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Protein

Cysteine-rich venom protein natrin-1

Gene
N/A
Organism
Naja atra (Chinese cobra)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits calcium-activated potassium channels (KCa1.1/KCNMA1), voltage-gated potassium channel Kv1.3/KCNA3, and the calcium release channel/ryanodine receptor (RyR). Binds specifically to type 1 RyR (RyR1) from skeletal muscle. Inhibit both the binding of ryanodine to RyR1, and RyR1's calcium-channel activity. Inhibits carbachol-induced muscle contraction and weakly blocks muscle contraction evoked by potassium.4 Publications

Keywords - Molecular functioni

Calcium channel impairing toxin, Calcium-activated potassium channel impairing toxin, Ion channel impairing toxin, Potassium channel impairing toxin, Ryanodine-sensitive calcium-release channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Protein family/group databases

TCDBi8.B.9.1.2. the triflin toxin (triflin or crisp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine-rich venom protein natrin-1
Alternative name(s):
Cysteine-rich venom protein 1
NA-CRVP1
Protein G2a
OrganismiNaja atra (Chinese cobra)
Taxonomic identifieri8656 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18183 PublicationsAdd
BLAST
Chaini19 – 239221Cysteine-rich venom protein natrin-1PRO_0000006279Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi74 ↔ 152
Disulfide bondi91 ↔ 166
Disulfide bondi147 ↔ 163
Disulfide bondi185 ↔ 192
Disulfide bondi188 ↔ 197
Disulfide bondi201 ↔ 234
Disulfide bondi210 ↔ 228
Disulfide bondi219 ↔ 232

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
239
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi23 – 253Combined sources
Helixi30 – 4516Combined sources
Beta strandi51 – 533Combined sources
Helixi61 – 7111Combined sources
Turni72 – 743Combined sources
Helixi81 – 833Combined sources
Beta strandi92 – 1009Combined sources
Helixi104 – 1129Combined sources
Helixi113 – 1175Combined sources
Turni120 – 1223Combined sources
Beta strandi123 – 1264Combined sources
Helixi132 – 1376Combined sources
Beta strandi144 – 1518Combined sources
Beta strandi155 – 16713Combined sources
Turni172 – 1765Combined sources
Beta strandi181 – 1833Combined sources
Turni184 – 1874Combined sources
Beta strandi191 – 1933Combined sources
Beta strandi206 – 2083Combined sources
Helixi210 – 2178Combined sources
Helixi218 – 2203Combined sources
Helixi222 – 2276Combined sources
Helixi229 – 2335Combined sources
Beta strandi236 – 2383Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XTAX-ray1.58A/B19-239[»]
1XX5X-ray2.40A/B/C19-239[»]
2GIZX-ray1.68A/B19-239[»]
3MZ8X-ray2.70A/B19-239[»]
ProteinModelPortaliQ7T1K6.
SMRiQ7T1K6. Positions 24-239.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7T1K6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 165129SCPAdd
BLAST
Domaini201 – 23434ShKTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CRISP family.Curated
Contains 1 SCP domain.Curated
Contains 1 ShKT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG004184.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
IPR003582. ShKT_dom.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]
PRINTSiPR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
PS51670. SHKT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7T1K6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAFSLLCFA AVLQQSFGNV DFNSESTRRK KKQKEIVDLH NSLRRRVSPT
60 70 80 90 100
ASNMLKMEWY PEAASNAERW ANTCSLNHSP DNLRVLEGIQ CGESIYMSSN
110 120 130 140 150
ARTWTEIIHL WHDEYKNFVY GVGANPPGSV TGHYTQIVWY QTYRAGCAVS
160 170 180 190 200
YCPSSAWSYF YVCQYCPSGN FQGKTATPYK LGPPCGDCPS ACDNGLCTNP
210 220 230
CTIYNKLTNC DSLLKQSSCQ DDWIKSNCPA SCFCRNKII
Length:239
Mass (Da):26,882
Last modified:October 1, 2003 - v1
Checksum:i70F34EED0391BF26
GO

Mass spectrometryi

Molecular mass is 24937 Da from positions 19 - 239. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY324325 mRNA. Translation: AAP85301.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY324325 mRNA. Translation: AAP85301.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XTAX-ray1.58A/B19-239[»]
1XX5X-ray2.40A/B/C19-239[»]
2GIZX-ray1.68A/B19-239[»]
3MZ8X-ray2.70A/B19-239[»]
ProteinModelPortaliQ7T1K6.
SMRiQ7T1K6. Positions 24-239.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi8.B.9.1.2. the triflin toxin (triflin or crisp) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG004184.

Miscellaneous databases

EvolutionaryTraceiQ7T1K6.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
IPR003582. ShKT_dom.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]
PRINTSiPR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
PS51670. SHKT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Purification and cloning of toxins of CRISP family from Naja atra venoms."
    Wang J., Teng M., Niu L.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Purification and cloning of cysteine-rich proteins from Trimeresurus jerdonii and Naja atra venoms."
    Jin Y., Lu Q., Zhou X., Zhu S., Li R., Wang W., Xiong Y.
    Toxicon 42:539-547(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 19-60.
    Tissue: Venom.
  3. "Purification and characterization of Taiwan cobra venom proteins with weak toxicity."
    Chang L.-S., Liou J.-C., Lin S.-R., Cheng Y.-C.
    Toxicon 45:21-25(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 19-43, FUNCTION, MASS SPECTROMETRY.
    Tissue: Venom.
  4. "Structural and functional analysis of natrin, a venom protein that targets various ion channels."
    Wang F., Li H., Liu M.N., Song H., Han H.-M., Wang Q.-L., Yin C.-C., Zhou Y.-C., Qi Z., Shu Y.-Y., Lin Z.-J., Jiang T.
    Biochem. Biophys. Res. Commun. 351:443-448(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 19-38, X-RAY CRYSTALLOGRAPHY (1.68 ANGSTROMS) OF 19-239, FUNCTION, DISULFIDE BONDS.
    Tissue: Venom.
  5. "Structural and functional characterization of ryanodine receptor-natrin toxin interaction."
    Zhou Q., Wang Q.-L., Meng X., Strauss J., Shu Y., Jiang T., Wagenknecht T., Yin C.-C., Sui S.-F., Liu Z.
    Biophys. J. 95:4289-4299(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Purification, crystallization and preliminary X-ray crystallographic analysis of a cysteine-rich secretory protein (CRISP) from Naja atra venom."
    Wang Y.-L., Goh K.X., Wu W.G., Chen C.J.
    Acta Crystallogr. D 60:1912-1915(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.58 ANGSTROMS) OF 19-239, DISULFIDE BONDS.
  7. "Blocking effect and crystal structure of natrin toxin, a cysteine-rich secretory protein from Naja atra venom that targets the BKCa channel."
    Wang J., Shen B., Guo M., Lou X., Duan Y., Cheng X.P., Teng M., Niu L., Liu Q., Huang Q., Hao Q.
    Biochemistry 44:10145-10152(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 19-239, DISULFIDE BONDS, FUNCTION.
    Tissue: Venom.

Entry informationi

Entry nameiCRVP1_NAJAT
AccessioniPrimary (citable) accession number: Q7T1K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: October 1, 2003
Last modified: May 11, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.