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Q7T0T9 (PA24A_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytosolic phospholipase A2

Short name=cPLA2
Alternative name(s):
Phospholipase A2 group IVA

Including the following 2 domains:

  1. Phospholipase A2
    EC=3.1.1.4
    Alternative name(s):
    Phosphatidylcholine 2-acylhydrolase
  2. Lysophospholipase
    EC=3.1.1.5
Gene names
Name:pla2g4a
Synonyms:cpla2, pla2g4
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length749 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response By similarity.

Catalytic activity

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulation

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+ By similarity.

Subcellular location

Cytoplasm By similarity. Cytoplasmic vesicle By similarity. Note: Translocates to membrane vesicles in a calcium-dependent fashion By similarity.

Domain

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ By similarity.

Sequence similarities

Contains 1 C2 domain.

Contains 1 PLA2c domain.

Ontologies

Keywords
   Biological processLipid degradation
Lipid metabolism
   Cellular componentCytoplasm
Cytoplasmic vesicle
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
Gene Ontology (GO)
   Biological_processphospholipid catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasmic membrane-bounded vesicle

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionlysophospholipase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phospholipase A2 activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 749749Cytosolic phospholipase A2
PRO_0000345136

Regions

Domain5 – 106102C2
Domain138 – 740603PLA2c
Region1 – 178178Phospholipid binding By similarity

Sites

Active site2291Nucleophile By similarity
Active site5491Proton acceptor By similarity
Metal binding401Calcium 1 By similarity
Metal binding401Calcium 2 By similarity
Metal binding411Calcium 1; via carbonyl oxygen By similarity
Metal binding431Calcium 1 By similarity
Metal binding431Calcium 2 By similarity
Metal binding651Calcium 1 By similarity
Metal binding931Calcium 2 By similarity
Metal binding941Calcium 2; via carbonyl oxygen By similarity
Metal binding951Calcium 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7T0T9 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: CE769904144C4DE2

FASTA74985,282
        10         20         30         40         50         60 
MASIDPYQHI IVEHQYSHRF TVTVIKATNV TKGTFGDMLD TPDPYVELYI SSAPDSRKRT 

        70         80         90        100        110        120 
KHFNNNINPV WNETFEFILD PNQDNVLEIT LMDANYVMDE SLGTTTFPIL SVKPGEKKQV 

       130        140        150        160        170        180 
PFTFNKVTEM ILEFSLEVCS STDLRFSMAL CDQEKLFRQK RKNKVINGLR KLLGPEKTQD 

       190        200        210        220        230        240 
LNSTSRDVPV IAVLGSGGGF RAMIGFSGVM KALYESGVLD CATYVAGLSG STWYMSTLYS 

       250        260        270        280        290        300 
HPDFPTKGPK EINKELMHNV SYNPLLLLTP QKVKRYVEAL WKKKSSGQPV TFTDIFAMLI 

       310        320        330        340        350        360 
GETLIKDRMN RKLSHMQEKI NDGQCPLPLF TCLHVKPDVS ELMFADWVEF SPYEIGMAKY 

       370        380        390        400        410        420 
GTFMPPGLFG SKFFMGTVIK KYEENPLHFF MGVWGSAFSI LINRVLGVSN NSKGSTMEEE 

       430        440        450        460        470        480 
IENLKPKHIL GNDSSDSDDE MQEPKGTENA KAEEEYLRNN QASWVQRMLM AILGDSAIFN 

       490        500        510        520        530        540 
TREGRAGKVH NFMLGLNLNT SYPYSPLSGL CTQQSMEEDE LDAAVADPDE FEQIYEPLDV 

       550        560        570        580        590        600 
KSKKIHIVDS GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEL LLAEKWARMN 

       610        620        630        640        650        660 
KLPFPKIDPH VFDREGLKEC YIFKPKNTSV EKDCPTVIHF VLANLQFRNF KAPGVPRETT 

       670        680        690        700        710        720 
EEKESADFDI FDDPETPFST FNFQYPNVAF KQLHDLMEFN TLNNINVIKQ AMVESIEYRR 

       730        740 
QHPSRCSVSL NDVESRKLHH KDSQSKFQM 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Spleen.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC056041 mRNA. Translation: AAH56041.1.
RefSeqNP_001080867.1. NM_001087398.1.
UniGeneXl.29421.

3D structure databases

ProteinModelPortalQ7T0T9.
SMRQ7T0T9. Positions 13-721.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID380561.
KEGGxla:380561.

Organism-specific databases

CTD5321.
XenbaseXB-GENE-5838883. pla2g4a.

Phylogenomic databases

HOVERGENHBG053479.
KOK16342.

Family and domain databases

Gene3D2.60.40.150. 1 hit.
InterProIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePA24A_XENLA
AccessionPrimary (citable) accession number: Q7T0T9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2003
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families