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Protein

Cysteine-rich venom protein natrin-1

Gene
N/A
Organism
Naja atra (Chinese cobra)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits calcium-activated potassium channels (KCa1.1/KCNMA1), voltage-gated potassium channel Kv1.3/KCNA3, and the calcium release channel/ryanodine receptor (RyR). Binds specifically to type 1 RyR (RyR1) from skeletal muscle. Inhibit both the binding of ryanodine to RyR1, and RyR1's calcium-channel activity. Inhibits carbachol-induced muscle contraction and weakly blocks muscle contraction evoked by potassium.4 Publications

Keywords - Molecular functioni

Calcium channel impairing toxin, Calcium-activated potassium channel impairing toxin, Ion channel impairing toxin, Potassium channel impairing toxin, Ryanodine-sensitive calcium-release channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Protein family/group databases

TCDBi8.B.9.1.2. the triflin toxin (triflin or crisp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine-rich venom protein natrin-1
Alternative name(s):
Cysteine-rich venom protein 1
NA-CRVP1
Protein G2a
OrganismiNaja atra (Chinese cobra)
Taxonomic identifieri8656 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 183 PublicationsAdd BLAST18
ChainiPRO_000000627919 – 239Cysteine-rich venom protein natrin-1Add BLAST221

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi74 ↔ 152
Disulfide bondi91 ↔ 166
Disulfide bondi147 ↔ 163
Disulfide bondi185 ↔ 192
Disulfide bondi188 ↔ 197
Disulfide bondi201 ↔ 234
Disulfide bondi210 ↔ 228
Disulfide bondi219 ↔ 232

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1239
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 25Combined sources3
Helixi30 – 45Combined sources16
Beta strandi51 – 53Combined sources3
Helixi61 – 71Combined sources11
Turni72 – 74Combined sources3
Helixi81 – 83Combined sources3
Beta strandi92 – 100Combined sources9
Helixi104 – 112Combined sources9
Helixi113 – 117Combined sources5
Turni120 – 122Combined sources3
Beta strandi123 – 126Combined sources4
Helixi132 – 137Combined sources6
Beta strandi144 – 151Combined sources8
Beta strandi155 – 167Combined sources13
Turni172 – 176Combined sources5
Beta strandi181 – 183Combined sources3
Turni184 – 187Combined sources4
Beta strandi191 – 193Combined sources3
Beta strandi206 – 208Combined sources3
Helixi210 – 217Combined sources8
Helixi218 – 220Combined sources3
Helixi222 – 227Combined sources6
Helixi229 – 233Combined sources5
Beta strandi236 – 238Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XTAX-ray1.58A/B19-239[»]
1XX5X-ray2.40A/B/C19-239[»]
2GIZX-ray1.68A/B19-239[»]
3MZ8X-ray2.70A/B19-239[»]
ProteinModelPortaliQ7T1K6.
SMRiQ7T1K6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7T1K6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 165SCPAdd BLAST129
Domaini201 – 234ShKTPROSITE-ProRule annotationAdd BLAST34

Sequence similaritiesi

Belongs to the CRISP family.Curated
Contains 1 SCP domain.Curated
Contains 1 ShKT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG004184.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
IPR003582. ShKT_dom.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]
PRINTSiPR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
PS51670. SHKT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7T1K6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAFSLLCFA AVLQQSFGNV DFNSESTRRK KKQKEIVDLH NSLRRRVSPT
60 70 80 90 100
ASNMLKMEWY PEAASNAERW ANTCSLNHSP DNLRVLEGIQ CGESIYMSSN
110 120 130 140 150
ARTWTEIIHL WHDEYKNFVY GVGANPPGSV TGHYTQIVWY QTYRAGCAVS
160 170 180 190 200
YCPSSAWSYF YVCQYCPSGN FQGKTATPYK LGPPCGDCPS ACDNGLCTNP
210 220 230
CTIYNKLTNC DSLLKQSSCQ DDWIKSNCPA SCFCRNKII
Length:239
Mass (Da):26,882
Last modified:October 1, 2003 - v1
Checksum:i70F34EED0391BF26
GO

Mass spectrometryi

Molecular mass is 24937 Da from positions 19 - 239. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY324325 mRNA. Translation: AAP85301.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY324325 mRNA. Translation: AAP85301.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XTAX-ray1.58A/B19-239[»]
1XX5X-ray2.40A/B/C19-239[»]
2GIZX-ray1.68A/B19-239[»]
3MZ8X-ray2.70A/B19-239[»]
ProteinModelPortaliQ7T1K6.
SMRiQ7T1K6.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi8.B.9.1.2. the triflin toxin (triflin or crisp) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG004184.

Miscellaneous databases

EvolutionaryTraceiQ7T1K6.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
IPR003582. ShKT_dom.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]
PRINTSiPR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
PS51670. SHKT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRVP1_NAJAT
AccessioniPrimary (citable) accession number: Q7T1K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: October 1, 2003
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.