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Protein

Dead end protein 1

Gene

dnd

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding factor that positively regulates gene expression by prohibiting miRNA-mediated gene suppression. Relieves miRNA repression in germline cells. Prohibits the function of several miRNAs by blocking the accessibility of target mRNAs (By similarity). Sequence-specific RNA-binding factor that binds to U-rich regions (URRs) in the 3'untranslated region (3'-UTR) of several mRNAs (By similarity). Does not bind to miRNAs (By similarity). Germline-specific protein required for the primordial germ cell (PGC) survival and migration during early embryonic development.By similarity2 Publications

GO - Molecular functioni

  • AU-rich element binding Source: UniProtKB
  • mRNA 3'-UTR binding Source: ZFIN
  • mRNA binding Source: ZFIN
  • nucleotide binding Source: InterPro
  • RNA binding Source: ZFIN

GO - Biological processi

  • germ cell development Source: ZFIN
  • germ cell migration Source: ZFIN
  • heart contraction Source: ZFIN
  • mRNA stabilization Source: ZFIN
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of gene silencing by miRNA Source: UniProtKB
  • positive regulation of gene expression Source: ZFIN
  • regulation of cell motility Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dead end protein 1
Gene namesi
Name:dnd
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 14

Organism-specific databases

ZFINiZDB-GENE-030828-4. dnd1.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Perinuclear germ granules, also called germ plasm or chromatoid body.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • germ cell nucleus Source: ZFIN
  • nucleus Source: UniProtKB
  • P granule Source: ZFIN
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi104 – 1041Y → C: Inhibits RNA-binding and the relieve of miRNA repression activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Dead end protein 1PRO_0000081570Add
BLAST

Proteomic databases

PaxDbiQ7T1H5.
PRIDEiQ7T1H5.

Expressioni

Developmental stagei

Expressed in the germ plasm and the primordial germ cells (PGCs). At the onset of embryogenesis, maternal product is located in granules distributed throughout the cortex of the one-cell stage embryo. At 0.5 hour post fertilization (hpf) is expressed at the vegetal part of the blastomere. At 1 hpf expressed at the distal parts of the first two cleavage furrows. At 4 hpf expressed exclusively in PGCs. Expressed in PGCs during their migration and at their arrival in the presumptive gonade. At 5 days post-fertilization (dpf) still expressed in PGCs, albeit at a lower level.1 Publication

Gene expression databases

BgeeiQ7T1H5.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000035017.

Structurei

3D structure databases

ProteinModelPortaliQ7T1H5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 13879RRMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IUE0. Eukaryota.
ENOG410YAVC. LUCA.
GeneTreeiENSGT00550000074366.
HOGENOMiHOG000112240.
HOVERGENiHBG051370.
InParanoidiQ7T1H5.
OMAiVGPLWEF.
OrthoDBiEOG73JKW2.
PhylomeDBiQ7T1H5.
TreeFamiTF314932.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7T1H5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGDMDAQQQ ELQQILNPQK LKSLQEWMQR NSITLTQVNG QRKYGGPPPG
60 70 80 90 100
WQGPAPGSGC EVFISQIPND VYEDRLIPLF QSIGTIYEFR LMMNFSGQTR
110 120 130 140 150
GFAYAKYGDP LTASAAVTTL HQYRLPEGGC LTVRRSTEKR QLRLGDLPVS
160 170 180 190 200
MNESKLLMVL QMLSDGVEDV LLKPPGPKGK EVVALVNYTS HYAASMAKKV
210 220 230 240 250
LVEAFRNRYG ISITVRWTSF SKSKRVEDTP QEDSCVTPLV LKPLSKPSLL
260 270 280 290 300
HYDVPAHQSL LPLFRAVGGP TTSEQRDEMI PQPTIMSRNE LIPQSSIRQR
310 320 330 340 350
DEMVPQLPIR PRDGMAPQSP ISLDAVSHLQ WMCEVNRLGS PQYEVHFHHA
360 370 380 390 400
APDGFLYFAF KVLIPGLPLP LYGFVQILPG TSARAMKSEV YRAAAEQVIQ
410
TLCRVSNLRP F
Length:411
Mass (Da):46,020
Last modified:October 1, 2003 - v1
Checksum:i36CE5A3F12DFFFD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY225448 mRNA. Translation: AAP22373.1.
BC095578 mRNA. Translation: AAH95578.1.
RefSeqiNP_997960.1. NM_212795.1.
UniGeneiDr.26907.

Genome annotation databases

EnsembliENSDART00000036463; ENSDARP00000035017; ENSDARG00000022813.
GeneIDi373074.
KEGGidre:373074.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY225448 mRNA. Translation: AAP22373.1.
BC095578 mRNA. Translation: AAH95578.1.
RefSeqiNP_997960.1. NM_212795.1.
UniGeneiDr.26907.

3D structure databases

ProteinModelPortaliQ7T1H5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000035017.

Proteomic databases

PaxDbiQ7T1H5.
PRIDEiQ7T1H5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000036463; ENSDARP00000035017; ENSDARG00000022813.
GeneIDi373074.
KEGGidre:373074.

Organism-specific databases

CTDi373863.
ZFINiZDB-GENE-030828-4. dnd1.

Phylogenomic databases

eggNOGiENOG410IUE0. Eukaryota.
ENOG410YAVC. LUCA.
GeneTreeiENSGT00550000074366.
HOGENOMiHOG000112240.
HOVERGENiHBG051370.
InParanoidiQ7T1H5.
OMAiVGPLWEF.
OrthoDBiEOG73JKW2.
PhylomeDBiQ7T1H5.
TreeFamiTF314932.

Miscellaneous databases

NextBioi20813348.
PROiQ7T1H5.

Gene expression databases

BgeeiQ7T1H5.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Dead end, a novel vertebrate germ plasm component, is required for zebrafish primordial germ cell migration and survival."
    Weidinger G., Stebler J., Slanchev K., Dumstrei K., Wise C., Lovell-Badge R., Thisse C., Thisse B., Raz E.
    Curr. Biol. 13:1429-1434(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN PGC MIGRATION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  3. Cited for: FUNCTION, MUTAGENESIS OF TYR-104, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDND1_DANRE
AccessioniPrimary (citable) accession number: Q7T1H5
Secondary accession number(s): Q4VBK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 1, 2003
Last modified: May 11, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.