

Q7T2V3 - M3K10_XENLA

Protein

Mitogen-activated protein kinase kinase kinase 10

Gene

map3k10

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Activates the JUN N-terminal pathway. Essential for pronephros and cement gland development.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei145 – 1451ATPPROSITE-ProRule annotation
    Active sitei242 – 2421Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi124 – 1329ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. JUN kinase kinase kinase activity Source: UniProtKB
    3. kinase binding Source: UniProtKB
    4. protein homodimerization activity Source: UniProtKB
    5. protein tyrosine kinase activity Source: InterPro
    6. Rac GTPase binding Source: UniProtKB
    7. ubiquitin conjugating enzyme binding Source: UniProtKB

    GO - Biological processi

    1. activation of JNKK activity Source: GOC
    2. cement gland development Source: UniProtKB
    3. positive regulation of JNK cascade Source: UniProtKB
    4. pronephros development Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase kinase kinase 10 (EC:2.7.11.25)
    Alternative name(s):
    Mixed lineage kinase 2
    Short name:
    xMLK2
    Gene namesi
    Name:map3k10
    Synonyms:mlk2
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-920952. map3k10.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10051005Mitogen-activated protein kinase kinase kinase 10PRO_0000277827Add
    BLAST

    Post-translational modificationi

    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.By similarity
    Mono- and poly-ubiquitinated.1 Publication

    Keywords - PTMi

    Ubl conjugation

    Expressioni

    Tissue specificityi

    In adults, strongly expressed in the brain and spleen with lower levels in pancreas, heart, muscle and kidney (at protein level). In the developing embryo, expressed at stage 22 in the cement gland. Weakly expressed in the pronephros from stage 24 or 25, with expression increasing in strength by stage 30 and continuing at least until stage 37. Expression in the developing pronephros correlates with epithelialization of the proximal pronephric tubules.1 Publication

    Developmental stagei

    Expressed zygotically from stages 12 to 14 (late gastrula to early neurula), increasing in concentration up to stages 40 to 45 (late tadpole). Expression continues through to adults.1 Publication

    Interactioni

    Subunit structurei

    Homodimer. Binds to the GTPase rac1 but not cdc42 or rhoA. Interacts (via kinase domain) with pak1 (via kinase domain). Interacts with the ubiquitin-conjugating enzyme ube2d4.3 Publications

    Protein-protein interaction databases

    BioGridi99942. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7T2V3.
    SMRiQ7T2V3. Positions 33-91.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 9665SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini118 – 380263Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni404 – 42522Leucine-zipper 1Add
    BLAST
    Regioni439 – 46022Leucine-zipper 2Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi724 – 76542Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH3 domain

    Phylogenomic databases

    HOVERGENiHBG067662.
    KOiK04418.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR015785. MAP3K10.
    IPR016231. MAPKKK9/10/11.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR001452. SH3_domain.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PANTHERiPTHR23257:SF370. PTHR23257:SF370. 1 hit.
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000556. MAPKKK9_11. 1 hit.
    PRINTSiPR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7T2V3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDGLPKDEAF LWQSSKDNKE NGVWSDVQSY GVSNPLWMAV FDYEPTAEEE     50
    LTLRRGDLVE ILSKDSTVSG DEGWWTGKIK DKVGIFPSNY VVSDDKYTTL 100
    TGAPKQCPLP LEIEFDELNL DEIIGVGGFG KVYKGLWRDE EVAVKAVRHD 150
    PDEDINVTAE NVRQEAKIFC MLCHPNIIAL TGVCLKPPHL CLVMEYARGG 200
    PLHRALAGKK VPAHVLVNWA VQIAKGMTYL HNEAIVPIIH RDLGSSNILI 250
    LEKAENDDLF NKTLNITDFG LAREWQKTTK MSAAGTYAWM APEVIRLSLF 300
    SKSSDVWSFG VLLWELLTGE VPYREIDALA VAYGVAMNKL TLPIPSTCPE 350
    PFVRILEACW DPDPHSRPTF SCILEQLTTI EQSAMFQMPL ESFHSLQEDW 400
    RLEIQQMFDE LRTKEKELRS REEELVRAAE EQRILEDLLK RREQELAERE 450
    IDIVERELNI IMYQMYQEKP KVKKRKGNFK KSRLKLKDGN RISLPSGFEH 500
    KITVQASPML DKCKGQGTSS YSPPGSPLII PRLRAIRLTP VDGSKTWGRS 550
    SVLKKEEVTT SNKKKGRTWG PSSTQQKERV GGEERLKTLG EGNKQWSSSA 600
    PNLGKSPKHT PISVGFASLT EMEEYADSDG SVPQSPYSQS YLTLPVQSDH 650
    RSHPEDTAHA GAPSSDSPKR GSQSRRKSEL VLLGCASLLA AVALGSDLSE 700
    LVPQEEKRKG IFQWAGRGPR RRASSPSRSM SYGEDSVIPS SSVTLISLSS 750
    ISDCNSTRSL IRSDSDDIGL DHDNVSSGRG VKEDRGQQPN VGSNPLVDYK 800
    VESFKRDPKQ SLTPTHVTVG RNNTTETRGH RRTPSDGAIR QVTQGHKRSP 850
    SDGSTPYQCE PEPSPFPRLP DPHFVFPPPV RRKDTGVERP TSLEFAPRPR 900
    PSSNRPRMDP WKFVSLSQTH SSSPSSGGGD ACSSGSAEGA QVADVEETLL 950
    DMEVEGQRLD STVPLCGLGL RPTTDPFFKY GNRRVLMKEL SISLLQYKVE 1000
    SGVLL 1005
    Length:1,005
    Mass (Da):111,875
    Last modified:October 1, 2003 - v1
    Checksum:iB406102A77244309
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF510499 mRNA. Translation: AAP46399.1.
    RefSeqiNP_001082629.1. NM_001089160.1.
    UniGeneiXl.26500.

    Genome annotation databases

    GeneIDi398612.
    KEGGixla:398612.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF510499 mRNA. Translation: AAP46399.1 .
    RefSeqi NP_001082629.1. NM_001089160.1.
    UniGenei Xl.26500.

    3D structure databases

    ProteinModelPortali Q7T2V3.
    SMRi Q7T2V3. Positions 33-91.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 99942. 2 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 398612.
    KEGGi xla:398612.

    Organism-specific databases

    CTDi 4294.
    Xenbasei XB-GENE-920952. map3k10.

    Phylogenomic databases

    HOVERGENi HBG067662.
    KOi K04418.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR015785. MAP3K10.
    IPR016231. MAPKKK9/10/11.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR001452. SH3_domain.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    PANTHERi PTHR23257:SF370. PTHR23257:SF370. 1 hit.
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000556. MAPKKK9_11. 1 hit.
    PRINTSi PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A tissue restricted role for the Xenopus Jun N-terminal kinase kinase kinase MLK2 in cement gland and pronephric tubule differentiation."
      Poitras L., Bisson N., Islam N., Moss T.
      Dev. Biol. 254:200-214(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RAC1, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Tail bud.
    2. "PAK interacts with NCK and MLK2 to regulate the activation of jun N-terminal kinase."
      Poitras L., Jean S., Islam N., Moss T.
      FEBS Lett. 543:129-135(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PAK1.
    3. "A ubiquitin-conjugating enzyme, ube2d3.2, regulates xMLK2 and pronephros formation in Xenopus."
      Jean S., Moss T.
      Differentiation 76:431-441(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UBE2D4, HOMODIMERIZATION, UBIQUITINATION.

    Entry informationi

    Entry nameiM3K10_XENLA
    AccessioniPrimary (citable) accession number: Q7T2V3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3