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Q7T103 (P5F11_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 5, transcription factor 1.1
Alternative name(s):
POU class V protein oct-25
Short name=XOct-25
Gene names
Name:pou5f1.1
Synonyms:oct-25
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Activates transcription when directly bound to the octamer DNA sequence, but can form repression complexes with other proteins at the promoter site to inhibit transcription. Binds to the promoter of the vent2-B gene to activate transcription when in the presence of other BMP signaling factors also bound to the promoter. Inhibits the competence of ectodermal cells to respond to BMP during embryogenesis thereby inhibiting epidermal differentiation and promoting neural induction. Antagonizes the activity of nodal/activin signaling by forming a transcriptional repression complex on the gsc and mix2 gene promoters to inhibit their transcription, and thus maintain the undifferentiated state of embryonic cells to prevent them from differentiating prematurely. Acts maternally to inhibit vegt and beta-catenin-activated gene transcription by forming a transcriptional repression complex on the nodal/nr1 and siamois promoters to inhibit their transcription. Ref.1 Ref.2 Ref.3 Ref.5 Ref.6 Ref.7

Subunit structure

Interacts with components of the transcription complex that assembles on the vent2-B gene, including vent2 (via C-terminus), smad1 and smad4. Forms a repression complex on the promoters of the gsc and mix2 genes via interactions with the nodal/activin signaling pathway transducers foxh1/fast1, gtf2ird1/wbscr11 and smad2. Forms a repression complex on the promoters of the nodal/nr1 and siamois genes with the maternal factors tcf7l1/tcf3 and vegt. Ref.2 Ref.6 Ref.7

Subcellular location

Nucleus By similarity UniProtKB Q01860.

Tissue specificity

Highly enriched within the animal half of developing embryos within ectodermal and mesodermal regions. Expressed in the neuroectoderm at the early neurula stage, with expression initially extending to the future hindbrain/midbrain boundary, but later shifting toward the posterior pole where it persists within the tip of the tail in hatching embryos. Expressed at very low levels in the adult kidney. Ref.1 Ref.2 Ref.6

Developmental stage

Expressed both maternally and zygotically. Expressed in oocytes and cleavage stage embryos. Expression increases during blastula stages, reaches a maximum at stage 11 during gastrulation, then subsequently declines to be no longer detectable by the end of neurulation. Ref.1 Ref.2

Sequence similarities

Belongs to the POU transcription factor family. Class-5 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Ontologies

Keywords
   Biological processDifferentiation
Transcription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
Gene Ontology (GO)
   Biological_processgastrulation

Inferred from mutant phenotype Ref.5. Source: UniProtKB

negative regulation of BMP signaling pathway

Inferred from mutant phenotype Ref.3. Source: UniProtKB

negative regulation of activin receptor signaling pathway

Inferred from mutant phenotype Ref.5Ref.7. Source: UniProtKB

negative regulation of epidermal cell differentiation

Inferred from mutant phenotype Ref.3. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from mutant phenotype Ref.6Ref.7. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from direct assay Ref.2Ref.3Ref.7. Source: UniProtKB

regulation of neurogenesis

Inferred from mutant phenotype Ref.3. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred by curator Ref.2. Source: UniProtKB

transcription factor complex

Inferred from physical interaction Ref.2Ref.6Ref.7. Source: UniProtKB

   Molecular_functionSMAD binding

Inferred from direct assay Ref.2Ref.7. Source: UniProtKB

sequence-specific DNA binding

Inferred from direct assay Ref.1. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

transcription regulatory region DNA binding

Inferred from direct assay Ref.2Ref.6Ref.7. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

tcf7l1-bQ90ZB63EBI-7438970,EBI-7439087
vegt-aP873773EBI-7438970,EBI-7439000

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 449449POU domain, class 5, transcription factor 1.1
PRO_0000390495

Regions

Domain227 – 30175POU-specific
DNA binding321 – 38060Homeobox

Experimental info

Sequence conflict291T → A in AAA49996. Ref.1
Sequence conflict711S → C in AAA49996. Ref.1
Sequence conflict1861T → A in ABH07383. Ref.3
Sequence conflict400 – 4012SP → R in AAA49996. Ref.1
Sequence conflict4281F → G in AAA49996. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q7T103 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 38430BD13CF4115F

FASTA44949,676
        10         20         30         40         50         60 
MYSQQPFPAF AFNAGLMQDP ANCHFGGYTG LGHPQPFSFA FSTLKSENGE SGVQGMGDCT 

        70         80         90        100        110        120 
TPVMPWNSLA SFDHQVQMEN NQQGNPPRAP SPTLSDSRIK VKEEVVHETD SGEESPEPKY 

       130        140        150        160        170        180 
PSPPNPSLYY PNAWTGAPFW QVNPTPGNNI NPMPNQTLVK NTSLPGNTTY PTPANQSPNT 

       190        200        210        220        230        240 
PVDCVTSSME SSRCSSTNSP NGAINERATT IPNGEMLDGG QSSDNEEEVP SESEMEQFAK 

       250        260        270        280        290        300 
DLKHKRVSLG YTQADVGYAL GVLYGKMFSQ TTICRFESLQ LSFKNMCQLK PFLERWVVEA 

       310        320        330        340        350        360 
ENNDNLQELI NREQVIAQTR KRKRRTNIEN IVKGTLESYF MKCPKPGAQE MVQIAKELNM 

       370        380        390        400        410        420 
DKDVVRVWFC NRRQKGKRQG MPTVEENDGE GYDVAQTMGS PPVGHYALQQ VVTPQGYMAA 

       430        440 
PQIYASAFHK NDLFPQTVPH GMAMGGHIG 

« Hide

References

« Hide 'large scale' references
[1]"Sequential expression of multiple POU proteins during amphibian early development."
Hinkley C.S., Martin J.F., Leibham D., Perry M.
Mol. Cell. Biol. 12:638-649(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Gastrula.
[2]"The POU factor Oct-25 regulates the Xvent-2B gene and counteracts terminal differentiation in Xenopus embryos."
Cao Y., Knochel S., Donow C., Miethe J., Kaufmann E., Knochel W.
J. Biol. Chem. 279:43735-43743(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH VENT2; SMAD1 AND SMAD4, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Gastrula.
[3]"The Xenopus POU class V transcription factor XOct-25 inhibits ectodermal competence to respond to bone morphogenetic protein-mediated embryonic induction."
Takebayashi-Suzuki K., Arita N., Murasaki E., Suzuki A.
Mech. Dev. 124:840-855(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Tissue: Gastrula.
[4]NIH - Xenopus Gene Collection (XGC) project
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Gastrula.
[5]"Xenopus POU factors of subclass V inhibit activin/nodal signaling during gastrulation."
Cao Y., Siegel D., Knochel W.
Mech. Dev. 123:614-625(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos."
Cao Y., Siegel D., Donow C., Knochel S., Yuan L., Knochel W.
EMBO J. 26:2942-2954(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TCF7L1 AND VEGT, TISSUE SPECIFICITY.
[7]"Oct25 represses transcription of nodal/activin target genes by interaction with signal transducers during Xenopus gastrulation."
Cao Y., Siegel D., Oswald F., Knochel W.
J. Biol. Chem. 283:34168-34177(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH FOXH1; GTF2IRD1 AND SMAD2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60074 mRNA. Translation: AAA49996.1.
AJ699165 mRNA. Translation: CAG27841.1.
DQ825761 mRNA. Translation: ABH07383.1.
BC055964 mRNA. Translation: AAH55964.1.
PIRB42022.
RefSeqNP_001079832.1. NM_001086363.1.
UniGeneXl.4957.

3D structure databases

ProteinModelPortalQ7T103.
SMRQ7T103. Positions 230-379.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ7T103. 2 interactions.
MINTMINT-4979275.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID379522.
KEGGxla:379522.

Organism-specific databases

CTD379522.
XenbaseXB-GENE-919851. pou5f3.2.

Phylogenomic databases

HOVERGENHBG053782.
KOK09367.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP5F11_XENLA
AccessionPrimary (citable) accession number: Q7T103
Secondary accession number(s): A9CDQ2, Q99293
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: October 1, 2003
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families