Reviewed, UniProtKB/Swiss-Prot Q7SY53 (T23OB_DANRE)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase B
      Short name=TDO-B
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase B
      Short name=Tryptophanase B
    Tryptophan oxygenase B
      Short name=TRPO-B
      Short name=TO-B
    Tryptamin 2,3-dioxygenase B

Gene names

Name:	tdo2b
Synonyms:	tdo2, tdo2l
ORF Names:	zgc:63488

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Domain	Coiled coil
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tryptophan catabolic process to kynurenine Inferred from electronic annotation. Source: InterPro
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Tryptophan 2,3-dioxygenase B		PRO_0000247475
Regions
	Region	41 – 45	5	Substrate binding By similarity
	Region	71 – 75	5	Substrate binding By similarity
	Coiled coil	226 – 262	37	Potential
Sites
	Metal binding	327	1	Iron (heme axial ligand) By similarity
	Binding site	143	1	Substrate By similarity
	Binding site	150	1	Heme By similarity
	Binding site	341	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7SY53-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 8EA9740C0D4F3912	FASTA	407	47,422
	10 20 30 40 50 60 MSGCPFLGGT LQLLSSNPRQ AEEEDGSQGG VNKAAKGGII YGDYLQLDKV LNAQVLQSEQ 70 80 90 100 110 120 KGNKIHDEHL FIVTHQAYEL WFKQILWELD SVRDLFIKKH VRDERNMLKV VSRIHRITMI 130 140 150 160 170 180 FKLLVDQFAV LETMTALDFF DFREYLSPAS GFQSLQFRLL EQKIGVADHL RVPYNRRHYR 190 200 210 220 230 240 DNFHGEESET LLSSEQEPTL LQLVEQWLER TPGLEKDGFN FWGKLQANIE EGLKREKHQV 250 260 270 280 290 300 EKMEDTEVKQ ELLEDLNKQM ETFTALFDSK RHEHLLSKGE RRLSYKALQG ALMINFYREE 310 320 330 340 350 360 PRFQVPFQLL TALMEIDTLM TKWRYNHVCM VHRMIGSKAG TGGSSGYHYL RSTVSDRYKV 370 380 390 400 FVDLFNLATF LVPRSWVPKL NPNIHKFPYT AECYDSSYNS CSSEDSD

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AB.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC055123 mRNA. Translation: AAH55123.1.
IPI	IPI00492322.
RefSeq	NP_956150.1.
UniGene	Dr.80748
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSDARG00000023176. Danio rerio. [Contig view]
GeneID	334082.
KEGG	dre:334082.
Organism-specific databases
ZFIN	ZDB-GENE-030131-6014. tdo2b.
Phylogenomic databases
HOGENOM	Q7SY53.
HOVERGEN	Q7SY53.
Enzyme and pathway databases
BRENDA	1.13.11.11. 96826.
Gene expression databases
Bgee	Q7SY53.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23OB_DANRE

Accession

Primary (citable) accession number: Q7SY53

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project