Q7T0Z0 (SYMM_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase, mitochondrial
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase 2
Mitochondrial methionyl-tRNA synthetase
Short name=MtMetRS

Gene names

Name:

mars2

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 10	10	Mitochondrion Potential
	Chain	11 – 562	552	Methionine--tRNA ligase, mitochondrial		PRO_0000045496
Regions
	Motif	43 – 53	11	"HIGH" region
	Motif	332 – 336	5	"KMSKS" region
Sites
	Binding site	335	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7T0Z0 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: A6C04967DEC299F4	FASTA	562	63,461
	10 20 30 40 50 60 MLRSLALRTF ANILGLSSRS GSTAAMSRNA VASRPHLYTT PIFYVNAAPH LGHVYSALLA 70 80 90 100 110 120 DVQHRYSAMC GIESKLSTGT DEHGMKVQQA ASALGLDPQT FCSTVSLQFR TIFDALDISY 130 140 150 160 170 180 TDFVRTTEPR HIEAVSRFWM TLEEQGYIYK GTYEGWYCTS DEAFLSEGQT AEHTDFEGNK 190 200 210 220 230 240 IRVSLESGHQ VHWVSEENYM FRLSSLRPAL LNWLQTEPVH PAPFLKLVHH WLEEELPDLS 250 260 270 280 290 300 VSRQRSRLSW GIPVPSDSSH VIYVWLDALV NYLTAAGYPN PQLAPWGPST HLLGKDILRF 310 320 330 340 350 360 HAIYWPAFLI AAGLPPPQKL LVHSHWTSEG TKMSKSLKNV VDPSDCIRRY TTDGLRYYLL 370 380 390 400 410 420 RHGAPERDCD FTHRTARMLL NSELADALGG LLNRCTAPAI NPMQHFPKFQ YENFPVASRD 430 440 450 460 470 480 QVHDLLGALQ ELPVEVDQWI KKFQVHKALE CIDACVRRSN AFFQSQAPWK LQRGVEKEAA 490 500 510 520 530 540 LRDSVIYLTL EALRLYATLL HPAVPGLATV VLDRLGVPHK MRTLKKNTFL AATRGEICYF 550 560 QAQTLGPDKG LLFPRLEKSE AF

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC055977 mRNA. Translation: AAH55977.1.
RefSeq	NP_001079838.1. NM_001086369.1.
UniGene	Xl.12152.
3D structure databases
ProteinModelPortal	Q7T0Z0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	379528.
KEGG	xla:379528.
Organism-specific databases
CTD	92935.
Xenbase	XB-GENE-5862169. mars2.
Phylogenomic databases
HOVERGEN	HBG080702.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR014758. Met-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01874.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYMM_XENLA

Accession

Primary (citable) accession number: Q7T0Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program