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Protein

Protein kinase C beta type

Gene

prkcbb

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell survival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of card11/carma1, leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates isoform p66Shc of shc1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (andr)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domain.By similarity

Enzyme regulationi

Activated by diacylglycerol which in turn phosphorylates a range of cellular proteins.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi185 – 1851Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi186 – 1861Calcium 1By similarity
Metal bindingi186 – 1861Calcium 2By similarity
Metal bindingi192 – 1921Calcium 2By similarity
Metal bindingi245 – 2451Calcium 1By similarity
Metal bindingi245 – 2451Calcium 2By similarity
Metal bindingi246 – 2461Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi247 – 2471Calcium 1By similarity
Metal bindingi247 – 2471Calcium 2By similarity
Metal bindingi247 – 2471Calcium 3By similarity
Metal bindingi250 – 2501Calcium 3By similarity
Metal bindingi251 – 2511Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi253 – 2531Calcium 1By similarity
Metal bindingi253 – 2531Calcium 3By similarity
Binding sitei368 – 3681ATPPROSITE-ProRule annotation
Active sitei463 – 4631Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri35 – 8551Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri100 – 15051Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi345 – 3539ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Adaptive immunity, Apoptosis, Immunity, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DRE-114516. Disinhibition of SNARE formation.
R-DRE-1169091. Activation of NF-kappaB in B cells.
R-DRE-416993. Trafficking of GluR2-containing AMPA receptors.
R-DRE-4419969. Depolymerisation of the Nuclear Lamina.
R-DRE-5099900. WNT5A-dependent internalization of FZD4.
R-DRE-5218921. VEGFR2 mediated cell proliferation.
R-DRE-76005. Response to elevated platelet cytosolic Ca2+.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C beta type (EC:2.7.11.13)
Short name:
PKC-B
Short name:
PKC-beta
Gene namesi
Name:prkcbb
Synonyms:prkcb1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 3

Organism-specific databases

ZFINiZDB-GENE-040426-1178. prkcbb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 670669Protein kinase C beta typePRO_0000394258Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei497 – 4971PhosphothreonineBy similarity
Modified residuei501 – 5011PhosphothreonineBy similarity
Modified residuei631 – 6311Phosphothreonine; by autocatalysisBy similarity
Modified residuei638 – 6381Phosphothreonine; by autocatalysisBy similarity
Modified residuei657 – 6571Phosphoserine; by autocatalysisBy similarity

Post-translational modificationi

Phosphorylation on Thr-497 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-638 maintains catalytic competence, and autophosphorylation on Ser-657 appears to release the kinase into the cytosol (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7SY24.
PRIDEiQ7SY24.

Expressioni

Gene expression databases

BgeeiQ7SY24.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000038918.

Structurei

3D structure databases

ProteinModelPortaliQ7SY24.
SMRiQ7SY24. Positions 36-287, 336-663.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini157 – 259103C2PROSITE-ProRule annotationAdd
BLAST
Domaini339 – 597259Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini598 – 66770AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri35 – 8551Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri100 – 15051Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0694. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126964.
HOGENOMiHOG000233022.
HOVERGENiHBG108317.
InParanoidiQ7SY24.
KOiK19662.
OMAiLTKNPQK.
OrthoDBiEOG77M8QM.
PhylomeDBiQ7SY24.
TreeFamiTF351133.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014375. Protein_kinase_C_a/b/g.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000550. PKC_alpha. 1 hit.
PRINTSiPR00360. C2DOMAIN.
PR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7SY24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPANSDGE ERSAPMRGFA RQGALRQKNV HEVKNHKFIA RFFKQPTFCS
60 70 80 90 100
HCTDFIWGFG KQGFQCQVCC FVVHKRCHEF VTFSCPGADK GPASDDPRSK
110 120 130 140 150
HKFKVHTYSS PTFCDHCGSL LYGLIHQGMR CDHCMMNIHK RCVANVPSLC
160 170 180 190 200
GTDHTERRGR IQITAEIKNN VLTVSIKEAK NLVPMDPNGL SDPYVKLKLI
210 220 230 240 250
PDPKSESKQK TKTIKCCLNP TWNETFTFNL KESDKDRRLS VEIWDWDLTS
260 270 280 290 300
RNDFMGSLSF GISELQKQGV DGWFKLLSQE EGEYFNVPVP PEGEEGNEEL
310 320 330 340 350
RQKFERAKIG PSKTDGSSSN AISKFDSNGN RDRMKLSDFN FLMVLGKGSF
360 370 380 390 400
GKVMLAERKG ADELFAIKIL KKDVVIQDDD VECTMVEKRV LALSGKPPFL
410 420 430 440 450
TQLHSCFQTM DRLYFVMEYI NGGDLMYHIQ QVGKFKEPHA VFYAAEIAIG
460 470 480 490 500
LFFLHSKGVI YRDLKLDNVM LDAEGHIKIA DFGMCKENML DGVTTKTFCG
510 520 530 540 550
TPDYIAPEII AYQPYGKSVD WWAFGVLLYE MLAGQPPFDG EDEDELFQSI
560 570 580 590 600
MEHHVSYPKS MSKEAVAICK GLMTKHPGKR LGCGPEGERD IREHGFFRYM
610 620 630 640 650
DWEKLEHREV QPPFKPKACG RDAENFDRFF TRHPPVLTPP DQEVIMNLDQ
660 670
DEFEGFSFIN PEFPAMEAQS
Length:670
Mass (Da):76,306
Last modified:October 1, 2003 - v1
Checksum:iDF2D78CFF9BDDCA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055154 mRNA. Translation: AAH55154.1.
RefSeqiNP_957272.1. NM_200978.1.
UniGeneiDr.84921.

Genome annotation databases

EnsembliENSDART00000029451; ENSDARP00000038918; ENSDARG00000022254.
GeneIDi393953.
KEGGidre:393953.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055154 mRNA. Translation: AAH55154.1.
RefSeqiNP_957272.1. NM_200978.1.
UniGeneiDr.84921.

3D structure databases

ProteinModelPortaliQ7SY24.
SMRiQ7SY24. Positions 36-287, 336-663.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000038918.

Proteomic databases

PaxDbiQ7SY24.
PRIDEiQ7SY24.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000029451; ENSDARP00000038918; ENSDARG00000022254.
GeneIDi393953.
KEGGidre:393953.

Organism-specific databases

CTDi393953.
ZFINiZDB-GENE-040426-1178. prkcbb.

Phylogenomic databases

eggNOGiKOG0694. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126964.
HOGENOMiHOG000233022.
HOVERGENiHBG108317.
InParanoidiQ7SY24.
KOiK19662.
OMAiLTKNPQK.
OrthoDBiEOG77M8QM.
PhylomeDBiQ7SY24.
TreeFamiTF351133.

Enzyme and pathway databases

ReactomeiR-DRE-114516. Disinhibition of SNARE formation.
R-DRE-1169091. Activation of NF-kappaB in B cells.
R-DRE-416993. Trafficking of GluR2-containing AMPA receptors.
R-DRE-4419969. Depolymerisation of the Nuclear Lamina.
R-DRE-5099900. WNT5A-dependent internalization of FZD4.
R-DRE-5218921. VEGFR2 mediated cell proliferation.
R-DRE-76005. Response to elevated platelet cytosolic Ca2+.

Miscellaneous databases

NextBioi20814925.
PROiQ7SY24.

Gene expression databases

BgeeiQ7SY24.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014375. Protein_kinase_C_a/b/g.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000550. PKC_alpha. 1 hit.
PRINTSiPR00360. C2DOMAIN.
PR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiKPCB_DANRE
AccessioniPrimary (citable) accession number: Q7SY24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: October 1, 2003
Last modified: May 11, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.