Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q7T103

- P5F11_XENLA

UniProt

Q7T103 - P5F11_XENLA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

POU domain, class 5, transcription factor 1.1

Gene

pou5f1.1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Activates transcription when directly bound to the octamer DNA sequence, but can form repression complexes with other proteins at the promoter site to inhibit transcription. Binds to the promoter of the vent2-B gene to activate transcription when in the presence of other BMP signaling factors also bound to the promoter. Inhibits the competence of ectodermal cells to respond to BMP during embryogenesis thereby inhibiting epidermal differentiation and promoting neural induction. Antagonizes the activity of nodal/activin signaling by forming a transcriptional repression complex on the gsc and mix2 gene promoters to inhibit their transcription, and thus maintain the undifferentiated state of embryonic cells to prevent them from differentiating prematurely. Acts maternally to inhibit vegt and beta-catenin-activated gene transcription by forming a transcriptional repression complex on the nodal/nr1 and siamois promoters to inhibit their transcription.6 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi321 – 38060HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: UniProtKB
  2. sequence-specific DNA binding transcription factor activity Source: InterPro
  3. SMAD binding Source: UniProtKB
  4. transcription factor binding Source: UniProtKB
  5. transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  1. gastrulation Source: UniProtKB
  2. negative regulation of activin receptor signaling pathway Source: UniProtKB
  3. negative regulation of BMP signaling pathway Source: UniProtKB
  4. negative regulation of epidermal cell differentiation Source: UniProtKB
  5. negative regulation of transcription, DNA-templated Source: UniProtKB
  6. positive regulation of transcription, DNA-templated Source: UniProtKB
  7. regulation of neurogenesis Source: UniProtKB
  8. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1.1
Alternative name(s):
POU class V protein oct-25Imported
Short name:
XOct-25Imported
Gene namesi
Name:pou5f1.1
Synonyms:oct-25Imported
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-919851. pou5f3.2.

Subcellular locationi

Nucleus By similarityPROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB
  2. transcription factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449POU domain, class 5, transcription factor 1.1PRO_0000390495Add
BLAST

Expressioni

Tissue specificityi

Highly enriched within the animal half of developing embryos within ectodermal and mesodermal regions. Expressed in the neuroectoderm at the early neurula stage, with expression initially extending to the future hindbrain/midbrain boundary, but later shifting toward the posterior pole where it persists within the tip of the tail in hatching embryos. Expressed at very low levels in the adult kidney.3 Publications

Developmental stagei

Expressed both maternally and zygotically. Expressed in oocytes and cleavage stage embryos. Expression increases during blastula stages, reaches a maximum at stage 11 during gastrulation, then subsequently declines to be no longer detectable by the end of neurulation.2 Publications

Interactioni

Subunit structurei

Interacts with components of the transcription complex that assembles on the vent2-B gene, including vent2 (via C-terminus), smad1 and smad4. Forms a repression complex on the promoters of the gsc and mix2 genes via interactions with the nodal/activin signaling pathway transducers foxh1/fast1, gtf2ird1/wbscr11 and smad2. Forms a repression complex on the promoters of the nodal/nr1 and siamois genes with the maternal factors tcf7l1/tcf3 and vegt.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
tcf7l1-bQ90ZB63EBI-7438970,EBI-7439087
vegt-aP873773EBI-7438970,EBI-7439000

Protein-protein interaction databases

IntActiQ7T103. 2 interactions.
MINTiMINT-4979275.

Structurei

3D structure databases

ProteinModelPortaliQ7T103.
SMRiQ7T103. Positions 230-379.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini227 – 30175POU-specificPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the POU transcription factor family. Class-5 subfamily.Sequence Analysis
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

HOVERGENiHBG053782.
KOiK09367.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7T103-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYSQQPFPAF AFNAGLMQDP ANCHFGGYTG LGHPQPFSFA FSTLKSENGE
60 70 80 90 100
SGVQGMGDCT TPVMPWNSLA SFDHQVQMEN NQQGNPPRAP SPTLSDSRIK
110 120 130 140 150
VKEEVVHETD SGEESPEPKY PSPPNPSLYY PNAWTGAPFW QVNPTPGNNI
160 170 180 190 200
NPMPNQTLVK NTSLPGNTTY PTPANQSPNT PVDCVTSSME SSRCSSTNSP
210 220 230 240 250
NGAINERATT IPNGEMLDGG QSSDNEEEVP SESEMEQFAK DLKHKRVSLG
260 270 280 290 300
YTQADVGYAL GVLYGKMFSQ TTICRFESLQ LSFKNMCQLK PFLERWVVEA
310 320 330 340 350
ENNDNLQELI NREQVIAQTR KRKRRTNIEN IVKGTLESYF MKCPKPGAQE
360 370 380 390 400
MVQIAKELNM DKDVVRVWFC NRRQKGKRQG MPTVEENDGE GYDVAQTMGS
410 420 430 440
PPVGHYALQQ VVTPQGYMAA PQIYASAFHK NDLFPQTVPH GMAMGGHIG
Length:449
Mass (Da):49,676
Last modified:October 1, 2003 - v1
Checksum:i38430BD13CF4115F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291T → A in AAA49996. (PubMed:1732736)Curated
Sequence conflicti71 – 711S → C in AAA49996. (PubMed:1732736)Curated
Sequence conflicti186 – 1861T → A in ABH07383. (PubMed:17950579)Curated
Sequence conflicti400 – 4012SP → R in AAA49996. (PubMed:1732736)Curated
Sequence conflicti428 – 4281F → G in AAA49996. (PubMed:1732736)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60074 mRNA. Translation: AAA49996.1.
AJ699165 mRNA. Translation: CAG27841.1.
DQ825761 mRNA. Translation: ABH07383.1.
BC055964 mRNA. Translation: AAH55964.1.
PIRiB42022.
RefSeqiNP_001079832.1. NM_001086363.1.
UniGeneiXl.4957.

Genome annotation databases

GeneIDi379522.
KEGGixla:379522.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60074 mRNA. Translation: AAA49996.1 .
AJ699165 mRNA. Translation: CAG27841.1 .
DQ825761 mRNA. Translation: ABH07383.1 .
BC055964 mRNA. Translation: AAH55964.1 .
PIRi B42022.
RefSeqi NP_001079832.1. NM_001086363.1.
UniGenei Xl.4957.

3D structure databases

ProteinModelPortali Q7T103.
SMRi Q7T103. Positions 230-379.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q7T103. 2 interactions.
MINTi MINT-4979275.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 379522.
KEGGi xla:379522.

Organism-specific databases

CTDi 379522.
Xenbasei XB-GENE-919851. pou5f3.2.

Phylogenomic databases

HOVERGENi HBG053782.
KOi K09367.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PRINTSi PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequential expression of multiple POU proteins during amphibian early development."
    Hinkley C.S., Martin J.F., Leibham D., Perry M.
    Mol. Cell. Biol. 12:638-649(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Gastrula1 Publication.
  2. "The POU factor Oct-25 regulates the Xvent-2B gene and counteracts terminal differentiation in Xenopus embryos."
    Cao Y., Knochel S., Donow C., Miethe J., Kaufmann E., Knochel W.
    J. Biol. Chem. 279:43735-43743(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH VENT2; SMAD1 AND SMAD4, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Gastrula1 Publication.
  3. "The Xenopus POU class V transcription factor XOct-25 inhibits ectodermal competence to respond to bone morphogenetic protein-mediated embryonic induction."
    Takebayashi-Suzuki K., Arita N., Murasaki E., Suzuki A.
    Mech. Dev. 124:840-855(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Tissue: Gastrula1 Publication.
  4. NIH - Xenopus Gene Collection (XGC) project
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: GastrulaImported.
  5. "Xenopus POU factors of subclass V inhibit activin/nodal signaling during gastrulation."
    Cao Y., Siegel D., Knochel W.
    Mech. Dev. 123:614-625(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos."
    Cao Y., Siegel D., Donow C., Knochel S., Yuan L., Knochel W.
    EMBO J. 26:2942-2954(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TCF7L1 AND VEGT, TISSUE SPECIFICITY.
  7. "Oct25 represses transcription of nodal/activin target genes by interaction with signal transducers during Xenopus gastrulation."
    Cao Y., Siegel D., Oswald F., Knochel W.
    J. Biol. Chem. 283:34168-34177(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FOXH1; GTF2IRD1 AND SMAD2.

Entry informationi

Entry nameiP5F11_XENLA
AccessioniPrimary (citable) accession number: Q7T103
Secondary accession number(s): A9CDQ2, Q99293
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: October 1, 2003
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3