Reviewed, UniProtKB/Swiss-Prot Q7T2Q5 (PA21_BUNFL)

Names and origin

Protein names	Recommended name:     Phospholipase A2-1     EC=3.1.1.4 Alternative name(s):     Phosphatidylcholine 2-acylhydrolase     PA2-I
Organism	Bungarus flaviceps flaviceps (Red-headed krait)
Taxonomic identifier	8615 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Bungarinae › Bungarus

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the phospholipase A2 family. Group I subfamily.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 27	8	By similarity		PRO_0000271457
	Chain	28 – 147	120	Phospholipase A2-1		PRO_5000050835
Sites
	Active site	75	1	By similarity
	Active site	121	1	By similarity
	Metal binding	55	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	57	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	59	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	76	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	38 ↔ 99		By similarity
	Disulfide bond	54 ↔ 146		By similarity
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	71 ↔ 127		By similarity
	Disulfide bond	78 ↔ 120		By similarity
	Disulfide bond	88 ↔ 113		By similarity
	Disulfide bond	106 ↔ 118		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7T2Q5-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: D1FFB08F58453225	FASTA	147	16,169
	10 20 30 40 50 60 MNPAHLLVLA AVCVSLLGAA IVPPQPLNLY QFNNMIQCAN HGNRPTWHYA HYGCYCGKGG 70 80 90 100 110 120 GGTAVDELDR CCQTHDNCYG EAEKLPKCSP YYKTYKYDCS EGKLTCKDAP GSCERSVCDC 130 140 DRVAANCFAG APYNNDNFFI SFKENCQ

References

[1]

"Molecular cloning of the major lethal toxins from two kraits (Bungarus flaviceps and Bungarus candidus)." Yanoshita R., Ogawa Y., Murayama N., Omori-Satoh T., Saguchi K., Higuchi S., Khow O., Chanhome L., Samejima Y., Sitprija V. Toxicon 47:416-424(2006) [PubMed: 16458338] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Venom gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB112359 mRNA. Translation: BAC77655.1.
3D structure databases
HSSP	HSSP built from PDB template 1FE5 based on UniProtKB Q9DF52.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q7T2Q5.
Enzyme and pathway databases
BRENDA	3.1.1.4. 322414.
Family and domain databases
InterPro	IPR016090. Phospholipase_A2. IPR013090. Phospholipase_A2_AS. IPR001211. Phospholipase_A2_euk. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
PANTHER	PTHR11716. Phospholipase_A2. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
ProDom	PD000303. PhospholipaseA2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00085. PA2c. 1 hit. [Graphical view]
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA21_BUNFL

Accession

Primary (citable) accession number: Q7T2Q5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)