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Protein

Cytosolic phospholipase A2

Gene

pla2g4a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response (By similarity).By similarity

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Calcium 1By similarity1
Metal bindingi40Calcium 2By similarity1
Metal bindingi41Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi43Calcium 1By similarity1
Metal bindingi43Calcium 2By similarity1
Metal bindingi65Calcium 1By similarity1
Metal bindingi93Calcium 2By similarity1
Metal bindingi94Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi95Calcium 2By similarity1
Active sitei229NucleophileBy similarity1
Active sitei549Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:pla2g4a
Synonyms:cpla2, pla2g4
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-5838883. pla2g4a.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmic vesicle By similarity

  • Note: Translocates to membrane vesicles in a calcium-dependent fashion.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003451361 – 749Cytosolic phospholipase A2Add BLAST749

Structurei

3D structure databases

ProteinModelPortaliQ7T0T9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 106C2PROSITE-ProRule annotationAdd BLAST102
Domaini138 – 740PLA2cPROSITE-ProRule annotationAdd BLAST603

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 178Phospholipid bindingBy similarityAdd BLAST178

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG053479.
KOiK16342.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7T0T9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASIDPYQHI IVEHQYSHRF TVTVIKATNV TKGTFGDMLD TPDPYVELYI
60 70 80 90 100
SSAPDSRKRT KHFNNNINPV WNETFEFILD PNQDNVLEIT LMDANYVMDE
110 120 130 140 150
SLGTTTFPIL SVKPGEKKQV PFTFNKVTEM ILEFSLEVCS STDLRFSMAL
160 170 180 190 200
CDQEKLFRQK RKNKVINGLR KLLGPEKTQD LNSTSRDVPV IAVLGSGGGF
210 220 230 240 250
RAMIGFSGVM KALYESGVLD CATYVAGLSG STWYMSTLYS HPDFPTKGPK
260 270 280 290 300
EINKELMHNV SYNPLLLLTP QKVKRYVEAL WKKKSSGQPV TFTDIFAMLI
310 320 330 340 350
GETLIKDRMN RKLSHMQEKI NDGQCPLPLF TCLHVKPDVS ELMFADWVEF
360 370 380 390 400
SPYEIGMAKY GTFMPPGLFG SKFFMGTVIK KYEENPLHFF MGVWGSAFSI
410 420 430 440 450
LINRVLGVSN NSKGSTMEEE IENLKPKHIL GNDSSDSDDE MQEPKGTENA
460 470 480 490 500
KAEEEYLRNN QASWVQRMLM AILGDSAIFN TREGRAGKVH NFMLGLNLNT
510 520 530 540 550
SYPYSPLSGL CTQQSMEEDE LDAAVADPDE FEQIYEPLDV KSKKIHIVDS
560 570 580 590 600
GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEL LLAEKWARMN
610 620 630 640 650
KLPFPKIDPH VFDREGLKEC YIFKPKNTSV EKDCPTVIHF VLANLQFRNF
660 670 680 690 700
KAPGVPRETT EEKESADFDI FDDPETPFST FNFQYPNVAF KQLHDLMEFN
710 720 730 740
TLNNINVIKQ AMVESIEYRR QHPSRCSVSL NDVESRKLHH KDSQSKFQM
Length:749
Mass (Da):85,282
Last modified:October 1, 2003 - v1
Checksum:iCE769904144C4DE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056041 mRNA. Translation: AAH56041.1.
RefSeqiNP_001080867.1. NM_001087398.1.
UniGeneiXl.29421.

Genome annotation databases

GeneIDi380561.
KEGGixla:380561.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056041 mRNA. Translation: AAH56041.1.
RefSeqiNP_001080867.1. NM_001087398.1.
UniGeneiXl.29421.

3D structure databases

ProteinModelPortaliQ7T0T9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi380561.
KEGGixla:380561.

Organism-specific databases

CTDi5321.
XenbaseiXB-GENE-5838883. pla2g4a.

Phylogenomic databases

HOVERGENiHBG053479.
KOiK16342.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA24A_XENLA
AccessioniPrimary (citable) accession number: Q7T0T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2003
Last modified: October 5, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.