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Protein

Protein kinase C beta type

Gene

prkcbb

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell survival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of card11/carma1, leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates isoform p66Shc of shc1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (andr)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domain.By similarity

Enzyme regulationi

Activated by diacylglycerol which in turn phosphorylates a range of cellular proteins.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi185Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi186Calcium 1By similarity1
Metal bindingi186Calcium 2By similarity1
Metal bindingi192Calcium 2By similarity1
Metal bindingi245Calcium 1By similarity1
Metal bindingi245Calcium 2By similarity1
Metal bindingi246Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi247Calcium 1By similarity1
Metal bindingi247Calcium 2By similarity1
Metal bindingi247Calcium 3By similarity1
Metal bindingi250Calcium 3By similarity1
Metal bindingi251Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi253Calcium 1By similarity1
Metal bindingi253Calcium 3By similarity1
Binding sitei368ATPPROSITE-ProRule annotation1
Active sitei463Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri35 – 85Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri100 – 150Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51
Nucleotide bindingi345 – 353ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Kinase, Serine/threonine-protein kinase, Transferase
Biological processAdaptive immunity, Apoptosis, Immunity, Transcription, Transcription regulation
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DRE-114516 Disinhibition of SNARE formation
R-DRE-1169091 Activation of NF-kappaB in B cells
R-DRE-416993 Trafficking of GluR2-containing AMPA receptors
R-DRE-4419969 Depolymerisation of the Nuclear Lamina
R-DRE-5099900 WNT5A-dependent internalization of FZD4
R-DRE-5218921 VEGFR2 mediated cell proliferation
R-DRE-76005 Response to elevated platelet cytosolic Ca2+

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C beta type (EC:2.7.11.13)
Short name:
PKC-B
Short name:
PKC-beta
Gene namesi
Name:prkcbb
Synonyms:prkcb1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 3

Organism-specific databases

ZFINiZDB-GENE-040426-1178 prkcbb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003942582 – 670Protein kinase C beta typeAdd BLAST669

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei497PhosphothreonineBy similarity1
Modified residuei501PhosphothreonineBy similarity1
Modified residuei631Phosphothreonine; by autocatalysisBy similarity1
Modified residuei638Phosphothreonine; by autocatalysisBy similarity1
Modified residuei657Phosphoserine; by autocatalysisBy similarity1

Post-translational modificationi

Phosphorylation on Thr-497 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-638 maintains catalytic competence, and autophosphorylation on Ser-657 appears to release the kinase into the cytosol (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7SY24
PRIDEiQ7SY24

Expressioni

Gene expression databases

BgeeiENSDARG00000022254

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000038918

Structurei

3D structure databases

ProteinModelPortaliQ7SY24
SMRiQ7SY24
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini157 – 259C2PROSITE-ProRule annotationAdd BLAST103
Domaini339 – 597Protein kinasePROSITE-ProRule annotationAdd BLAST259
Domaini598 – 667AGC-kinase C-terminalAdd BLAST70

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri35 – 85Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri100 – 150Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0694 Eukaryota
ENOG410XNPH LUCA
GeneTreeiENSGT00820000126964
HOGENOMiHOG000233022
HOVERGENiHBG108317
InParanoidiQ7SY24
KOiK19662
OMAiQAHIDRE
OrthoDBiEOG091G0QRS
PhylomeDBiQ7SY24
TreeFamiTF351133

Family and domain databases

CDDicd00029 C1, 2 hits
cd05616 STKc_cPKC_beta, 1 hit
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR034664 cPKC_beta
IPR020454 DAG/PE-bd
IPR011009 Kinase-like_dom_sf
IPR002219 PE/DAG-bd
IPR017892 Pkinase_C
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR014375 Protein_kinase_C_a/b/g
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF00168 C2, 1 hit
PF00069 Pkinase, 1 hit
PF00433 Pkinase_C, 1 hit
PIRSFiPIRSF000550 PKC_alpha, 1 hit
PRINTSiPR00360 C2DOMAIN
PR00008 DAGPEDOMAIN
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00239 C2, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS50004 C2, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7SY24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPANSDGE ERSAPMRGFA RQGALRQKNV HEVKNHKFIA RFFKQPTFCS
60 70 80 90 100
HCTDFIWGFG KQGFQCQVCC FVVHKRCHEF VTFSCPGADK GPASDDPRSK
110 120 130 140 150
HKFKVHTYSS PTFCDHCGSL LYGLIHQGMR CDHCMMNIHK RCVANVPSLC
160 170 180 190 200
GTDHTERRGR IQITAEIKNN VLTVSIKEAK NLVPMDPNGL SDPYVKLKLI
210 220 230 240 250
PDPKSESKQK TKTIKCCLNP TWNETFTFNL KESDKDRRLS VEIWDWDLTS
260 270 280 290 300
RNDFMGSLSF GISELQKQGV DGWFKLLSQE EGEYFNVPVP PEGEEGNEEL
310 320 330 340 350
RQKFERAKIG PSKTDGSSSN AISKFDSNGN RDRMKLSDFN FLMVLGKGSF
360 370 380 390 400
GKVMLAERKG ADELFAIKIL KKDVVIQDDD VECTMVEKRV LALSGKPPFL
410 420 430 440 450
TQLHSCFQTM DRLYFVMEYI NGGDLMYHIQ QVGKFKEPHA VFYAAEIAIG
460 470 480 490 500
LFFLHSKGVI YRDLKLDNVM LDAEGHIKIA DFGMCKENML DGVTTKTFCG
510 520 530 540 550
TPDYIAPEII AYQPYGKSVD WWAFGVLLYE MLAGQPPFDG EDEDELFQSI
560 570 580 590 600
MEHHVSYPKS MSKEAVAICK GLMTKHPGKR LGCGPEGERD IREHGFFRYM
610 620 630 640 650
DWEKLEHREV QPPFKPKACG RDAENFDRFF TRHPPVLTPP DQEVIMNLDQ
660 670
DEFEGFSFIN PEFPAMEAQS
Length:670
Mass (Da):76,306
Last modified:October 1, 2003 - v1
Checksum:iDF2D78CFF9BDDCA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055154 mRNA Translation: AAH55154.1
RefSeqiNP_957272.1, NM_200978.1
UniGeneiDr.84921

Genome annotation databases

EnsembliENSDART00000029451; ENSDARP00000038918; ENSDARG00000022254
GeneIDi393953
KEGGidre:393953

Similar proteinsi

Entry informationi

Entry nameiKPCB_DANRE
AccessioniPrimary (citable) accession number: Q7SY24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: October 1, 2003
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health