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Q7T103

- P5F11_XENLA

UniProt

Q7T103 - P5F11_XENLA

Protein

POU domain, class 5, transcription factor 1.1

Gene

pou5f1.1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Activates transcription when directly bound to the octamer DNA sequence, but can form repression complexes with other proteins at the promoter site to inhibit transcription. Binds to the promoter of the vent2-B gene to activate transcription when in the presence of other BMP signaling factors also bound to the promoter. Inhibits the competence of ectodermal cells to respond to BMP during embryogenesis thereby inhibiting epidermal differentiation and promoting neural induction. Antagonizes the activity of nodal/activin signaling by forming a transcriptional repression complex on the gsc and mix2 gene promoters to inhibit their transcription, and thus maintain the undifferentiated state of embryonic cells to prevent them from differentiating prematurely. Acts maternally to inhibit vegt and beta-catenin-activated gene transcription by forming a transcriptional repression complex on the nodal/nr1 and siamois promoters to inhibit their transcription.6 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi321 – 38060HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. sequence-specific DNA binding Source: UniProtKB
    3. sequence-specific DNA binding transcription factor activity Source: InterPro
    4. SMAD binding Source: UniProtKB
    5. transcription factor binding Source: UniProtKB
    6. transcription regulatory region DNA binding Source: UniProtKB

    GO - Biological processi

    1. gastrulation Source: UniProtKB
    2. negative regulation of activin receptor signaling pathway Source: UniProtKB
    3. negative regulation of BMP signaling pathway Source: UniProtKB
    4. negative regulation of epidermal cell differentiation Source: UniProtKB
    5. negative regulation of transcription, DNA-templated Source: UniProtKB
    6. positive regulation of transcription, DNA-templated Source: UniProtKB
    7. regulation of neurogenesis Source: UniProtKB
    8. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Differentiation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain, class 5, transcription factor 1.1
    Alternative name(s):
    POU class V protein oct-25Imported
    Short name:
    XOct-25Imported
    Gene namesi
    Name:pou5f1.1
    Synonyms:oct-25Imported
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-919851. pou5f3.2.

    Subcellular locationi

    Nucleus By similarityPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB
    2. transcription factor complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 449449POU domain, class 5, transcription factor 1.1PRO_0000390495Add
    BLAST

    Expressioni

    Tissue specificityi

    Highly enriched within the animal half of developing embryos within ectodermal and mesodermal regions. Expressed in the neuroectoderm at the early neurula stage, with expression initially extending to the future hindbrain/midbrain boundary, but later shifting toward the posterior pole where it persists within the tip of the tail in hatching embryos. Expressed at very low levels in the adult kidney.3 Publications

    Developmental stagei

    Expressed both maternally and zygotically. Expressed in oocytes and cleavage stage embryos. Expression increases during blastula stages, reaches a maximum at stage 11 during gastrulation, then subsequently declines to be no longer detectable by the end of neurulation.2 Publications

    Interactioni

    Subunit structurei

    Interacts with components of the transcription complex that assembles on the vent2-B gene, including vent2 (via C-terminus), smad1 and smad4. Forms a repression complex on the promoters of the gsc and mix2 genes via interactions with the nodal/activin signaling pathway transducers foxh1/fast1, gtf2ird1/wbscr11 and smad2. Forms a repression complex on the promoters of the nodal/nr1 and siamois genes with the maternal factors tcf7l1/tcf3 and vegt.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    tcf7l1-bQ90ZB63EBI-7438970,EBI-7439087
    vegt-aP873773EBI-7438970,EBI-7439000

    Protein-protein interaction databases

    IntActiQ7T103. 2 interactions.
    MINTiMINT-4979275.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7T103.
    SMRiQ7T103. Positions 230-379.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini227 – 30175POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the POU transcription factor family. Class-5 subfamily.Sequence Analysis
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    HOVERGENiHBG053782.
    KOiK09367.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7T103-1 [UniParc]FASTAAdd to Basket

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    MYSQQPFPAF AFNAGLMQDP ANCHFGGYTG LGHPQPFSFA FSTLKSENGE    50
    SGVQGMGDCT TPVMPWNSLA SFDHQVQMEN NQQGNPPRAP SPTLSDSRIK 100
    VKEEVVHETD SGEESPEPKY PSPPNPSLYY PNAWTGAPFW QVNPTPGNNI 150
    NPMPNQTLVK NTSLPGNTTY PTPANQSPNT PVDCVTSSME SSRCSSTNSP 200
    NGAINERATT IPNGEMLDGG QSSDNEEEVP SESEMEQFAK DLKHKRVSLG 250
    YTQADVGYAL GVLYGKMFSQ TTICRFESLQ LSFKNMCQLK PFLERWVVEA 300
    ENNDNLQELI NREQVIAQTR KRKRRTNIEN IVKGTLESYF MKCPKPGAQE 350
    MVQIAKELNM DKDVVRVWFC NRRQKGKRQG MPTVEENDGE GYDVAQTMGS 400
    PPVGHYALQQ VVTPQGYMAA PQIYASAFHK NDLFPQTVPH GMAMGGHIG 449
    Length:449
    Mass (Da):49,676
    Last modified:October 1, 2003 - v1
    Checksum:i38430BD13CF4115F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti29 – 291T → A in AAA49996. (PubMed:1732736)Curated
    Sequence conflicti71 – 711S → C in AAA49996. (PubMed:1732736)Curated
    Sequence conflicti186 – 1861T → A in ABH07383. (PubMed:17950579)Curated
    Sequence conflicti400 – 4012SP → R in AAA49996. (PubMed:1732736)Curated
    Sequence conflicti428 – 4281F → G in AAA49996. (PubMed:1732736)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60074 mRNA. Translation: AAA49996.1.
    AJ699165 mRNA. Translation: CAG27841.1.
    DQ825761 mRNA. Translation: ABH07383.1.
    BC055964 mRNA. Translation: AAH55964.1.
    PIRiB42022.
    RefSeqiNP_001079832.1. NM_001086363.1.
    UniGeneiXl.4957.

    Genome annotation databases

    GeneIDi379522.
    KEGGixla:379522.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60074 mRNA. Translation: AAA49996.1 .
    AJ699165 mRNA. Translation: CAG27841.1 .
    DQ825761 mRNA. Translation: ABH07383.1 .
    BC055964 mRNA. Translation: AAH55964.1 .
    PIRi B42022.
    RefSeqi NP_001079832.1. NM_001086363.1.
    UniGenei Xl.4957.

    3D structure databases

    ProteinModelPortali Q7T103.
    SMRi Q7T103. Positions 230-379.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q7T103. 2 interactions.
    MINTi MINT-4979275.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 379522.
    KEGGi xla:379522.

    Organism-specific databases

    CTDi 379522.
    Xenbasei XB-GENE-919851. pou5f3.2.

    Phylogenomic databases

    HOVERGENi HBG053782.
    KOi K09367.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequential expression of multiple POU proteins during amphibian early development."
      Hinkley C.S., Martin J.F., Leibham D., Perry M.
      Mol. Cell. Biol. 12:638-649(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Gastrula1 Publication.
    2. "The POU factor Oct-25 regulates the Xvent-2B gene and counteracts terminal differentiation in Xenopus embryos."
      Cao Y., Knochel S., Donow C., Miethe J., Kaufmann E., Knochel W.
      J. Biol. Chem. 279:43735-43743(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH VENT2; SMAD1 AND SMAD4, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Gastrula1 Publication.
    3. "The Xenopus POU class V transcription factor XOct-25 inhibits ectodermal competence to respond to bone morphogenetic protein-mediated embryonic induction."
      Takebayashi-Suzuki K., Arita N., Murasaki E., Suzuki A.
      Mech. Dev. 124:840-855(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Tissue: Gastrula1 Publication.
    4. NIH - Xenopus Gene Collection (XGC) project
      Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: GastrulaImported.
    5. "Xenopus POU factors of subclass V inhibit activin/nodal signaling during gastrulation."
      Cao Y., Siegel D., Knochel W.
      Mech. Dev. 123:614-625(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos."
      Cao Y., Siegel D., Donow C., Knochel S., Yuan L., Knochel W.
      EMBO J. 26:2942-2954(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TCF7L1 AND VEGT, TISSUE SPECIFICITY.
    7. "Oct25 represses transcription of nodal/activin target genes by interaction with signal transducers during Xenopus gastrulation."
      Cao Y., Siegel D., Oswald F., Knochel W.
      J. Biol. Chem. 283:34168-34177(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH FOXH1; GTF2IRD1 AND SMAD2.

    Entry informationi

    Entry nameiP5F11_XENLA
    AccessioniPrimary (citable) accession number: Q7T103
    Secondary accession number(s): A9CDQ2, Q99293
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3