Q7SY23 (AL4A1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
Short name=P5C dehydrogenase
EC=1.5.1.12

Alternative name(s):
Aldehyde dehydrogenase family 4 member A1

Gene names

Name:	aldh4a1
ORF Names:	zgc:63592

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles By similarity.
Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Proline metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 17	17	Mitochondrion By similarity
	Chain	18 – 556	539	Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial		PRO_0000007175
Regions
	Nucleotide binding	289 – 294	6	NAD By similarity
Sites
	Active site	307	1	By similarity
	Active site	341	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7SY23 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: E7973E9787CEA358	FASTA	556	61,587
	10 20 30 40 50 60 MLRARSAVSQ SWKGFKTFSC VAVEVKNEPV LEFKEGSKER AELEEALRNL KGKTEEIPCV 70 80 90 100 110 120 IGNEEVWTKD IRFQLSPFNH SHQVAKFCYA DKDLLNKAIE ASVAARREWD LKPVSDRAQI 130 140 150 160 170 180 FFKAADIISG PKRAEVLAKT MIGQGKTVVQ AEIDAAPELI DFFRFNAKHA IELEDQQPLD 190 200 210 220 230 240 SDGSTNTMLY RGLEGFVAAV APFNFTAIGG NLAGTPALMG NVVLWKPSDT AMSASYAVYK 250 260 270 280 290 300 ILRESGLPPN IIQFVPADGP VFGDTVTSSE HLAGINFTGS VPTFKRLWKQ VAQNLDIYKN 310 320 330 340 350 360 FPRVAGECGG KNFHFVHKSA DVRSVVTGTI RSAFEYGGQK CSACSRMYVP DSLWPQIRQG 370 380 390 400 410 420 LLDVYKQIKV GDPVEDFSTF FSAVIDDKSF SRIKGWLEHA RSSPHLKIIA GGNCDDKKGY 430 440 450 460 470 480 FVEPTIIETT DPQEKIMNEE IFGPVLTVYV YPENDYKKVL HLIDNTSPYA LTGAIFPQDK 490 500 510 520 530 540 SVIEEAGKAL RNAAGNYYIN DKSTGSIVAQ QPFGGARASG TNDKPGGPHY VLRWTSPQVV 550 KQTHVPLTEW KYPYMS

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: SJD.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC055155 mRNA. Translation: AAH55155.1.
IPI	IPI00487440.
RefSeq	NP_957452.1. NM_201158.1.
UniGene	Dr.84922.
3D structure databases
ProteinModelPortal	Q7SY23.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7SY23.
Proteomic databases
PRIDE	Q7SY23.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	394133.
KEGG	dre:394133.
Organism-specific databases
CTD	8659.
ZFIN	ZDB-GENE-040426-1179. aldh4a1.
Phylogenomic databases
eggNOG	fiNOG09028.
GeneTree	ENSGT00560000077335.
HOGENOM	HBG752218.
HOVERGEN	HBG050484.
InParanoid	Q7SY23.
OrthoDB	EOG4ZCT3Q.
PhylomeDB	Q7SY23.
Gene expression databases
ArrayExpress	Q7SY23.
Bgee	Q7SY23.
Family and domain databases
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR005931. Delta1-pyrroline-5-COlate_DH-1. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
KO	K00294.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR01236. D1pyr5carbox1. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AL4A1_DANRE

Accession

Primary (citable) accession number: Q7SY23

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	October 1, 2003
Last modified:	November 16, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program