Q7SXZ1 (PIGB_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GPI mannosyltransferase 3
EC=2.4.1.-

Alternative name(s):
GPI mannosyltransferase III
Short name=GPI-MT-III
Phosphatidylinositol-glycan biosynthesis class B protein
Short name=PIG-B

Gene names

Name:	pigb
ORF Names:	zgc:63658

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third alpha-1,2-mannose to Man2-GlcN-acyl-PI during GPI precursor assembly By similarity.
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 22 family. PIGB subfamily.

Ontologies

Keywords
Biological process	GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	GPI mannosyltransferase 3		PRO_0000246253
Regions
	Transmembrane	40 – 60	21	Helical; Potential
	Transmembrane	118 – 138	21	Helical; Potential
	Transmembrane	206 – 226	21	Helical; Potential
	Transmembrane	243 – 263	21	Helical; Potential
	Transmembrane	297 – 317	21	Helical; Potential
	Transmembrane	322 – 342	21	Helical; Potential
	Transmembrane	344 – 364	21	Helical; Potential
	Transmembrane	369 – 389	21	Helical; Potential
Amino acid modifications
	Glycosylation	15	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Glycosylation	467	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7SXZ1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: B16C0394921DE814	FASTA	536	62,240
	10 20 30 40 50 60 MEKIRERFKQ SKSSNESVRL RKRSSILYST DDRKLQTLKI FGINITVFIV LVRLLNCVLV 70 80 90 100 110 120 QTSFVPDEFW QSLEISHRMV FGYGYETWEW KEGIRGYSYP LLFALMYKLL QLISYDTVYL 130 140 150 160 170 180 LVFVPRVFQA LLAAYADVKL YKLILQWETP DVAKWTCFCQ LCSWFAWFCC SRTLTNTTET 190 200 210 220 230 240 ALTTLALCFY PLPGSKTHSS WKYLTLVSLA VVIRPTALIV WFPLIFYHFC TDQDKLKLIT 250 260 270 280 290 300 HRYFPIGVLA LGVSTLIDSF FYGKWIFVQW NFLKFNVLHN VGEFYGSHPW HWYWTQGLPV 310 320 330 340 350 360 VIGPHLPLVL HGCLLSTRKH TILLLTIIWT TAVYSLLAHK EFRFIYPVLP FCMIFCGLSL 370 380 390 400 410 420 AKLQAWRKAA AGALLLFNLC PALYTGLVHQ RGALDVMHVL QPLCVSSQSP NPPELLFLMP 430 440 450 460 470 480 CHSTPLYSHL HCPLKLRFLE CPPDLTGRQD YMDEADVFFS DPLHWLNTSF PLRSTLPSHL 490 500 510 520 530 VLFDSLQKEI SSFLEENSFA KQAEIFHTHF PEGRVGKNIL IYSRRSEMKI NEGFIL

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: SJD.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC055191 mRNA. Translation: AAH55191.1.
IPI	IPI00489653.
RefSeq	NP_956770.1. NM_200476.1.
UniGene	Dr.17204.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7SXZ1.
Protein family/group databases
CAZy	GT22. Glycosyltransferase Family 22.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	393448.
KEGG	dre:393448.
Organism-specific databases
ZFIN	ZDB-GENE-040426-1216. zgc:63658.
Phylogenomic databases
eggNOG	fiNOG10194.
GeneTree	ENSGT00550000075012.
HOVERGEN	HBG082136.
InParanoid	Q7SXZ1.
PhylomeDB	Q7SXZ1.
Gene expression databases
ArrayExpress	Q7SXZ1.
Bgee	Q7SXZ1.
Family and domain databases
InterPro	IPR005599. GPI_mannosylTrfase. [Graphical view]
KO	K05286.
PANTHER	PTHR22760. Alg9_trans. 1 hit.
Pfam	PF03901. Glyco_transf_22. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIGB_DANRE

Accession

Primary (citable) accession number: Q7SXZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	October 1, 2003
Last modified:	November 16, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program